Effect of ultrasonic agitation on the release of copper, iron, manganese and zinc from soil and sediment using the BCR three-stage sequential extraction

An ultrasonic bath and an ultrasonic probe have been used to develop rapid versions of the three-stage Community Bureau of Reference (BCR, now the Standards, Measurement and Testing Programme) sequential extraction procedure. The effect of the ultrasonic treatments on the extraction of copper, iron, manganese and zinc from a sewage sludge-amended soil has been assessed. Recoveries similar to those of conventional shaking (i.e., conventional value, +/- 30%) could generally be obtained for copper, manganese and zinc, but not for the important matrix element iron. With the use of compromise sonication conditions, steps 1, 2 and 3 of the sequential extraction (excluding the hydrogen peroxide digestion in step 3, which was not performed with sonication) could be completed in 3, 5 and 1 min, respectively, using the ultrasonic probe, and in 3, 1 and 1 h, respectively, using the bath. The extraction procedures developed using the soil performed well when applied to lake sediment BCR CRM 601. Analyte partitioning was generally similar to that obtained with mechanical shaking, and overall metal recoveries were 84-98% of those obtained with the conventional BCR protocol, except for copper extracted with the probe (74%). Poorer performance (analyte recoveries, 58-104%) was obtained when the methods were applied to an intertidal sediment. This highlights the difficulty of developing a version of the BCR extraction, with ultrasonic assistance, which gives a performance equivalent to conventional shaking when applied to different substrates.     

Characterising strongly advected discharges in the initial dilution zone

Mean concentration fields of strongly advected non-buoyant discharges are characterised with a double-Gaussian assumption. Comparisons with experimental data show that the approximation provides a reasonable representation of the cross-sectional profiles. The self-similarity of these profiles enables their form to be represented by two additional parameters, one describing the relative separation of the peaks and the other the ratio of the cross-sectional spreads. Values for these additional parameters are determined from experimental data. This systematic approach to characterising the strongly advected flows provides a consistent framework for determining spreading rates and concentration ratios, such as the peak to centreline maximum and the peak to top hat. The double-Gaussian framework also provides a basis for comparisons with the CorJet and VisJet numerical models. In addition the double-Gaussian assumption is employed to interpret data obtained using the Light Attenuation technique. This is a relatively simple measuring system, which provides depth integrated concentration information. The data obtained using this technique is shown to be generally consistent with that from previous studies.     

Coastal directories and beyond: providing multidisciplinary coastal zone resource information for resource management

Baseline resource information in an easily accessible form is a vital starting point for developing coastal zone management. On behalf of a wide-ranging group of organizations involved in the management of the U.K. maritime zone, the Joint Nature Conservation Committee (JNCC) is compiling a series of 16 regional volumes. The volumes contain multi-disciplinary coastal zone resource information arranged in ten chapters under the general title ‘Coasts and Seas of the UK’. These volumes form part of a series of paper and electronic publications of coastal information being produced by JNCC’s Coastal Directories Project and are designed to meet the needs of planners and all others involved in decision-making and management of the coast.     

A new model for the CBL growth based on the turbulent kinetic energy equation

Starting from the evolution equation for the turbulent energy density spectrum (EDS), we develop a new model for the growth of the Convective boundary layer (CBL). We apply dimensional analysis to parameterize the unknown inertial transport and convective source term in the dynamic equation for the three-dimensional (3-D) spectrum and solve the 3-D EDS equation. The one-dimensional vertical spectrum is derived from the 3-D spectrum, employing a weight function. This allows us to select the magnitude of the vertical spectral component for the construction of the growing 3-D EDS. Furthermore, we employ the vertical component of the energy spectrum to calculate the eddy diffusivity (required in dispersion models). Currently there are no available experimental data to directly verify our EDS model.     

Multiple stressors and amphibian declines: dual impacts of pesticides and fish on yellow-legged frogs.

More than 40% of Earth's 5700+ amphibian species have undergone recent declines. Despite the likely involvement of multiple factors in driving these declines, most studies continue to focus on single stressors. In California (USA), separate studies have implicated either introduced fish or pesticides as causal agents. To date, however, no study has simultaneously evaluated the respective roles of these two potential stressors nor attempted to assess their relative importance, information critical for the development of effective conservation efforts and environmental policies. We examined the role and relative effect of fish and pesticides on the mountain yellow-legged frog (Rana muscosa) using unusually detailed data sets for a large portion of R. muscosa's historic range in California's Sierra Nevada. Habitat characteristics and presence/absence of R. muscosa and fish were quantified at each of 6831 sites during field surveys. Pesticide use upwind of each site was calculated from pesticide application records and predominant wind directions. Using generalized additive models, we found that, after accounting for habitat effects, the probability of R. muscosa presence was significantly reduced by both fish and pesticides, with the landscape-scale effect of pesticides much stronger than that of fish. The degree to which a site was sheltered from the predominant wind (and associated pesticides) was also a significant predictor of R. muscosa presence. Taken together, these results represent the strongest evidence to date that windborne pesticides are contributing to amphibian declines in pristine locations. Our results suggest that amphibian declines may have complex multi-factorial causes, and caution that single-factor studies that demonstrate the importance of one factor should not be used as evidence against the importance of other factors.     

Effects of UV-B irradiation on growth and survival of Antarctic marine diatoms

Growth rate, survival, and stimulation of the production of UV-B (280 to 320 nm) absorbing compounds were investigated in cultures of five commonly occurring Antarctic marine diatoms exposed to a range of UV-B irradiances. Experimental UV-B exposures ranged from 20 to 650% of the measured peak surface irradiance at an Antarctic coastal site (0.533 J m^-2 s^-1). The five diatom species (Nitzschia lecointei, Proboscia alata, P. inermis, Thalassiosira tumida and Stellarima microtrias) appear capable of surviving two to four times this irradiance. In contrast to Phaeocystis cf. pouchetii, another major component of the Antarctic phytoplankton, the concentrations of pigments with discrete UV absorption peaks in diatoms were low and did not change significantly under increasing UV-B irradiance. Absorbance of UV-B by cells from which pigments had been extracted commonly greatly exceeded that of the pigments themselves. Most of this absorbance was due to oxidisable cell contents, with the frustule providing the remainder. Survival of diatoms did not correlate with absorption by either pigments, frustules or oxidisable cell contents, indicating that their survival under elevated UV-B irradiances results from processes other than screening mechanisms.     

Stratified flow interactions with a suspended canopy

Field observations of the interactions between a stratified flow and a canopy suspended from the free surface above a solid boundary are described and analysed. Data were recorded in and around the canopy formed by a large long-line mussel farm. The canopy causes a partial blockage of the water flow, reducing velocities in the upper water column. Deceleration of the approaching flow results in a deepening of isopycnals upstream of the canopy. Energy considerations show that the potential for an approaching stratified flow to be diverted beneath a porous canopy is indicated by a densimetric Froude number. Strong stratification or low-velocities inhibit vertical diversion beneath the canopy, instead favouring a horizontal diversion around the sides. The effect on vertical mixing is also considered with a shear layer generated beneath the canopy and turbulence generated from drag within the canopy. In the observations, stratification is shown to be of sufficient strength to limit the effectiveness of the first mixing process, while the turbulence within the canopy is likely to enhance vertical exchange. Velocity and temperature microstructure measurements are used to investigate the effect of the canopy on turbulent dissipation and show that dissipation is enhanced within the canopy.     

The role of herbicides in the erosion of salt marshes in eastern England

Laboratory studies and field trials were conducted to investigate the role of herbicides on saltmarsh vegetation, and their possible significance to saltmarsh erosion. Herbicide concentrations within the ranges present in the aquatic environment were found to reduce the photosynthetic efficiency and growth of both epipelic diatoms and higher saltmarsh plants in the laboratory and in situ. The addition of sublethal concentrations of herbicides resulted in decreased growth rates and photosynthetic efficiency of diatoms and photosynthetic efficiency of higher plants. Sediment stability also decreased due to a reduction in diatom EPS production. There was qualitative evidence that diatoms migrated deeper into the sediment when the surface was exposed to simazine, reducing surface sediment stability by the absence of a cohesive biofilm. Sediment loads on leaves severely reduced photosynthesis in Limonium vulgare. This, coupled with reduced carbon assimilation from the effects of herbicides, could have large negative consequences for plant productivity and over winter survival of saltmarsh plants. The data support the hypothesis that sublethal herbicide concentrations could be playing a role in the increased erosion of salt marshes that has occurred over the past 40 years.     

Nutrient and mercury variations in soils from family farms of the Tapajós region (Brazilian Amazon): Recommendations for better farming

In the Brazilian Amazon, colonization is modifying the landscape at an exceedingly fast pace. Recently established households practice slash-and-burn agriculture and participate in the overall deforestation of the Amazon. Near the Transamazon highway, these family agricultural practices are the main cause of deforestation. The study presented here is oriented toward a better understanding of the impacts of farming practices on soil chemical composition. This study used a sampling design based on soil samples taken on farm plots, which had been submitted to a wide range of spatial and temporal sequential land-uses, including soils that were only recently denuded. The data shows that soil responses (organic matter (OM) content, fertility and mercury (Hg) retention) to these varied land-uses were relatively similar, suggesting that the most important event determining the responses was deforestation itself. This is well illustrated by the Hg content of soils, which changed immediately after deforestation and then only slightly thereafter. This phenomenon could also be seen in the base cation (calcium (Ca), potassium (K) and magnesium (Mg)) content which rose drastically after deforestation and tended to stay high for a period up to 10 years of cropping and pasture. This lasting cation rise is reflected by ammonium (NH₄) displacement from surface soils. Indeed, inorganic nitrogen (N) is the most important nutrient loss upon deforestation. Nonetheless, when time spent in fallow was greater than 15 years, base cations (Ca, Mg, K), available N and phosphorus (P) contents tended to go back to initial forest soil values and in some cases to exceed them. Soil type was seen to mediate responses to land-use. Clay-sandy soils showed a lower content of available N and carbon (C) than clayey soils at the soil surface, a difference that was accentuated by deforestation. Conversely, the higher initial content of Hg in clayey soils was associated with a more important Hg loss from the soil's surface. By shedding light on the consequences of family practices for OM, nutrient status and Hg depletion, this paper gives a new perspective on soil responses to agricultural practices. These conclusions need to be addressed in a strategy plan to limit family land-use impacts on soils and the surrounding ecosystems. Recommendations for more sustainable land uses are proposed based on what has been learned about soil responses to local agricultural practices.     

A misdiagnosis of X-linked adrenoleucodystrophy in cultured chorionic villus cells by the measurement of very long chain fatty acids

A case is reported of a male fetus at risk of X-linked adrenoleucodystrophy who showed a normal cultured chorionic villus cell very long chain fatty acid (VLCFA) profile but at birth exhibited grossly abnormal plasma and cultured fibroblast VLCFAs. Maternal contamination or a sample mix-up was excluded by chromosome analysis and analysis of polymorphic markers. This is the second report of a fetus affected with this disorder who showed normal cultured chorionic villus cell VLCFAs. It highlights the importance of a proper audit of all prenatal diagnoses to evaluate method reliability.