Diagnosis of Bloom's syndrome by sister chromatid exchange evaluation in chorionic villus cultures

Cultures of a chorion biopsy taken from a pregnancy at risk of Bloom's syndrome revealed the high sister chromatid exchange frequency diagnostic of this rare disorder. To obtain the result, cultures were grown under standard conditions, with the addition of 10μM 5′;-bromodeoxyuridine for the final 48 h of incubation. This result demonstrates the feasibility of early prenatal diagnosis of Bloom's syndrome.     

Evidence for covalently bonded chlorine–fullerene formed by ozonation and chlorination at room temperature

This article reports for the first time that fullerene (nC₆₀) can form chlorinated disinfection by-products in aqueous systems at ambient temperature. The ability of nC₆₀ to form colloidal suspensions in aqueous media increases the chance that these particles will migrate in the environment and then in drinking water supply systems. Since nC₆₀ is not completely removed by conventional water treatment, any residual nC₆₀ is likely to be oxidized during disinfection process. While the ozonation of nC₆₀ has been studied, little is known about the reaction between nC₆₀ and chlorine. To address this issue, we subjected aqueous nC₆₀ suspensions to chlorination and sequential ozonation/chlorination at ozone dosages of 4.5, 10, 15 and 24 mg O₃/mg nC₆₀. The morphology and physicochemical properties of oxidized nC₆₀ aggregates were evaluated by scanning electron microscopy, transmission electron microscopy, UV–visible absorption spectroscopy and X-ray photoelectron spectroscopy (XPS). We found that while the particles in the as-prepared nC₆₀ were predominantly spheres, the ozonation of nC₆₀ resulted in the formation of irregularly shaped aggregates. The concentration of atomic carbon found by XPS in the nC₆₀ samples decreased from 92 % for the as-prepared nC₆₀ to 50 % for the aggregates ozonated at 24 mg O₃/mg nC₆₀ and then chlorinated at 68 mg Cl₂/L and allowed to react for 100 min. The presence of Cl atoms covalently bonded to C atoms was confirmed by XPS peaks corresponding to a binding energy (E _b) of 200.1–202.4 eV. This demonstrates the need to better assess and monitor the formation of potentially toxic chlorinated disinfection by-products from carbon nanomaterials during water treatment.     

Sustainable construction: composite use of tyres and ash in concrete

An investigation was carried out to establish the physical, mechanical and chemical characteristics of a non-standard (unprocessed) pulverised fuel ash (PFA) and waste tyres from a former landfill site at the Power Station Hill near Church Village, South Wales, United Kingdom. Investigations are on-going to establish the suitability of the fly ash and/or tyres in road construction (embankment and pavement) and also in concrete to be used in the construction of the proposed highway. This paper reports on concrete-based construction where concrete blends (using various levels of PFA as partial replacement for Portland cement (PC), and shredded waste tyres (chips 15-20mm) as aggregate replacement) were subjected to unconfined compressive strength tests to establish performance, hence, optimising mix designs. Strength development up to 180 days for the concrete made with PC-PFA blends as binders (PC-PFA concrete), with and without aggregate replacement with tyre chips, is reported. The binary PC-PFA concrete does not have good early strength but tends to improve at longer curing periods. The low early strength observed means that PC-PFA concrete cannot be used for structures, hence, only as low to medium strength applications such as blinding, low-strength foundations, crash barriers, noise reduction barriers, cycle paths, footpaths and material for pipe bedding.     

Estimation of septic tank setback distances based on transport of E. coli and F-RNA phages

Setback distances between septic tank systems and the shorelines of Lake Okareka, New Zealand were determined from model simulations for a worst-case scenario, using the highest hydraulic conductivity and gradient measured in the field, removal rates of the microbial indicators (Escherichia coli and F-RNA phages) determined from a column experiment, and maximum values of the design criteria for the disposal system, and assuming an absence of an unsaturated zone, a continuous discharge of the raw effluent from a failed or non-complying treatment system (both indicators at concentrations of 1x10(7) counts/100 ml) into the groundwater and no sorption of pathogens in the aquifer. Modelling results suggest that the minimal setback distances were 16 m to satisfy the New Zealand Recreational Water Quality Guidelines for E. coli <126 per 100 ml (Ministry for the Environment, 1999) and 48 m to meet the Drinking-Water Standards for New Zealand 2000 for enteric virus <1 per 100 l (Ministry of Health, 2000). These distances may be applicable for other lakeshores in pumice sand aquifers with groundwater velocities <7 m/day. Findings of laboratory column and batch experiments provided an insight into the microbial attenuation and transport processes in pumice sand aquifers. Bacterial removal was predominately through filtration (87-88%) and partially by die-off (12-13%), while viral removal was by both die-off (45%) and filtration (55%). In addition, microbial die-off in groundwater without aquifer material (i.e., free microbes) was much lower than die-off in groundwater with aquifer material (i.e., sorbed microbes) and contributed only 2-6% to the total removal. This implies that the setback distances estimated from die-off rates for the free microbes, determined in the laboratory without considering aquifer media and other removal processes, which are often reported in the literature, could be larger than necessary.     

Book reviews

Land and Leisure — Concepts and Methods in Outdoor Recreation

Second Edition — edited by C. S. Von Doren, G. B. Priddle and J. E. Lewis.

Methuen & Co., £5.95. 1979.

Countryside Planning — The First Three Decades, 1945–76

Andrew W. Gilg.

Methuen & Co. £3.50. 1979.

Social Problems and the City

D. T. Herbert and D. M. Smith

Oxford University Press. £8.50. 271 pp.

Slum Housing and Residential Renewal — The Case of Urban Britain

D. A. Kirby

Topics in Applied Geography Series. Longmans. £2.95. 1979.

From Blacksmiths to White Elephants: Benwell's Changing Shops

Benwell Community Project Final Report, Series No. 7, Newcastle upon Tyne 1979. 72 pp. 80p.

The Debate on Urban Policy — Decentralisation versus Improvement

Robert Coursey

Retailing and Planning Associates 1977. 93 pp.

Urban Deprivation and Government Initiatives

Paul Lawless

Faber. £8.95 (hard‐back), 251 pp.

A Guide to Historic Buildings Law Fourth Edition

Peace, D., and Purdy, I. M.

Cambridgeshire County Council, County Planning Department, 1979.

98 pp. (paperback), £5.35 post paid.

Research and Intelligence

Papers delivered at the Annual Conference 1978, Inlogov University of Birmingham Edited by E. M. Davies

£4.30. 257 pp.

Geography and the Urban Environment : Progress in Research and Applications

Vol. II. Edited by D. T. Herbert, R. J. Johnston.

Wiley, London. 308 pp.

Facts about the New Towns : A Socio‐Economic Digest

A. G. Champion, K. Clegg and R. L. Davies

R.P.A., Corbridge, 1978.

Physical Planning in the Member States of the E.E.C.

C. Wood and N. Lee, Occasional Paper No. 2.

Department of Town and Country Planning, University of Manchester, 1978. 59pp.     

Book reviews

French Land Use Planning in the Fifth Republic: Real or imagined decentralisation?

Irene B. Wilson, Nijmeegse Planologische Cahiers No. 27, Geografisch en Planologisch Instituut, Katholieke Universiteit Nijmegen, Netherlands, 1988. 228pp. ISBN 90–70219–23–9.

Environmental and Resource Economics: an Introduction

Common, M. (1988). Longman i‐xiv 1–319.

The Greening of Aid: Sustainable Livelihoods in Practice.

C. Conroy & M. Litvinoff (Eds.) Earthscan Publications Ltd, London, 1989, pp.293, £8.95 (pb).

Private Supply of Public Services: Evaluation of Real Estate Exactions, Linkage, and Alternative Land Policies.

Rachelle Alterman (Ed), New York University Press, 1988. 273pp, ISBN 0–8147–0589–8.

City Politics

Hegemonic Projects and Discourse, by Maarten A. Hajer, published by Avebury Press, the Gower Publishing Company Ltd 1989, 140 pp.

The Genius Of The Place

The English Landscape Garden 1620–1820

John Dixon Hunt and Petér Willis. Price £12.50 (Paperback) & £26.95 (Cloth). M.I.T. Press.

Vacationscape

Designing Tourist Regions — 1988 2nd Ed.

Clare A. Gunn, New York, Van Nostrand Reinhold, $34.95 (cloth)

Loft Living — Culture and Capital in Urban Change

Sharon Zukin. Published, British Edition ‘Radius (Century Hudson) London, 1988 Price £8.95.

First Pub. U.S. John Hopkins University Press, 1982. Note: The British Edition has an added Chapter ’More Market Forces’.

William Kent Landscape Garden Designer

John Dixon Hunt. A. Zwemmer Ltd London. 1987, 176pp.

The Pattern of Landscape

Sylvia Crowe and Mary Mitchell (1988). Packard Publishing Ltd, Chichester. 127pp, 161 photographs. Price £30. ISBN 185341 020.

International Environmental Diplomacy

Edited by J. E. Carroll     

Trajectories of change in sagebrush steppe vegetation communities in relation to multiple wildfires

Repeated perturbations, both biotic and abiotic, can lead to fundamental changes in the nature of ecosystems, including changes in state. Sagebrush steppe communities provide important habitat for wildlife and grazing for livestock. Fire is an integral part of these systems, but there is concern that increased ignition frequencies and invasive species are fundamentally altering them. Despite these issues, the majority of studies of fire effects in systems dominated by Artemisia tridentata wyomingensis have focused on the effects of single burns. The Arid Lands Ecology Reserve (ALE), in south-central Washington (U.S.A.), was one of the largest contiguous areas of sagebrush steppe habitat in the state until large wildfires burned the majority of it in 2000 and 2007. We analyzed data from permanent vegetation transects established in 1996 and resampled in 2002 and 2009. Our objective was to describe how the fires, and subsequent postfire restoration efforts, affected communities' successional pathways. Plant communities differed in response to repeated fire and restoration; these differences could largely be ascribed to the functional traits of the dominant species. Low-elevation communities, previously dominated by obligate seeders, moved furthest from their initial composition and were dominated by weedy, early-successional species in 2009. Higher-elevation sites with resprouting shrubs, native bunchgrasses, and few invasive species were generally more resilient to the effects of repeated disturbances. Shrub cover has been almost entirely removed from ALE, although there was some recovery where communities were dominated by resprouters. Bromus tectorum dominance was reduced by herbicide application in areas where it was previously abundant, but it increased significantly in untreated areas. Several resprouting species, notably Phlox longifolia and Poa secunda, expanded remarkably following competitive release from shrub canopies and/or abundant B. tectorum. Our results suggest that community dynamics can be understood through a state and transition model with two axes (shrub/grass and native/invasive abundance), although such models also need to account for differences in plant functional traits and disturbance regimes. We use our results to develop a conceptual model that will be validated with further research.