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851.
Chen G Wu X Zhou WL Li L 《Journal of environmental biology / Academy of Environmental Biology, India》2010,31(5):655-660
C-glucosyltransferase (EC 2.4.1.X) is one of the key enzymes for the biosynthesis of puerarin. This paper describes the methodology in purification and assay of the enzyme for the first time in Puerarin lobata (Wild.) Ohwi. C-glucosyltransferase from roots of P. lobata was extracted and partially purified by (NH4)2SO4 saturation. The effects of pH, temperature, and substrate concentration on the activity of the enzyme were investigated. The properties of the puerarin produced by C-glucosyltransferase were studied by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). The peak activity of C-glucosyltransferase was detected in fraction of by 80% saturation of (NH4)2SO4 and the optimal conditions for enzymatic reaction were 35.5 micromol l(-1) of isoliquiritigenin and 560 micromol l(-1) of UDP-G at pH 8.1, 28 degrees C for 1 h. Mn2+ at 1 mmol l(-1) and Al3+ at 1 mmol l(-1) increased the enzyme activity, while Mg2+ inhibited its activity. The enzyme activity in Nicotiana tabacum and P. lobata were detected under the above assay conditions. Higher activity was found in roots than in leaves and stems of P. lobata, while no enzyme activity was detected in leaves of N. tabacum. It was the first time that activity of C-glucosyltransferase, which transforms isoliquiritigenin to puerarin, was detected in P. lobata. 相似文献
852.
853.
由于缺乏有效监管手段,目前脱硫设施实际投运情况不理想。设计了一套B/S架构的烟气脱硫设施运行及脱硫电价实时监控系统,分为数据采集、数据通讯、数据处理及发布3个子系统。选择能全面反映脱硫设施运行状况的28个信号,采用"共享-分流"信号防伪采集技术进行采集,通过电力系统内载波电话拨号与无线GPRS两种通讯方式相互竞争、互为备用来实现可靠通讯,并经过软件防伪判别模块和统计运算后以WEB形式发布。数据以5m in的间隔实时刷新,且每小时更新脱硫投入率、脱硫电价款等报表数据。 相似文献
854.
855.
Youneng Tang Michal Ziv-El Chen Zhou Jung Hun Shin Chang Hoon Ahn Kerry Meyer Daniel Candelaria David Friese Ryan Overstreet Rick Scott Bruce E. Rittmann 《Frontiers of Environmental Science & Engineering》2010,4(3):280-285
A long-term pilot-scale H2-based membrane biofilm reactor (MBfR) was tested for removal of nitrate from actual groundwater. A key feature of this second-generation pilot MBfR is that it employed lower cost polyester hollow fibers and still achieved high loading rate. The steady-state maximum nitrate surface loading at which the effluent nitrate and nitrite concentrations were below the Maximum Contaminant Level (MCL) was at least 5.9 g·N·(m2·d)?1, which corresponds to a maximum volumetric loading of at least 7.7 kg·N·(m3·d) ?1. The steady-state maximum nitrate surface area loading was higher than the highest nitrate surface loading reported in the first-generation MBfRs using composite fibers (2.6 g·N·(m2·d)?1). This work also evaluated the H2-utilization efficiency in MBfR. The measured H2 supply rate was only slightly higher than the stoichiometric H2-utilization rate. Thus, H2 utilization was controlled by diffusion and was close to 100% efficiency, as long as biofilm accumulated on the polyester-fiber surface and the fibers had no leaks. 相似文献
856.
An integrated process of metal chelate absorption coupled with two stage bio-reduction using immobilized cultures has been proposed to continuously removal of NOx, and the effects of SO2, NO and O2 concentration, gas/liquid flow rate on NOx removal efficiency were investigated. Although nitrogen-containing components, such as Fe(II)EDTA-NO, NO2? and NO3? in the scrubbing solution, inhibited the bio-reduction of Fe(III)EDTA obviously, it was feasible to abate the inhibition effect by using the two stage bio-reduction system, and thus to improve NOx removal efficiency. The removal efficiency decreased slowly with the increase of SO2, O2, NO concentration and gas flow rate, and increased with the increase of liquid flow rate. Continuously operating for 18 days, a high removal efficiency around 95% was reached by using the two-stage bio-reduction system with immobilized microorganisms, while the value decreased to 85% after 5 days of operation by using the suspended microorganisms, at a constant gas flow rate of 60 L/h containing 424–450 mg/m3 NO, 2428–2532 mg/m3 SO2 and 3% O2. 相似文献
857.
858.
本文对发改委颁布的(《温室气体自愿减排交易管理暂行办法》进行了解读、分析其特点并指出尚需进一步解决的问题。 相似文献
859.
860.