A three-isotope approach to disentangling the diet of a generalist consumer: the yellow-legged gull in northwest Spain

The widespread omnivory of consumers and the trophic complexity of marine ecosystems make it difficult to infer the feeding ecology of species. The use of stable isotopic analysis plays a crucial role in elucidating trophic interactions. Here we analysed δ¹⁵N, δ¹³C and δ³⁴S in chick feathers, and we used a Bayesian triple-isotope mixing model to reconstruct the diet of a generalist predator, the yellow-legged gull (Larus michahellis) that breeds in the coastal upwelling area off northwest mainland Spain. The mixing model indicated that although chicks from all colonies were fed with a high percentage of fish, there are geographical differences in their diets. While chicks from northern colonies consume higher percentages of earthworms, refuse constitutes a more important source in the diet of chicks from western colonies. The three-isotope mixing model revealed a heterogeneity in foraging habitats that would not have been apparent if only two stable isotopes had been analysed. Moreover, our work highlights the potential of adding δ³⁴S for distinguishing not only between terrestrial and marine prey, but also between different marine species such as fish, crabs and mussels.     

Short- and long-term consequences of prenatal testosterone for immune function: an experimental study in the zebra finch

Hormone-mediated maternal effects play an important role in the formation of a differentiated phenotype. They have been shown to influence a wide array of offspring traits, both early in life and in adulthood. One important offspring trait that is under the influence of maternal androgens is the immune system. In birds, a growing number of studies show that yolk androgens modulate immune function during the chick stage. However, there is a lack of knowledge regarding long-term effects of prenatal androgens on offspring immunity. In this study, we therefore investigated the influence of prenatal testosterone (T) on several measures of immunity in fledgling and adult zebra finches (Taeniopygia guttata). Cell-mediated immune response (towards phytohaemagglutinin, PHA) of fledglings hatching from control eggs was negatively related to brood size, whereas there was no such association for fledglings hatching from eggs with experimentally elevated T levels (T fledglings). Male control fledglings showed reduced mass gain compared to female control fledglings within 24 h after the PHA injection. This pattern was reversed in T fledglings. Total antibody levels in fledglings were not affected by egg treatment. Neither cell-mediated immunity nor total antibody levels in sexually mature zebra finches were influenced by egg treatment. However, there was an immuno-enhancing effect of elevated egg T on both primary and secondary humoral immune responses toward diphtheria and tetanus antigens in ca 5 and 7 month old zebra finches. In addition, the covariation between different immune components differed between T and control offspring, suggesting that egg treatment may have altered the potential trade-offs between different parts of the immune system. Our results suggest that prenatal androgens could be an important factor contributing to individual variation in immune function even in adulthood.     

Habituation to low-risk predators improves body condition in lizards

Habituation to nonlethal predation stimuli may provide benefits for animals living in areas with frequent encounters with low-risk predators. On the other hand, individuals can be very consistent in their antipredator responses, with shy individuals showing greater degree of responsiveness than bold individuals. However, the link between habituation or boldness and individual benefits has not been thoroughly investigated. We established whether and how two behavioral components associated with antipredator responses (habituation and boldness, and their interaction) would influence body condition, which is a parameter related to fitness. We conducted an outdoor semi-natural experiment with Iberian wall lizards (Podarcis hispanica). Individual boldness was consistent across contexts, but we did not find any effect of boldness or the interaction between boldness and habituation on body condition. However, those individuals that habituated more readily to a frequent predatory stimulus were able to increase their body condition more relative to lizards that habituated less. This finding highlights the importance of individual differences in behavioral plasticity, which could influence traits related to fitness. Habituation can provide benefits for individuals exposed to low-risk predators; however, individuals more prone to habituation could also experience mortality costs by wrongly habituating to a dangerous predator.     

Correlates of male mating success in great bustard leks: the effects of age, weight, and display effort

We examined how mating success varied in relation to age, weight, body size, and display behavior among great bustard Otis tarda males. The estimated mating success was strongly skewed, with 45% of adult males being involved in copulation attempts and only 9.7% actually seen copulating successfully. Unlike most birds, body size continued increasing in great bustards several years after reaching sexual maturity. Age, weight, and display effort were all significant and independent predictors of male mating success. The higher display effort involved performing longer full-display bouts. Older males could detach from the male flock earlier in the season as well as on each day and spend longer seasonal and daily periods displaying as solitary birds, which contributed to increase their mating success. In contrast, males weighing more did not invest more in display, which suggests that they could be recognized as dominants by other males and selected by females through assessment of their plumage sexual traits. In contrast to most other bird species, the system described for great bustards resembles that found in some lek-mating ungulates, where social rank is a complex trait determined by both age and mass, and as in these mammals, it suggests that sexual selection continues to favor a high male weight in this extremely sexually dimorphic species.     

Spatial extremes of wildfire sizes: Bayesian hierarchical models for extremes

In Portugal, due to the combination of climatological and ecological factors, large wildfires are a constant threat and due to their economic impact, a big policy issue. In order to organize efficient fire fighting capacity and resource management, correct quantification of the risk of large wildfires are needed. In this paper, we quantify the regional risk of large wildfire sizes, by fitting a Generalized Pareto distribution to excesses over a suitably chosen high threshold. Spatio-temporal variations are introduced into the model through model parameters with suitably chosen link functions. The inference on these models are carried using Bayesian Hierarchical Models and Markov chain Monte Carlo methods.     

An indexed dispersion criterion for testing the sex-biased dispersal of lek mating behavior of capercaillies

Traditionally it has been assumed by ecologists that the dispersion of positions with respect to leks of female capercaillies during the mating season is superior to the dispersion of positions of male capercaillies. However some recently published articles suggest the idea that dispersion is not sex-biased, but both sexes show a similar dispersion. This article introduces a mathematical model to approach the study of the above question by means of an indexed dispersion criterion. This model is developed in detail thus deriving its main mathematical properties. On this basis, the model is applied to the analysis of the motivating problem with real data of positions of leks and female and male capercaillies. The results derived by means of the model put into doubt the traditional assumption of a superior dispersion of females supporting the new theories, that is, both sexes show a similar dispersion.     

Screening of phthalic acid esters in raw materials,premixes and feed additives

Phthalates are animal carcinogens and may cause death or tissue deformities. Samples of feedstuffs collected in 2005 and 2006 from industrial feed manufacturers in the Czech Republic were analysed for contamination with phthalic acid esters (PAEs), specifically di-2-ethylhexyl phthalate (DEHP) and di-n-butyl phthalate (DBP). Samples of feed additives, premixes and raw materials were collected (year 2005, n = 26). For soybean oil, the total volume of phthalates measured (DBP + DEHP) reached a level of 131.42 mg kg⁻¹; for rapeseed oil, fish meal and animal fats, the levels measured were 15.00, 7.96 and 58.87 mg kg⁻¹, respectively. The lowest level of DBP + DEHP was found in corn (2.03 mg kg⁻¹). Since phthalates were detected, samples of feed additives (n = 28) and raw materials (n = 28) were collected again in 2006. The highest levels of DBP + DEHP were found in raw materials containing fat. Phthalate levels in rapeseed oil samples ranged from 1.38 to 32.40 mg kg⁻¹ DBP + DEHP. For feed additives, contamination levels in vitamins and amino acids ranged from 0.06 to 3.15 and 1.76 to 4.52 mg kg⁻¹ DBP + DEHP, respectively. Here, we show that the levels of PAEs found in cereals such as wheat, barley and corn may be regarded as being alarmingly high, because cereals make up the largest proportion of compound feed of farm animals.     

Recycling glass-polishing sludge and aluminum anodising sludge in polyurethane and cement composites: fire performance and mechanical properties

Journal of Material Cycles and Waste Management - This article aims to study the mechanical strength and fire resistance of polyurethane/cement (PuCem) composites containing glass sludge and sludge...     

Bio-methane production from tomato pomace: preliminary evaluation of process intensification through ultrasound pre-treatment

Journal of Material Cycles and Waste Management - The effect of ultrasound pre-treatment (for 5, 15, and 30 min) on tomato pomace methane yield was evaluated. With respect to the control...     

The Impact of Biodegradable Plastics in the Properties of Recycled Polyethylene Terephthalate

Journal of Polymers and the Environment - Since biodegradable materials are unwittingly mixed with synthetic materials, this work aimed to study the feasibility of reliably identifying some...