Three Different Topics for Bioremediation Application on Soils and Waters Contaminated with Aromatic Compounds, by Using Immobilized Bacteria

Molecular techniques and modelling are presented as powerful tools required in the performance of efficient soil and water bioremediation systems. An Escherichia coli CC118-D strain was constructed by inserting the Klebsiella pneumoniae hpa B gene, coding for the unstable 3,4-dihydroxyphenylacetate 2,3-dioxygenase, into its chromosome. When the constructed strain was immobilized, both enzyme stability and viability increased along the studied period, in absence of antibiotic. We proposed this strategy as an approach to overcoming plasmid instability and to enhance enzyme activity and stability, avoiding antibiotic utilization. A model was developed to understand and predict the behaviour of bacteria and pollutants in a bioreactor system, considering: fluid dynamics, molecular/cellular scale processes and biofilm formation.     

Biodepressive effect of zinc on humoral effector of theHolothuria polii immune response

High zinc ion concentrations produce an inhibitory effect on the naturally occurring hemolytic activity of theHolothuria polii coelomic fluid and coelomocyte lysate. The naturally occurring hemagglutinating activity remains unaffected. The inhibition appears reversible, suggesting a non-covalent interaction of the ions with the hemolysins. Moreover, as demonstrated by SDS-PAGE and immunoblotting analysis, the Zn⁺⁺ treatment does not produce molecular weight variations in the coelomic fluid and coelomocyte lysate hemolytic patterns. This study was conducted during 1987 on samples gathered from the Gulf of Palermo, Italy.     

Natural radiation exposure in the Campo Arañuelo region in the surroundings of Almaraz nuclear power station (Spain)

During 1998 regional surveys were conducted to evaluate natural radiation exposure of people in the vicinity of the six Spanish nuclear power stations. Indoor radon, external gamma dose rates outdoors and indoors and radioactivity in soil were measured in these surveys. The highest mean annual effective dose to the population was found in the surroundings of the Almaraz nuclear power plant in the province of Cáceres and was mainly due to the presence of high radon concentrations in homes. In order to make a more accurate assessment of the dose coming from the radon in this area, a new and more extensive surgery on indoor radon with a total of 380 measurements was carried out in 2000 in the Campo Ara?uelo region around the Almaraz nuclear power station. From the results obtained in this survey, a population-weighted mean annual effective dose from radon exposure of 1.7 mSv y(-1), 44% lower than that previously reported in 1998, was estimated for the whole Campo Ara?uelo region. The maximum dose value due to radon exposure, about 4 mSv y(-1), was reached in the so-called La Vera area located in the north of this region. The relationship between the indoor radon concentrates experimentally measured, the geological characteristics of this geographic area and the information provided by the radiation map of the Spanish MARNA Project is also analyzed, confirming that La Vera area should be considered as a high radon level area.     

Anaerobic digestion of linear alkyl benzene sulfonates: biodegradation kinetics and metabolite analysis

In the present work the effect of the alkyl chain length and the position of the sulfophenyl substituent of the linear alkylbenzene sulfonates (LAS) on their anaerobic biodegradability have been investigated. Degradation kinetics of the linear alkyl benzene sulfonates homologues, 2C₁₀LAS, 2C₁₂LAS and 2C₁₄LAS, have been studied. It has been also investigated the effect of the isomer type on the degradation rate of the LAS molecule through the comparative study of the 2C₁₀LAS and 5C₁₀LAS isomers. Batch anaerobic biodegradation tests were performed using sludge from the anaerobic digester of a wastewater treatment plant as microorganisms source. Ultimate biodegradation was evaluated from the biogas production whereas primary biodegradation was determined by specific analysis of the surfactant. LAS homologues and isomers showed a negligible primary biodegradation under anaerobic conditions. Furthermore, analysis of sulfophenyl carboxilates (SPC) by LC–MS indicated a low and constant level of these LAS degradation metabolites over the test period. These data are consistent with a minimal transformation of the LAS parent molecule in the anaerobic digesters. On the other hand, the addition of the shortest alkyl chain length homologues, decyl and dodecylbenzene sulfonates, reduces the biogas production whereas the most hydrophobic homologue, the tetradecylbenzene sulfonate, enhances the biogas production. This LAS homologue seems to increase the availability of organic compounds sorbed on the anaerobic sludge promoting their biodegradation.     

Mercury accumulation in soils and plants in the Almadén mining district, Spain: one of the most contaminated sites on Earth

Although mercury (Hg) mining in the Almadén district ceased in May 2002, the consequences of 2000 years of mining in the district has resulted in the dissemination of Hg into the surrounding environment where it poses an evident risk to biota and human health. This risk needs to be properly evaluated. The uptake of Hg has been found to be plant-specific. To establish the different manners in which plants absorb Hg, we carried out a survey of Hg levels in the soils and plants in the most representative habitats of this Mediterranean area and found that the Hg concentrations varied greatly and were dependent on the sample being tested (0.13–2,695 μg g⁻¹ Hg). For example, the root samples had concentrations ranging from 0.06 (Oenanthe crocata, Rumex induratus) to 1095 (Polypogon monspeliensis) μg g⁻¹ Hg, while in the leaf samples, the range was from 0.16 (Cyperus longus) to 1278 (Polypogon monspeliensis) μg g⁻¹ Hg. There are four well-differentiated patterns of Hg uptake: (1) the rate of uptake is constant, independent of Hg concentration in the soil (e.g., Pistacia lentiscus, Quercus rotundifolia); (2) after an initial linear relationship between uptake and soil concentration, no further increase in Hg_plant is observed (e.g., Asparagus acutifolius, Cistus ladanifer); (3) no increase in uptake is recorded until a threshold is surpassed, and thereafter a linear relationship between Hg_plant and Hg_soil is established (e.g., Rumex bucephalophorus, Cistus crispus); (4) there is no relationship between Hg_plant and Hg_soil (e.g., Oenanthe crocata and Cistus monspeliensis). Overall, the Hg concentrations found in plants from the Almadén district clearly reflect the importance of contamination processes throughout the study region.     

Simulation of germination of pioneer species along an experimental drought gradient

The germination of ten plant species from the Iberian Peninsula was assessed along a water deficit gradient between -0. 1652 (moist) and -0.4988 MPa (dry) of osmotic potential, created by addition of increasing concentrations of polyethylene glycol (PEG 6000) to distilled water in which plants were grown hydroponically. The level and rate of germination of Daucus carota and Thapsia villosa significantly decreased with decreasing psi. Seeds of Dactylis glomerata and Dittrichia viscosa had positive germination responses to low osmotic potentials; germination of Epilobium hirsutum was not affected by osmotic potential. Germination of Medicago arabica, Cynosurus cristatus, Cistus ladanifer and Cistus albidus, was no favored by the addition of polyethylene glycol (PEG). Germination of Foeniculum vulgare and Thapsia villosa was inhibited by PEG.     

Binding of Pb(II) in the system humic acid/goethite at acidic pH

The study of Pb(II) binding to the system humic acid/goethite in acidic medium is reported in the present paper. From a macroscopic point of view, we have constructed the experimental sorption isotherms (using atomic absorption spectroscopy) and compared them with the prediction of the additivity rule. It is found that this system presents positive deviations, that is, the amount sorbed is about an order of magnitude higher than predicted. Apart from this, microscopic and structural aspects have also been studied using in situ and ex situ infrared spectroscopy. These results suggest that the presence of Pb(II) increases the amount of humic acid bound to the oxide. It is proposed that proton displacement due to the interaction between humic substances and the oxide, along with the formation of ternary complexes with the Pb(II) cation bridging the oxide and the humic substances (Type A complexes), cause the deviation from additivity.