Evaluating the importance of demographic connectivity in a marine metapopulation

Recently researchers have gone to great lengths to measure marine metapopulation connectivity via tagging, genetic, and trace-elemental fingerprinting studies. These empirical estimates of larval dispersal are key to assessing the significance of metapopulation connectivity within a demographic context, but the life-history data required to do this are rarely available. To evaluate the demographic consequences of connectivity we constructed seasonal, size-structured metapopulation matrix models for two species of mytilid mussel in San Diego County, California, USA. The self-recruitment and larval exchange terms were produced from a time series of realized connectivities derived from trace-elemental fingerprinting of larval shells during spring and fall from 2003 to 2008. Both species exhibited a strong seasonal pattern of southward movement of recruits in spring and northward movement in fall. Growth and mortality terms were estimated using mark-recapture data from representative sites for each species and subpopulation, and literature estimates of juvenile mortality. Fecundity terms were estimated using county-wide settlement data from 2006-2008; these data reveal peak reproduction and recruitment in fall for Mytilus californianus, and spring for M. galloprovincialis. Elasticity and life-stage simulation analyses were employed to identify the season- and subpopulation-specific vital rates and connectivity terms to which the metapopulation growth rate (lambda) was most sensitive. For both species, metapopulation growth was most sensitive to proportional changes in adult fecundity, survival and growth of juvenile stages, and population connectivity, in order of importance, but relatively insensitive to adult growth or survival. The metapopulation concept was deemed appropriate for both Mytilus species as exchange between the subpopulations was necessary for subpopulation persistence. However, highest metapopulation growth occurred in years when a greater proportion of recruits was retained within the predominant source subpopulation. Despite differences in habitat and planktonic duration, both species exhibited similar overall metapopulation dynamics with respect to key life stages and processes. However, different peak reproductive periods in an environment of seasonal current reversals led to different regional (subpopulation) contributions to metapopulation maintenance; this result emphasizes the importance of connectivity analysis for spatial management of coastal resources.     

DNA-based carrier detection and prenatal diagnosis of tyrosinase-negative oculocutaneous albinism (OCA1A)

We describe molecular prenatal diagnosis and carrier detection of tyrosinase-negative oculocutaneous albinism (OCA1A) in two families. In one family, we carried out DNA-based prenatal diagnosis of OCA1A. In the other family, mutation analysis and carrier detection obviated the need for prenatal diagnosis. Molecular analysis is safer and probably more accurate than fetoscopy and fetal scalp biopsy, and should become the method of first choice for prenatal diagnosis of OCA1.     

Sediment and nutrient dynamics during snowmelt runoff generation in a southern Taiga catchment of Russia

The water quality of the Volga catchment, Russia, is diminished and major nutrients reach critical levels. Proper management strategies depend on process knowledge about nutrient sources and pathways as well as appropriate models. For the 18.8 km² experimental catchment Lubazhinkha, 100 km south of Moscow, a monitoring scheme was conducted to identify runoff generation and nutrient source areas. The grey forest soils of the catchment are utilized with arable land and pasture (54%) and forest areas. The received results show a high intra-annual and inter-annual dynamic of the snowmelt runoff generation as major hydrological process. Overall sediment export rates during the 3 years of snowmelt investigation from 2003 to 2005 are low with a maximum of 0.33 t ha⁻¹ during snowmelt period 2005 and effectively reduced by a dam at the catchment outlet. Also, the total phosphorus loss from the catchment is low reaching from 0.03 to 0.35 kg ha⁻¹ during the snowmelt periods. However, sediment and total phosphorus concentrations vary within one snowmelt event and can reach high concentrations. Detailed analysis of element concentrations during snowmelt events allows an identification of different flow components and runoff generating mechanisms. During low flow situations phosphorus and dissolved organic carbon are transferred from the arable soils with slow flow components to the channel. In contrast to this situation, the sources of sediment and nutrients split up temporarily and spatially during high flow situations. Aside arable fields that are source of sediment and phosphorus during snowmelt episodes, the forested areas were detected as major source for the export of dissolved organic carbon with surface runoff. In particular these areas are characterized by a late thawing of the topsoil which causes a delayed peak of dissolved organic carbon concentration. The awareness of the dynamic and complex catchment reaction during snowmelt events is of importance for the development of proper management strategies.     

Fine-scale spatial patterns of the Tertiary relict Zelkova abelicea (Ulmaceae) indicate possible processes contributing to its persistence to climate changes

In this paper, the fine-scale spatial patterns of the Tertiary relict Zelkova abelicea (Lam.) Boiss. were studied (1) to reveal processes that contributed to its persistence to climate changes and (2) to assist future conservation planning, with the purpose of shifting the attention of conservation practitioners from patterns to processes. Results of the fine-scale spatial patterns of Z. abelicea indicate that the species tolerates disturbance and/or tracks changes resulting from disturbance in the range of its distribution through morphological and reproductive plasticity. In addition, our study indicates that Z. abelicea populations are conserved in the absence of metapopulation structure and that the species participates in plant–plant interactions through facilitation processes. Hence, the persistence of the species to climate changes seems to be more complicated and multifactorial than a linear and plain view of species survival in climate refugial areas, and therefore calls for a consideration of the processes revealed in this paper in future conservation planning.     

Influence of air humidity on sampling efficiency of some solid adsorbents used for sampling organics from work-room air

The influence of air humidity on the sampling efficiency of Amberlite XAD porous polymers and activated charcoal was studied by determining the recovery of various organics at 20% and 85% relative humidity. The sampling efficiency of XAD-7 was found to decrease with increasing relative humidity, while the sampling efficiency of XAD-2 and activated charcoal was relatively unaffected for the compounds studied. Activated charcoal had a greater capacity than Amberlite XAD for several types of compounds, however.     

Records of atmospheric delivery of pyrolysis-derived pollutants in recent mountain lake sediments of the Julian Alps (NW Slovenia)

The historical record of the input of pyrolysis-derived pollutants via the atmosphere, i.e. black carbon (BC), polycyclic aromatic hydrocarbons (PAH) and spheroidal carbonaceous particles (SCP) was measured in the sediments of three remote alpine lakes situated in the Julian Alps, northwest Slovenia. Parallel sedimentary trends for BC, PAH and SCP were observed across the Julian Alps and relationships among these contaminants are discussed. Inputs of these pyrolytic-contaminants to sediments of remote lakes started to rise at the end of the 19th century, while their peak inputs were observed in the mid-20th century, amounting up to 6.0 gm(-2) yr(-1) for BC, 5200 x 10(4) m(-2) yr(-1) for SCP and 2900 microg m(-2) yr(-1) for PAH. In the last two to three decades, inputs decreased substantially, by a factor of up to 3 for BC, 7 for SCP and at least 3 for PAH.     

Performance of the Button Personal Inhalable Sampler for the measurement of outdoor aeroallergens

No personal aerosol sampler has been evaluated for monitoring aeroallergens in outdoor field conditions and compared to conventional stationary aerobiological samplers. Recently developed Button Personal Inhalable Aerosol Sampler has demonstrated high sampling efficiency for non-biological particles and low sensitivity to the wind direction and velocity. The aim of the present study was to evaluate the Button Sampler for the measurement of outdoor pollen grains and fungal spores side-by-side with the widely used Rotorod Sampler. The sampling was performed for 8 months (spring, summer and fall) at a monitoring station on the roof of a two-storied office building located in the center of the city of Cincinnati. Two identical Button Samplers, one oriented towards the most prevalent wind and the other towards the opposite wind and a Rotorod Sampler were placed side-by-side. The total fungal spore concentration ranged from 129 to 12,980 spores m(-3) (number per cubic meter of air) and the total pollen concentration from 4 to 4536 pollen m(-3). The fungal spore concentrations obtained with the two Button Samplers correlated well (r = 0.95; p<0.0001). The pollen data also showed positive correlation. These findings strongly support the results of earlier studies conducted with non-biological aerosol particles, which demonstrated a low wind dependence of the performance of the Button Sampler compared to other samplers. The Button Sampler's inlet efficiency was found to be more dependent on wind direction when sampling larger sized Pinaceae pollen grains (aerodynamic diameter approximately 65 mum). Compared to Rotorod, both Button Samplers measured significantly higher total fungal spore concentrations. For total pollen count, the Button Sampler facing the prevalent wind showed concentrations levels comparable to that of the Rotorod, but the Button Sampler oriented opposite to the prevalent wind demonstrated lower concentration levels. Overall, it was concluded that the Button Sampler is efficient for the personal sampling of outdoor aeroallergens, and is especially beneficial for aeroallergens of small particle size.