Mesoderm induction by transforming growth factor β: Medium conditioned by TGF-β-treated ectoderm enhances the inducing activity

Dedicated to Professor Dr. Tuneo Yamada on the occasion of his 80th birthday.     

In vitro characteristics of human fetal cells obtained from chorionic villus sampling and amniocentesis

In vitro characteristics of human fetal cells have been investigated after chorionic villus sampling at the first trimester and amniocentesis at the second trimester of pregnancy. Light microscopy revealed heterogeneous morphology of cell types in both the chorionic villus culture and the amniotic fluid cultures. Based on the experiments performed, chorionic villus cells are more sensitive to pronase, trypsin, and versene during subculture and have a higher DNA content per single cell and release more [¹²⁵I]-Beta-human chorionic gonadotropin into culture medium than those found in amniotic fluid cells. The practical applications of this study are discussed.     

美国化学品危害信息的传递

1970年美国颁布<职业安全卫生法>,要求企业将作业场所化学品的危害告知作业人员.为贯彻该法的要求,20世纪80年代初期,美国职业安全卫生管理局(OSHA)颁布危害信息传递标准(HCS),要求化学品生产和进口商对生产和进口的化学品进行危害评估,并要求所有企业将有害化学品的信息传递给作业人员.     

厦门西港海水质量对鱼卵胚胎发育畸形率的影响

以养殖场培育的花尾胡椒鲷受精卵为实验材料测试了厦门西域5个站位的表层水水样对花尾胡椒鲷受精卵的胚胎发育毒性，并运用GC－MS手段分析了水样中的美国EPA优控的16PAHs组分的含量。结果表明：3号站位（厦门市工业和生活污水主要排放口的外侧）和4号站位（厦门西港的港区）的水样对鱼卵的胚胎发育的毒性最强。8号站位（位于主航道并濒临嵩屿电厂）的水样对鱼卵胚胎发良的毒性次之，1号站位（湾口）和5号站位（内港）的水样对鱼卵的胚胎发育的毒性较小。水样中的PAHs含量分析结果表明，仅用水样中的PAHs含量不能全面地反映水样对鱼卵胚胎发育的毒性。最后，对本实验方法应用于海洋环境生物监测的有效性进行了探讨。