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121.
Iron-stress-mediated effects on biochemical constituents of the red tide dinoflagellateGymnodinium sanguineum Hirasaka were examined in 1988 by comparing Fe-replete and Fe-deplete batch cultures. The influence of nitrogen source (NO3 or NH4) on characteristics of Fe-deplete cells was also studied [i.e., Fe-deplete/NO3-grown (= — Fe/NO3) vs Fe-deplete/NH4-grown (= — Fe/NH4)]. Common to both N sources were reductions of chlorophylla (chla) and Fe quotas (per cell volume) by 75% and ca. 1.5 orders of magnitude, respectively, under Fe depletion. The Fe requirement ofG. sanguineum exceeded those of certain neritic diatoms by one to two orders of magnitude. — Fe/NH4 cells exhibited 30 to 50% greater N quotas and free amino acid:protein ratios than did Fe-deplete cells grown on NO3. In vivo fluorescence:chla increased with Fe deficiency particularly in — Fe/NO3 cultures, surpassing — Fe/NH4 values by ca. two-fold. Effects of Fe depletion were consistent with this element's essential role in the biosynthesis of chla and components of the photosynthetic electron transport (PET) system, and also in NO3 utilization. Fe:N ratios were larger (1.5-fold) for iron-deficient NO3-grown than NH4-grown cells, likely reflecting the Fe content of NO3 assimilatory enzymes [nitrate (NR) and nitrite (NiR) reductase] and of electron transport components needed to provide reductant, coupled with a diminished capacity of — Fe/NO3 cells to acquire and assimilate nitrogen. Indicators of PET efficiency suggested that under iron stress, supply of Fe for NR and NiR is partly at the expense of iron-containing PET components. Utilization of nitrate by NO3-grown cells was inhibited sufficiently by Fe depletion to yield symptoms bordering on N deficiency. In an ecological context, the most important effect mediated by nitrogen source may be the determination of critical QFe (i.e., Fe required to just sustain maximal growth), thereby regulating the degree of growth limitation for a given subsaturating iron concentration.  相似文献   
122.
Porphyra perforata J. Ag. was collected from a rocky land-fill site near Kitsilano Beach, Vancouver, British Columbia, Canada and was grown for 4 d in media with one of the following forms of inorganic nitrogen: NO 3 - , NH 4 + and NO 3 - plus NH 4 + and for 10 d in nitrogen-free media. Internal nitrogen accumulation (nitrate, ammonium, amino acids and soluble protein), nitrate and ammonium uptake rates, and nitrate reductase activity were measured daily. Short initial periods (10 to 20 min) of rapid ammonium uptake were common in nitrogen-deficient plants. In the case of nitrate uptake, initial uptake rates were low, increasing after 10 to 20 min. Ammonium inhibited nitrate uptake for only the first 10 to 20 min and then nitrate uptake rates were independent of ammonium concentration. Nitrogen starvation for 8 d overcame this initial suppression of nitrate uptake by ammonium. Nitrogen starvation also resulted in a decrease in soluble internal nitrate content and a transient increase in nitrate reductase activity. Little or no decrease was observed in internal ammonium, total amino acids and soluble protein. The cultures grown on nitrate only, maintained high ammonium uptake rates also. The rate of nitrate reduction may have limited the supply of nitrogen available for further assimilation. Internal nitrate concentrations were inversely correlated with nitrate uptake rates. Except for ammonium-grown cultures, internal total amino acids and soluble protein showed no correlation with uptake rates. Both internal pool concentrations and enzyme activities are required to interpret changes in uptake rate during growth.  相似文献   
123.
Nitrogen cycling in a natural planktonic food chain was followed before and after perturbation by copper (10 g l-1). Observations were separated into effects from artificial containment of the food chain and effects from the addition of copper. An initial reduction of seston-N and subsequent recovery, uniformly high settling rates of suspended particulate-N, and general decrease in zooplankton-N were attributed to the physical limitations imposed by the enclosures (e.g. elimination of normal mixing processes). Acute inhibition of nitrate assimilation by the seston, acute elevation of seston-N settling and chronic inhibition of zooplankton feeding, along with accelerated decline in zooplankton stocks, were attributed to copper stress. Since zooplankton stocks and fluxes were most drastically affected by copper, its overall effect on N-cycling through the planktonic food chain would depend largely upon the importance of zooplankton in regulating the production of the remaining seston.  相似文献   
124.
Chemical interactions among marine microalgae were studied in cultures of pennate diatoms on agar plates. Nine marine and freshwater pennate diatoms were surveyed as potential bioassay organisms; Cylindrotheca fusiformis was most favourable for assays because of its rapid and even growth on agar. Diatom and bacterial bioassays were used to screen cell and filtrate extracts of 14 microalgal cultures. A number of these algal species, which were grown axenically, produced extractable, intracellular and/or extracellular substances that inhibited the growth of C. fusiformis. Our results suggest that the culturing of pennate diatoms on a solid medium can provide a simple bioassay for screening algal extracts which potentially contain growth inhibitors involved in microalgal allelopathy.  相似文献   
125.
Policies designed to conserve sites of nature conservation importance are an important aspect of city planning in the UK. London has led the way in putting in place a spatial hierarchy of sites of nature conservation importance designed to protect wildlife habitats from development. Some wasteland habitats associated with derelict and vacant land receive protection in this way but development pressure on these so-called 'brownfield sites' is high and is likely to continue. This paper examines how conservation professionals in the private, public and voluntary sectors are responding to the threats of biodiversity loss and opportunities for habitat creation posed by re-development of brownfield sites. The study draws on in-depth interviews conducted with conservation professionals and the practices employed by ecological advisers employed by developers seeking to re-develop wasteland sites. It finds that practitioners are negotiating their role in the re-development process in different ways. Key issues relate to the role of ecological science in codifying wasteland habitats, uncertainties about how best to evaluate the conservation importance of such sites and the strategies and tactics employed by different practitioners as they seek to mobilise a range of knowledges and practices to secure ecologically sensitive proposals. Scientific knowledge about wasteland habitats has not stabilised in ways that can consistently inform conservation policy and practice. As a result biodiversity issues of wasteland sites are often discounted in the re-development process. Investment in studies of the basic ecology of urban wastelands could provide a firmer scientific foundation on which conservation policies could build. At the same time, many conservation professionals involved in urban re-development are struggling to promote a pro-active approach to secure environmentally sensitive development. The knowledge and co-operation networks being mobilised to support this approach are fluid and unstable, and favourable development outcomes as yet are few. Formalising these networks to achieve more effective engagements with developers and the construction industry could consolidate ecological practices designed to conserve and re-create the biodiversity of wasteland habitats.  相似文献   
126.
Too often in the past, public relations professionals were consulted only when there was a problem—or a crisis—that needed explaining. The author provides a model for a process-oriented approach to environmental communication that focuses on building and sustaining positive public relationships over time. The key is dialogue with critical publics as well as links with other important actors in the environmental drama that can help position you as part of the solution, not the problem.  相似文献   
127.
Light regime, riboflavin, and pH effects on 2,4-D photodegradation in water   总被引:1,自引:0,他引:1  
A laboratory study was conducted to determine the effects of light regime, riboflavin, and pH on photodegradation of 2,4-D in aqueous solution. In controlled-environment chamber experiments, riboflavin sensitized 2,4-D photolysis in a concentration-dependent manner under both attenuated UV (-UV) and enhanced UV (+UV) light regimes. The photolysis half-life of 2,4-D in solutions containing 10 mg L-1 riboflavin was 9.7 and 12.5 h when exposed to +UV and -UV, respectively, compared to no photolysis in the absence of riboflavin. In contrast, the extrapolated half-life of 2,4-D in solutions containing 2.5 mg L-1 riboflavin was 46 h under +UV and 72 h under -UV. The rate of 2,4-D photolysis in the presence of riboflavin increased under both light regimes as initial pH of the solution was decreased from 7.5 to 4.5. The half-life of 2,4-D in the presence of 10 mg L-1 riboflavin at pH 4.5 and exposed to +UV was 1.6 h. Lumichrome, a principal photoproduct of riboflavin, did not photosensitize 2,4-D. Concentrations of 2,4-dichlorophenol formed as a result of riboflavin-sensitized 2,4-D photolysis were higher under the -UV than the +UV regime. These results indicate that riboflavin concentration, solution pH, and light regime are interacting factors that may be manipulated to enhance rates of aqueous 2,4-D photolysis.  相似文献   
128.
Tracer exchange kinetics were consistent with the view that microplankton of waters near Hawaii produced and assimilated dissolved orthophosphate and dissolved compounds other than orthophosphate at roughly equal rates in experiments conducted in September, 1982. Using simple exchange models plus chemical measurements, we inferred that material other than orthophosphate could sometimes dominate the soluble molybdate-reactive pool (SRP), as is frequently the case in fresh waters. Where phosphorus exchange was most rapid, the tracer kinetics suggested that some fraction of the plankton community was disproportionately responsible for dissolved phophorus exchange. Comparisons against independent measurements of rapid phytoplankton turnover in these communities supported the conclusion that dissolved compounds other than orthophosphate were rapidly cycled and formed an important substrate for plankton growth.  相似文献   
129.
ABSTRACT: Maintenance of the more than 24 million septic tanks in the U.S. requires removal and disposal of septage. Disposal options include application to agricultural lands where the nutrients and organic matter can provide soil benefits. However, pathogens and contaminants are also contained in septage. An extensive search turned up very few data on septage quality, and those reveal high variability. The data used by the U.S. Environmental Protection Agency (EPA) in developing regulations had the lowest metal concentrations among the nine data sets that could be compared. Based on these data, EPA assumed that septage could be applied to agricultural land for more than 100 applications before reaching unacceptable cumulative loading of metals. They thus did not establish federal standards for metals in septage, and no monitoring is required under federal rules governing septage disposal. Analysis of the nine data sets we found showed that field site life would be reached in less than 100 applications for most septage and cumulative loading limits established by EPA for sewage sludges will be exceeded in 16 applications for some septage as opposed to the 100 application estimate used by EPA. Determination of acceptable cumulative loading depends on numerous technical and policy considerations. All septage sources reached the more restrictive loading limits such as those established by the New York State Department of Environmental Conservation (NYSDEC) and the recommendations in Cornell publications in less than 100 applications. In one case the cumulative limit for copper was exceeded in a single application. These findings suggest additional data are needed on septage quality and that the federal and state agencies responsible for regulating application of septage to agricultural land should reassess their standards.  相似文献   
130.
Work-related traumatic forestry and sawmill fatalities were studied as part of a larger study of all work-related traumatic fatalities in Australia from 1982 to 1984. Data on 79 cases were obtained from inspection of coroners' files. The fatality incidence per 100,000 person-years was extremely high for loggers (396), and high for forest service workers (52) and workers employed in sawmills (30), compared to the entire Australian workforce (8.1). Felling/trimming trees, traffic-related activities and operating heavy machinery (forestry workers) and loading/unloading logs and operating bench saws (sawmill workers) were the most common tasks being performed at the time of the fatal injury. Being struck by a falling tree, often one that had been disturbed by the tree being felled, was the most common mechanism of fatal injury. Isolation, poor work practice, errors of judgement, and equipment problems were the most common contributing factors to the fatal incidents. Emphasis on correct work practice and training (especially in felling), equipment maintenance, and availability of communication and medical retrieval in isolated areas are recommended.  相似文献   
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