Determination of heavy metals and metals levels in five fish species from I??kl? Dam Lake and Karaca?ren Dam Lake (Turkey)

The concentrations of heavy metals and metals (Zn, Pb, Bi, Cd, Ni, Co, Fe, Mn, Mg, Cu, Cr, Ca, Sr, Na, Li, K) were measured in muscle of five species Cyprinus carpio (from I??kl? dam), Scardinius erythrophthalmus (from I??kl? dam), Tinca tinca (from I??kl? dam), C. carpio (from Karaca?ren dam), Carassius carassius (from Karaca?ren dam) caught from I??kl? and Karaca?ren. The highest metal was Na (466.95 μg/g) in C. carassius, while the lowest levels were Fe (0.37 μg/g) and Cu (0.37 μg/g) in C. carpio from I??kl? dam. The mean concentrations μg/g d.w. some of heavy metals in muscle of C. carpio (from I??kl? dam), S. erythrophthalmus (from I??kl? dam), T. tinca (from I??kl? dam), C. carpio (from Karaca?ren dam), C. carassius (from Karaca?ren dam) were as follows: Cd 2.00-2.10-1.95-2.06-2.27, Zn 4.36-2.64-13.13-13.33-24.47, Pb 1.65-1.24-1.90-2.37-2.41, Co 2.46-2.37-2.48-2.58-2.80, Mg 138.51-154.35-218.59-204.58-202.16, Fe 0.37-2.62-3.17-3.01-12.33, respectively.     

Modeling of agricultural drought risk pattern using Markov chain and GIS in the western part of Bangladesh

The aim of the study is to assess the agricultural drought risk condition in the context of global climate change in the western part of Bangladesh that covers about 45% area of the country for the period of 1960–2011. Drought Index (DI) and Drought Hazard Index (DHI) have been calculated by Markov Chain analysis and that of Drought Vulnerability Index (DVI) from socioeconomic and physical indicators. The DI values show that the northern part in general is more drought-prone, having less crops prospect, whereas the southern part is less drought-prone with high crop potentiality. The probability of extreme drought occurrence increases in recent decades in some parts as a result the drought events become more frequent in the areas. The DHI ranges from 15 to 32, and northern part suffers from more extreme drought hazards than that of southern part. DVI also indicates that northern part is exposed to high to very high drought vulnerability as higher percentage of illiterate people are involved in agricultural practices and high percentage of irrigation to cultivable land, but southern part exposed to moderate to low vulnerability because of low values of vulnerability indicators. Finally, agricultural drought exists at high risk condition in northern part and low in southern parts and 21.63, 26.54 and 29.68% of the area poses very high, high and moderate risk, respectively. So, immediate adaptation measures are needed keeping in mind climate features like rainfall and temperature variability, drought risk and risk ranking to make viable adaptation measures.     

Assessment of imidacloprid toxicity on reproductive organ system of adult male rats

In the current study it was aimed to investigate the toxicity of low doses of imidacloprid (IMI) on the reproductive organ systems of adult male rats. The treatment groups received 0.5 (IMI-0.5), 2 (IMI-2) or 8 mg IMI/kg body weight by oral gavage (IMI-8) for three months. The deterioration in sperm motility in IMI-8 group and epidydimal sperm concentration in IMI-2 and IMI-8 groups and abnormality in sperm morphology in IMI-8 were significant. The levels of testosterone (T) and GSH decreased significantly in group IMI-8 compared to the control group. Upon treatment with IMI, apoptotic index increased significantly only in germ cells of the seminiferous tubules of IMI-8 group when compared to control. Fragmentation was striking in the seminal DNA from the IMI-8 group, but it was much less obvious in the IMI-2 one. IMI exposure resulted in elevation of all fatty acids analyzed, but the increases were significant only in stearic, oleic, linoleic and arachidonic acids. The ratios of 20:4/20:3 and 20:4/18:2 were decreased and 16:1n-9/16:0 ratio was increased. In conclusion, the present animal experiments revealed that the treatment with IMI at NOAEL dose-levels caused deterioration in sperm parameters, decreased T level, increased apoptosis of germ cells, seminal DNA fragmentation, the depletion of antioxidants and change in disturbance of fatty acid composition. All these changes indicate the suppression of testicular function.     

Online sorting of recovered wood waste by automated XRF-technology: part II. Sorting efficiencies

Sorting of waste wood is an important process practiced at recycling facilities in order to detect and divert contaminants from recycled wood products. Contaminants of concern include arsenic, chromium and copper found in chemically preserved wood. The objective of this research was to evaluate the sorting efficiencies of both treated and untreated parts of the wood waste stream, and metal (As, Cr and Cu) mass recoveries by the use of automated X-ray fluorescence (XRF) systems. A full-scale system was used for experimentation. This unit consisted of an XRF-detection chamber mounted on the top of a conveyor and a pneumatic slide-way diverter which sorted wood into presumed treated and presumed untreated piles. A randomized block design was used to evaluate the operational conveyance parameters of the system, including wood feed rate and conveyor belt speed. Results indicated that online sorting efficiencies of waste wood by XRF technology were high based on number and weight of pieces (70-87% and 75-92% for treated wood and 66-97% and 68-96% for untreated wood, respectively). These sorting efficiencies achieved mass recovery for metals of 81-99% for As, 75-95% for Cu and 82-99% of Cr. The incorrect sorting of wood was attributed almost equally to deficiencies in the detection and conveyance/diversion systems. Even with its deficiencies, the system was capable of producing a recyclable portion that met residential soil quality levels established for Florida, for an infeed that contained 5% of treated wood.     

Production and Purification of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Degrading Enzyme from Streptomyces sp. AF-111

A poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) degrading bacterial strain designated as AF-111 was isolated from sewage sludge sample. The bacterium was identified by 16S rRNA gene sequencing. The results revealed that strain AF-111 showed 99 % similarity with Streptomyces althioticus strain NRRL B-3981 and designated as Streptomyces sp. strain AF-111. An extracellular PHBV depolymerase enzyme was produced under optimized conditions and purified through ammonium sulphate fractionation and column chromatography. The enzyme was purified to homogeneity, indicated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and molecular weight was found to be approximately 51 kDa. Effect of temperature, pH, metal ions and inhibitors on the PHBV depolymerase activity was determined. The enzyme was stable at wide range of temperature (35–55 °C) and pH (6–8). PHBV depolymerase was stable in the presence of different metal ions except iron and zinc which had inhibitory effect on depolymerase activity. Both ethylenediamine teteracetic acid and phenylmethyl sulphonyl fluoride strongly inhibited enzyme activity which indicates that this enzyme belongs to the serine hydrolase family like other polyhydroxyalkanoate depolymerases. The results show that a depolymerase from strain AF-111 can effectively degrade PHBV, therefore, it can be applied in the process of biochemical monomer recycling.     

Non-target effect of organic insecticides: effect of two plant extracts on soil microbial biomass and enzymatic activities in soil

Efficacious botanical derivatives can provide an alternative to synthetic pesticides for organic farming systems. However, there is lack of information regarding the side effects of organic pesticides on key soil ecological processes. In this study, we investigated the effects of aqueous extracts from Urginea maritima and Euphorbia myrsinites exhibiting translaminar and systemic activity against pests on microbial biomass and enzymatic activities in soil. Two grams of plant material was extracted with 100 ml of water and then diluted 1:100, 2:100, and 4:100 with distilled water. Diluted plant extracts were applied around hypocotyl of tomato by soil drench. The effect of both plant extracts on microbial biomass C, amount of total N and organic C, and enzymatic activity in soil was significant. After the last application, the highest microbial biomass C was determined in the lowest U. maritima concentration (U 1:100). Soils treated with the highest concentration of U. maritima (U 4:100) had always lower SMBC content than control soil. All concentrations of E. myrsinites decreased microbial biomass C by 18% to 27% compared to the control. Total nitrogen and organic carbon decreased in soils without (control) and with treated U. maritima extract from first application to last application. Phosphatase, urease, and β-glucosidase activities were monitored in plant extract-treated soils. Except U. maritima 1:100 treatments of second and fourth applications, the other treatments of plant extracts negatively affected enzymatic activity in soil. U. maritima and E. myrsinites plant extracts exhibited different effects on soil microbial biomass and activity, probably because of their different chemical contents.     

Prediction of ambient PM10 and toxic metals using artificial neural networks

In this study, an artificial neural network is employed to predict the concentration of ambient respirable particulate matter (PM10) and toxic metals observed in the city of Jaipur, India. A feed-forward network with a back-propagation learning algorithm is used to train the neural network the behavior of the data patterns. The meteorological variables of wind speed, wind direction, relative humidity, temperature, and time are taken as input to the network. The results indicate that the network is able to predict concentrations of PM10 and toxic metals quite accurately.     

Use of rubber and bentonite added fly ash as a liner material

In many countries regulations require all hazardous waste disposal facilities to be lined with suitable impermeable barriers to protect against contamination. In this study, a series of laboratory tests on rubber and bentonite added fly ash were conducted. The aim of the tests was to evaluate the feasibility of utilizing fly ash, rubber and bentonite as a low hydraulic conductivity liner material. Type C fly ash was obtained from Soma thermal power plant in Turkey; rubber in pulverized form was waste from the retreading industry. To investigate the properties of rubber and bentonite added fly ash, hydraulic conductivity, leachate analysis, unconfined compression, split tensile strength, one-dimensional consolidation, swell and freeze/thaw cycle tests were performed. The overall evaluation of results have revealed that rubber and bentonite added fly ash showed good promise and a candidate for construction of a liner.     

Monitoring environmental pollution in Erzurum by chemical analysis of Scots pine (Pinus sylvestris L.) needles

Pollution distribution maps of copper (Cu), zinc (Zn) and lead (Pb) for Erzurum province were developed on the basis of chemical analysis of Scots pine (Pinus sylvestris L.) needles collected from randomly selected sampling points during 2 years. The maps show deposition zones for the studied elements and could help in identification of sources and directions of air pollution dispersion. This study indicated that vegetation in Erzurum was greatly endangered by sulphur dioxide (SO(2)), whereas Zn does not pose an immediate threat to vegetation in most of the country's territory. However, in the city center, main and secondary road agglomeration, very high pollution with Pb and Cu could limit growth. Higher levels of SO(2) measured in some areas of Erzurum might be harmful for human and animal health. Results of this study indicated that Scots pine needles were not contaminated by Zn.