Chemical composition and antimicrobial activities of the essential oil from the seeds of Enterolobium contortisiliquum (leguminosae)

Seeds of Enterolobium contortisiliquum were subjected to steam distillation to obtain a light yellow essential oil in a yield of 3 ml/kg of seeds. The major components of the oil were identified using gas chromatography/mass spectrometry (GC-MS) and were furfural, limonene, linalool, estragole, carvone, and apiole with carvone representing more than 50% of the total composition. Antimicrobial activities of the essential oil were determined against four species of gram positive bacteria (Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, Micrococcus luteus) and two gram negative bacteria (Klebsiella pneumoniae, Serratia Marcescencs). The essential oil inhibited the growth of all tested bacteria but was most effective against the gram positive bacteria. Chemicals that are responsible for the antibacterial effect of the essential oil were determined using the bio-autography thin layer chromatography (TLC) technique. The active compounds responsible for the activity were found to be carvone and estragole.     

Description of hydrogeologic heterogeneity and evaluation of radionuclide transport at an underground nuclear test

Realistic models of contaminant transport in groundwater demand detailed characterization of the spatial distribution of subsurface hydraulic properties, while at the same time programmatic constraints may limit collection of pertinent hydraulic data. Fortunately, alternate forms of data can be used to improve characterization of spatial variability. We utilize a methodology that augments sparse hydraulic information (hard data) with more widely available hydrogeologic information to generate equiprobable maps of hydrogeologic properties that incorporate patterns of connected permeable zones. Geophysical and lithologic logs are used to identify hydrogeologic categories and to condition stochastic simulations using Sequential Indicator Simulation (SIS). The resulting maps are populated with hydraulic conductivity values using field data and Sequential Gaussian Simulation (SGS). Maps of subsurface hydrogeologic heterogeneity are generated for the purpose of examining groundwater flow and transport processes at the Faultless underground nuclear test, Central Nevada Test Area (CNTA), through large-scale, three-dimensional numerical modeling. The maps provide the basis for simulation of groundwater flow, while transport of radionuclides from the nuclear cavity is modeled using particle tracking methods. Sensitivity analyses focus on model parameters that are most likely to reduce the long travel times observed in the base case. The methods employed in this study have improved our understanding of the spatial distribution of preferential flowpaths at this site and provided the critical foundation on which to build models of groundwater flow and transport. The results emphasize that the impacts of uncertainty in hydraulic and chemical parameters are dependent on the radioactive decay of specific species, with rapid decay magnifying the effects of parameters that change travel time.     

Competition between Native Populus deltoides and Invasive Tamarix ramosissima and the Implications for Reestablishing Flooding Disturbance

Abstract: Changes in historical disturbance regimes have been shown to facilitate non-native plant invasions, but reinstatement of disturbance can be successful only if native colonizers are able to outcompete colonizing invasives. Reintroduction of flooding in the southwestern United States is being promoted as a means of reestablishing Populus deltoides subsp. wislizenii , but flooding can also promote establishment of an introduced, invasive species, Tamarix ramosissima . We investigated competition between Populus and Tamarix at the seedling stage to aid in characterizing the process by which Tamarix may invade and to determine the potential ability of Populus to establish itself with competitive pressure from Tamarix . We planted seedlings of Tamarix and Populus in five ratios at three densities for a total of 15 treatments. The growth response of each species was measured in terms of height, above-ground biomass, and tissue concentrations of nitrogen and phosphorous. These measurements across treatments were modeled as three-dimensional response surfaces. For both species, Populus density was more important than Tamarix density for determining growth response. Both species were negatively affected by increasing numbers of Populus seedlings. Due to the larger size of the native Populus , we predict that its superior competitive ability can lead to its dominance when conditions allow native establishment. Our results suggest that even in the presence of an invader that positively responds to disturbance, reestablishment of historical flooding regimes and post-flood hydrology can restore this ecosystem by promoting its dominant plant species.     

Prevalent fecal contamination in drinking water resources and potential health risks in Swat, Pakistan

Fecal bacteria contaminate water resources and result in associated waterborne diseases.This study assessed drinking water quality and evaluated their potential health risks in Swat, Pakistan. Ground and surface drinking water were randomly collected from upstream to downstream in the River Swat watershed and analyzed for fecal contamination using fecal indicator bacteria(Escherichia coli) and physiochemical parameters(potential of hydrogen, turbidity, temperature, electrical conductivity, total dissolved solid, color, odor and taste). The physiochemical parameters were within their safe limits except in a few locations, whereas, the fecal contaminations in drinking water resources exceeded the drinking water quality standards of Pakistan Environmental Protection Agency(Pak-EPA),2008 and World Health Organization(WHO), 2011. Multivariate and univariate analyses revealed that downstream urbanization trend, minimum distance between water sources and pit latrines/sewerage systems, raw sewage deep well injection and amplified urban,pastures and agricultural runoffs having human and animal excreta were the possible sources of contamination. The questionnaire survey revealed that majority of the local people using 10–20 years old drinking water supply schemes at the rate of 73% well supply,13% hand pump supply, 11% spring supply and 3% river/streams supply, which spreads high prevalence of water borne diseases including hepatitis, intestinal infections and diarrhea, dysentery, cholera, typhoid fever, jaundice and skin diseases in children followed by older and younger adults.