PCB congener specific oxidative stress response by microarray analysis using human liver cell line

In this study we have examined the effect of exposure to different congeners of PCBs and their role in oxidative stress response. A metabolically competent human liver cell line (HepG2) was exposed with two prototype congeners of PCBs: coplanar PCB-77 and non-coplanar PCB-153. After the predetermined times of exposure (0–24 h) at 70 μM concentration, the HepG2 cells showed significant apoptotic changes by fluorescent microscopy after 12 h of exposure. Gene set enrichment analysis (GSEA) identified oxidative stress as the predominant enrichment. Further, paraquat assay showed that PCB congeners lead to oxidative stress to different extents, PCB-77 being more toxic. This study, with emphasis on all recommended microarray quality control steps, showed that apoptosis was one of the most significant cellular processes as a result of oxidative stress, but each of these congeners had a unique signature gene expression, which was further validated by Taqman real time PCR and immunoblotting. The pathways involved leading to the common apoptotic effect were completely different. Further in-silico analysis showed that PCB-153 most likely acted through the TNF receptor, leading to oxidative stress involving metallothionein gene families, and causing apoptosis mainly by the Fas receptor signaling pathway. In contrast, PCB-77 acted through the aryl hydrocarbon receptor. It induced oxidative stress through the involvement of cytochrome P450 (CYP1A1) leading to apoptosis through AHR/ARNT pathway.     

Potential of multivariate quantitative methods for delineation and visualization of ecoregions

Multivariate clustering based on fine spatial resolution maps of elevation, temperature, precipitation, soil characteristics, and solar inputs has been used at several specified levels of division to produce a spectrum of quantitative ecoregion maps for the conterminous United States. The coarse ecoregion divisions accurately capture intuitively-understood regional environmental differences, whereas the finer divisions highlight local condition gradients, ecotones, and clines. Such statistically generated ecoregions can be produced based on user-selected continuous variables, allowing customized regions to be delineated for any specific problem. By creating an objective ecoregion classification, the ecoregion concept is removed from the limitations of human subjectivity, making possible a new array of ecologically useful derivative products. A red-green-blue visualization based on principal components analysis of ecoregion centroids indicates with color the relative combination of environmental conditions found within each ecoregion. Multiple geographic areas can be classified into a single common set of quantitative ecoregions to provide a basis for comparison, or maps of a single area through time can be classified to portray climatic or environmental changes geographically in terms of current conditions. Quantified representativeness can characterize borders between ecoregions as gradual, sharp, or of changing character along their length. Similarity of any ecoregion to all other ecoregions can be quantified and displayed as a "representativeness" map. The representativeness of an existing spatial array of sample locations or study sites can be mapped relative to a set of quantitative ecoregions, suggesting locations for additional samples or sites. In addition, the shape of Hutchinsonian niches in environment space can be defined if a multivariate range map of species occurrence is available.     

Effects of lead-contaminated sediment and nutrition on mallard duckling brain growth and biochemistry

Day-old mallard (Anas platyryhnchos) ducklings received either a clean sediment (24%) supplemented control diet, Coeur d'Alene River Basin, Idaho (CDARB) sediment (3449 microg/g lead) supplemented diets at 12% or 24%, or a positive control diet (24% clean sediment with equivalent lead acetate to the 24% CDARB diet) for 6 weeks. The 12% CDARB diet resulted in a geometric mean concentration of 396 ppb (WW) brain lead with decreased brain protein and ATP concentrations but increased oxidized glutathione (GSSG) relative to the control diet. The 24% CDARB diet resulted in a concentration of 485 ppb brain lead with lower brain weight and ATP concentration than controls but higher concentrations of reduced glutathione (GSH) and calcium. Lead acetate accumulated twice as well as CDARB derived lead and resulted in histopathological lesions of the brain. With a combination of a suboptimal diet and 24% CDARB, brain lead concentration was higher (594 ppb) than with 24% CDARB in the standard diet, histopathological lesions became apparent and GSH was higher than suboptimal diet controls.     

Infiltration and adsorption of dissolved atrazine and atrazine metabolites in buffalograss filter strips

Vegetated filter strips (VFS) potentially reduce the off-site movement of herbicides from adjacent agricultural fields by increasing herbicide mass infiltrated (Minf) and mass adsorbed (Mas) compared with bare field soil. However, there are conflicting reports in the literature concerning the contribution of Mas to the VFS herbicide trapping efficiency (TE). Moreover, no study has evaluated TE among atrazine (6-chloro-N-ethyl-N'-isopropyl-[1,3,5]triazine-2,4-diamine) and atrazine metabolites. This study was conducted to compare TE, Minf, and Mas among atrazine, diaminoatrazine (DA, 6-chloro[1,3,5]triazine-2,4-diamine), deisopropylatrazine (DIA, 6-chloro-N-ethyl-[1,3,5]triazine-2,4-diamine), desethylatrazine (DEA, 6-chloro-N-isopropyl-[1,3,5]triazine-2,4-diamine), and hydroxyatrazine (HA, 6-hydroxy-N-ethyl-N'-isopropyl-[1,3,5]triazine-2,4-diamine) in a buffalograss VFS. Runoff was applied as a point source upslope of a 1- x 3-m microwatershed plot at a rate of 750 L h(-1). The point source was fortified at 0.1 microg mL(-1) atrazine, DA, DIA, DEA, and HA. After crossing the length of the plot, water samples were collected at 5-min intervals. Water samples were extracted by solid phase extraction and analyzed by high performance liquid chromatography (HPLC) photodiode array detection. During the 60-min simulation, TE was significantly greater for atrazine (22.2%) compared with atrazine metabolites (19.0%). Approximately 67 and 33% of the TE was attributed to Minf and Mas, respectively. These results demonstrate that herbicide adsorption to the VFS grass, grass thatch, and/or soil surface is an important retention mechanism, especially under saturated conditions. Values for Mas were significantly higher for atrazine compared with atrazine's metabolites. The Mas data indicate that atrazine was preferentially retained by the VFS grass, grass thatch, and/or soil surface compared with atrazine's metabolites.     

Desertification of the eastern Karoo,South Africa: Conflicting paleoecological,historical, and soil isotopic evidence

The desertification debate in South Africa has benefitted greatly in recent years from the contributions of a wide range of disciplines. In this paper we review the conflicting and supporting evidence for degradation in the eastern Karoo as reported in recent archaeological, historical, and stable carbon isotope studies as it relates to three key aspects of the debate: the precolonial environment, the rate and nature of change, and the relative contributions of humans and climate to the process. First, all studies suggest a greater grassiness at some time in the past, but researchers disagree on the timing of the switch to more shrubby conditions in the eastern Karoo. Second, regional rainfall records for the past 2 decades reveal an above-average rainfall period, and numerous long-term surveys show an increase in grass cover over the same period. These findings question the expanding Karoo hypothesis as well as the argument that the Karoo's carrying capacity has decreased in recent years. Finally, the relative responsibilities of humans and climate in the degradation process remain poorly understood and generally have not formed the focus of investigation.     

Lead in hawks,falcons and owls downstream from a mining site on the Coeur d'Alene River,Idaho

Mining and smelting at Kellogg-Smelterville, Idaho, resulted in high concentrations of lead in Coeur d'Alene (CDA) River sediments and the floodplain downstream, where American Kestrels (Falco sparverius), Northern Harriers (Circus cyaneus), Red-tailed Hawks (Buteo jamaicensis), Great Horned Owls (Bubo virginianus), and Western Screech-owls (Otus kennicotti) nested. Nestling American Kestrels contained significantly higher (P=0.0012) blood lead concentrations along the CDA River (0.24 µg/g, wet wt) than the nearby reference area (0.087 µg/g). A 35% inhibition of blood -aminolevulinic acid dehydratase (ALAD) in nestling Northern Harriers (P=0.0001), 55% in nestling American Kestrels (P=0.0001) and 81% in adult American Kestrels (P=0.0004) provided additional evidence of lead exposure in the CDA River population. In nestling American Kestrels and Northern Harriers, ALAD activity was negatively correlated with lead in blood. An earlier report on Ospreys (Pandion haliaetus) showed slightly less inhibition of ALAD than in American Kestrels, but no significant reduction in hemoglobin or hematocrit and no negative influence on production rates. The adult and nestling American Kestrels along the CDA River contained about twice as much blood lead as Ospreys during the same years (adult 0.46 vs. 0.20 µg/g, and nestling 0.24 vs. 0.09 µg/g), but adults showed a 7.5% reduction in hemoglobin (P=0.0356) and nestlings an 8.2% reduction in hemoglobin (P=0.0353) and a 5.8% reduction in hematocrit (P=0.0482). We did not observe raptor deaths related to lead, and although the production rate for American Kestrels was slightly lower along the CDA River, we found no significant negative relation between productivity and lead. Limited data on the other raptors provide evidence of exposure to lead along the CDA River. Several traits of raptors apparently reduce their potential for accumulating critical levels of lead which is primarily stored in bones of prey species.The U.S. Government right to retain a non-exclusive, royalty free licence in and to any copyright is acknowledged.     

Measurement and modelling of radioxenon plumes in the Ottawa Valley

Since 2001 a real-time radiation monitoring network of Canadian nuclear facilities and major population centres has been implemented for response to nuclear incidents including a possible terrorist attack. Unshielded NaI(Tl) spectroscopic detectors are employed to measure gamma radiation from airborne radioactivity and radioactivity deposited on the ground. These detectors are composed of a standard 3'x3' cylindrical NaI(Tl) spectrometers with data storage and integrated telemetry. Some of the detectors have been deployed in the Ottawa Valley near Chalk River Laboratories and Ottawa, which has a complex radioxenon environment due to the proximity of nuclear power reactors, and medical isotope facilities. Although not a health threat, these releases have provided an opportunity for the Canadian Meteorological Centre and the Commissariat à l'Energie Atomique to validate their meteorological models. The meteorological models of the two organizations are in good agreement on the origin and the source terms of these releases.     

Reconciling local control with appropriate scale in Norwegian moose management

In a landscape of fragmented private ownership, the need to coordinate game management across large areas presents challenges for landowners and public agencies alike. This paper describes how a recent reorganization of moose management in Norway achieves landscape-level planning while maintaining a tradition of local management by hunting teams. These two seemingly contradictory imperatives – coordinating wildlife management across large areas while keeping benefits and control in the hands of local resource users – are resolved through a nesting of management institutions, wherein the state serves a regulatory function and mid-level government (the county) serves to facilitate inter-local cooperation. This paper documents how the system is structured and describes the balance of incentives that enable the system to work. Information was gathered via interviews with staff at the Norwegian Directorate for Nature Management (now called the Norwegian Environment Agency), with wildlife management officials at the municipal level, with hunters, and from the most recent regulatory documents.     

Citizen-Science and Participatory Research as a Means to Improve Stakeholder Engagement in Resource Management: A Case Study of Vietnamese American Fishers on the US Gulf Coast

Environmental Management - This study examines the engagement of Vietnamese American commercial fisheries stakeholders in the US Gulf Coast with state and federal agencies and the role that citizen...