Potential dietary effects on the fatty acid composition of the common jellyfish Aurelia aurita

Fatty acid composition of the natural and aquarium-reared common jellyfish Aurelia aurita was investigated. Fatty acid composition of the aquarium-reared A. aurita clearly reflected that of the diet, brine shrimp (Artemia). In the same way, fatty acid composition of the natural A. aurita was assumed to reflect those of natural diets. Samples of natural A. aurita were collected from April 1995 to September 1995 in the Seto Inland Sea, Japan, and their fatty acids were analyzed by gas chromatography and mass spectrometry. Variation of fatty acid compositions was seasonal rather than dependent on body size. Two major seasonal groups were divided by the cluster analysis of the A. aurita fatty acid composition: the April–June and the August–September clusters. The April–June cluster was characterized by high contents of the (n − 3)-fatty acids of diatom origin, accumulated via the grazing food chain. By contrast, the August–September cluster was characterized by an increase in (n − 6)-fatty acids of macroalgal origin, probably transferred via the detritus food chain. These results suggest that the diet of natural A. aurita may shift between the diatom-based food chain and the detritus-based food chain. Received: 12 April 2000 / Accepted: 1 December 2000     

Possible involvement of the glycocalyx in the ichthyotoxicity of Chattonella marina (Raphidophyceae): immunological approach using antiserum against cell surface structures of the flagellate

Although the ichthyotoxic mechanism of Chattonella marina is still unknown, several lines of evidence suggest that the reactive oxygen species (ROS), such as superoxide anion (O₂^-), hydrogen peroxide (H₂O₂)_, and hydroxyl radical (·OH), produced by C. marina are involved in the mortality of fish exposed to this flagellate. Recently, we found that the cell-free supernatant prepared from C. marina, which is considered to contain the glycocalyx, showed NADPH-dependent O₂^- generation. In this study, we prepared antiserum against the crude glycocalyx of C. marina. Using indirect immunofluorescence, it was confirmed that the antiserum specifically reacted with C. marina cells. In addition to C. marina, the antiserum also reacted with other raphidophycean flagellates such as Heterosigma akashiwo, Olisthodiscus luteus, and Fibrocapsa japonica, whereas no reactivity was observed against six other flagellate species tested. These results suggest that raphidophycean flagellates have common epitopes recognized by the antiserum. Interestingly, immunohistochemical analysis of paraformaldehyde-fixed gill lamellae from yellowtail exposed to C. marina revealed that the antiserum stained the surface of gill lamellae, while no such staining pattern was observed in control gill lamellae. These results suggest that the glycocalyx may be discharged when C. marina cells are inhaled into the fishes' mouths and then come into contact with the gill surface. Based on the present results, together with our previous findings, we propose that continuous accumulation of the discharged glycocalyx on the gill surface occurs during C. marina exposure, which may be responsible for the ROS-mediated severe gill tissue damage leading to fish death.     

Quality assessment of groundwater from the south-eastern Arabian Peninsula

Assessment of groundwater quality plays a significant role in the utilization of the scarce water resources globally and especially in arid regions. The increasing abstraction together with man-made contamination and seawater intrusion have strongly affected groundwater quality in the Arabia Peninsula, exemplified by the investigation given here from the United Arab Emirates, where the groundwater is seldom reviewed and assessed. In the aim of assessing current groundwater quality, we here present a comparison of chemical data linked to aquifers types. The results reveal that most of the investigated groundwater is not suitable for drinking, household, and agricultural purposes following the WHO permissible limits. Aquifer composition and climate have vital control on the water quality, with the carbonate aquifers contain the least potable water compared to the ophiolites and Quaternary clastics. Seawater intrusion along coastal regions has deteriorated the water quality and the phenomenon may become more intensive with future warming climate and rising sea level.     

Effect of different soil layers on porewater to remediate acidic surface environment at a close mine site

This paper describes the chemistry of porewater when constructing different soil layers on acidic weathered rock of a closed mine to remediate the surface environment. Three cases were set on a flat surface of the site, all under different layer systems. Case 1 was only composed of weathered rocks. A top neutralization layer was constructed on the weathered rocks in case 2, whereas both an upper low-permeable and middle neutralization layers were constructed on the weathered rocks in case 3. The low-permeable layer of 30?cm thick consists of clay, and the neutralization layer of 30?cm thick consists of the mixture of the weathered rock and calcium carbonate as a neutralizer. Porewater sampling systems and soil sensors to measure temperature, water content, and electrical conductivity were set at different depths. In case 1, steadily high concentrations of heavy metals were observed regardless of the depth, and the pH ranged from 2 to 4. In cases 2 and 3, a dramatic decrease in concentrations of heavy metals was observed, even below the neutralization layer. For both cases, pH values were circumneutral. There were no significant seasonable changes in heavy metals concentrations and pH of porewater by considering the temperature and precipitation. In addition, the water content of the layers in case 3 fluctuated more mildly than that in cases 1 and 2, indicating that the low-permeable layer reduced the rate of infiltration. Therefore, a significant reduction in the load of heavy metals released from the site can be achieved by both implementing neutralization and low-permeable layers.     

Inexact Fuzzy-Stochastic Programming for Water Resources Management Under Multiple Uncertainties

In this study, an interval-parameter fuzzy-stochastic two-stage programming (IFSTP) approach is developed for irrigation planning within an agriculture system under multiple uncertainties. A concept of the distribution with fuzzy-interval probability (DFIP) is defined to address multiple uncertainties expressed as integration of intervals, fuzzy sets, and probability distributions. IFSTP integrates the interval programming, two-stage stochastic programming, and fuzzy-stochastic programming within a general optimization framework. IFSTP incorporates the pre-regulated water resources management policies directly into its optimization process to analyze various policy scenarios; each scenario has different economic penalty when the promised amounts are not delivered. IFSTP is applied to an irrigation planning in a water resources management system. Solutions from IFSTP provide desired water allocation patterns, which maximize both the system’s benefits and feasibility. The results indicate that reasonable solutions are generated for objective function values and decision variables; thus, a number of decision alternatives can be generated under different levels of stream flows.     

Assessment of metal pollution in urban road dusts from selected highways of the Greater Toronto Area in Canada

Over the last several decades, there has been increased attention on the heavy metal contamination associated with highways because of the associated health hazards and risks. Here, the results are reported of an analysis of the content of metals in roadside dust samples of selected major highways in the Greater Toronto Area of Ontario, Canada. The metals analysed are lead (Pb), zinc (Zn), cadmium (Cd), nickel (Ni), chromium (Cr), copper (Cu), manganese (Mn), calcium (Ca), potassium (K), magnesium (Mg) and iron (Fe). In the samples collected, the recorded mean concentrations (in parts per million) are as follows: Cd (0.51), Cu (162), Fe (40,052), Cr (197.9), K (9647.6), Mg (577.4), Ca (102,349), Zn (200.3), Mn (1202.2), Pb (182.8) and Ni (58.8). The mean concentrations for the analysed samples in the study area are almost all higher than the average natural background values for the corresponding metals. The geo-accumulation index of these metals in the roadside dust under study indicates that they are not contaminated with Cr, Mn and Ca; moderately contaminate with Cd and K; strongly contaminated with Fe and Mg; strongly to extremely contaminated with Ni and Pb; and extremely contaminated with Cu and Zn. The pollution load index (PLI) is used to relate pollution to highway conditions, and the results show that PLI values are slightly low at different samples collected from Highways 401 and 404 and high in many samples collected from Highway 400 and the Don Valley Parkway. Highway 400 exhibits the highest PLI values.     

Assessment using CFD of the wind direction on the air discharges caused by natural ventilation of a poultry house

Air inside poultry houses must be removed on a regular basis to prevent excess of heat, particles and noxious gases that can imperil animals. To cope with this issue, natural ventilation could be an effective method when assisted by accurate predictions. This study investigates air discharges caused by natural ventilation of a poultry house by means of a three-dimensional computational fluid dynamics (CFD) model. It solves the governing equations of momentum, heat and mass transport, radiative transfers and animal-generated heat. Wind directions of 0°, 36° and 56° (0° corresponds to a wind blowing perpendicular to the ridgeline) were investigated; the CFD model predictions achieved a RMSE of 1.2 °C and 0.6 g[H₂O] kg^?1 [dry air] for internal temperature and absolute humidity, respectively, when air blew with an angle of 36°. Air renewal rates (ARR) were 39.5 (±?1.9), 34.9 (±?2.2) and 33.6 (±?1.7) volumes of the building per hour, when air blew at 0°, 36° and 56°, respectively. Such ARR predictions served to know how the gases contained in air would likely spread downstream from the building in order to define regions of potentially high gas concentration that could endanger neighbouring habitable facilities.