In vivo detection of mutagenic effects of diesel exhaust by short-term mammalian bioassays

Male Chinese hamsters were exposed to diesel exhaust and clean air for six months at the Center Hill Facility of the U.S. Environmental Protection Agency in Cincinnati, Ohio. The animals were kept in specially constructed inhalation chambers and exposed to clean air or diesel exhaust for eight hours daily. The animals were sacrificed and slides prepared to study the mutagenic effects of diesel exhaust by four in vivo short term mammalian bioassays. Sperm morphology bioassay revealed a 2.67-fold increase in sperm abnormalities in the animals exposed to diesel exhaust as compared to those exposed to fresh air. Micronucleus bioassay revealed a 50% increase in the number of micronuclei in polychromatic erythrocytes obtained from animals exposed to diesel exhuast. However, no increase in sister chromatid exchange or chromosomal abnormalities was observed in bone marrow cells of animals treated with diesel exhaust. During these studies a decrease in mitotic index was observed in animals treated with diesel exhaust.     

Novel method for determining pyrene biodegradation using synchronous fluorimetry

To study the biodegradation rate of pyrene dissolved in liquid medium supplemented with mineral salts, a synchronous fluorimetry (SF) method was established. The limit of detection for pyrene dissolved in mineral salts medium (MSM) was determined as 0.19 ng/ml with a relative standard deviation of less than 1.3% (n = 9). The pyrene degrading rates of four bacterial strains were investigated using this method under the same experimental conditions. The degradation rates of the three active strains ranged from 76% to 87% after a 14-h incubation. The results were confirmed by the gas chromatography with a flame ionized detector (GC/FID) method. This implies that pyrene degradation can be directly monitored by the SF method without the solvent extraction of samples. The advantages of SF are that it is less laborious, faster, and less expensive than the GC/FID determination method with solvent extraction. The SF method provides a new tool for studying the degradation of polynuclear aromatic hydrocarbons (PAHs) in the natural environment and under experimental conditions.     

Atmospheric versus lithogenic contribution to the composition of first- and second-order stream waters in Seoul and its vicinity

The spatial variations in the chemistry of first- and second-order stream waters (N = 65) were investigated in the easterly bound of Seoul in order to assess the effects of urban air pollution on surface water chemistry. The sulfate (SO4(2-)) was high (range 3.9-17.8 mg l(-1), mean 11.8 mg l(-1)) within 30 km away from the center of Seoul, compared to the levels (range 1.1-7.7 mg l(-1), mean 4.3 mg l(-1)) observed in remote areas (30-70 km away). Both graphical examination and statistical evaluation (variogram) of sulfate concentration data consistently showed the decrease of sulfate concentration with increasing distance. The results of mass balance modeling also indicate that the concentrations of SO4(2-), Cl- and Na+ may be affected mainly by dry/wet deposition. However, the spatial variations of major cations such as Ca2+ and Na+ are well explained by the reaction of rainwater with diverse rocks in the watercourse. The water type was found to change from Ca(-Na)-SO4) type to Ca(-a)-HCO3 type with the increasing distance. It is thus inferred that the pollutants like SO2 emitted from strong man-made source areas of Seoul are transported to the considerable distance (at least 30 km away) by westerlies and that such mechanism may lead to the changes of the anion composition in surface water. In the remote area (> 30 km away from Seoul), the stream water chemistry appears to be influenced more effectively by the weathering of rock-forming minerals.     

Plant uptake and downward migration of 85Sr and 137Cs after their deposition on to flooded rice fields: lysimeter experiments with and without the addition of KCl and lime

In order to study the plant uptake and downward migration of radiostrontium and radiocesium deposited on to a flooded rice field, 85Sr and 137Cs were applied to the standing water over an acidic sandy soil in planted lysimeters. The plant uptake was quantified with the areal transfer factor (TFa, m2 kg(-1)-dry plant). Following the spiking 14 days after transplanting, the TFa values for the hulled seeds were 3.9 x 10(-4) for 85Sr and 1.4 x 10(-4) for 137Cs, whereas those for the straws were 1.3 x 10(-2) and 3.2 x 10(-4), respectively. The 137Cs TFa from the spiking at the anthesis/milky-ripe stage was several times higher than that from the earlier spiking, whereas the difference was much less in the 85Sr TFa. Such an increase in the 137Cs TFa was attributed mainly to an enhanced plant-base uptake. The addition of KCl and lime after the spiking significantly reduced the TFa values of both radionuclides. The reducing effect was greater for the later spiking. An appreciable fraction of the applied activity leached out of the lysimeter for 85Sr, whereas a negligible fraction leached for 137Cs. The leaching was remarkably increased by the KCl and lime addition for both. A conspicuous localization of 137Cs with respect to the soil surface was observed. In a batch experiment, the 137Cs concentration in the standing water decreased more rapidly than that of 85Sr, both of which were fitted to the power functions of the elapsed time. To add KCl and lime slowed such decreases to lessen the distribution coefficients (Kd) of both 85Sr and 137Cs.     

Vertical distribution and characteristics of soil humic substances affecting radionuclide distribution

The humic substances extracted from different soil depths are separated into humic (HA) and fulvic (FA) acids, and characterized for their chemical composition, proton exchange capacity, spectroscopic characteristics and binding properties to the europium ion. The chemical and spectroscopic results show that FA compared to HA has a relatively high O/C ratio, high acidic functional group contents and low aromatic contents. The synchronous fluorescence spectroscopic results show that the stability constant (K) of the soil humic substances with Eu(III) ion tends to increase as the soil depth becomes deeper, and HA has a slightly stronger binding ability than FA. The measured total site concentrations (C(L)) reveal that Eu(III) ion is loaded onto HA by 62-77% of the total acid sites, but FA is only approximately 50% covered by Eu(III) ion. Information could be useful in understanding the migration of radionuclides in soil layer.     

Riparian Ecosystem Management Model: Sensitivity to Soil,Vegetation, and Weather Input Parameters1

Abstract: The Riparian Ecosystem Management Model (REMM) was developed by the U.S. Department of Agriculture‐Agriculture Research Service (USDA‐ARS) and its cooperators to design and evaluate the efficiency of riparian buffer ecosystems for nonpoint source pollution reduction. REMM requires numerous inputs to simulate water movement, sediment transport, and nutrient cycling in the buffer system. In order to identify critical model inputs and their uncertainties, a univariate sensitivity analysis was conducted for nine REMM output variables. The magnitude of each input parameter was changed from ?50% to +50% from the baseline data in 12 intervals or, in some cases, the complete range of an input was tested. Baseline model inputs for the sensitivity analysis were taken from Gibbs Farm, Georgia, where REMM was tested using a 5‐year field dataset. Results of the sensitivity analysis indicate that REMM responses were most sensitive to weather inputs, with minimum daily temperature having the greatest impact on the nitrogen‐related outputs. For example, the 100% change (?50% to +50%) in minimum daily temperature input values yielded a 164.4% change in total nitrogen (N), a 109.3% change in total nitrate (NO₃), and a 127.1% change in denitrification. REMM was most sensitive to precipitation with regard to total flow leaving the riparian vegetative buffer zone (199.8%) and sediment yield (138.2%). Deep seepage (12.2%), volumetric water content (24.8%), and pore size index (6.5%) in the buffer soil profile were the most influential inputs for the output water movement. Sediment yield was most sensitive to Manning’s coefficient (46.6%), bare soil percent (40.7%), and soil permeability (6.1%). For vegetation, specific leaf area, growing degree day coefficients, and maximum root depth influenced the nitrogen related outputs. Overall results suggest that because of the high sensitivity to weather parameters, on‐site weather data is needed for model calibration and validation. The model’s relatively low sensitivity to vegetation parameters also appears to support the use of regional vegetation datasets that would simplify model implementation without compromising results.     

The relationships of distributive justice and compensation system fairness to employee attitudes in international joint ventures

Employee perceptions of the fairness of general corporate systems have thus far not received enough attention in the organizational justice literature. To fill this gap, we examined perceptions of the fairness of the compensation systems of international joint ventures in China. It is argued that perceptions of compensation system fairness are positively related to the three distributive justice dimensions, i.e., performance‐based distributive justice, comparative distributive justice relative to foreign expatriates, and comparative distributive justice relative to local colleagues. Perceptions of compensation system fairness are also hypothesized to mediate the relationships between the three distributive justice dimensions and organizational commitment and turnover intention. A survey of 161 Chinese employees in Sino‐foreign joint ventures found a positive relationship between perceptions of distributive justice and perceptions of compensation system fairness. In particular, compensation system fairness was more strongly associated with performance‐based distributive justice than with the other two distributive justice dimensions based on social comparison. The mediating role of compensation system fairness was strongly supported. Copyright © 2007 John Wiley & Sons, Ltd.     

Epidemiology of musculoskeletal symptoms among Korean hospital nurses.

We investigated the epidemiology of musculoskeletal symptoms (MSS) among a complete cross-section of 330 nurses from a large Korean hospital, by means of a questionnaire survey (response rate: 97.9%). The prevalence of MSS at any body site was 93.6%, with symptoms most commonly reported at the shoulder (74.5%), lower back (72.4%), neck (62.7%), lower legs (52.1%) and hand/wrist (46.7%). Logistic regression indicated that nurses who undertook manual handling of patients were 7.2 times as likely to report MSS (OR 7.2, 95%CI 1.2-42.3, P = .0275), while nurses suffering from periodic depression experienced a 3.3-fold MSS risk (OR 3.3, 95%CI 1.3-8.3, P = .0104). Overall, our study suggests that Korean nurses incur a very high MSS burden when compared internationally. A greater commitment is needed to improve physical conditions, occupational tasks and psychosocial work issues among nurses in this country.     

The effect of food availability on the semilunar courtship rhythm in the fiddler crab<Emphasis Type="Italic"> Uca lactea</Emphasis> (de Haan) (Brachyura: Ocypodidae)

Semilunar or lunar reproductive rhythms that follow tidal cycles are common in marine animals. For brachyuran crabs, an adaptive explanation for the synchronous release of larvae in phase with the tidal cycle is that females release larvae when their offspring are best able to escape predators. As a corollary to this hypothesis, the synchronous male reproductive cycle is selected to be in phase with female receptivity. As an alternative hypothesis, we propose that variation in food abundance influences the onset and intensity of the semilunar courtship cycle. We tested this hypothesis in male fiddler crabs ( Uca lactea) by experimentally manipulating food availability for 4 weeks. Food-supplemented males built more semidomes and waved for more days than did food-deprived males or untreated control males. Moreover, food-supplemented males began courtship earlier and the median courtship day advanced with an increase in food. Courtship intensity was not related to crab size. These results provide the first evidence in marine animals that changes in food abundance due to the tides, and hence in phase with the semilunar cycle, influence male reproductive rhythm.     

Preparation conditions and swelling equilibria of biodegradable hydrogels prepared from microbial poly(γ-glutamic acid) and poly(ε-lysine)

Biodegradable hydrogels prepared by -irradiation from microbial poly(amino acid)s are reviewed. pH-sensitive hydrogels were prepared by means of -irradiation of poly(-glutamic acid) (PGA) produced byBacillus subtilis IFO3335 and poly(-lysine) (PL) produced byStreptomyces albulus in aqueous solutions. The preparation conditions, swelling equilibria, hydrolytic degradation, and enzymatic degradation of these hydrogels were studied. A hydrogel with a wide variety of swelling behaviors has been produced by -irradiation from a mixture solution of PGA and PL.Paper presented at the 4th International Workshop on Biodegradable Plastics and Polymers, October 11–14, 1995, Durham, New Hampshire, USA.