821.
A signal-amplified mercury sensing biosensor with desired sensitivity was developed through firstly using the GFP mutant with fluorescence increasing response towards Hg
2+ as the reporter module. The developed biosensor showed response for Hg
2+ in a relatively wide range of 1–10,000 nmol/L, and the detection limit was improved one or two orders of magnitude in comparison with most metal-sensing biosensors in similar constructs. In addition, the biosensor could distinguish Hg
2+ easily from multiple metal ions and displayed strong adaptability to extensive pH conditions (pH 4.0–10.0). More importantly, the developed biosensor was able to provide an initial assessment of Hg
2+ spiked in the environmental water with the recoveries between 85.70% and 112.50%. The signal-amplified strategy performed by the modified reporter module will be widely applicable to many other whole-cell biosensors, meeting the practical requirements with sufficient sensing performance.
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