Can Volunteers Collect Data that are Comparable to Professional Scientists? A Study of Variables Used in Monitoring the Outcomes of Ecosystem Rehabilitation

Having volunteers collect data can be a cost-effective strategy to complement or replace those collected by scientists. The quality of these data is essential where field-collected data are used to monitor progress against predetermined standards because they provide decision makers with confidence that choices they make will not cause more harm than good. The integrity of volunteer-collected data is often doubted. In this study, we made estimates of seven vegetation attributes and a composite measure of six of those seven, to simulate benchmark values. These attributes are routinely recorded as part of rehabilitation projects in Australia and elsewhere in the world. The degree of agreement in data collected by volunteers was compared with those recorded by professional scientists. Combined results showed that scientists collected data that was in closer agreement with benchmarks than those of volunteers, but when data collected by individuals were analyzed, some volunteers collected data that were in similar or closer agreement, than scientists. Both groups’ estimates were in closer agreement for particular attributes than others, suggesting that some attributes are more difficult to estimate than others, or that some are more subjective than others. There are a number of ways in which higher degrees of agreement could be achieved and introducing these will no doubt result in better, more effective programs, to monitor rehabilitation activities. Alternatively, less subjective measures should be sought when developing monitoring protocols. Quality assurance should be part of developing monitoring methods and explicitly budgeted for in project planning to prevent misleading declarations of rehabilitation success.     

Simulating Land-Cover Change in Montane Mainland Southeast Asia

We used the conversion of land use and its effects (CLUE-s) model to simulate scenarios of land-cover change in Montane mainland southeast Asia (MMSEA), a region in the midst of transformation due to rapid intensification of agriculture and expansion of regional trade markets. Simulated changes affected approximately 10 % of the MMSEA landscape between 2001 and 2025 and 16 % between 2001 and 2050. Roughly 9 % of the current vegetation, which consists of native species of trees, shrubs, and grasses, is projected to be replaced by tree plantations, tea, and other evergreen shrubs during the 50 years period. Importantly, 4 % of this transition is expected to be due to the expansion of rubber (Hevea brasiliensis), a tree plantation crop that may have important implications for local-to-regional scale hydrology because of its potentially high water consumption in the dry season.     

Reforestation Strategies Amid Social Instability: Lessons from Afghanistan

Foreign and domestic government agencies and other international organizations pursue reforestation programs in rural upper watershed areas of Afghanistan over the past decade to alleviate poverty, combat the insurgency and rehabilitate a depleted forest resource base. Popular programs incorporate cash-for-work to conduct hillside terracing, check dam construction and tree-planting for nut production, fuel wood, timber, dune stabilization, and erosion abatement. Programmatic approaches have varied as a function of accessibility, security and local objectives. Uncertain land tenure and use rights, weak local environmental management capacity, and a focus on agricultural production to meet immediate needs limit interest, nationally and locally. Unreliable security, a lack of high quality tree planting stock, limited technical knowledge and coordination among government agencies, and poor security hamper program expansion. Reforestation success would be most likely where these issues are least acute. The Afghan government should focus on supporting community based natural resource management, developing and disseminating improved conservation tree nursery strategies, and promoting watershed management schemes that incorporate forestry, range management and agronomic production. Reforestation practitioners could benefit from the human and material resources now present as part of the international war effort. Successes and failures encountered in Afghanistan should be considered in order to address similar problems in insecure regions elsewhere when reforestation may help reverse environmental degradation and contribute to broader social stabilization efforts.     

Mixed-Grass Prairie Canopy Structure and Spectral Reflectance Vary with Topographic Position

Managers of the nearly 0.5 million ha of public lands in North and South Dakota, USA rely heavily on manual measurements of canopy height in autumn to ensure conservation of grassland structure for wildlife and forage for livestock. However, more comprehensive assessment of vegetation structure could be achieved for mixed-grass prairie by integrating field survey, topographic position (summit, mid and toeslope) and spectral reflectance data. Thus, we examined the variation of mixed-grass prairie structural attributes (canopy leaf area, standing crop mass, canopy height, nitrogen, and water content) and spectral vegetation indices (VIs) with variation in topographic position at the Grand River National Grassland (GRNG), South Dakota. We conducted the study on a 36,000-ha herbaceous area within the GRNG, where randomly selected plots (1?km² in size) were geolocated and included summit, mid and toeslope positions. We tested for effects of topographic position on measured vegetation attributes and VIs calculated from Landsat TM and Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) data collected in July 2010. Leaf area, standing crop mass, canopy height, nitrogen, and water content were lower at summits than at toeslopes. The simple ratio of Landsat Band 7/Band 1 (SR71) was the VI most highly correlated with canopy standing crop and height at plot and landscape scales. Results suggest field and remote sensing-based grassland assessment techniques could more comprehensively target low structure areas at minimal expense by layering modeled imagery over a landscape stratified into topographic position groups.     

The Control of an Invasive Bivalve, Corbicula fluminea, Using Gas Impermeable Benthic Barriers in a Large Natural Lake

Anoxia can restrict species establishment in aquatic systems and the artificial promotion of these conditions can provide an effective control strategy for invasive molluscs. Low abundances (2-20 m(-2)) of the nonnative bivalve, Asian clam (Corbicula fluminea), were first recorded in Lake Tahoe, CA-NV in 2002 and by 2010 nuisance-level population densities (>10,000 m(-2)) were observed. A non-chemical control method using gas impermeable benthic barriers to reduce dissolved oxygen (DO) concentrations available to C. fluminea was tested in this ultra-oligotrophic natural lake. In 2009, the impact of ethylene propylene diene monomer (EPDM) sheets (9 m(2), n = 6) on C. fluminea beds was tested on 1-7 day intervals over a 56 day period (August-September). At an average water temperature of 18 °C, DO concentrations under these small barriers were reduced to zero after 72 h resulting in 100 % C. fluminea mortality after 28 days. In 2010, a large EPDM barrier (1,950 m(2)) was applied to C. fluminea populations for 120 days (July-November). C. fluminea abundances were reduced over 98 % after barrier removal, and remained significantly reduced (>90 %) 1 year later. Non-target benthic macroinvertebrate abundances were also reduced, with variable taxon-specific recolonization rates. High C. fluminea abundance under anoxic conditions increased the release of ammonium and soluble reactive phosphorus from the sediment substrate; but levels of unionized ammonia were low at 0.004-0.005 mg L(-1). Prolonged exposure to anoxia using benthic barriers can provide an effective short term control strategy for C. fluminea.     

Enzymatic synthesis of bromo- and chlorocarbazoles and elucidation of their structures by molecular modeling

3-Chlorocarbazole, 3,6-dichlorocarbazole, dibromocarbazole, and 1,3,6,8-tetrabromocarbazole are emerging environmental contaminants which have been detected recently in water, sediment, and soil samples. However, their sources and occurrence have not been explained. Here, we report an enzymatic synthesis of bromo- and chlorocarbazoles by chloroperoxidase from Caldariomyces fumago in water. Density functional theory (DFT) method was used to predict the most stable products. Carbazole and chloroperoxidase were assayed in vitro in the presence of hydrogen peroxide, bromide, and chloride ions in different substrate ratio treatments against constant and varying enzyme concentrations. Halogenated carbazoles formed were identified by high-resolution gas chromatography coupled to mass spectrometry. In all treatments, bromination and chlorination took place, but the composition and concentration of compounds formed varied from one treatment to another. Mono-, di-, tri-, and tetra-substituted bromo- and chlorocarbazoles which include the reported environmental contaminants were synthesized. 3-Substituted and 3,6-substituted congeners were relatively higher in concentration. Enzyme concentration did not favor preferential formation of any of the compounds synthesized. However, their synthesis was influenced by halide concentration. Congeners with bromine and chlorine at position of C-3, C-3,6, C-1,3,6, and C-1,3,6,8 were calculated as the stable intermediate sigma complexes by DFT method. Regioselectivity in halogenation is discussed and hypothesis of the likely stable products in the environment explained. This study provides evidence that bromo- and chlorocarbazoles reported previously can be formed enzymatically in the environment, demonstrating the need to consider aromatic pollutants transformation and their potential toxicity enhancements in the management of water pollution and contaminated sites.     

Control of Asphaltic Concrete Plants in Los Angeles County

Characteristics of maximum short-term ground level concentrations from an elevated point source, namely, the effective plume height, the critical wind speed, the distance to the point of maximum concentration, and the maximum concentration, are derived from the gaussian plume model. Both phases of plume development—before and after it has reached its final height—are considered. The plume rise treatment includes both thermal buoyancy and momentum effects. Certain limitations on critical wind speed are discussed. The dispersion model whose basis is established in this paper should be especially useful in applications where on site meteorological data are unavailable.     

Problems in Judging Plume Opacity—A Simple Device for Measuring Opacity of Wet Plumes

The discoloration of exterior paint, has been shown to occur when H₂S in the air reacts with heavy metal salts used as pigments or fungicides in the paint. Simple field tests can give an indication whether H₂S was involved. An electron diffraction technique is described to identify the discolored compound in paint samples. Field experience in Jacksonville using this method is described.     

Effect of nitrogen fertilizer application on growing season soil carbon dioxide emission in a corn-soybean rotation

Nitrogen application can have a significant effect on soil carbon (C) pools, plant biomass production, and microbial biomass C processing. The focus of this study was to investigate the short-term effect of N fertilization on soil CO(2) emission and microbial biomass C. The study was conducted from 2001 to 2003 at four field sites in Iowa representing major soil associations and with a corn (Zea mays L.)-soybean (Glycine max L. Merr.) rotation. The experimental design was a randomized complete block with four replications of four N rates (0, 90, 180, and 225 kg ha(-1)). In the corn year, season-long cumulative soil CO(2) emission was greatest with the zero N application. There was no effect of N applied in the prior year on CO(2) emission in the soybean year, except at one of three sites, where greater applied N decreased CO(2) emission. Soil microbial biomass C (MBC) and net mineralization in soil collected during the corn year was not significantly increased with increase in N rate in two out of three sites. At all sites, soil CO(2) emission from aerobically incubated soil showed a more consistent declining trend with increase in N rate than found in the field. Nitrogen fertilization of corn reduced the soil CO(2) emission rate and seasonal cumulative loss in two out of three sites, and increased MBC at only one site with the highest N rate. Nitrogen application resulted in a reduction of both emission rate and season-long cumulative emission of CO(2)-C from soil.     

Estimated occupational risk from bioaerosols generated during land application of class B biosolids

Some speculate that bioaerosols from land application of biosolids pose occupational risks, but few studies have assessed aerosolization of microorganisms from biosolids or estimated occupational risks of infection. This study investigated levels of microorganisms in air immediately downwind of land application operations and estimated occupational risks from aerosolized microorganisms. In all, more than 300 air samples were collected downwind of biosolids application sites at various locations within the United States. Coliform bacteria, coliphages, and heterotrophic plate count (HPC) bacteria were enumerated from air and biosolids at each site. Concentrations of coliforms relative to Salmonella and concentrations of coliphage relative to enteroviruses in biosolids were used, in conjunction with levels of coliforms and coliphages measured in air during this study, to estimate exposure to Salmonella and enteroviruses in air. The HPC bacteria were ubiquitous in air near land application sites whether or not biosolids were being applied, and concentrations were positively correlated to windspeed. Coliform bacteria were detected only when biosolids were being applied to land or loaded into land applicators. Coliphages were detected in few air samples, and only when biosolids were being loaded into land applicators. In general, environmental parameters had little impact on concentrations of microorganisms in air immediately downwind of land application. The method of land application was most correlated to aerosolization. From this large body of data, the occupational risk of infection from bioaerosols was estimated to be 0.78 to 2.1%/yr. Extraordinary exposure scenarios carried an estimated annual risk of infection of up to 34%, with viruses posing the greatest threat. Risks from aerosolized microorganisms at biosolids land application sites appear to be lower than those at wastewater treatment plants, based on previously reported literature.