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141.
Vogel JR Stoeckel DM Lamendella R Zelt RB Santo Domingo JW Walker SR Oerther DB 《Journal of environmental quality》2007,36(3):718-729
Given known limitations of current microbial source-tracking (MST) tools, emphasis on small, simple study areas may enhance interpretations of fecal contamination sources in streams. In this study, three MST tools-Escherichia coli repetitive element polymerase chain reaction (rep-PCR), coliphage typing, and Bacteroidales 16S rDNA host-associated markers-were evaluated in a selected reach of Plum Creek in south-central Nebraska. Water-quality samples were collected from six sites. One reach was selected for MST evaluation based on observed patterns of E. coli contamination. Despite high E. coli concentrations, coliphages were detected only once among water samples, precluding their use as a MST tool in this setting. Rep-PCR classification of E. coli isolates from both water and sediment samples supported the hypothesis that cattle and wildlife were dominant sources of fecal contamination, with minor contributions by horses and humans. Conversely, neither ruminant nor human sources were detected by Bacteroidales markers in most water samples. In bed sediment, ruminant- and human-associated Bacteroidales markers were detected throughout the interval from 0 to 0.3 m, with detections independent of E. coli concentrations in the sediment. Although results by E. coli-based and Bacteroidales-based MST methods led to similar interpretations, detection of Bacteroidales markers in sediment more commonly than in water indicates that different tools to track fecal contamination (in this case, tools based on Bacteroidales DNA and E. coli isolates) may have varying relevance to the more specific goal of tracking the sources of E. coli in watersheds. This is the first report of simultaneous, toolbox approach application of a library-based and marker-based MST analyses to flowing surface water. 相似文献
142.
Microbial biomass governs enzyme activity decay during aging of worm-worked substrates through vermicomposting 总被引:2,自引:0,他引:2
Vermicomposting is the biooxidation and stabilization of organic matter involving the joint action of earthworms and microorganisms, thereby turning wastes into a valuable soil amendment called vermicompost. Studies have focused on the changes in the type of substrates available before and after vermicomposting, but little is known on how these changes take place, especially those changes related with maturation of vermicompost. This study investigated the effects of aging on the microbiological properties of fresh vermicompost produced from pig slurry by analyzing the substrate after the earthworms had left it. We incubated 16-wk-old vermicompost and sampled it after 15, 30, 45, and 60 d analyzing microbial biomass and activity (assessed as microbial biomass N and basal respiration respectively) and four enzymatic activities (beta-glucosidase, cellulase, protease, and alkaline phosphatase). Aging of vermicompost resulted in decreases of microbial biomass and activity. Three of the four enzymes analyzed also showed decrease. An initial increase followed by a rapid decrease in alkaline phosphatase was also recorded. High and significant correlations between microbial biomass and beta-glucosidase (r = 0.62, P < 0.001), cellulase (r = 0.56, P < 0.01), and protease (r = 0.82, P < 0.001) were found. Results suggest that there may be two steps involved in the aging dynamics of vermicompost with regards to extracellular enzyme activity; the first step was characterized by a decrease in microbial populations, which resulted in a reduction in the synthesis of new enzymes. The second step was the degradation of the pool of remaining enzymes. This dynamic does not seem to be affected by earthworms because similar decaying patterns of microbial biomass and activity were found in substrate where earthworms were present. 相似文献
143.
Relationship between Coefficient of Inbreeding and Parasite Burden in Endangered Gazelles 总被引:1,自引:0,他引:1
Jorge Cassinello Montserrat Gomendio Eduardo R. S. Roldan 《Conservation biology》2001,15(4):1171-1174
Abstract: We studied the effects of inbreeding depression on parasite infection in three species of endangered gazelles: Gazella cuvieri , G. dama, and G. dorcas . Coefficients of inbreeding were calculated for all individuals because complete genealogies were available. The levels of inbreeding differ both intra- and interspecifically. We collected samples of feces and determined nematode infection by counting nematode eggs in the samples. At the interspecific level, the species with the highest mean levels of inbreeding ( G. cuvieri ) had the highest levels of gastrointestinal parasites. Analyses done at the intraspecific level revealed a positive relationship between individual coefficient of inbreeding and parasite infection in G. cuvieri , but not in the species with the intermediate and lowest levels of inbreeding. Our findings suggest that high levels of inbreeding may make individuals more susceptible to parasitism, even under favorable environmental conditions, so this factor should be taken into account by those managing endangered species. 相似文献
144.
Edouard Lavergne Isabelle Calvès Anne Leila Meistertzheim Grégory Charrier Uwe Zajonz Jean Laroche 《Marine Biology》2014,161(5):1113-1126
Temporarily open/closed estuaries (TOCEs) are major ecosystems of the Indian Ocean coastal zones. Their functioning is tightly linked to climatic events such as monsoons and storms, and their mouth can close up for prolonged and variable periods of time, thus limiting their connectivity with the marine environment. Two types of genetic markers (i.e., mitochondrial cytochrome c oxidase I (COI) gene and microsatellites) were used to assess the genetic structure of 288 individuals of Terapon jarbua, a widely distributed fish species in the wider Gulf of Aden. Firstly, the hypothesis of panmixia was tested. Then, alternative hypotheses were investigated to explain the population genetic structure of T. jarbua: could it be shaped by (1) regional biogeographic barriers (i.e., Socotra Island vs. mainland Yemen) and/or (2) the particular functioning of TOCEs in relation to the species life cycle and particular physical ocean parameters? On one hand, the polymorphism of the COI inferred (1) a high haplotype diversity and a reduced nucleotide diversity over the whole data set and (2) a “star-like” shape of the haplotype network, thus suggesting a population expansion after local extinctions during the Pleistocene glaciations. On a second hand, the genotyping of eight microsatellites showed a significant genetic differentiation between T. jarbua populations in the wider Gulf of Aden (F ST = 0.035, p < 0.01), and thus, the panmixia hypothesis was rejected. Analyses of molecular variance results did not show any significant structure between Socotra Island and mainland Yemen and thus did not support the role of biogeographic barriers in structuring T. jarbua populations. Significant multi-locus deficits in heterozygotes at particular locations displaying high levels of F IS were recorded. It was suggested that a possible Wahlund effect took place in those TOCEs which could gather several cohorts of larvae stemming from different marine subpopulations over the sampled area. The present study emphasized the uniqueness of each TOCE as a potential reservoir of biodiversity and the urgent need for a better conservation program of those estuaries in the region, in order to avoid habitat fragmentation and permanent closure of those nursery areas by human activities. 相似文献
145.
146.
147.
Jorge Luis Frangi 《生态毒理学报》2000,22(2):75-78
为这片世界最南端的森林提出的一个主要森林管理和家具木材生产方案的可能影响,已经引起广泛关注.对这些原始森林的未来所给予的注意,突出说明了环境保护/生物多样性保护与经济效益之间的冲突.火地岛的公共当局和居民处在一个能指定他们合意的生活方式与环境质量的地位,而且能参与旨在以适合于其社会、经济和生态的条件和期望的方式确保可持续性的决策. 相似文献
148.
Marco Pandolfi Yolanda Gonzalez-Castanedo Andrés Alastuey Jesus D. de la Rosa Enrique Mantilla A. Sanchez de la Campa Xavier Querol Jorge Pey Fulvio Amato Teresa Moreno 《Environmental science and pollution research international》2011,18(2):260-269
Background
The impact of shipping emissions on urban agglomerations close to major ports and vessel routes is probably one of the lesser understood aspects of anthropogenic air pollution. Little research has been done providing a satisfactory comprehension of the relationship between primary pollutant emissions, secondary aerosols formation and resulting air quality. 相似文献149.
Echinoderms are major predators of anemones in temperate ecosystems. The fate of two algae, zooxanthellae and zoochlorellae, after their host anemone (Anthopleura elegantissima Brandt) was consumed by the leather star Dermasterias imbricata Grube was determined in experiments conducted in July and August 2004. Productivity, photosynthetic pigments, and mitotic index (percent of cells dividing) were used as indicators of algal health; algae released after leather stars consumed their host were compared with algae freshly isolated from anemones. Two types of waste products contained algae: pellets resulting from extraoral digestion, and feces. Zooxanthellae and zoochlorellae isolated from these waste products were photosynthetic, although to different extents. For algae from feces and pellets, light-saturated photosynthetic rates (P
max) were 85 and 13%, respectively, of P
max of freshly isolated zooxanthellae; and were 20 and 46%, respectively, for zoochlorellae. The photosynthetic pigments and mitotic index (percent of dividing cells) were not altered by the feeding activities of the leather star. These results show that algae released by seastar predation on their hosts remain viable, and are hence available for establishing symbioses in A. elegantissima and other potential hosts. 相似文献