Mutagenicity of particulate matter fractions in areas under the impact of urban and industrial activities

Organisms in the environment are exposed to a mixture of pollutants. Therefore the purpose of this study was to analyze the mutagenicity of organic and inorganic responses in two fractions of particulates (TSP and PM2.5) and extracts (organic and aqueous). The mutagenicity of organic and aqueous particulate matter extracts from urban-industrial and urban-residential areas was evaluated by Salmonella/microsome assay, through the microsuspension method, using strain TA98 with and without liver metabolization. Additionally, strains YG1021 and YG1024 (nitro-sensitive) were used for organic extracts. Aqueous extracts presented negative responses for mutagenesis and cytotoxicity was detected in 50% of the samples. In these extracts the presence of potential bioavailable metals was identified. All organic extracts presented mutagens with a higher potential associated with PM2.5. This study presents a first characterization of PM2.5 in Brazil, through the Salmonella/microsome assay. The evaluation strategy detected the anthropic influence of groups of compounds characteristically found in urban and industrial areas, even in samples with PM values in accordance with quality standards. Thus, the use of a genotoxic approach in areas under different anthropic influences will favor the adoption of preventive measures in the health/environment relation.     

Arsenic forensic modeling leads to decommission of downgradient/off-site monitoring wells

Arsenic was detected at concentrations exceeding the regulatory limit of 0.010 milligrams per liter (mg/L) in an off-site bedrock monitoring well downgradient of a former electroplating facility in Merrimack, New Hampshire. The bedrock underlying the site is associated with naturally occurring high concentrations of groundwater arsenic. Geochemical modeling was used to evaluate whether the arsenic in bedrock groundwater at the off-site monitoring location was site-related or naturally occurring. The hydrogeochemical signature of the off-site bedrock well did not resemble signatures of site-impacted bedrock wells. Multiple lines of evidence support that the arsenic observed in off-site bedrock groundwater was not a result of adverse impacts from site-related groundwater contamination.     

Pharmaceutical compound removal efficiency by a small constructed wetland located in south Brazil

Environmental Science and Pollution Research - The fate of pharmaceuticals during the treatment of effluents is of major concern since they are not completely degraded and because of their...     

Reflecting on the Anthropocene: The Call for Deeper Transformations: This article belongs to Ambio’s 50th Anniversary Collection. Theme: Anthropocene

Research on global environmental change has transformed the way that we think about human-environment relationships and Earth system processes. The four Ambio articles highlighted in this 50th Anniversary Issue have influenced the cultural narrative on environmental change, highlighting concepts such as “resilience,” “coupled human and natural systems”, and the “Anthropocene.” In this peer response, I argue that global change research is still paying insufficient attention to how to deliberately transform systems and cultures to avoid the risks that science itself has warned us about. In particular, global change research has failed to adequately integrate the subjective realm of meaning making into both understanding and action. Although this has been an implicit subtext in global change research, it is time to fully integrate research from the social sciences and environmental humanities.     

Effects of increased elevation and macro- and micronutrient additions onSpartina alterniflora transplant success in salt-marsh dieback areas in Louisiana

Spartina alterniflora was transplanted into dieback areas of a salt marsh in southeast Louisiana at two elevations (ambient and +30 cm) with and without macro- (N, P, and K) and micronutrient (Fe, Mn, Cu, and Zn) additions to determine if transplant success is dependent on increasing elevation or nutrients.Spartina alterniflora transplanted into elevated plots had more than twice the above- and belowground biomass as compared to nonelevated plots after three months of growth. Additionally, there was significantly more vegetative reproduction (greater culm density and number of newly produced culms) in elevated plots as compared to plots at ambient elevation. Macronutrient additions increased culm densities only in elevated plots.Spartina alterniflora transplanted into nonelevated plots had lower survival rates even when transplants received nutrient additions. These results suggest thatS. alterniflora may be transplanted successfully into degraded salt-marsh areas if elevation is increased. The addition of nutrients without a concomitant increase in elevation is not sufficient for transplant success.     

Protecting the piping plover under section 7 of the endangered species act

Section 7(a)(2) of the Endangered Species Act directs federal agencies to ensure that their actions do not jeopardize the continued existence of endangered and threatened species. The US Fish and Wildlife Service (USFWS) issues jeopardy or nonjeopardy biological opinions on proposed federal actions that affect endangered and threatened species. We summarize several biological opinions issued by the USFWS to protect the threatened piping plover (Charadrius melodus). These opinions address federal actions involving hundreds of piping plovers on the Missouri River system and a few piping plover pairs on short stretches of Atlantic coast beach. Some of these opinions are decisive, but most allow the proposed action to proceed conditional upon a lengthy set of reasonable and prudent alternatives to protect the piping plover. These conditions may prove difficult to track and will add to the workload of the USFWS.     

提供给热带森林管理的地方知识:植物次生化学节律和月亮周期的关系

本研究运用满月时收获非木材林产品的当地实践如识,来证明控制叶片分解速率的化学物质随月亮周期而波动,并且这种现象可能已发展为一种植物一食草动物之间的相互关系.当地知识认为,满月期收获的叶片更为耐用.满月期收获的棕榈叶片具有较高的总碳量、半纤维素和碳化合物、而钙的浓度则较低.这些化学上的变化使棕榈叶在满月收获时对食草动物不太敏感且更为耐用.本研究提出了热带植物减少食叶功物食用及影响衰老叶片的分解速度和耐用性(尤其在满月期)的作用机制.本研究认为森林经营中有必要利用天然的生命周期,并为当地人的收获实践提供了科学依据.     

Genetic amniocentesis in multiple gestations

Thirty-one genetic amniocenteses involving multiple gestations were performed in the genetics unit between 1976 and 1982. Three sets of triplets were included. Precise locations of the sacs were determined using real-time ultrasonography and successful sampling of all sacs was accomplished. Spontaneous abortions occurred in two normal twins and one normal triplet gestation. Two therapeutic abortions were performed for fetal abnormalities. Two cases of discordance for trisomy 21 (one twin and one triplet) were allowed to continue; the twin case terminated at 25 weeks' gestation with neonatal deaths and the triplets are alive and well.     

The Dynamics of Rural Vulnerability to Global Change: The Case of southern Africa

Research on the agricultural impacts of global change frequently emphasizesthe physical and socioeconomic impacts of climate change, yet globalchanges associated with the internationalization of economic activity mayalso have significant impacts on food systems. Together, climate change andglobalization are exposing farmers to new and unfamiliar conditions.Although some farmers may be in a position to take advantage of thesechanges, many more are facing increased vulnerability, particularly in thedeveloping world. This paper considers the dynamics of agriculturalvulnerability to global change through the example of southern Africa. Wedemonstrate that the combination of global and national economic changesis altering the context under which southern African farmers cope withclimate variability and adapt to long-term change. We find that farmers whoformerly had difficulty adapting to climatic variability may become lessvulnerable to drought-related food shortages as the result of tradeliberalization. At the same time, however, removal of national credit andsubsidies may constrain or limit adaptation strategies of other farmers,leaving them more vulnerable to climate variability and change.     

Proposed guidelines for diagnosis of chromosome mosaicism in amniocytes based on data derived from chromosome mosaicism and pseudomosaicism studies

Currently, accepted protocol which has been developed at the Prenatal Diagnosis Laboratory of New York City (PDL) requires that when a chromosome abnormality is found in one or more cells in one flask, another 20–40 cells must be examined from one or two additional flasks. Chromosome mosaicism is diagnosed only when an identical abnormality is detected in cells from two or more flasks. In a recent PDL series of 12 000 cases studied according to this protocol, we diagnosed 801 cases (6.68 per cent) of single-cell pseudomosaicism (SCPM), 126 cases (1.05 per cent) of multiple-cell pseudomosaicism (MCPM), and 24 cases (0.2 per cent) of true mosaicism. Pseudomosaicism (PM) involving a structural abnormality was a frequent finding (2/3 of SCPM and 3/5 of MCPM), with an unbalanced structural abnormality in 55 per cent of SCPM and 24 per cent of MCPM. We also reviewed all true mosaic cases (a total of 50) diagnosed in the first 22000 PDL cases. Of these 50 cases, 23 were sex chromosome mosaics and 27 had autosomal mosaicism; 48 cases had numerical abnormalities and two had structural abnormalities. Twenty-five cases of mosaicism were diagnosed in the first 20 cells from two flasks, i.e., without additional work-up, whereas the other 25 cases required extensive work-up to establish a diagnosis (12 needed additional cell counts from the initial two culture flasks; 13 required harvesting a third flask for cell analysis). Our data plus review of other available data led us to conclude that rigorous efforts to diagnose true mosaicism have little impact in many instances, and therefore are not cost-effective. On the basis of all available data, a work-up for potential mosaicism involving a sex chromosome aneuploidy or structural abnormality should have less priority than a work-up for a common viable autosomal trisomy. We recommend revised guidelines for dealing with (1) a numerical versus a structural abnormality and (2) an autosomal versus a sex chromosome numerical aneuploidy. Emphasis should be placed on autosomes known to be associated with phenotypic abnormalities. These new guidelines, which cover both flask and in situ methods, should result in more effective prenatal cytogenetic diagnosis and reduced patient anxiety.