Serotonin-induced mate rejection in the female cabbage butterfly, <Emphasis Type="Italic">Pieris rapae crucivora</Emphasis>

Virgin female cabbage butterflies, Pieris rapae crucivora, accept and mate with courting males, whereas mated females reject them and assume the “mate refusal posture”. This study tested whether the biogenic amines, serotonin (5HT), dopamine (DA), and octopamine (OA), were responsible for this change in behavior. The results showed that 2–3-day-old virgin females fed with 5HT rejected courting males significantly more frequently compared with controls fed on sucrose. In contrast, the proportions of courting males rejected by virgin females fed with either DA or OA did not differ from sucrose-fed controls. Oral application of each amine resulted in significantly increased levels of the amine applied (or its metabolite) in the brain. The results strongly suggest that 5HT or a 5HT metabolite may be responsible for the post-mating change in behavioral response of 2–3-day-old virgin females to courting males. Similar effects of 5HT treatment were observed in 6–8-day-old virgin females, but in this case the results were only marginally different from the controls, suggesting that the effect may decline with increasing female age.     

Characterization of compaction and CBR properties of recycled concrete aggregates for unbound road base and subbase materials in Vietnam

Journal of Material Cycles and Waste Management - Mechanical properties of recycled concrete aggregates (RCA) such as compaction and California bearing ratio (CBR) are important for application as...     

Featured Collection Introduction: Connectivity of Streams and Wetlands to Downstream Waters

Connectivity is a fundamental but highly dynamic property of watersheds. Variability in the types and degrees of aquatic ecosystem connectivity presents challenges for researchers and managers seeking to accurately quantify its effects on critical hydrologic, biogeochemical, and biological processes. However, protecting natural gradients of connectivity is key to protecting the range of ecosystem services that aquatic ecosystems provide. In this featured collection, we review the available evidence on connections and functions by which streams and wetlands affect the integrity of downstream waters such as large rivers, lakes, reservoirs, and estuaries. The reviews in this collection focus on the types of waters whose protections under the U.S. Clean Water Act have been called into question by U.S. Supreme Court cases. We synthesize 40+ years of research on longitudinal, lateral, and vertical fluxes of energy, material, and biota between aquatic ecosystems included within the Act's frame of reference. Many questions about the roles of streams and wetlands in sustaining downstream water integrity can be answered from currently available literature, and emerging research is rapidly closing data gaps with exciting new insights into aquatic connectivity and function at local, watershed, and regional scales. Synthesis of foundational and emerging research is needed to support science‐based efforts to provide safe, reliable sources of fresh water for present and future generations.     

Sustainable design of sanitation system based on material and value flow analysis for urban slum in Indonesia

Material flow analysis (MFA) and value flow analysis (VFA) were applied to the sanitation system in an urban slum in Indonesia. Based on the results of the MFA and VFA, garbage and excreta disposal costs were evaluated to be 0.7% and 1.1%, respectively, of per capita income. Such value flows seem reasonable in light of the recognized affordability to pay (ATP) standard. However, current excreta disposal methods create negative impacts on downstream populations. Because such disadvantages do not go back to disposers, but passed to downstream, the current value flow structure does not motivate individual toilet users to install treatment facility. Based on current material and value flow structures, a resource recycling sanitation system scenario was examined. Based on VFA, an affordable initial cost for such a system was calculated; this was found to be comparable in price to a cheaper composting toilet that is currently available in the market.     

Integrated biogeochemical modelling of nitrogen load from anthropogenic and natural sources in Japan

This study proposed an integrated biogeochemical modelling of nitrogen loads from anthropogenic and natural sources in Japan. Firstly, the nitrogen load (NL) from different sources such as crop, livestock, industrial plant, urban and rural resident was calculated by using datasets of fertilizer utilization, population distribution, land use map, and social census. Then, the nitrate leaching from soil layers in farmland, grassland and natural conditions was calculated by using a terrestrial nitrogen cycle model (TNCM). The Total Runoff Integrating Pathways (TRIP) was used to transport nitrogen from natural and anthropogenic sources through river channels, as well as collect and route nitrogen to the river mouths. The forcing meteorological and hydrological data is a 30-year (1976–2005) dataset for Japan which were obtained by the land surface model, Minimal Advanced Treatments of Surface Interaction and Runoff (MATSIRO). For the model validation, we collected total nitrogen (TN) concentration data from 59 rivers in Japan. As a comparison result, calculated TN concentration values were in good agreement with the observed ones, which shows the reliability of the proposed model. Finally, the TN loads from point and nonpoint sources were summarized and evaluated for 59 river basins in Japan. The proposed modelling framework can be used as a tool for quantitative evaluation of nitrogen load in terrestrial ecosystems at a national scale. The connection to land use and climate data provides a possibility to use this model for analysis of climate change and land use change impacts on hydrology and water quality.     

A new index for measuring perpendicularity of animal movements in relation to patch boundaries

We introduce a new index for measuring perpendicularity of animal movements with respect to a boundary (e.g., a habitat patch edge), and provide a computer algorithm for its calculation. Our index, η, improves on an approach that measures perpendicularity with respect to a fixed boundary direction. This is because η accounts for moment-to-moment trajectories relative to nearest-neighbor boundary attributes at the scale of an animal's movement. Our algorithm prp calculates η efficiently and accurately with both synthetic data and large telemetry datasets. In addition, we have included routines in prp which account for scenarios inherently problematic to perpendicularity estimators.     

AERCOARE: An overwater meteorological preprocessor for AERMOD

AERCOARE is a meteorological data preprocessor for the American Meteorological Society and U.S Environmental Protection Agency (EPA) Regulatory Model (AERMOD). AERCOARE includes algorithms developed during the Coupled-Ocean Atmosphere Response Experiment (COARE) to predict surface energy fluxes and stability from routine overwater measurements. The COARE algorithm is described and the implementation in AERCOARE is presented. Model performance for the combined AERCOARE-AERMOD modeling approach was evaluated against tracer measurements from four overwater field studies. Relatively better model performance was found when lateral turbulence measurements were available and when several key input variables to AERMOD were constrained. Namely, requiring the mixed layer height to be greater than 25 m and not allowing the Monin Obukhov length to be less than 5 m improved model performance in low wind speed stable conditions. Several options for low wind speed dispersion in AERMOD also affected the model performance results. Model performance for the combined AERCOARE-AERMOD modeling approach was found to be comparable to the current EPA regulatory Offshore Coastal Model (OCD) for the same tracer studies. AERCOARE-AERMOD predictions were also compared to simulations using the California Puff-Advection Model (CALPUFF) that also includes the COARE algorithm. Many model performance measures were found to be similar, but CALPUFF had significantly less scatter and better performance for one of the four field studies. For many offshore regulatory applications, the combined AERCOARE-AERMOD modeling approach was found to be a viable alternative to OCD the currently recommended model.

Implications: A new meteorological preprocessor called AERCOARE was developed for offshore source dispersion modeling using the U.S. Environmental Protection Agency (EPA) regulatory model AERMOD. The combined AERCOARE-AERMOD modeling approach allows stakeholders to use the same dispersion model for both offshore and onshore applications. This approach could replace current regulatory practices involving two completely different modeling systems. As improvements and features are added to the dispersion model component, AERMOD, such techniques can now also be applied to offshore air quality permitting.     

Evidence for bioamplification of nine polychlorinated biphenyl (PCB) congeners in yellow perch (Perca flavascens) eggs during incubation

This study investigated bioamplification of polychlorinated biphenyls (PCBs) in yellow perch (Perca flavescens) eggs resulting from nutrient utilization by developing embryos during incubation. Newly fertilized eggs containing trace levels of PCBs via maternal deposition were collected from an aquaculture pond in which adult broodstock had been reared over their natural lives. The eggs were incubated using a flow through system that received the same pond water at in-situ temperatures from which they were spawned. Replicate samples of eggs were collected at six time points throughout incubation, ranging from day 0 (newly fertilized eggs) to post-hatch larvae (2-d old). Congener specific PCB fugacities in pooled egg samples showed increases over the incubation period. Just prior to hatching, incubated eggs averaged 2.7-fold higher PCB fugacities compared to fresh eggs. The increase in PCB fugacity with egg incubation time was independent of chemical K_OW. After hatching, PCB residues were lost from the larvae, attenuating the maximum chemical fugacity achieved in late-incubated eggs. However, the rate of PCB elimination in the early larvae stages was K_OW dependent such that a significant larvae/egg fugacity ratio was still evident for intermediate and highly hydrophobic compounds 2 d post-hatching. This study provides the first evidence of in-ovo PCB bioamplification in eggs of an aquatic species and suggests that incubating fish embryos are exposed to higher chemical fugacities in-ovo than would be predicted by maternal deposition alone.