The application of bioassays as indicators of petroleum-contaminated soil remediation

Bioremediation has proven successful in numerous applications to petroleum contaminated soils. However, questions remain as to the efficiency of bioremediation in lowering long-term soil toxicity. In the present study, the bioassays Spirotox, Microtox, Ostracodtoxkit F, umu-test with S-9 activation, and plant assays were applied, and compared to evaluate bioremediation processes in heavily petroleum contaminated soils. Six higher plant species (Secale cereale L., Lactuca sativa L., Zea mays L., Lepidium sativum L., Triticum vulgare L., Brassica oleracea L.) were used for bioassay tests based on seed germination and root elongation. The ecotoxicological analyses were made in DMSO/H2O and DCM/DMSO soil extracts. Soils were tested from two biopiles at the Czechowice oil refinery, Poland, that have been subjected to different bioremediation applications. In biopile 1 the active or engineered bioremediation process lasted four years, while biopile 2 was treated passively or non-engineered for eight months. The test species demonstrated varying sensitivity to soils from both biopiles. The effects on test organisms exposed to biopile 2 soils were several times higher compared to those in biopile 1 soils, which correlated with the soil contaminants concentration. Soil hydrocarbon concentrations indeed decreased an average of 81% in biopile 1, whereas in biopile 2 TPH/TPOC concentrations only decreased by 30% after eight months of bioremediation. The bioassays were presented to be sensitive indicators of soil quality and can be used to evaluate the quality of bioremediated soil. The study encourages the need to combine the bioassays with chemical monitoring for evaluation of the bioremediation effectiveness and assessing of the contaminated/remediated soils.     

Bioaccumulation of elements in bryophytes from Serra da Estrela, Portugal, and Veluwezoom, the Netherlands

Background, Aims and Scope Pollution by heavy metals over large areas and long periods of time may cause chronic damage to living organisms and must be carefully controlled. One way to determine the extent of environmental contamination is by measuring the levels of contaminants in plants. The use of mosses as biomonitors is a convenient method to determine levels of (atmospheric) deposition, as terrestrial mosses obtain most of their supply of mineral elements from precipitation and dry deposition of airborne particles. Mosses have therefore received increasing attention as a suitable tool for monitoring regional patterns of elemental deposition from the atmosphere in large-scale studies in various countries, in areas close to industrial installations as well as in areas not expected to be contaminated. Although this technique is widely known, ecological studies of this type have rarely been done in Portugal. The aim of this paper is to evaluate and compare the spatial distribution of heavy metals in Hypnum cupressiforme, Pleurozium schreberi, Dicranum scoparium and Polytrichum piliferum collected from the Serra da Estrela natural park in Portugal and in the Veluwezoom natural park in the Netherlands. The selected species are the most widely used bryophytes for biomonitoring in the boreal region. The popularity of these species for this purpose is due to their wide ecological amplitude and distribution.Methods At 54 sampling sites in both nature parks, samples of Hypnum cupressiforme, Pleurozium schreberi, Dicranum scoparium and Polytrichum piliferum were collected. Plant digests were analysed for Al, Ba, Ca, Cr, Fe, K, Mg, Mn, Ni, Sr, V, Zn, Pb, Cu, Cd, N and P. Differentiations between sampling sites in terms of concentrations of elements in mosses were evaluated by ANOVA and the least significant difference was calculated. The normality of the analysed features was checked with the chi square test. After standardization, the matrix of 54 samples and 10 heavy metals was subjected to numerical classification to detect groups of samples with similar patterns of metal concentrations. The clustering algorithm was prepared with Ward’s method, and the City Block Manhattan method was used for the similarity measure. Metals and samples were also subjected to ordination to reveal possible gradients of heavy metal levels, using PCA. Correlations were calculated between concentrations of metals and factors 1 and 2, allowing the dependence between the concentration of metals and factors (factor loading) to be estimated.Results and Discussion All species examined in both areas contained elevated levels of Mn and Pb. For each particular species, concentrations of N, P and Pb were significantly higher at Serra da Estrela, while concentrations of Cu were significantly higher at the Veluwezoom. Mosses from Portugal and the Netherlands differed significantly mainly in the concentrations of Al, Ba, Cr, Fe, Mn, Ni, Pb and V. This differentiation did not exceed that within the mosses from Portugal.Conclusions Mosses from Portugal and the Netherlands differ significantly mainly in the concentrations of Al, Ba, Cr, Fe, Mn, Ni, Pb and V. This differentiation does not exceed the differentiation within the mosses from Portugal.Recommendation and Outlook Further research is required into the origin and deposition of the polluting elements in other environmental compartments.     

Estimation of energy demand of fermentation-based hydrogen production

The paper is concerned with estimation of heat and power consumption in a conceptual plant to produce hydrogen from sucrose-containing or starchy biomass by fermentation. A hydrogen plant connected with a sugar factory is regarded as the basic option; the sugar factory serves as a source of sucrose-containing thick juice for the hydrogen plant, where this feedstock is processed to hydrogen. As another option, a stand-alone hydrogen plant in which starch must initially be converted to fermentable glucose solution is considered. The values of key process parameters are assumed on the basis of preliminary experimental data. For both options of the hydrogen plant, heat consumption is estimated taking heat recovery in a heat exchanger network into account. Power consumption is estimated by calculating power needed for pumping of liquid and gaseous process media.     

Economic and environmental effects of unilateral climate actions

Unilateral climate policy can be detrimental to global climate protection. Our objective is to provide insight into such a policy, to quantify the risk of carbon leakage, and to investigate the effects related to potential anti-leakage measures. We analyze existing definitions of carbon leakage and propose an alternative, rigorous one, which is different in three respects. The definition is then tested using computable general equilibrium analysis of the global economy and decomposition analysis. We identify a list of parameters that affect not only the magnitude but also the sign of the carbon leakage rate. Manipulating elasticities of substitution suggests that carbon leakage can be either positive or negative. Computable general equilibrium models, which are widely applied, including by the European Commission in this area, should be transparent, and their assumptions call for careful validation. We find that emission limits are properly distributed between sectors covered by the European Union Emissions Trading System and other sectors for the first commitment period (ended in 2012) but not for the second one (ending in 2020), where the target for the non-trading sectors should be reduced relative to the target for the trading sectors in order to equlize marginal abatement costs.     

序半连续式反应器应用于生物废水处理中的氧传递模型及参数估值

根据ASM 1号模型中的原理和结论,推导出序半连续式反应器生物废水处理中的氧传递数学模型;在试验的基础上,对模型中的参数采用了合适的方法进行参数估值;在研究空气流量与氧的传递速率关系的基础上,得出了空气流量QA与氧传递系数KLa之间的线性关系式;研究了氧在反应器中的质量平衡.     

Age-related thermal strain in men while wearing radiation protective clothing during short-term exercise in the heat.

The aim of the study was to compare heat strain among different age groups of men in protective clothing during short-term physical work. Eight young (20-29 years), 6 middle-aged (41-55 years), and 6 older (58-65 years) men exercised for 30 min on a cycle ergometer (40% V(O2 max)) in 2 hot environments with a similar WBGT (ca. 26 degrees C): once with minimal clothing without infrared radiation (E1), and once with aluminized protective clothing under infrared radiation (E2). All subjects had sedentary jobs, but only the older subjects were physically active in their leisure-time. Body temperatures, heart rate, sweat rate, and subjective feelings were determined during the tests. Higher thermal strain was observed in E2 than in E1. No age-related differences in thermal strain were observed in either experiment indicating that active older men can tolerate short work periods with protective clothing in the heat as well as younger sedentary men.     

Accumulation of elements by edible mushroom species II. A comparison of aluminium,barium and nutritional element contents

The aim of the study was to compare accumulation efficiency of Al, Ba and nutritional elements (Ca, Fe, K, Mg, Mn, Na) exhibited by six edible mushrooms collected in particular regions of Poland during the last 20 years. The studied mushroom species were Boletus edulis, Cantharellus cibarius, Lactarius deliciosus, Leccinum aurantiacum, Suillus luteus and Xerocomus badius. The highest and the lowest concentrations of the elements in tested mushroom species were 11 – 410, 34 – 337, 16785 – 34600, 140 – 607, 12 – 75 and 16 – 143 mg kg^?1d.m., respectively. The highest average concentrations of Al, Mg and Mn were observed in Suillus luteus fruiting bodies, while for Ba, Ca, K and Na it was in Lactarius deliciosus. BCF >1 was found for K and Mg in all tested mushroom species and additionally for the highest Ca and Na concentrations of all tested mushroom species except for C. cibarius and S. luteus, respectively. For the other tested elements (Al, Ba, Fe and Mn) BCF values < 1 were recorded.     

MAGNETOHYDRODYNAMIC POWER GENERATION

The paper describes research and development in the field of magnetohydrodynamic power generation technology, based on discussions held in the Joint IAEA/UNESCO International Liaison Group on MHD electrical power generation. Research and development programmes on open cycle, closed cycle plasma and liquid-metal MHD are described. Open cycle MHD has now entered the engineering development stage. The paper reviews the results of cycle analyses and economic and environmental evaluations: substantial agreement has been reached on the expected overall performance and necessary component specifications. The achievement in the Soviet Union on the U-25 MHD pilot plant in obtaining full rated electrical power of 20.4 MW is described, as well as long duration testing of the integrated operation of MHD components. Work in the United States on coal-fired MHD generators has shown that, with slagging of the walls, a run time of about one hundred hours at the current density and electric field of a commercial MHD generator has been achieved. Progress obtained in closed cycle plasma and liquid metal MHD is reviewed. Electrical power densities of up to 140 MWe/m³ and an enthalpy extraction as high as 24 per cent have been achieved in noble gas MHD generator experiments.