Drinking water quality: An in vitro approach for the assessment of cytotoxic and genotoxic load in water sampled along distribution system

An in vitro approach was performed to assess the quality of drinking water collected at two treatment/distribution networks located near the source (Plant #1) and the mouth of River Po (Plant #2). The water was sampled at different points of each distribution network, before (raw water) and after the chlorine dioxide disinfection, and in two points of the pipeline system to evaluate the influence of the distribution system on the amount and quality of the disinfection by-product. Cytotoxicity and genotoxicity of water extracts were evaluated in human peripheral lymphocytes and Hep-G2 cells by the use of the micronucleus (MN) test and Comet assay. Raw water samples of both plants induced cytotoxic effects, but not the increases of MN frequency in Hep-G2 cells and in human lymphocytes. Increases of DNA damage in human leukocytes was detected by Comet assay for raw water of Plant #2 at concentration ≥ 0.25 Leq/mL. The disinfection process generally has reduced the toxicity of water samples, even if potential direct DNA-damaging compounds have been detectable in drinking water samples. The proposal approach, if currently used together with chemical analysis, can contribute to improve the monitoring drinking water.     

Real-life efficiency of urine source separation

Urine source separation (NoMix technology) is a promising innovation in wastewater management. To improve and further develop NoMix technology, it has been implemented in four Swiss households and at our research institute (Eawag). We conducted measurements during one year on frequency of toilet usage (in households 5.2/person/day for weekdays, and 6.3/person/day for weekends), flushing behavior (30–85% small flushes), and recovered urine. We calculate the amount of urine effectively recovered per voiding in NoMix toilets (138 ml/flush in households; 309 ml/flush in women's toilets at Eawag), and waterfree urinals (225 ml/usage). We estimate urine recovery in the households to be maximally 70–75% of the expected quantity, leaving room for technical and behavioral improvements. Based on sampling of N and P concentrations, we suspect nitrogen losses in the extended urine piping system. For households and workplaces, the daily and weekly flushing pattern is recorded. Our results are in accordance with literature data from a shorter period but with more people. These results represent a good dimensioning basis for future urine source separation applications. An example of extrapolation to an entire watershed is presented. The flushing pattern corresponds well with the typical nitrogen loading of a treatment plant.     

Local Irrigation Management Institutions Mediate Changes Driven by External Policy and Market Pressures in Nepal and Thailand

This article assesses the role of local institutions in managing irrigation water use. Fifty irrigation systems in each country were studied in Nepal and Thailand to compare the influence of local institutions on performance of irrigation systems amid changes in external policy and market pressures. Nepal’s new irrigation policy after the re-instatement of multiparty democracy in 1990 emphasized participatory irrigation management transferring the management responsibility from state authorities to water users. The water user associations of traditional farmer-managed irrigation systems were formally recognized by requiring registration with related state authorities. In Thailand also government policies encouraged people’s participation in irrigation management. Today water users are directly involved in management of even some large irrigation systems at the level of tertiary canals. Traditional communal irrigation systems in northern Thailand received support for system infrastructure improvement but have faced increased interference from government. In Thailand market development supported diversification in farming practices resulting in increased areas under high water-demanding commercial crops in the dry season. In contrast, the command areas of most irrigation systems in Nepal include cereal-based subsistence farming with only one-third having commercial farming. Cropping intensities are higher in Nepal than in Thailand reflecting, in part, differences in availability of land and management. In both countries local institutions play an important role in maintaining the performance of irrigation systems as external drivers and local contexts change. Local institutions have provided alternative options for irrigation water use by mediating external pressures.     

Heavy metals and couple fecundity, the LIFE Study

The effect of heavy metals at environmentally relevant concentrations on couple fecundity has received limited study despite ubiquitous exposure. In 2005-2009, couples (n = 501) desiring pregnancy and discontinuing contraception were recruited and asked to complete interviews and to provide blood specimens for the quantification of cadmium (μg L⁻¹), lead (μg dL⁻¹) and mercury (μg L⁻¹) using inductively coupled plasma-mass spectrometry. Couples completed daily journals on lifestyle and intercourse along with menstruation and pregnancy testing for women. Couples were followed for 12 months or until pregnant. Fecundability odds ratios (FORs) and 95% confidence intervals (CIs) were estimated adjusting for age, body mass index, cotinine, and serum lipids in relation to female then male exposures. FORs <1 denote a longer time to pregnancy. In adjusted models, reduced FORs were observed for both female cadmium (0.78; 95% CI 0.63-0.97) and male lead (0.85; 95% CI 0.73-0.98) concentrations. When jointly modeling couples’ exposures, only male lead concentration significantly reduced the FOR (0.82; 95% CI 0.68, 0.97), though the FOR remained <1 for female cadmium (0.80; 95% CI 0.64, 1.00). This prospective couple based cohort with longitudinal capture of time to pregnancy is suggestive of cadmium and lead’s reproductive toxicity at environmentally relevant concentrations.     

Assessment of compost quality for its environmentally safe use by means of an ecotoxicological test on a soil organism

A research project was carried out to evaluate toxicological effects of compost addition to agricultural soil using the earthworm Eisenia foetida (Annellida) as a representative organism of the soil fauna. Moreover, the correlation between compost biochemical stabilization and toxicity at different phases of the composting process was assessed. Samples were collected from three composting plants at three different maturation levels (beginning of the composting process, intermediate compost after bio-oxidation, and mature refined compost). Two tests were performed: a standard chronic solid-phase test and an acute solid-phase test (developed originally by the authors). In the first test, the measured end-points were mortality, growth and reproduction; while in the second test earthworms’ behavior was evaluated. The chosen compost concentrations in soil ranged from 2.5 to 100 %, with the aim of obtaining the toxicological parameters (LC50) and to mimic real agricultural dosages for the lower concentrations. Results indicated an increase in compost toxicity with greater compost concentrations; in particular, agricultural compost dosage below 10 % showed no toxicity. Moreover, toxicity did not decrease during composting; intermediate compost showed the highest LC50 values. As a consequence, no correlation was ascertained between the results of ecotoxicological analysis and waste biochemical stability parameters during the composting process.     

Mortality of the scleractinian coral <Emphasis Type="Italic">Cladocora caespitosa</Emphasis> during a warming event in the Levantine Sea (Cyprus)

A mortality event of Cladocora caespitosa corals and the extent of bleaching, necrosis and pigmented areas in the colonies were studied at the southeastern coast of Cyprus during a prolonged period of higher than average sea temperature anomalies (summer/autumn 2012). With the use of scuba diving and image analysis software, we monitored the extent of mortality of 29 colonies of C. caespitosa by measuring and comparing the area percentage of healthy tissue, affected tissue (bleached, necrotic) and older mortality events (encrusted skeleton). In September 2012, on average, 24 % of the colonies surface area was affected (bleaching and/or necrosis). In October 2012, C. caespitosa showed on average 26.3 % of the colony surface area affected, evidence of continuing deterioration. At the same time, 10 % (3 of 29) of the colonies showed an increase in the pigmentation of previously bleached polyps in small and marginal areas (6–8 %). Irrespective of the amount, the regaining of pigments recorded is considered an important find. Corals and marine organisms in general in the Levantine Sea are affected greatly by warming events, to the extent where a very small percentage of polyps/colonies show resilience under thermal stress. Natural bleaching of C. caespitosa, even though limited to a few colonies and very small portions of tissue/polyps, was documented for the first time in the Levantine Sea. We conclude that temperature anomalies are associated with the mortality event. Whether prolonged higher temperature is the direct cause, or whether it acts synergistically with other factors should be the subject of further investigations.     

Variation patterns in individual fish responses to chemical stress among estuaries, seasons and genders: the case of the European flounder (Platichthys flesus) in the Bay of Biscay

The objective was to describe and model variation patterns in individual fish responses to contaminants among estuaries, season and gender. Two hundred twenty-seven adult European flounders were collected in two seasons (winter and summer) in four estuaries along the Bay of Biscay (South West France), focusing on a pristine system (the Ster), vs. three estuaries displaying contrasted levels of contaminants (the Vilaine, Loire and Gironde). Twenty-three variables were measured by fish, considering the load of contaminants (liver metals, liver and muscle persistent organic pollutants, muscle polycyclic aromatic hydrocarbons); the gene expression (Cyt C oxydase, ATPase, BHMT, Cyt P450 1A1, ferritin); the blood genotoxicity (Comet test); and liver histology (foci of cellular alteration–tumour, steatosis, inflammation, abnormal glycogen storage). Canonical redundancy analysis (RDA) was used to model these variables using gender, season and estuary of origin as explanatory variables. The results underlined the homogeneity of fish responses within the pristine site (Ster) and more important seasonal variability within the three contaminated systems. The complete model RDA was significant and explained 35 % of total variance. Estuary and season respectively explained 30 and 5 % of the total independent variation components, whilst gender was not a significant factor. The first axis of the RDA explains nearly 27 % of the total variance and mostly represents a gradient of contamination. The links between the load of contaminants, the expression of several genes and the biomarkers were analysed considering different levels of chemical stress and a possible multi-stress, particularly in the Vilaine estuary.     

Institutional traps and vulnerability to changes in climate and flood regimes in Thailand

Vulnerabilities to floods in Thailand are changing as a result of many factors. Formal and informal institutions help shape exposure, sensitivity and capacities to respond of individuals, social groups and social-ecological systems. In this paper we draw on several case studies of flood events and flood-affected communities to first assess how current practices reflect various laws, procedures, programs and policies for managing floods and disasters and then explore the implications for dealing with additional challenges posed by climate change. Our analysis identifies several institutional traps which need to be overcome if vulnerability is to be reduced, namely capture of agendas by technical elites, single-level or centralized concentration of capacities, organizational fragmentation and overemphasis on reactive crisis management. Possible responses are to expand public participation in managing risks, build adaptive capacities at multiple levels and link them, integrate flood disaster management and climate change adaptation into development planning, prioritize risk reduction for socially vulnerable groups and strengthen links between knowledge and practice. Responses like these could help reduce vulnerabilities under current climate and flood regimes, while also improving capacities to handle the future which every way that unfolds.     

Biologic impact on the coastal belt of the province of Venice (Italy,Northern Adriatic Sea): preliminary analysis for the characterization of the bathing water profile

Background, aim and scope  

This paper presents a preliminary study of the water profile with reference to microbiological parameters, required by Directive 2006/07/EC (European Community 2006) concerning the management of bathing water quality, in the coastal belt of the Province of Venice (Italy, Northern Adriatic Sea). A historical database has been implemented with monitoring data for the period 2000–2006 (data on rivers, bathing and marine coastal waters and on the characterization of Wastewater Treatment Plant (WWTP) discharges) from the institutional activity of Veneto Regional Environmental Prevention and Protection Agency (ARPAV). An integrated areal analysis for the microbiological investigation of homogeneous stretches along the coast of the Province of Venice was performed for a preliminary characterization of the bathing water profile considering water quality status and existing pressure sources.