Rapid fetal karyotype from cystic hygroma and pleural effusions

Fluid from pleural effusion (n=2) and cystic hygroma (n=7) was obtained from eight fetuses, between 13 and 32 weeks of pregnancy at the time when a conventional prenatal diagnosis procedure was carried out. As these fluids contain lymphocytes, they were processed like peripheral blood. A karyotype was obtained in 4 days in both cases of pleural effusion and in four out of seven samples of cystic hygroma. An abnormal karyotype was detected in three of the four samples of cystic hygroma: two trisomies 21 and a monosomy X. Different parameters were evaluated in order to predict the feasibility of obtaining a cytogenetic diagnosis. Our data showed that if the amount of fluid obtained was ⩾4 ml and the initial lymphocyte count (ILC) was >0.2 × 10⁶ cells/ml, a cytogenetic diagnosis was possible from an initial concentration of cultured lymphocytes )ICCL) of >0.06 × 10⁶ cells/ml.     

灰色二阶预测模型GM（2，1）在排污收费中的应用

本文运用灰色理论建立了排污收费的灰色二阶预列模型GM(2，1)，给出了GM(2，1)模型精确求解方法，并以某地排污收费为例进行模型拟合。经检验该模型的精度比GM(1，1)模型精度高，最后指出了运用该模型的条件。该模型为环境管理宏观调控的有效实施提供了一种新型的方法和手段。     

Prenatal diagnosis of 21-hydroxylase deficiency by rflp analysis of the 21-hydroxylase,complement C4, and HLA class II genes

In 19 pregnancies at risk for 21-hydroxylase deficiency (21OHD) in 18 families with at lea one affected child, prenatal diagnosis was performed by RFLP analysis using the enzymi Taq I and EcoRI and the DNA probes specific for the 21 OH genes, the closely linke complement C4 genes and the highly polymorphic HLA class II genes DRB, DQB, and DPI For fetal DNA analysis either chorionic villi or cultivated amniotic cells were used. In all 1 cases, a clear prenatal diagnosis was possible either with the 21OH probe alone or in mo cases, by combining the results of the different closely linked loci.     

Dystrophin protein and rflp analyis for fetal diagnosis and carrier confirmation of duchenne muscular dystrophy

A pregnant woman with indeterminate Duchenne muscular dystrophy (DMD) carrier status, but with DMD diagnosed in her deceased brother (unavailable for study), presented for prenatal diagnosis, intending to continue the pregnancy only if proven unaffected with DMD with near absolute certainty. Creatine kinase (CK) assays to clarify carrier status were inconclusive. Male sex in the fetus was identified, but DNA restriction fragment length polymorphism (RFLP) analysis was not yet available to this centre to investigate the possible transmission of the DMD gene, and the pregnancy was terminated. Tissue histology and dystrophin protein analysis demonstrated the absence of DMD. In a situation with proven maternal carrier status, future fetal inheritance of the opposite maternal X chromosome would indicate the presence of DMD. However, maternal carrier status remained in doubt through a second pregnancy, even with RFLP studies, and was finally established when dystrophin analysis confirmed the presence of DMD in the second fetus. Histologic findings are presented, contrasting features in the two fetuses. The value of dystrophin analysis for establishing the diagnosis of fetal DMD, in this case proving maternal carrier status in a difficult situation, and for demonstrating DMD gene:RFLP haplotype relationships is illustrated.     

502清洁生产实践

分析台州市两家502企业清洁生产情况,总结经验和成效,提出进一步清洁生产潜力,促进行业节能降耗减污。     

紫木凼金矿开采过程中的环境效应:2.污染趋势分析

主要对紫木凼金矿区CN- 、As、Hg、Pb、Zn五个污染项目进行了趋势分析和污染预测。氰化物虽然是剧毒污染物 ,但它具有很强的自净降解作用 ,对矿区的污染影响不大 ,只是对于废液池的清洗外排必须先治理后排放。建立了As、Hg、Pb、Zn在各个污染途径中的定量分配模式。该模式表明 ,开采过程中As、Hg、Pb的大部分 ( 64 %以上 )进入废液池外排 ,其余的进入矿渣。在此基础上 ,对这些元素从 1 989年到 2 0 0 0年的开采总量、进入废液、土壤、积水坑的分量进行了计算 ,并给出了在堆场下伏粘土中各元素的饱和吸附量和开始外排的时间。     

麻石文丘里洗涤吸收器在APV尾气处理上的应用

介绍了ＡＰＶ喷雾干燥尾气的性质，原尾气系统存在的问题，改造中采取的措施、原理、流程和取得的效果。实践证明，用“麻石文丘里一次降温、洗涤—ＸＰ型塔板二次洗涤—旋流除雾器除雾”的改造方案，取代原“换热器降温—喷淋塔洗涤—折流脱水器脱水”的流程，取得了良好的降温、洗气和除雾效果，且工程费用低。     

Hepatic calcifications in a fetus with trisomy 9 that underwent cordocentesis

Foci of calcification were observed at autopsy in the liver of a fetus with complete trisomy 9 on which two cordocenteses had been performed. It is suggested that liver calcifications are a possible complication of the procedure. As several other cases of calcifications in the liver and other organs of fetuses with autosomal trisomies have been described without a history of cordocentesis, further studies should be carried out to determine whether fetuses with chromosomal anomalies are more prone to thrombus formation and embolization.     

重庆南山马尾松林衰亡与大气污染关系的讨论

重庆市南郊的南山是一个风景区,有林地约二万七千余亩,主要是马尾松林(Pinusmassoniana Lamb.),自八十年代起明显出现衰亡现象,目前约有85％马尾松植株受到不同程度的伤害,死亡率达35％左右.马尾松出现的症状是针尖坏死,针叶提早脱落,针长变短,树冠稀疏,枝条枯死,径向生长变弱等.