Variability in the spatial association patterns of sponge assemblages in response to environmental heterogeneity

Previous work on tropical sponge assemblages has provided strong evidence that sponges coexist on coral reefs through a diversity of positive and negative associations; however, the majority of this work has focused on Caribbean coral reefs. Here, we investigate the intra-phyletic spatial associations between the 20 most abundant sponge species at two sites experiencing different environmental regimes in the Wakatobi National Marine Park, Indonesia. We used a Monte Carlo simulation approach to compare the number of spatial associations between each species pair to that expected if species distribution patterns were non-associative (i.e. random). We found that sponges were predominately randomly distributed at the high coral cover site, whereas most sponges were negatively associated with other sponges at the sedimented, low coral cover site. We also found differences between distribution patterns for specific species at the two sites; a number of species that showed a random distribution pattern at the high coral cover site had negative association patterns at the low coral cover site. Our research supports recent ecological studies suggesting that interactions between species are unlikely to be homogenously distributed, as we found that some sponge species interactions differed depending on the environmental regimes in which they were found; this suggests that species interactions may be spatially variable. Finally, our results contrast with studies from elsewhere, as the sponge assemblages at these two sites in the Wakatobi appear to be dominated by negative associations and random distribution patterns rather than widespread competition.     

Diet modification to reduce phosphorus surpluses: a mass balance approach

Diet modification to reduce phosphorus (P) concentrations in manures has been developed in response to environmental concerns over P losses from animal agriculture to surface waters. We used USDA-NASS statistics on animal numbers and crop production to calculate county scale mass balances for manure P production, P removed in harvested portion of crops, and the potential effects of diet modification. Although spreading manure evenly over all crop acreage within a county is unlikely to occur, these calculations give a good indication as to the impact diet modification to reduce P can have at a regional or national scale. There was a high degree of regional variability in manure P surpluses (e.g., with the large crop acreages in the grain belt leading to large P offtake in crops preventing most P surpluses). In 89% of counties, there was a deficit of manure P relative to crop P removal; therefore there was a manure P surplus in 11% of counties. Diet modification decreased the percentage of states with a manure P surplus from 11 to 8%, a decrease of approximately 27%. Diet modification decreased the percentage of counties with the greatest surpluses of manure P (>30 kg ha(-1)) from 3% of all counties to 1%. Diet modification to decrease manure P is an important part of strategies to alleviate environmental concerns associated with surplus manure P in many areas, but additional strategies to deal with manure P surpluses are needed in some areas.     

Broiler diet modification and litter storage: impacts on phosphorus in litters, soils, and runoff

Modifying broiler diets to mitigate water quality concerns linked to excess phosphorus (P) in regions of intensive broiler production has recently increased. Our goals were to evaluate the effects of dietary modification, using phytase and reduced non-phytate phosphorus (NPP) supplementation, on P speciation in broiler litters, changes in litter P forms during long-term storage, and subsequent impacts of diets on P in runoff from litter-amended soils. Four diets containing two levels of NPP with and without phytase were fed to broilers in a three-flock floor pen study. After removal of the third flock, litters were stored for 440 d at their initial moisture content (MC; 24%) and at a MC of 40%. Litter P fractions and orthophosphate and phytate P concentrations were determined before and after storage. After storage, litters were incorporated with a sandy and silt loam and simulated rainfall was applied. Phytase and reduced dietary NPP significantly reduced litter total P. Reducing dietary NPP decreased water-extractable inorganic phosphorus (IP) and the addition of dietary phytase reduced NaOH- and HCl-extractable organic P in litter, which correlated well with orthophosphate and phytic acid measured by 31P nuclear magnetic resonance (NMR), respectively. Although dry storage caused little change in P speciation, wet storage increased concentrations of water-soluble IP, which increased reactive P in runoff from litter-amended soils. Therefore, diet modification with phytase and reduced NPP could be effective in reducing P additions on a watershed scale. Moreover, efforts to minimize litter MC during storage may reduce the potential for dissolved P losses in runoff.     

Dietary strategies for reduced phosphorus excretion and improved water quality

Cost effective feeding strategies are essential to deal with P surpluses associated with intensive animal agriculture and the consequent impact on water quality. Reduction of P overfeeding, use of feed additives to enhance dietary P utilization, and development of high available phosphorus (HAP) grains have all been shown to decrease fecal P excretion without impairing animal performance. Much progress has been made, but more research will be needed to refine these strategies to maximize reductions in P excretion while maintaining animal performance. Recent research has focused on the impact of modifying dietary P on the forms of P excreted and the mobility of P in soils amended with these manures, with strong treatment trends becoming evident in the literature. In general, dietary strategies have been developed that can effectively reduce the total P concentration in manures produced, and combining strategies usually leads to greater reductions than individual practices. However, the impact of different approaches on the solubility of P in manures and amended soils has been more variable. Soluble P remains of particular concern due to links between solubility of P in manure and P losses from manure-amended soils. In this paper, we outline the major strategies for reducing dietary P in different species, review the literature on the impact of these approaches on P forms in manures and amended soils, and discuss the potential beneficial effects on animal agriculture and the environment.     

Effect of mixing soil aggregates on the phosphorus concentration in surface waters

At any time, the phosphorus (P) concentration in surface waters is determined by a complex interaction of inputs of soluble P and sorption-desorption reactions of P with sediments. This study investigated what factors control P in solution when various soil aggregates were mixed, seen as being analogous to selective soil erosion events, transport, and mixing within river systems. Fifteen soils with widely differing properties were each separated into three aggregate size fractions (2-52 microm, 53-150 microm, and 151-2,000 microm). Resin P, water-soluble phosphorus (WSP), and the phosphorus buffer capacity (PBC = resin P/WSP) were measured for each aggregate size fraction and WSP was also measured for 11 mixes of the aggregate fractions. The smallest aggregates tended to be enriched with resin P relative to the larger aggregates and the whole soils, while the opposite was true for WSP. As the PBC was a function of resin P and WSP, the PBC was greatest in the 2- to 52-microm aggregate size fraction in most cases. When two aggregate size fractions were mixed, the measured WSP was always lower than the predicted WSP (i.e., the average of the WSP in the two individual aggregates), indicating that WSP released by one aggregate fraction could be resorbed by another aggregate fraction. This resorption of P may result in lower than expected solution P concentration in some surface waters. The strength with which an eroded aggregate can release or resorb P to or from solution is in part determined by that aggregate's PBC.     

Change in soluble phosphorus in soils following fertilization is dependent on initial Mehlich-3 phosphorus

There is a lack of information on how fertilization and initial Mehlich-3 phosphorus (M3P) interact to affect water soluble P (WSP) in soils. Our objectives were to (i) quantify the relationship between WSP and M3P for four textural diverse benchmark soils of North Carolina (NC) and (ii) quantify the change in WSP concentrations following P additions to soils over a wide range of initial M3P. Soils known to represent a wide range in M3P were collected from an Autryville loamy sand (loamy, siliceous, subactive, thermic Arenic Paleudults), Wasda muck (fine-loamy, mixed, semiactive, acid, thermic Histic Humaquepts), Georgeville silt loam (fine, kaolinitic, thermic Typic Kanhapludults), and Pacolet sandy clay loam (fine, kaolinitic, thermic Typic Kanhapludults) and analyzed for M3P, Fe, Al, and WSP. An incubation study was also conducted where four samples representing a range in M3P from each series were fertilized at rates of 150 and 300 kg P ha(-1), and WSP was measured at 1, 7, and 21 d after fertilization. The Wasda muck exhibited a change point at 115 mg P kg(-1) across a broad range of M3P concentrations (60-238 mg kg(-1)) while Autryville, Georgeville, and Pacolet series (with ranges in M3P of 32-328, 119-524, 0-1034 mg P kg(-1), respectively) maintained linear relationships between WSP and M3P. For the fertilized soils, significant increases in WSP occurred regardless of P rate. Yet, WSP concentrations were greater in soils with greater initial M3P. Thus, these data suggest that shifting animal waste applications to fields of relatively lower M3P concentrations would have an immediate impact on reducing risk for P losses, if all other factors are equal.     

Genotoxicity monitoring of freshwater environments using caged crayfish (Astacus leptodactylus)

Genotoxicity of freshwater pollution was assessed by measuring DNA damage in haemocytes of caged freshwater crayfish Astacus leptodactylus by the means of Comet assay and micronucleus test, integrated with the measurements of physiological (total protein concentration) and immunological (total haemocyte count) haemolymph parameters as biomarkers of undergone stress. Crayfish were collected at the reference site (River Mre?nica) and exposed in cages for 1 week at three polluted sites along the Sava River (Zagreb, Sisak, Krapje). The long term pollution status of these locations was confirmed by chemical analyses of sediments. Statistically significant increase in DNA damage measured by the Comet assay was observed at all three polluted sites comparing to the crayfish from reference site. In addition, native crayfish from the mildly polluted site (Krapje) cage-exposed on another polluted site (Zagreb) showed lower DNA damage than crayfish from the reference site exposed at the same location indicating adaptation and acclimatisation of crayfish to lower levels of pollution. Micronuclei induction showed similar gradient of DNA damage as Comet assay, but did not reach the statistical significance. Observed increase in total haemocyte count and total protein content in crayfish from polluted environments in the Sava River also confirmed stress caused by exposure to pollution. The results of this study have proved the applicability of caging exposure of freshwater crayfish A. leptodactylus in environmental genotoxicity monitoring using Comet assay and micronucleus test.     

Survey design for lakes and reservoirs in the United States to assess contaminants in fish tissue

The National Lake Fish Tissue Study (NLFTS) was the first survey of fish contamination in lakes and reservoirs in the 48 conterminous states based on a probability survey design. This study included the largest set (268) of persistent, bioaccumulative, and toxic (PBT) chemicals ever studied in predator and bottom-dwelling fish species. The U.S. Environmental Protection Agency (USEPA) implemented the study in cooperation with states, tribal nations, and other federal agencies, with field collection occurring at 500 lakes and reservoirs over a four-year period (2000–2003). The sampled lakes and reservoirs were selected using a spatially balanced unequal probability survey design from 270,761 lake objects in USEPA’s River Reach File Version 3 (RF3). The survey design selected 900 lake objects, with a reserve sample of 900, equally distributed across six lake area categories. A total of 1,001 lake objects were evaluated to identify 500 lake objects that met the study’s definition of a lake and could be accessed for sampling. Based on the 1,001 evaluated lakes, it was estimated that a target population of 147,343 (±7% with 95% confidence) lakes and reservoirs met the NLFTS definition of a lake. Of the estimated 147,343 target lakes, 47% were estimated not to be sampleable either due to landowner access denial (35%) or due to physical barriers (12%). It was estimated that a sampled population of 78,664 (±12% with 95% confidence) lakes met the NLFTS lake definition, had either predator or bottom-dwelling fish present, and could be sampled.     

The risk of harmful algal blooms (HABs) in the oyster-growing estuaries of New South Wales, Australia

The spatial and temporal variability of potentially harmful phytoplankton was examined in the oyster-growing estuaries of New South Wales. Forty-five taxa from 31 estuaries were identified from 2005 to 2009. Harmful species richness was latitudinally graded for rivers, with increasing number of taxa southward. There were significant differences (within an estuary) in harmful species abundance and richness for 11 of 21 estuaries tested. Where differences were observed, these were predominately due to species belonging to the Pseudo-nitzschia delicatissima group, Dinophysis acuminata, Dictyocha octonaria and Prorocentrum cordatum with a consistent upstream versus downstream pattern emerging. Temporal (seasonal or interannual) patterns in harmful phytoplankton within and among estuaries were highly variable. Examination of harmful phytoplankton in relation to recognised estuary disturbance measures revealed species abundance correlated to estuary modification levels and flushing time, with modified, slow flushing estuaries having higher abundance. Harmful species richness correlated with bioregion, estuary modification levels and estuary class, with southern, unmodified lakes demonstrating greater species density. Predicting how these risk taxa and risk zones may change with further estuary disturbance and projected climate warming will require more focused, smaller scale studies aimed at a deeper understanding of species-specific ecology and bloom mechanisms. Coupled with this consideration, there is an imperative for further taxonomic, ecological and toxicological investigations into poorly understood taxa (e.g. Pseudo-nitzschia).