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71.
Kaluzhnaya OV Belikov SI Schröder HC Wiens M Giovine M Krasko A Müller IM Müller WE 《Die Naturwissenschaften》2005,92(3):134-138
In a preceding study it has been reported that the freshwater sponge Lubomirskia baicalensis, living in Lake Baikal (East Siberia), is composed of spicules forming a characteristic pattern which follows radiate accretive growth. Here we report that the spicules are synthesized by the enzyme silicatein, a protein which is related to cathepsin L. The cDNAs for silicatein and the related cathepsin L were isolated and used as probes to show that the mRNA levels of silicatein in the bases of the spicule skeleton of the animals are low, while the mRNA level of cathepsin L in this region exceeds that of the growing zone. This is the first comprehensive study on the importance of the axial filament/silicatein as an essential structural and functional component determining the growth and stability of demosponge spicules.Note: Sequences from Lubomirskia baicalensis have been deposited in the EMBL/GenBank; cDNAs for the putative silicatein (LBSILICA) are deposited under the accession no. AJ786771 and for cathepsin L (LBCATL) under the accession no. AJ786770. 相似文献
72.
The analysis of genotypic shifts in field-exposed populations has been proposed as a tool for monitoring the environmental
impacts of contaminants. Previous laboratory experiments showed the occurrence of genotypic shifts induced by copper exposure
in samples of Hediste diversicolor from the Pialassa estuary (North Adriatic Sea, Italy). In order to test if genotype-tolerance responses observed were consistent
at larger spatial scales, populations of H. diversicolor were sampled in three haphazardly chosen estuaries along the Italian coast (Comacchio, Pialassa, Serchio) and exposed to
0.48 mg l−1 Cu2+ under laboratory conditions. Survival analysis procedures were used to test for possible relationships between time-to-death
and genotypes at three allozyme loci (ALD, FH, PGI). Genotype-tolerance responses observed at locus ALD were consistent among populations up to 2500 km distant, with genotype ALD
102/102 associated with the shortest survival times. Comparisons with previous laboratory experiments showed that responses to copper
stress at locus ALD were also consistent in time, with genotypes ALD
102/102 and ALD
100/102 associated with higher 96 h mortality ratios. Results suggested the occurrence of stable relationships between genotypes
at locus ALD and tolerance responses of H. diversicolor to copper stress. Conversely, genotype-tolerance responses at loci PGI and FH were not consistent either in space (among populations from the three estuaries) or in time (between laboratory experiments),
being possibly affected by site-specific features of populations or stochastic factors. Results show that genotype-tolerance
responses should be carefully verified at different spatial and temporal scales, before considering genotypic shifts among
the possible tools for monitoring the effects of environmental stressors. 相似文献
73.
Statistical power analysis has been used to determine the sensitivity of the mussel-watch programme in Hellenic seas, with particular focus placed on the data obtained for metal content in mussels from the Saronicos Gulf during the period 1985-1997. Sample collection was carried out quarterly. Soft tissues of 60-100 similarly sized mussels (Mytilus galloprovincialis) were pooled into six composite samples and analysed by Atomic Absorption Spectroscopy (AAS). The study concludes that the resolution of monitoring programmes employed thus far is sufficiently high to detect temporal trends related to changes in contaminant levels caused either by drastic environmental control (such as those imposed on point-source pollutants) or by sudden increases in contaminant load. However, the detection of more subtle trends, which may be expected in the near future as a result of global pollution-control policies, is likely to require the adoption of much more sensitive programmes. In addition, the review of up-to-date knowledge on metal biology in mussels suggests that factors related to growth and reproduction should be taken into account to remarkably increase the resolution of mussel-watch programmes. Indicative maximum values for components of error to be complied with during routine monitoring are suggested. 相似文献
74.
Marco Keiner 《Environment, Development and Sustainability》2005,6(4):379-392
The principle of sustainable development is now 15-year-old. There are a lot of definitions and models for its explanation — ranging from triangles and prisms to eggs — but still its sense is diffuse. Moreover, important aspects like equity are not sufficiently taken into account.The following article takes a critical look on sustainable development. It shows logical, systemic, philosophic and ethic reasons for the re-development of substantial parts of the principle of sustainability. Based on the proposed Principle of Good Heritage it provides a rough outline of a future Concept of Evolutionability, comprising a first tentative for a definition of evolutionable development, aiming at achieving a more appropriate and more workable mainstream view of sustainability.Readers should send their comments on this paper to: BhaskarNath@aol.com within 3 months of publication of this issue. 相似文献
75.
AbstractObjective: The number of e-bike users has increased significantly over the past few years and with it the associated safety concerns. Because e-bikes are faster than conventional bicycles and more prone to be in conflict with road users, e-bikers may need to perform avoidance maneuvers more frequently. Braking is the most common avoidance maneuver but is also a complex and critical task in emergency situations, because cyclists must reduce speed quickly without losing balance. The aim of this study is to understand the braking strategies of e-bikers in real-world traffic environments and to assess their road safety implications. This article investigates (1) how cyclists on e-bikes use front and rear brakes during routine cycling and (2) whether this behavior changes during unexpected conflicts with other road users.Methods: Naturalistic data were collected from 6 regular bicycle riders who each rode e-bikes during a period of 2 weeks, for a total of 32.5?h of data. Braking events were identified and characterized through a combined analysis of brake pressure at each wheel, velocity, and longitudinal acceleration. Furthermore, the braking patterns obtained during unexpected events were compared with braking patterns during routine cycling.Results: In the majority of braking events during routine cycling, cyclists used only one brake at a time, favoring one of the 2 brakes according to a personal pre-established pattern. However, the favored brake varied among cyclists: 66% favored the rear brake and 16% the front brake. Only 16% of the cyclists showed no clear preference, variously using rear brake, front brake, or combined braking (both brakes at the same time), suggesting that the selection of which brake to use depended on the characteristics of the specific scenario experienced by the cyclist rather than on a personal preference. In unexpected conflicts, generally requiring a larger deceleration, combined braking became more prevalent for most of the cyclists; still, when combined braking was not applied, cyclists continued to use the favored brake of routine cycling. Kinematic analysis revealed that, when larger decelerations were required, cyclists more frequently used combined braking instead of single braking.Conclusions: The results provide new insights into the behavior of cyclists on e-bikes and may provide support in the development of safety measures including guidelines and best practices for optimal brake use. The results may also inform the design of braking systems intended to reduce the complexity of the braking operation. 相似文献
76.
77.
A large body of research has developed on male ornaments and their role in sexual selection while female ornaments have been less investigated. This study focused on the presence, function, and possible role of a sexually dimorphic nuptial trait as an indicator of female quality in a fish species presenting conventional sex roles: the yellow patch on the belly of Knipowitschia panizzae females. Aquarium spawning experiments demonstrated that this ornament is due to dermal pigments, is displayed only when the female is ready to spawn, and is switched off within minutes from the end of egg deposition. This sexual trait is variable in size among females and indicates female fecundity relative to her own body size. As a consequence, female yellow belly appears to be a reliable indicator of female quality as measured by current fecundity. Field data on natural nests highlights that males usually perform parental care on only one egg batch at a time. The modality of egg deposition suggests that males may be limited in their potential reproductive rates by environmental factors. Male limitations in egg care could favour male choosiness and the evolution of female nuptial displays.Communicated by R. Cattaneo-Vietti, Genova 相似文献
78.
Marco Casu Daniela Casu Tiziana Lai Piero Cossu Marco Curini-Galletti 《Marine Biology》2006,149(5):1163-1174
The giant Mediterranean limpet Patella ferruginea Gmelin, 1791 is an endangered marine gastropod, whose range has progressively shrinked to few, restricted areas, due to intense human exploitation. We have studied the genetic structure of the species, in order to (1) gather information about the levels of genetic variability within and between natural populations of P. ferruginea collected in two Sardinian marine protected areas (MPAs) (Penisola del Sinis—Isola di Mal di Ventre and Isola dell’ Asinara), and (2) make an attempt to find relationships between ecological and biological attributes of the species and the genetic differentiation of the populations studied. The genetic study was carried out by means of the analysis of ten inter-simple sequence repeat (ISSR) primers on a total of 40 individuals collected at four localities. Genetic analysis evidenced (1) medium to high levels of within-population genetic variability, (2) a pattern of genetic structuring that varied with spatial scales, and (3) a strong genetic differentiation between the two MPAs. Although preliminary, these results suggest that gene flow may be present only at very small geographic scale, raising concerns on the future of the conservation of the species. 相似文献
79.
Mart-Jan Schelhaas Geerten Hengeveld Marco Moriondo Gert Jan Reinds Zbigniew W. Kundzewicz Herbert ter Maat Marco Bindi 《Mitigation and Adaptation Strategies for Global Change》2010,15(7):681-701
Risks can generally be described as the combination of hazard, exposure and vulnerability. Using this framework, we evaluated
the historical and future development of risk of fire and wind damage in European forestry at the national level. Fire risk
is expected to increase, mainly as a consequence of an increase in fire hazard, defined as the Fire Weather Index in summer.
Exposure, defined as forest area, is expected to increase slightly as a consequence of active afforestation and abandonment
of marginal agricultural areas. Adaptation options to fire risk should therefore aim to decrease the vulnerability, where
a change in tree species from conifers to broadleaves had most effect. Risk for wind damage in forests is expected to increase
mainly as a consequence of increase in exposure (total growing stock) and vulnerability (defined by age class and tree species
distribution). Projections of future wind climate indicate an increase in hazard (storminess) mainly over Western Europe.
Adaptation options should aim to limit the increase in exposure and vulnerability. Only an increase in harvest level can stop
the current build-up of growing stock, while at the same time it will lower vulnerability through the reduction of the share
of old and vulnerable stands. Changing species from conifers to broadleaves helps to reduce vulnerability as well. Lowering
vulnerability by decreasing the rotation length is only effective in combination with a high demand for wood. Due to data
limitations, no forecast of future fire area or damaged timber amount by storms was possible. 相似文献
80.
Arizzi Novelli Alessandra Picone Marco Losso Chiara Volpi Ghirardini Annamaria 《毒物与环境化学》2013,95(4-6):183-191
The aim of this study was to investigate the toxicity of total ammonia towards sea urchin bioassays, in order to elucidate the role of ammonia as confounding factor in sediment quality assessment studies. New toxicity data, expressed as EC50, NOEC and LOEC are reported for Paracentrotus lividus based on experiments at differing pH values. Results confirmed that sperm cell toxicity test is not very sensitive to ammonia, while total ammonia embryotoxicity is strictly pH dependent. Accurate observations of embryotoxic effects at increasing total ammonia concentrations evidenced progressive shifts from malformed plutei to gastrula and blastula blockages. Toxicity data obtained for P. lividus are comparable with previous results with other echinoid species. 相似文献