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961.
Previously published mtDNA cytochrome b sequences for Xyrichtys novacula (Linnaeus) and two morphologically distinct types of Xyrichtys larvae (Pisces: Labridae) included human cytochrome b sequence, presumably due to sample contamination and/or poor preservation of sample DNA. Those sequences had been used to
identify the two types of Xyrichtys larvae as X. novacula, but owing to the contamination, the identifications are invalid. Fresh specimens were collected: X.␣martinicensis (Cuvier and Valenciennes), X. novacula, X. splendens (Castelnau), and Xyrichtys sp. larvae of each of the two morphotypes (dorsal-forward eye and ventral-forward eye). The cytochrome b fragment was amplified from each specimen using the polymerase chain reaction. Comparison of the sequences with human cytochrome b sequence confirmed that the new sequences were not contaminated. The interspecific differences in Xyrichtys sequences were less than previously reported, but still greater than observed in many fish genera. Distance and parsimony
analyses indicated that X. novacula and X. martinicensis were more closely related to each other than to X. splendens. This conclusion differs from our previous conclusion that X. martinicensis and X. splendens were more closely related to each other than to X. novacula. Distance and parsimony analyses also demonstrated that both larval morphotypes were X. novacula and, thus, our previous conclusions regarding larval ecology and oceanographic transport remain unchanged.
Received: 12 March 1997 / Accepted: 24 October 1997 相似文献
962.
During early development in fish, phospholipase A2 (EC 3.1.1.4) regulates membrane lipid modifications, which relates to changes in environmental conditions and provision of
fatty acids required for metabolic energy substrates and prostaglandin biosynthesis. A method to analyze phospholipase A2 in rat tissues has been modified to measure its activity in embryonic Atlantic halibut (Hippoglossus hippoglossus L.). Egg and embryo samples were collected during the 1994 spawning season. Enzyme activity was undetectable at fertilization
but in 10-d embryos was 230 pmol mg−1 h−1 (at 20 °C) and increased by ∼120% at hatch (17-d). Significant alterations in the fatty acid composition of important phospholipids,
phosphatidylcholine (PC) and phosphatidylethanolamine (PE), were also observed. The content of some critical polyunsaturated
fatty acids, and the ratio of unsaturated/saturated fatty acids, declined significantly over development. Acyl-chain restructuring
mediated through the activity of phospholipase A2, coupled with other observed lipid changes (significant increases in the PC/PE ratio and cholesterol content), would produce
a decreased fluidity of membranes during embryonic development, coinciding with the predicted upward movement of larvae in
the water column. Arachidonic acid (20:4n-6) removed from PE could serve as a precursor for biosynthesis of 2-series prostaglandins, and eicosapentaenoic acid (20:5n-3) from PC is a likely source for other prostaglandin types. Despite removal of polyunsaturated fatty acids, there was an
overall increase in lipid and fatty acid concentration, which can be attributed to amino acid catabolism during early developmental
stages.
Received: 9 September 1996 / Accepted: 8 September 1997 相似文献
963.
Whole-body carbon (C), nitrogen (N) and phosphorus (P) content, and stable-isotope composition (13C:12C and 15N:14N), were followed during metamorphosis of bonefish (Albula sp.) larvae (leptocephali). Metamorphosing larvae depend entirely on endogenous carbon compounds (some of which contain N and P) as an energy source. Two fundamental questions are (1) Do the demands of extensive tissue remodeling during metamorphosis require the efficient retention of N and P during the catabolism of carbon compounds? (2) What effect does the lack of feeding have on stable-isotope composition? Our results showed that both C and N decreased by ~35 to 40%, reflecting the utilization of neutral lipid (triacylglycerols) and N-containing compounds (phosphatidylethanolamine and keratan sulfate glycosaminoglycan) as energy sources, and indicating that larvae have little or no capacity to retain N. Evidence suggested that collagen breakdown, measured as a loss of hydroxyproline content, also contributed to N loss. Stable-isotope ratios, expressed as 13C and 15N, showed no statistically significant differences in early and advanced metamorphosing larvae. In contrast to C and N, phosphorus was conserved during metamorphosis and most probably is utilized in the increased bone mineralization occurring in advanced larvae. We show, however, that advanced larvae are P-limited and that normal ossification is dependent upon a supply of exogenous P obtained after the resumption of feeding. The N:P ratio of 12.3 in early larvae decreased to 8.1 in advanced larvae owing to the conservation of P as N was lost. The mean 15N value in early metamorphic larvae (11.6‰) is consistent with results from other studies, and provides further support for the view that premetamorphic leptocephali feed at a very low trophic level. 相似文献
964.
The pattern of settlement over time of three broadcast spawning coral species (Cyphastrea serailia, Acanthastrea lordhowensis, and Goniastrea australensis) from the Solitary Islands (30°00′S; 153°20′E) was studied in 1995 and 1996 in order to determine the maximum length of time
these larvae could remain in the water column and still retain the ability to settle and metamorphose. Larvae were maintained
in aquaria and the number which had settled on biologically-conditioned tile pairs was monitored every 5 to 10 d. While the
majority of larvae settled quickly after becoming competent, some larvae survived and settled for extended periods after spawning.
Competency periods ranged from 26 d for C. serailia to 56 d for G. australensis and 78 d for A. lordhowensis. These data greatly extend the known competency periods for larvae of broadcast-spawning corals and indicate the potential
for transport of broadcast-spawned coral larvae over large distances. Medium to long-distance larval dispersal of the species
studied provides a mechanism for their widespread distribution in subtropical regions, on reefs which are often widely spaced
and relatively isolated.
Received: 27 May 1997 / Accepted: 27 November 1997 相似文献
965.
Karyotypic characterization of the great sturgeon, Huso huso, by multiple staining techniques and fluorescent in situ hybridization 总被引:1,自引:0,他引:1
F. Fontana J. Tagliavini L. Congiu M. Lanfredi M. Chicca C. Laurente R. Rossi 《Marine Biology》1998,132(3):495-501
A karyotype analysis by several staining techniques was carried out on the great sturgeon, Huso huso (Linnaeus, 1758). The karyotype (2n = 118 ± 2) was composed of 42 pairs of meta-/submetacentric chromosomes and 17 pairs of acrocentrics/microchromosomes. Constitutive
heterochromatin was mainly located at the centromeric regions of the acrocentric chromosomes. The biarmed chromosomes showed
weak C-bands. Fluorescent staining with GC-specific chromomycin A3 showed clearly recognizable fluorescent regions, whereas a more uniform base composition was revealed by the AT-specific
4,6-diamidino-2 phenylindole. After Ag-staining, nucleolar organizer regions could be observed on the short arms of two medium-sized
submetacentrics and on two acrocentrics. Digoxigenated 28S and 5S rDNA probes, prepared from Acipenser naccarii DNA and hybridized to metaphase chromosomes, showed signals on six and two chromosomes, respectively. The telomeric sequence
(TTAGGG)
n
detected by FISH was located at both ends of each chromosome. Results are discussed in relation to karyotype organization
and evolution in sturgeons.
Received: 1 April 1998 / Accepted: 21 July 1998 相似文献
966.
Jürgen Gadau Pia J. Gertsch Jürgen Heinze Pekka Pamilo Bert Hölldobler 《Behavioral ecology and sociobiology》1998,44(1):23-33
Multilocus DNA fingerprinting and microsatellite analysis were used to determine the number of queens and their mating frequencies
in colonies of the carpenter ant, Camponotus ligniperdus (Hymenoptera: Formicidae). Only 1 of 61 analyzed queens was found to be double-mated and the population-wide effective mating
frequency was therefore 1.02. In the studied population, 8 of 21 mature field colonies (38%) contained worker, male, or virgin
queen genotypes which were not compatible with presumed monogyny and therefore suggested oligogyny, i.e., the cooccurrence
of several mutually intolerant queens within one colony. Estimated queen numbers in oligogynous colonies ranged between two
and five. According to the results of the genetic analysis, most of the queens coexisting in oligogynous colonies were not
closely related. Pleometrosis is very rare and queenless colonies adopt mated queens both in the laboratory and field. Therefore,
the most plausible explanation for the origin of oligogynous colonies in C. ligniperdus is the adoption of unrelated queens by orphaned mature colonies. The coexistence of unrelated, but mutually intolerant queens
in C. ligniperdus colonies demonstrates that oligogyny should be considered as a phenomenon distinct from polygyny.
Received: 18 December 1997 / Accepted after revision: 20 June 1998 相似文献
967.
Jacobus C. Biesmeijer Mark G. L. van Nieuwstadt Saskia Lukács Marinus J. Sommeijer 《Behavioral ecology and sociobiology》1998,42(2):107-116
Social insect foragers have to make foraging decisions based on information that may come from two different sources: information
learned and memorised through their own experience (“internal” information) and information communicated by nest mates or
directly obtained from their environment (“external” information). The role of these sources of information in decision-making
by foragers was studied observationally and experimentally in stingless bees of the genus Melipona. Once a Melipona forager had started its food-collecting career, its decisions to initiate, continue or stop its daily collecting activity
were mainly based upon previous experience (activity on previous days, the time at which foraging was initiated the day(s)
before, and, during the day, the success of the last foraging flights) and mediated through direct interaction with the food
source (load size harvested and time to collect a load). External information provided by returning foragers advanced the
start of foraging of experienced bees. Most inexperienced bees initiated their foraging day after successful foragers had
returned to the hive. The start of foraging by other inexperienced bees was stimulated by high waste-removal activity of nest
mates. By experimentally controlling the entries of foragers (hence external information input) it was shown that very low
levels of external information input had large effect on the departure of experienced foragers. After the return of a single
successful forager, or five foragers together, the rate of forager exits increased dramatically for 15 min. Only the first
and second entry events had large effect; later entries influenced forager exit patterns only slightly. The results show that
Melipona foragers make decisions based upon their own experience and that communication stimulates these foragers if it concerns the
previously visited source. We discuss the organisation of individual foraging in Melipona and Apis mellifera and are led to the conclusion that these species behave very similarly and that an information-integration model (derived
from Fig. 1) could be a starting point for future research on social insect foraging.
Received: 16 April 1997 / Accepted after revision: 30 August 1997 相似文献
968.
Wolf U. Blanckenhorn James W. A. Grant Daphne J. Fairbairn 《Behavioral ecology and sociobiology》1998,42(1):63-70
The monopolization of resources plays an important theoretical role in the literature on competition for food and mates.
We used 12 groups of male water striders (Aquariusremigis) to: (1) test the general prediction that monopolization of both food and mates decreases as the temporal clumping of resources
increases, (2) compare the efficiency of two indices of resource monopolization, coefficient of variation and Q (Ruzzante et al. 1996), and (3) quantitatively assess the resource queue model of Blanckenhorn and Caraco (1992). Each group
of six males competed for both food items and mates released from the upstream end of a laboratory stream. The mean inter-arrival
time for resource units (food or females) was 10 min, with four levels of temporal clumping (variance in inter-arrival time:
0, 25, 50 or 320 min2). As predicted, the monopolization of both food and mates decreased as the temporal clumping of resource arrival increased,
although monopolization was greater for food than for mates. Q detected the difference in monopolization of food and mates, whereas the coefficient of variation did not, because Q is independent of mean resource abundance. The resource queue model successfully predicted monopolization of both resource
types, explaining 89% and 76% of variation in the proportion of food and mates acquired by the six males. The success of the
model suggests that the scaling of handling time to the variance in resource inter-arrival time should play an important role
in any general theory of resource monopolization.
Received: 28 February 1997 / Accepted after revision: 26 September 1997 相似文献
969.
We examined the behavior of individual mature female Mediterranean fruit flies (medflies), Ceratitis capitata (Wiedemann), transferred from a holding cage without fruit to a clean host kumquat fruit already occupied by another medfly
female engaged in ovipositional behavior. A significantly greater proportion of transferred (=test) ovipositionally naive
females initiated ovipositor boring into a fruit in the presence than in the absence of an occupying medfly. Moreover, test
females commenced boring significantly sooner in the presence than in the absence of another medfly. Interestingly, similar
results occurred when a test medfly was transferred to a kumquat occupied by an ovipositing female oriental fruit fly, Bactrocera dorsalis Hendel. In contrast, there was no enhancement of propensity to bore when a test medfly was transferred to a kumquat occupied
by a female Biosteres arisanus (Sonan), a parasitoid attacking medfly eggs. The principal proximate stimulus giving rise to the higher tendency to oviposit
of a test medfly in the presence of an occupying medfly or oriental fruit fly appeared to be wing-waving by the occupant during
an encounter. We consider our findings to be good evidence of socially facilitated ovipositional behavior in ovipositionally
naive medflies, and suggest that such females may benefit from acquisition of a cue demonstrating the acceptability of a host
for oviposition.
Received: 7 July 1997 / Accepted after revision: 30 August 1997 相似文献
970.
Darryl T. Gwynne Winston J. Bailey Amanda Annells 《Behavioral ecology and sociobiology》1998,42(3):157-162
In katydids such as Kawanaphilanartee, a female bias in the operational sex ratio (OSR) results in female competition for mates and male choice of mates. Previous
work showed that the excess of sexually active females occurs when food availability is low, in part because less food increases
the propensity of females to mate as they forage for the large edible spermatophores produced by males. In this study with
K.nartee, a pollen-feeding species, we estimate natural variation in numbers of sexually active males and females by assessing male
calling activity and the propensity of females to respond to experimental calling males. We found an excess of sexually active
males at a site with many flowers and an excess of sexually active females at a site with few flowers about 900 m away. Between-site
differences in gut masses of calling males were consistent with the hypothesis that pollen availability controls OSR. Finally,
at a third site where flowers were at first scarce, we found that the initial excess in sexually active females changed to
an excess of sexually active males after a clump of grass-trees flowered. The mean gut mass of all sampled males from this
site increased after flowering. The large variation in OSR that we document for K. nartee highlights the importance of identifying the appropriate spatial and temporal scales over which OSRs are measured in studies
of factors controlling sexual selection.
Received: 13 May 1997 / Accepted after revision: 27 October 1997 相似文献