Proteomics study of Southern Punjab Pakistani cobra (Naja naja: formerly Naja naja karachiensis) venom

Because of the potential of significant geographic variation in the compositions of snake venoms, much insight can be gained by examining individuals originating from diverse locations, and detailed characterization of any species can only be done by including animals from the entire range. Pakistani N. naja (formerly N. naja karachiensis) venom was decomplexed by applying diverse techniques and found a concoction of proteins (a total of 43 venomous proteins comprising 11 super families) from 6 to 200 kilodaltons in size. Among them three finger toxins (58%), phosphoplipses A₂ (19%), snake venom metalloproteinases (5%), l-amino acid oxidases (5%), helvepryns (3%), vespryns (2%), cobra venom factor (2%), 5′-nucleotidases (2%), venom nerve growth factor (2%), and Kunitz type serine protease inhibitor (2%) were included. It is the first report that this venom possesses significantly higher percentage of three finger toxins belonging to the cytotoxins (32% overall) and α‐neurotoxins (32% overall, predominately long chain neurotoxins, 24% overall) compared to N. naja from other geographic locations. Better understanding of intraspecific variations in venom can improve the development of location-specific anti-venoms. Furthermore, this pool of diverse toxins could potentially be a source of novel drug candidates for the treatment of muscarinic acetylcholine receptors mediated nervous disorders.     

Estimation and comparison of night-time OH levels in the UK urban atmosphere using two di erent analysis methods

Night-time OH levels have been determined for UK urban surface environments using two methods, the decay and steady state approximation methods. Measurement data from the UK National Environmental Technology Centre archive for four urban sites (Bristol, Harwell, London Eltham and Edinburgh) over the time period of 1996 to 2000 have been used in this study. Three reactive alkenes, namely isoprene, 1,3-butadiene and trans-2-pentene were chosen for the calculation of OH levels by the decay method. Hourly measurements of NO, NO2, O3, CO and 20 VOCs were used to determine night-time OH level using the steady state approximation method. Our results showed that the night-time OH levels were in the range of 1 105–1 106 molecules/cm3 at these four urban sites in the UK. The application of a t-test of these analyses indicated that except Bristol, there was no significant di erence between the OH levels found from the decay and steady state approximation methods. Night-time levels of the OH radical appeared to peak in summer and spring time tracking the night-time O3 levels which also passed through a maximum at this time.     

Physicochemical characterization of industrial effluents and their effect on fish survival.

Water samples taken from selected industrial drainages and receiving streams in and around Islamabad, Pakistan, during the summers of 1987 to 1990 showed disturbances in pH and low oxygen levels. Selected metals (Fe, Pb, Zn, Cd, Cu, Ni, Hg) were higher in all effluents than in receiving waters. Static bioassays of the undiluted industrial effluents from three sites caused 100% mortality in carp during the first 24 hrs. Fishes also suffered 30 to 60% mortality when exposed to other undiluted industrial effluents and some mortality when industrial effluents were diluted by 50%.     

The nexus between environmental regulations,economic growth,and environmental sustainability: linking environmental patents to ecological footprint reduction in South Asia

Environmental Science and Pollution Research - Environmental sustainability has become a major concern for policymakers across the globe. In this regard, understanding the factors responsible for...     

Potential health hazard of drinking water in restaurants and tea stalls

Potable and equitable drinking water (DW) is a fundamental human right and essential for human health. This study is conducted to assess the groundwater and jar water quality of the roadside restaurants and tea stalls used for drinking by the local people around the Gazipur City area in Bangladesh. Water samples from 173 restaurants and tea stalls are collected. The physico-chemical and biological parameters are analyzed according to the guidelines and standards. The results illustrate that the color, EC, and Mn of 41%, 80%, and 62% of the samples, respectively, exceed the WHO and Environmental Conservation Rules (ECR) standards. In addition, E. coli and total coliform exceeding the threshold standards are found in 47% and 64% of the water samples, respectively. The contamination of DW by fecal coliforms is confirmed and supported by prior studies, which indicates that the DW supplied in restaurants and tea stalls are unsafe because of the possible presence of pathogens. These may cause potential health hazards to consumers from various water-borne diseases. Poor sanitation, unhygienic practices, and improper disposal of wastewater are responsible for the microbial contamination of DW. So, the authorities in charge of these places should take the right regulatory steps, such as regular sanitation inspections, DW quality monitoring, hand-washing practices, and better sanitation in these places.     

Arsenic uptake and phytotoxicity of T-aman rice (<Emphasis Type="Italic">Oryza sativa</Emphasis> L.) grown in the As-amended soil of Bangladesh

A pot-culture experiment was conducted in open-field conditions with highly cultivated locally transplanted (T) aman rice (Oryza sativa L.) named BR-22 in arsenic (As)-amended soil (0, 1.0, 5.0, 10.0, 20.0, 30.0, 40.0 and 50.0 mg kg⁻¹ As) of Bangladesh to see the effect of As on the growth, yield and metal uptake of rice. Arsenic was applied to soil in the form of sodium arsenate (Na₂HAsO₄). Arsenic affected the plant height, tiller and panicle numbers, grain and straw yield of T-aman rice significantly (P ≤ 0.05). The grain As uptake of T-aman rice was found to increase with increase of As in soil and a high grain As uptake was observed in the treatments of 30–50 mg kg⁻¹ As-containing soil. These levels exceed the food hygiene concentration limit of 1.0 mg kg⁻¹ As. However, the straw As uptake varied significantly (P ≤ 0.05) from a low concentration of As in soil (5 mg kg⁻¹) and the highest uptake was noticed in 20 mg kg⁻¹ As treatment.     

A critical review of the emerging research on the detection and assessment of microplastics pollution in the coastal,marine, and urban Bangladesh

● Coastal and marine regions are the most studied for microplastic pollution. ● Tourism is a major cause of microplastic pollution in coastal regions. ● Sediments contain larger microplastics while fish ingest smaller microplastics. ● Inland lakes, rivers, and freshwater fish are impacted by microplastic pollution. ● Microplastics are found in edible salts, however, presence is less in refined salt. The research on the extent and effects of microplastics pollution in the Global South is only getting started. Bangladesh is a South Asian country with one of the fastest growing economies in the world, however, such exponential economic growth has also increased the pollution threats to its natural and urban environment. In this paper, we reviewed the recent primary research on the assessment of the extent of microplastics pollution in Bangladesh. From the online databases, we developed a compilation of emerging research articles that detected and quantified microplastics in different coastal, marine, and urban environments in Bangladesh. Most of the studies focused on the coastal environment (e.g., beach sediment) and marine fish, while limited data were available for the urban environment. We also discussed the relationship of the type of anthropogenic activities with the observed microplastic pollution. The Cox’s Bazar sea beach in south-east Bangladesh experienced microplastics pollution due to tourism activities, while fishing and other anthropogenic activities led to microplastics pollution in the Bay of Bengal. While microplastics larger than 1 mm were prevalent in the beach sediments, smaller microplastics with size below 0.5 mm were prevalent in marine fish samples. Moreover, the differences in microplastic abundance, size, shape, color, and polymer type found were depended on the sampling sites and relevant anthropogenic activities. It is imperative to identify major sources of microplastics pollution in both natural and urban environment, determine potential environmental and human health effects, and develop mitigating and prevention strategies for reducing microplastics pollution.     

Aerosol size distribution and mass concentration measurements in various cities of Pakistan

During March and April 2010 aerosol inventories from four large cities in Pakistan were assessed in terms of particle size distributions (N), mass (M) concentrations, and particulate matter (PM) concentrations. These M and PM concentrations were obtained for Karachi, Lahore, Rawalpindi, and Peshawar from N concentrations using a native algorithm based on the Grimm model 1.109 dust monitor. The results have confirmed high N, M and PM concentrations in all four cities. They also revealed major contributions to the aerosol concentrations from the re-suspension of road dust, from sea salt aerosols, and from vehicular and industrial emissions. During the study period the 24 hour average PM(10) concentrations for three sites in Karachi were found to be 461 μg m(-3), 270 μg m(-3), and 88 μg m(-3), while the average values for Lahore, Rawalpindi and Peshawar were 198 μg m(-3), 448 μg m(-3), and 540 μg m(-3), respectively. The corresponding 24 hour average PM(2.5) concentrations were 185 μg m(-3), 151 μg m(-3), and 60 μg m(-3) for the three sites in Karachi, and 91 μg m(-3), 140 μg m(-3), and 160 μg m(-3) for Lahore, Rawalpindi and Peshawar, respectively. The low PM(2.5)/PM(10) ratios revealed a high proportion of coarser particles, which are likely to have originated from (a) traffic, (b) other combustion sources, and (c) the re-suspension of road dust. Our calculated 24 hour averaged PM(10) and PM(2.5) concentrations at all sampling points were between 2 and 10 times higher than the maximum PM concentrations recommended by the WHO guidelines. The aerosol samples collected were analyzed for crustal elements (Al, Fe, Si, Mg, Ca) and trace elements (B, Ba, Cr, Cu, K, Na, Mn, Ni, P, Pb, S, Sr, Cd, Ti, Zn and Zr). The averaged concentrations for crustal elements ranged from 1.02 ± 0.76 μg m(-3) for Si at the Sea View location in Karachi to 74.96 ± 7.39 μg m(-3) for Ca in Rawalpindi, and averaged concentrations for trace elements varied from 7.0 ± 0.75 ng m(-3) for B from the SUPARCO location in Karachi to 17.84 ± 0.30 μg m(-3) for Na at the M. A. Jinnah Road location, also in Karachi.     

Assessment of hydrogeochemical characteristics of groundwater in parts of Hindon–Yamuna interfluve region,Baghpat District,Western Uttar Pradesh

The present study has been carried out to assess groundwater quality in parts of Hindon–Yamuna interfluve region of western Uttar Pradesh. Fifty-five groundwater samples were collected from hand pumps in post-monsoon 2005 and pre-monsoon 2006 period, respectively, covering an area of about 1,345 km². Physical and chemical parameters of groundwater such as electrical conductivity, pH, total dissolved solid, Na, K, Ca, Mg, HCO₃, Cl, and SO₄ were determined. Concentration of the chemical constituents in groundwater of the study area varies spatially and temporarily. Interpretation of analytical data of major ion chemistry helps to identify three chemical types of groundwater i.e. ‘mixed’, ‘mixed bicarbonate’ and ‘alkali bicarbonate’ types. The species likely to occur in groundwater of the study area are Ca-HCO₃, Mg-HCO₃, Ca-SO₄, Na-Cl, Na-SO₄, Na-HCO₃, K-Cl, and some other possible species of K, depending on its abundance. The groundwater of the study area comes under the category of moderately hard to very hard, mildly acidic to slightly alkaline in nature. There is anomalously high concentration of major ions, particularly, Na, K, SO₄, and Cl. High SO₄ and K values may be related to anthropogenic influences, rather than through some natural process. Sodium along with Cl may be added to the system through sewage pollution and leachate percolation.     

Improvement of Physico-Mechanical Properties of Photo-Cured Chitosan Films with Ethylene Glycol Dimethacrylate and (2-Hydroxyethyl) Methacrylate

Chitosan, a natural polymer, was prepared by deacetylation of chitin which was obtained from dried prawn shell and was characterized. Thin chitosan film of chitosan was prepared by casting method from 0.2 % chitosan in 2 % acetic acid solution. Five formulations were developed with ethylene glycol dimethacrylate and (2-hydroxyethyl) methacrylate along with photo-initiator, Darocur-1664 (4 %). The chitosan film was soaked in the formulations at different soaking times and irradiated under UV-radiation at different intensities for the improvement of its physical and mechanical properties. The cured chitosan films were then subjected to various mechano-chemical tests like tensile strength, elongation at break, polymer loading, water absorption and gel content. The formulation containing 30 % ethylene glycol dimethacrylate and 66 % (2-hydroxyethyl) methacrylate showed the best performance at the 30th UV pass of UV-radiation for 3 min soaking time.