Evaluation of X,Y, 18, and 13/21 alpha satellite DNA probes for interphase cytogenetic analysis of uncultured amniocytes by fluorescence in situ hybridization

The major aneuploidies diagnosed prenatally involve the autosomes 13, 18, and 21, and sex chromosomes. Fluorescence in situ hybridization (FISH) allows rapid analysis of chromosome copy number in interphase cells. This prospective study evaluated the use of four commercially available centromeric DNA probes (DXZ1, DYZ1, D18Z1, and D13Z1/D21Z1) for direct analysis of uncultured amniocytes. One hundred and sixteen amniotic fluid samples were analysed by FISH and standard cytogenetics. This evaluation demonstrated that FISH with, X, Y, and 18 alpha satellite DNA probes could accurately and rapidly detect aneuploidies involving these chromosomes and could be used in any prenatal clinical laboratory. In contrast, the 13/21 alpha satellite DNA probe hybridizing both chromosomes 13 and 21 was unreliable for prenatal diagnosis in uncultured amniocytes.     

Validation of atmospheric aerosols parallel sampling in a multifold device

In this work, particulate matter was collected using an active sampling system consisting of a PM₁₀ (<10 μm) inlet coupled to a multifold device containing six channels, connected to a vacuum pump. Each channel was equipped with a filter holder fitted with adequately chosen filters. The system was fixed on a metallic structure, which was placed on the roof of the laboratory building, at the Faculty of Sciences, in Lisbon. Sampling took place under flow-controlled conditions. Aerosols were extracted from the filters with water, in defined conditions, and the water-soluble fraction was quantified by ion chromatography (IC) for the determination of inorganic anions (Cl^?, NO₃ ^? and SO₄ ^2?). Equivalent sampling through the various channels was validated. Validation was based on the metrological compatibility of the content results for the various filters. Ion masses are metrologically equivalent when their absolute difference is smaller than the respective expanded uncertainty. When this condition is verified, the studied multifold device produces equivalent samples.     

Adjusting the effect of seasonal variability in the bioassessment of streams

Bioassessment tools should distinguish between the effects of anthropogenic degradation in communities and natural temporal changes. The present study tests the influence of natural seasonal variability on macroinvertebrate stream communities assessed by a predictive model (PORTRIV) and a multimetric index (IPtI) calibrated for spring. The scores of PORTRIV decreased significantly between spring and autumn, and between spring and winter (ca. 37 to 53 %, respectively), while those of IPtI did not change significantly between seasons. For non-reference samples, the results of the predictive model also indicate no significant differences. A correction factor (CF) was calculated to adjust the existing differences in the model assessments between seasons, based on the percentage of variation of reference site scores from spring to autumn and winter. After the application of the CF to the OE50 scores of spring reference samples, the differences were no longer significant. Independent reference validation sites confirmed this tendency. This method has the advantage of avoiding large efforts required for the construction of databases from other seasons and the development of new models to allow the assessment of streams in seasons other than spring. Further tests with models developed in regions with more marked seasonal changes should be done to confirm its wider applicability.     

Increasing land pressure in East Africa: The changing role of cassava and consequences for sustainability of farming systems

Increasing land pressure during the past three to four decades has transformed farming systems in the mid-altitude zone of East Africa. Traditional millet-, cotton-, sugarcane- and/or banana-based farming systems with an important fallow and/or grazing component have evolved into continuously cultivated cassava or cassava/maize-based systems. Within three to four decades, cassava cultivation increased from 1–11 to 16–55% of cropped fields in our six study sites. Declining soil fertility, and not labour or food shortage, was apparently the primary trigger for this transformation. The land use changes have increased nutrient offtakes and reduced nutrient recycling rates. Cassava and maize now account for 50–90% of nutrient removal. Whereas single-season fallows were the most important source of nutrient recycling on cropped fields in the past, currently cassava litterfall and maize stover contribute roughly 70% of nutrient recycling, with 50–70% of N, P and K recycled in cassava litterfall. This may explain why many farmers reason that cassava ‘rests’ the soil. With increasing land use pressure farmers progressively use cassava as an ‘imitation fallow’ throughout their farm. Farmers increasingly target cassava to poor fertility fields characterized by low pH and available P. High cassava intensities are nonetheless maintained on more fertile fields, probably to guarantee regeneration of soil fertility on all fields. Once cassava is targeted to poor fertility soils, farmers have run out of low-input management options and need to intensify management to maintain system productivity. As cassava is now used by more farmers and on a larger acreage than fallowing in the studied farming systems, cassava cropping could perhaps serve as an excellent entry point to strengthen system sustainability.     

Socioeconomic potential of carbon sequestration through agroforestry in the West African Sahel

The recognition of agroforestry as a greenhouse-gas mitigation strategy under the Kyoto Protocol to the United Nations Framework Convention on Climate Change (UNFCCC) offers an opportunity to agroforestry practitioners to benefit from the global Carbon (C) credit market. Our knowledge on this important topic from the semiarid regions such as the West African Sahel (WAS) is, however, very limited. In order to fill this gap, this study was undertaken in the Ségou region of Mali (annual temperature, 29°C; annual rainfall, 300–700 mm in 60 to 90 days), focusing on two improved agroforestry systems (live fence and fodder bank) along with traditional parkland agroforestry systems of the region. A cost–benefit analysis was conducted to assess the economic profitability and risks associated with the systems considering them as 25-year projects and their potential for participation in C credit market. The traditional systems had high C stock in their biomass and soil, but little potential for sequestering additional C; on the other hand, the improved systems had low C stock, but high sequestration potential. For the standard size live fence (291 m) and fodder bank (0.25 ha) projects, the estimated net present values (NPV) were $ 96.0 and $158.8 without C credit sale, and $109.9 and $179.3 with C sale, respectively. From the C sale perspective, live fence seemed less risky and more profitable than fodder bank. Carbon credit sale is likely to contribute to economic development of the subsistence farmers in the WAS.

Runoff and sediment losses from 27 upland catchments in Southeast Asia: Impact of rapid land use changes and conservation practices

Rapid changes in upland farming systems in Southeast Asia generated predominantly by increased population pressure and ‘market forces’ have resulted in widespread land degradation that has been well documented at the plot scale. Yet, the links between agricultural activities in the uplands and downstream off-site effects remain largely unknown because of the difficulties in transferring results from plots to a larger scale. Many authors have thus pointed out the need for long-term catchment studies. The objective of this paper is to summarize the results obtained by the Management of Soil Erosion Consortium (MSEC) over the last 5 years from 27 catchments in five countries (Indonesia, Laos, Philippines, Thailand, and Vietnam). The purpose of the study was to assess the impacts of cultivation practices on annual runoff and erosion rates. Initial surveys in each catchment included topography, soils and land use. Monitoring included climatic, hydrologic and erosion (total sediment yield including bed load and suspended sediment load) data, land use and crop yields, and farmers’ income. In addition, new land management options were introduced through consultations with farmers and evaluated in terms of runoff and erosion. These included tree plantations, fruit trees, improved fallow with legumes, maize intercropped with legumes, planted fodder, native grass strips and agro-ecological practices (direct sowing and mulch-based conservation agriculture). Regressions analyses showed that runoff during the rainy season, and normalized runoff flow coefficient based on erosive rainfall during the rainy season (rainfall with intensity exceeding 25 mm h⁻¹) increase with the percentage of the catchment covered by maize. Both variables decrease with increasing soil depth, standard deviation of catchment slope (that reflects terrain roughness), and the percentages of the catchment covered by fallow (regular and improved), tree plantations and planted fodder. The best predictors of sediment yield were the surface percentages of maize, Job's tears, cassava and footpaths. The main conclusions generated from this study were: (i) soil erosion is predominantly influenced by land use rather than environmental characteristics not only at the plot scale but also at the catchment scale; (ii) slash-and-burn shifting cultivation with sufficiently long rotations (1 year of cultivation, 8 years of fallow) is too often unjustly blamed for degradation; (iii) in its place, continuous cropping of maize and cassava promotes high rates of soil erosion at the catchment scale; (iv) conservation technologies are efficient in reducing runoff and total sediment yield at the catchment scale; (v) the adoption of improved soil management technologies by upland farmers is not a function of the degree of intensification of their farming system and/or of their incomes. The results suggest that if expansion of maize and cassava into already degraded upland systems were to occur due to increased demand for biofuels, there is a risk of higher runoff and sediment generation. A failure to adopt appropriate land use management strategies will result in further rapid resource degradation with negative impacts to downstream communities.