Exploring the role of chitinolytic enzymes in the sea fan coral, <Emphasis Type="Italic">Gorgonia ventalina</Emphasis>

Chitinases are involved in defense against chitinaceous pathogens in both invertebrates and vertebrates. This study investigated whether sea fan corals, Gorgonia ventalina (Linnaeus) collected from the Florida Keys between the summer of 2002 and the summer of 2005 contain chitinases, and whether these enzymes could serve an analogous protective role against the fungal pathogen, Aspergillus sydowii (Thom et Church). Crude extracts of healthy sea fans contained detectible levels of exochitinase activity in an in vitro microplate assay using fluorogenic substrates. The exochitinase levels decreased upon injury, agitation, or manipulation of the tissue. A concurrent, transient increase of exochitinase in the surrounding water suggests that sea fans release chitinases as a response to these stresses. By contrast, endochitinase was detected in only 2 of 15 sea fans (13%), suggesting a high degree of variation for this enzyme. Sea fan chitinase-containing seawater and anion exchange chromatography fractions were both active against A. sydowii in an absorbance-based antifungal assay. The presence of chitinases in sea fan extracts, their release into the surrounding water upon stress, and their activity against A. sydowii suggests that further study of these enzymes in coral stress responses is warranted.     

Managing Alaska’s National Parks in an era of uncertainty: an evaluation of scenario planning workshops

Regional Environmental Change - Scenario planning is a flexible tool used to assess a broad range of plausible, relevant, divergent, and challenging futures, for short-term responses and long-term...     

The Lost Creek Fire: managing social relations under disaster conditions

This paper examines some of the social processes associated with disaster conditions. Utilising an asset‐based perspective of community capacity, it focuses on four types of normative systems to interpret the ability of communities to manage disasters through market‐, bureaucratic‐, associative‐, and communal‐based norms. Drawing on experience of a wildfire in the Crowsnest Pass region of southwest Alberta, Canada, in 2003, the tensions and compatibilities among these normative systems are evaluated through interviews with 30 community leaders. The results confirm the contributions of all types of social capital to resiliency, the necessity for rapid use of place‐based knowledge, and the importance of communication among all types and levels of agents. In addition, they point to the value of identifying and managing potential conflicts among the normative systems as a means to maximising their contributions. The integration of local networks and groups into the more general disaster response minimised the impacts on health and property.     

Surrogate Measures for Providing High Frequency Estimates of Total Suspended Solids and Total Phosphorus Concentrations1

Spackman Jones, Amber, David K. Stevens, Jeffery S. Horsburgh, and Nancy O. Mesner, 2010. Surrogate Measures for Providing High Frequency Estimates of Total Suspended Solids and Total Phosphorus Concentrations. Journal of the American Water Resources Association (JAWRA) 1‐15. DOI: 10.1111/j.1752‐1688.2010.00505.x Abstract: Surrogate measures like turbidity, which can be observed with high frequency in situ, have potential for generating high frequency estimates of total suspended solids (TSS) and total phosphorus (TP) concentrations. In the semiarid, snowmelt‐driven, and irrigation‐regulated Little Bear River watershed of northern Utah, high frequency in situ water quality measurements were recorded in conjunction with periodic chemistry sampling. Site‐specific relationships were developed using turbidity as a surrogate for TP and TSS at two monitoring locations. Methods are presented for employing censored data and for investigating categorical explanatory variables (e.g., hydrologic conditions). Turbidity was a significant explanatory variable for TP and TSS at both sites, which differ in hydrologic and water quality characteristics. The relationship between turbidity and TP was stronger at the upper watershed site where TP is predominantly particulate. At both sites, the relationships between turbidity and TP varied between spring snowmelt and base flow conditions while the relationships between TSS and turbidity were consistent across hydrological conditions. This approach enables the calculation of high frequency time series of TP and TSS concentrations previously unavailable using traditional monitoring approaches. These methods have broad application for situations that require accurate characterization of fluxes of these constituents over a range of hydrologic conditions.     

The Identification of Atmospheric Nitric Oxide by a Spectroscopic Scan

Lines of the 1-0 band of NO near 5.3 jum have been observed in spectra of approximately 500 m of ground-level air at Columbus, Ohio, on several occasions between October 1970 and January 1971. The estimated NO abundances lie between 0.0 and 0.08 ppm with uncertainties of ±0.01 ppm.     

Interaction between schools of fish and mysids

Summary A long-term daily census of 20 small patch reefs along the backreef of Tague Bay barrier reef, U.S. Virgin Islands, established that recently recruited postlarval French grunts, Haemulon flavolineatum, form mixed schools with at least two species of mysids (genus Mysidium). The formation of these taxonomically unrelated animals into socially interacting groups probably occurs because of the striking resemblance of postlarval grunts to mysids. Over a period of 5 d and as the postlarval grunts grow, these mixed associations break down, only to reform every 14–15 d as new postlarval grunts periodically recruit from the plankton. The fleeting but persistent formation of these mixedspecies schools is believed to initially provide protection to the grunts and, as the grunts grow, limited protection to the mysids. In additions, the grunts benefit trophically from the associations because they prey on the mysids.