Evaluation of the possible role of five enzymes in the metabolism of IBA in mustard aphid, Lipaphis erysimi (Kalt.)

The enzymatic activity of five enzymes viz. Glutathione S-transferases, Esterases, NADH dehydrogenase, NADH oxidase and Glutathione reductase were assessed under the influence of Indole butyric acid (IBA) (400 ppm) in the nymphs (48-52h old) of mustard aphid, Lipaphis erysimi fed on radish plants treated for 13, 25 and 37h. The activity of Glutathione S-transferases, Esterases and NADH dehydrogenase increased compared to that found in the control of the same age group of nymphs and it was concluded that these enzymes might be involved in the metabolism of IBA. The other two enzymes, NADH oxidase and Glutathione reductase showed no significant increase in their activity compared to that in the control of the same age group. It was hypothesized that the latter enzymes do not play any significant role in the metabolism of IBA.     

Effect of arsenic contamination on microbial biomass and its activities in arsenic contaminated soils of Gangetic West Bengal, India

A study was conducted to see the effect of arsenic contamination on soil quality indicators, viz., microbial biomass, soil respiration, fluorescein diacetate and dehydrogenase (DHG) activity in arsenic contaminated soils of West Bengal. All the parameters were significantly and negatively correlated with all the form of arsenic (bioavailable and total) but the microbial metabolic quotient was significantly and positively correlated with all forms of arsenic, indicating arsenic induced stress to the soil microbial community. This may be due to part of the microbial biomass, which is located in the inner parts of the micro-aggregates of soil, which is affected by arsenic accumulates present in soil particles. Linear regression analysis revealed that the bioavailable arsenic exerted greater inhibitory effect on the soil microbial population than the total arsenic content of soils. Water-soluble arsenic showed more inhibitory effect than NaHCO(3) extractable form, in their association with biological properties of the contaminated soils. Water-soluble form of arsenic was much more toxic than insoluble forms. This signified that with increase in bioavailability, the arsenic exerted more inhibitory effect on these parameters. It is thus suggested that the microbial biomass, fluorescein diacetate and dehydrogenase activity alone and expressed on a soil organic matter basis along with the soil respiration parameters can be helpful in assessing the effects of arsenic on the size and activity of microbial biomass in soils.     

Application of chemical transformation induced fracture for cutting of superhard materials

A hybrid CO₂ laser/waterjet machining system is used to cut superhard materials – polycrystalline diamond (PCD) and polycrystalline cubic boron nitride (pCBN) through a “score and snap” mechanism – laser heating leads to localized damage and chemical transformation of surface layers, and subsequently, stress fields developed due to constrained expansion of transformed material and waterjet quenching act on the laser-made “score” to propagate crack through the thickness. During the localized laser heating and subsequent waterjet quenching, PCD and pCBN materials near the top surface underwent chemical transitions. Build up of tensile stresses in the surrounding material resulted due to (i) volumetric expansion of transformed material creating an asperity/wedge effect and (ii) thermal gradients associated with rapid quenching. These tensile stresses in the surface layers lead to crack formation and caused separation of PCD and pCBN wafers along the laser path.     

Biochemical responses,growth and reproduction of earthworm in low density polyethylene (LDPE)

There is an unprecedented production of plastic that is accelerating its disposal while affecting the fitness of the terrestrial as well as the aquatic environment. The term microplastics refers to plastic fragments that are less than 5 mm in size and are widely distributed in the environment. Therefore, the present study intends to explore the biological response of earthworms (Eisenia fetida) toward different concentrations of low-density polyethylene. E. fetida treated with low-density polyethylene concentration (Control), 250 mg kg⁻¹, 1000 mg kg⁻¹, 6000 mg kg⁻¹, 12,000 mg kg^−1, and 25,000 mg kg⁻¹. The above ratios were thoroughly mixed with 1kg of artificial soil and tested for growth, reproduction (cocoons and hatchling count), and enzymatic activities namely superoxide dismutase, guaiacol peroxidase, glutathione-S-transferase, and glutathione reductase and molecular docking studies. No mortality was observed during the exposure period at any concentrations. On the 28th day, when compared to the control the highest decrease in body weight of earthworms was observed in 25,000 mg (28.4%) followed by 12,000 mg (12.2%) and 6000 mg (3.4%). The cocoon and hatchlings significantly declined as the dose of microplastics increases. Enzymatic activity such as SOD and POD showed declined trend as the dose increased, while GST and GR increased with an increase in microplastic concentrations on 28th day. Furthermore, molecular docking showed that LDPE can modulate the activity of all four enzymes significantly.     

Quantification of Extinction Risk: IUCN's System for Classifying Threatened Species

Abstract: The International Union for Conservation of Nature (IUCN) Red List of Threatened Species was increasingly used during the 1980s to assess the conservation status of species for policy and planning purposes. This use stimulated the development of a new set of quantitative criteria for listing species in the categories of threat: critically endangered, endangered, and vulnerable. These criteria, which were intended to be applicable to all species except microorganisms, were part of a broader system for classifying threatened species and were fully implemented by IUCN in 2000. The system and the criteria have been widely used by conservation practitioners and scientists and now underpin one indicator being used to assess the Convention on Biological Diversity 2010 biodiversity target. We describe the process and the technical background to the IUCN Red List system. The criteria refer to fundamental biological processes underlying population decline and extinction. But given major differences between species, the threatening processes affecting them, and the paucity of knowledge relating to most species, the IUCN system had to be both broad and flexible to be applicable to the majority of described species. The system was designed to measure the symptoms of extinction risk, and uses 5 independent criteria relating to aspects of population loss and decline of range size. A species is assigned to a threat category if it meets the quantitative threshold for at least one criterion. The criteria and the accompanying rules and guidelines used by IUCN are intended to increase the consistency, transparency, and validity of its categorization system, but it necessitates some compromises that affect the applicability of the system and the species lists that result. In particular, choices were made over the assessment of uncertainty, poorly known species, depleted species, population decline, restricted ranges, and rarity; all of these affect the way red lists should be viewed and used. Processes related to priority setting and the development of national red lists need to take account of some assumptions in the formulation of the criteria.     

Appraisal of stormwater-induced runoff quality influenced by site-specific land use patterns in the south-eastern region of Bangladesh

Environmental Science and Pollution Research - Urban stormwater runoff is considered as one of the major contributors to nonpoint source that contributes to the pollution of all water resources in...     

Commensal in conflict: Livestock depredation patterns by free-ranging domestic dogs in the Upper Spiti Landscape,Himachal Pradesh,India

In human-populated landscapes worldwide, domestic dogs (Canis lupus familiaris) are the most abundant terrestrial carnivore. Although dogs have been used for the protection of livestock from wild carnivores, they have also been implicated as predators of livestock. We used a combination of methods (field surveys, interview surveys, and data from secondary sources) to examine the patterns and factors driving livestock depredation by free-ranging dogs, as well as economic losses to local communities in a Trans-Himalayan agro-pastoralist landscape in India. Our results show that livestock abundance was a better predictor of depredation in the villages than local dog abundance. Dogs mainly killed small-bodied livestock and sheep were the most selected prey. Dogs were responsible for the majority of livestock losses, with losses being comparable to that by snow leopards. This high level of conflict may disrupt community benefits from conservation programs and potentially undermine the conservation efforts in the region through a range of cascading effects.     

Hydrodynamics and turbulence in emergent and sparsely vegetated open channel flow

This present study reports the results of an experimental study characterizing thorough variation of turbulent hydrodynamics and flow distribution in emergent and sparsely vegetated open channel flow. An emergent and rigid sparse vegetation patch with regular spacing between stems along the flow and transverse directions was fixed in the central region of the cross-section of open channel. Experiments were conducted in subcritical flow conditions and velocity measurements were obtained with an acoustic Doppler Velocimetry system. Large variations of the turbulence intensities, Reynolds shear stress, turbulent kinetic energy and vortical motions are found in and around the vegetation patch. At any cross-section through the interior of the vegetation patch, streamwise velocity decreases with increase in streamwise length and the velocity profiles converge from the log-law to a linear profile with increasing slope. Time-averaged lateral and vertical velocities inside the vegetation patch increase with increasing streamwise distance and converge from negative values to positive values. Turbulence intensities interior of the sparse vegetation patch are more than those of without the vegetation patch. Similar to the trend of streamwise velocity profiles inside the vegetation, turbulence intensities and longitudinal-normal Reynolds shear stress profile decreases with streamwise direction. In the interior of the vegetation patch and downstream of the trailing edge, turbulent kinetic energy profiles are exhibiting irregular fluctuations and the maximum values are occurring in the outer layer. Analysis of flow distribution confirms sparse vegetation patch is inducing a serpentine flow pattern in its vicinity. At the leading edge, flow is rushing towards the right hand sidewall, and at the trailing edge, flow is turning to the left hand sidewall. In between the leading and trailing edges, the streamlines are following a zig-zag fashion at varied degree along the streamwise and lateral directions. Immediate upstream of the leading edge and in the interior of the vegetation patch, vortex motion is clearly visible and the vortices are stretched along the width of the channel with streamwise direction.     

Intensive cattle grazing affects pasture litter-fall: an unrecognized nitrous oxide source

The rationale for this study came from observing grazing dairy cattle dropping freshly harvested plant material onto the soil surface, hereafter called litter-fall. The Intergovernmental Panel on Climate Change (IPCC) guidelines include NO emissions during pasture renewal but do not consider NO emissions that may result from litter-fall. The objectives of this study were to determine litter-fall rates and to assess indicative NO emission factors (EFs) for the dominant pasture species (perennial ryegrass [ L.] and white clover [ L.]). Herbage was vacuumed from intensively managed dairy pastures before and after 30 different grazing events when cows (84 cows ha) grazed for 24 h according to a rotational system; the interval between grazing events ranged from 21 to 30 d. A laboratory incubation study was performed to assess potential EF values for the pasture species at two soil moisture contents. Finely ground pasture material was incubated under controlled laboratory conditions with soil, and the NO emissions were measured until rates returned to control levels. On average, pre- and postgrazing dry matter yields per grazing event were 2516 ± 636 and 1167 ± 265 kg DM ha (±SD), respectively. Pregrazing litter was absent, whereas postgrazing fresh and senesced litter-fall rates were 53 ± 24 and 19 ± 18 kg DM ha, respectively. Annually, the rotational grazing system resulted in 12 grazing events where fresh litter-fall equaed to 16 kg N ha yr to the soil. Emission factors in the laboratory experiment indicated that the EF for perennial ryegrass and white clover ranged from 0.7 to 3.1%. If such EF values should also occur under field conditions, then we estimate that litter-fall induces an NO emission rate of 0.3 kg NO ha yr. Litter-fall as a source of NO in grazed pastures requires further assessment.