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101.
Paolo Gasparini Giuseppe Novelli Anna Savoia Bruno Dallapiccola Pier Franco Pignatti 《黑龙江环境通报》1989,9(5):349-355
Eight pregnancies at risk for cystic fibrosis have been monitored by first-trimester prenatal diagnosis with DNA amplification analysis. The polymerase chain reaction (PCR) was used in all cases to amplify the region detected by KM 19. In two cases, the region detected by CS·7, another DNA probe tightly linked to the CF locus, was also examined. The results of the PCR determinations were confirmed using the Southern blotting procedure, by segregation analysis of restriction fragment length polymorphisms (RFLPs) relative to XV-2c, J3·11, metH, metD, and KM19 probes. 相似文献
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106.
Paolo Viotti Paolo Roberto Di Palma Federico Aulenta Antonella Luciano Giuseppe Mancini Marco Petrangeli Papini 《Environmental science and pollution research international》2014,21(2):1514-1527
In this paper, a numerical model is presented that is capable of describing the complex set of biochemical processes that occur in chlorinated aliphatic hydrocarbon (CAH)-contaminated groundwater when an exogenous electron donor is added. The reactive pattern is based on the degradation pathways of both chlorinated ethanes and ethenes, and it includes electron donor production (H2 and acetate) from the fermentation of an organic substrate as well as rate-limiting processes related to electron acceptor competition. Coupling of the kinetic model to a convection–dispersion module is described. The calibration phase was carried out using data obtained at a real CAH-contaminated site in the north of Italy. Model simulations of different application scenarios are presented to draw general conclusions on the effectiveness of reductive dechlorination (RD) as a possible cleanup strategy. Early outcomes indicate that cleanup targets can only be achieved if source longevity is reduced. Therefore, metabolic RD is expected to produce beneficial effects because it is known to induce bioenhanced degradation and transformation of CAHs. 相似文献
107.
Paolo Montuori Teresa Cirillo Evelina Fasano Antonio Nardone Francesco Esposito Maria Triassi 《Environmental science and pollution research international》2014,21(7):5023-5035
The Sarno River is nicknamed “the most polluted river in Europe”. The main goal of this study is to enhance our knowledge on the Sarno River water and sediment quality and on its environmental impact on the gulf of Naples (Tyrrhenian Sea, Central Mediterranean Sea) in order to become a useful assessment tool for the regional administrations. For these reasons, 32 selected polychlorinated biphenyls (PCBs) and aldrin, α-BHC, β-BHC, δ-BHC, γ-BHC (lindane), 4,4′-DDD, 4,4′-DDE, 4,4′-DDT, dieldrin, endosulfan I, endosulfan II, endosulfan sulphate, endrin, endrin aldehyde, heptachlor, heptachlor epoxide (isomer B) and methoxychlor were determined in the water dissolved phase (DP), suspended particulate matter (SPM) and sediments. Total concentrations of PCBs ranged from 1.4 to 24.9 ng L?1 in water (sum of DP and SPM) and from 1.01 to 42.54 ng g?1 in sediment samples. The concentrations of total organochlorine pesticides (OCPs) obtained in water (sum of DP and SPM) ranged from 0.54 to 7.32 ng L?1 and from 0.08 to 5.99 ng g?1 in sediment samples. Contaminant discharges of PCBs and OCPs into the sea were calculated in about 1,247 g day?1 (948 g day?1 of PCBs and 326 g day?1 of OCPs), showing that this river should account as one of the main contribution sources of PCBs and OCPs to the Tyrrhenian Sea. 相似文献
108.
Paolo F. Ricci Louis Anthony Cox Jr. John P. Dwyer 《Journal of the Air & Waste Management Association (1995)》2013,63(8):1046-1053
The acceptability of cancer risk requires consideration of factors that extend beyond mere numerical representations, such as either individual lifetime risk in excess of background and excess incidence. Recently, use of these numbers has been tempered by the addition of qualitative weights-of-evidence that describe the degree of support provided by animal and epidemiologic results. Nevertheless, many other factors, most of which are not quantitative, require incorporation but remain neglected by the analyst eager to use quantitative results. In this paper we show that simple risk measures are often fraught with problems. Moreover, these measures do not incorporate the very essence of acceptability, which includes notions of responsibility, accountability, equity, and procedural legitimacy, among others. We link the process of risk assessment to those legal and regulatory standards that shape it. These standards are among the principal means to resolve risk-related disputes and to enhance the balancing of competing interests when science and law meet on uncertain and often conjectural ground. We conclude the paper with a proposal for the portfolio approach to manage cancer risks and to deal with uncertain scientific information. This approach leads to the concept of "provisional acceptability," which reflects the choices available to the decisionmaker, and the trade-offs inherent to such choices. 相似文献
109.
Fabio Gosetti Michela Bottaro Valentina Gianotti Eleonora Mazzucco Paolo Frascarolo Davide Zampieri Caterina Oliveri Aldo Viarengo Maria Carla Gennaro 《Environmental pollution (Barking, Essex : 1987)》2010,158(2):592-146
This paper studies the degradation reactions that 4-chloroaniline can naturally undergo in waters for the action of sun light. 10.00 mg L−1 4-chloroaniline aqueous solution, without any addition of organic solvent, are undergone to photoirradiation under conditions that simulate sun light. The degradation pathway, followed by HPLC-DAD-MS/MS methods, is complex since the pollutant gives rise to many photoproducts: the predominant species are characterized by m/z values of 217 (P5) and 218 (P6) and are compatible with dimeric structures of 4-chloroaniline. Vibrio fischeri tests indicate that the photoproducts of 4-chloroaniline are characterized by a toxicity level significantly greater than the precursor. 相似文献
110.
Using precautionary principles when facing incomplete facts and causal conjectures raises the possibility of a Faustian bargain. This paper applies systems dynamics based on previously unavailable data to show how well intended precautionary policies for promoting food safety may backfire unless they are informed by quantitative cause-and-effect models of how animal antibiotics affect animal and human health. We focus on European Union and United States formulations of regulatory precaution and then analyze zoonotic infections in terms of the consequences of relying on political will to justify precautionary bans. We do not attempt a political analysis of these issues; rather, we conduct a regulatory analysis of precautionary legal requirements and use Quantitative Risk Assessment (QRA) to assess a set of policy outcomes. Thirty-seven years ago, the Joint Committee on the Use of Antibiotics in Animal Husbandry and Veterinary Medicine (the Swann Report) warned that uncontrolled use of similar antibiotics in humans and food animals could promote the emergence of resistant strains of foodborne bacteria that could endanger human health. Since then, many countries have either banned or restricted antibiotics as feed additives for promoting animal growth. Others, including the United States, have relied on prudent use guidelines and programs that reduce total microbial loads, rather than focusing exclusively on antibiotic-resistant bacteria. In retrospect, the regulatory strategy of banning or restricting animal antibiotic uses has had limited success: it has been followed in many cases by deteriorating animal health and increases in human illnesses and resistance rates. Conversely, a combination of continued prudent use of antibiotics to prevent and control animal infections, together with HACCP and other improvements, has been followed by large improvements in the microbial safety of chickens and other food animals in the United States, leaving both animals and people better off now than they were decades ago. A quantitative risk assessment model of microbiological risks (Campylobacter because of data availability) suggests that these outcomes may be more than coincidental: prudent use of animal antibiotics may actually improve human health, while bans on animal antibiotics, intended to be precautionary, inadvertently may harm human health. 相似文献