Comparison of extraction methods for polycyclic aromatic hydrocarbon determination in sediments

Different sample preparation methods for the determination of polycyclic aromatic hydrocarbons (PAHs) in lake and river sediments were compared and evaluated. Freeze‐dried sediment materials were extracted using Soxhlet extraction, ultrasonic agitation and digestion with various solvents and solvent mixtures. Similarly, clean‐up methods using various types of sorbents for separation of PAHs tested.

The following method gives the best results for the determination of PAHs: freeze‐dried sediment samples were extracted using Soxhlet extractor with dichloromethane (6 hr, dark), extracts were cleanup using Silica gel and a mixture of n‐hexa‐ne‐dichloromethane was used for the elution of PAHs.

This method was used for the determination of 7 selected PAHs in sediment samples from Finnish lakes and Czech streams.     

Safety compliance climate concerning risk assessment and preventive measures in EU legislation: A Finnish survey

The aim of the present study is to explore the process dynamics of the safety compliance climate and work relationships, including their antecedents and consequences. After investigating the many dimensions of safety compliance climate and of work relationships, the study concludes that a multi-component measure is needed to fully assess all of their dimensions. With respect to the impacting positively on safety compliance legislation, the present study found the following factors: “contribution measures”, “concrete preventive measures” and “risk assessment process”. For risk assessment factors on safety compliance the study identified “planning, guidelines, policy, management”, “prioritization of proactive measures”, “results in cooperation and information” and “active use of risks assessment document”. In a regression model for the workers, both risk assessment process and contribution measures had a statistically highly significant (p < 0.001) effect on concrete preventive measures. In the regression analysis of the employers risk assessment process had a statistically significant (p < 0.01) effect and contribution measures had a statistically highly significant (p < 0.001) effect on concrete preventive measures.     

Lack of population structure in the fiddler crab Uca annulipes along an East African latitudinal gradient: genetic and morphometric evidence

Phylogeographic patterns in Uca annulipes sampled from 30 locations across an East African latitudinal gradient were investigated using mitochondrial DNA cytochrome oxidase I sequences and analysed together with patterns of morphometric differentiation. Four hundred and four specimens along the east African coast were sampled, and 18 haplotypes were encountered. Haplotype and nucleotide diversity values were very low and the phylogenetic analysis did not reveal any clear phylogeographic structure. Furthermore, the analysis of molecular variance and pairwise Φ _ST values showed no significant spatial population differentiation. Mismatch analyses and tests of neutrality supported the hypothesis that this species has undergone a fairly recent demographic expansion. Our results, therefore, failed to demonstrate significant geographical structure in the pattern of genetic variation, indicating that populations of U. annulipes are capable of extensive gene flow among mangroves along the coast. The genetic structure of this species could be panmictic due to a high amount of gene flow along the geographical gradient in study during the planktonic larval phase, when larvae are carried along stream by the dominant currents. Moreover, the morphometric analysis performed did not reveal differences of shape differentiation according to a geographical pattern, although significant differences among the sampling areas were found.     

Modelling the effects of extreme events on the dynamics of the amphipod Corophium orientale

The brackish water amphipod Corophium orientale is the dominant macroinvertebrate species in the upper Mira estuary, a small mesotidal system located in the southwest coast of Portugal. As climate changes will increase the frequency and intensity of extreme events such as floods and droughts, these will have a negative effect on benthic estuarine invertebrates, namely C. orientale. In order to understand the effects of these events on C. orientale, a dynamic model, based on published information and calibrated with field data, was developed and different scenarios were tested.For model construction, the annual development of three cohorts of C. orientale, their growth rates, and the establishment of the timing of each cohort rise and extinction are introduced. This structure can be repeated indefinitely, for years, and few parameters are required. The model simulations highlight the need for refuge areas that enable a fast recovery of the amphipod population after an extreme event and the recolozination of the affected areas.     

The need to look beyond the production and provision of relief seed: experiences from southern Sudan

Free distribution of seeds in selected areas of southern Sudan has been widespread as a way of increasing food security. Field research in areas targeted for seed relief found that farmer seed systems continue to meet the crop and varietal needs of farmers even following the 1998 famine. Donor investments in seed multiplication of improved sorghum have not been sustained due to a lack of effective demand for the improved seed beyond that created by the relief agencies. The article argues that rather than imposing outside solutions, whether through seed provisioning or seed production enterprises, greater attention needs to be given to building on the strengths of existing farmer systems and designing interventions to alleviate the weaknesses. The case is made to support dynamically the process of farmer experimentation through the informed introduction of new crops and varieties that can potentially reinforce the strength and diversity of local cropping systems.     

Hypomandibular faciocranial dysostosis in consanguineous parents revealed by ultrasound prenatal diagnosis

We report here the fourth case of hypomandibular faciocranial dysostosis (HFD). The diagnosis was made at birth on the association of severe retrognathia, microstomia, severe hypoglossia with glossoptosis, persistent buccopharyngeal membrane, median cleft palate, bifid uvula, down-slanting palpebral fissures, short nose with anteverted nares, laryngeal hypoplasia, and low-set ears. A severe microstomia and micrognathia were detected by ultrasound at 31 weeks of gestation. Interestingly, even though the present case exhibits many facial dysmorphic features characteristic of HFD, craniosynostosis was absent. This report suggests that craniosynostosis is not mandatory for the diagnosis of this condition. Furthermore, we present a new argument for an autosomal recessive mode of inheritance for HFD. Copyright © 2002 John Wiley & Sons, Ltd.     

Assessment of crown condition in eucalypt vegetation by remotely sensed optical indices

Leaf and crown damage and discoloration characteristics are important variables when defining the health of eucalypt tree species and have been used as key indicators of environmental quality. These indicators can vary significantly over a few hectares, especially in mixed-species forests, making field-based environmental surveillance of crown condition an extremely expensive and logistically impractical task. Reflectance in narrow spectral wavelengths obtained from a field-based spectroradiometer and a Compact Airborne Spectrographic Imager 2 (CASI-2) were collected over eucalypt vegetation of varying condition in southeastern Australia and compared with leaf- and crown-based attributes including percent red foliage discoloration, percent leaf damage, and crown density and crown foliage condition. Of the leaf attributes sampled, percent leaf damage was well correlated with a red-green spectral index (r = 0.68, p < 0.01), and percent red discoloration was well correlated with the slope of the red-edge for selected species (r = 0.89, p < 0.001). Within-tree crown density was well correlated with the slope of the red-edge (r = 0.77, p < 0.001) and a previously published index of plant stress with crown foliage condition (r = 0.88, p < 0.01) for selected species. Despite evidence of strong interspecific variability, a set of narrow spectral wavelengths in the visible and near-infrared regions of the spectrum have been identified that will be useful in the development of forest ecosystem environmental quality indicators.     

Heavy metal accumulation by the halophyte species Mediterranean saltbush

To identify Cd- and Zn-accumulating plants exhibiting a high growth rate, seeds from the halophyte species Mediterranean saltbush (Atriplex halimus L.) were collected on a heavy-metal-contaminated site in southeastern Spain (Llano del Beal, Cartagena). Seedlings from this ecotype were exposed for 3 wk to 0.1 mM Cd or Zn in a nutrient solution in a fully controlled environment. All plants remained alive and no significant growth inhibition was recorded until the end of the experiment. Mean Cd and Zn accumulation in aerial parts was 830 and 440 mg kg(-1), respectively, and the rate of metal translocation even increased with the duration of stress exposure. Resistance to heavy metals in this species may be partly linked to precipitation of Cd in oxalate crystals in the stems. A Cd-induced decrease in glutathione concentration also suggests that phytochelatins overproduction may occur in these conditions. We conclude that Mediterranean saltbush, which is able to produce up to 5 Mg dry matter ha(-1) yr(-1), may be an effective species for phytoextraction and should be tested for this purpose in field conditions.     

Antecedents of fatigue, close calls, and crashes among commercial motor-vehicle drivers

PROBLEM: Minimizing driver fatigue among commercial motor-vehicle (CMV) drivers is a major safety issue in the United States. This study examines the effects of potentially fatigue-inducing factors inherent in truck driving work and company safety management in explaining: (a) drivers driving while fatigued, (b) the frequency of close calls due to fatigue, and (c) actual crashes among CMV drivers. METHOD: Data for this study are derived from a survey of CMV drivers in 116 trucking firms, with all data being driver-reported. The relative roles of fatigue-inducing factors and safety management practices in explaining variation in fatigue, close calls, and crashes are reported, along with the roles of fatigue in affecting close calls and crashes via hierarchical regression. RESULTS: Findings indicated that fatigue-inducing factors inherent in driving work and safety practices accounted for appreciable variation in driving fatigued (R(2) =.42) and close calls (R(2) =.35), but not crash involvement. Driving while fatigued also accounted for incremental increases in the amount of variation in close calls, after consideration of inherent factors and safety practices. IMPACT ON INDUSTRY: Findings indicate that safety practices (e.g., establishment of a strong safety culture, dispatcher scheduling practices, company assistance with fatiguing behaviors such as loading and unloading) have considerable potential to offset fatigue-inducing factors associated with truck driving work.