Characteristics of organic acid profiles in 16 species of wild growing edible mushrooms

The aim of study was to determine 9 organic acids in nine aboveground and seven wood-growing wild edible mushroom species originated from an area under the direct influence of a busy trunk road in Poland. The organic acids in the extracts of samples were identified by reversed-phase column liquid chromatography (RPLC). The presented results show that all the mushroom species were characterized by high variation in the profile and content of the analyzed acids. Each of mushroom species contained oxalic acid, the profiles of the other acids strictly depended on mushroom species. Among aboveground species, the highest total content of organic acids was found in Lepista gilva (267.5 ± 26.6 mg g^?1 dry weight (DW)), while Laccaria amethystina was characterized by the lowest content (37.7 ± 6.5 mg g^?1 DW). Within wood-growing species, the highest content of organic acids was determined in Flammulina velutipes (171.9 ± 26.7 mg g^?1 DW), whereas the lowest content (34.2 ± 2.9 mg g^?1 DW) of the studied acids was observed in Grifola frondosa.     

Spatial and seasonal variability of pore water phosphorus concentration in shallow Lake Swarzędzkie,Poland

Pore waters play an important role in phosphorus dynamics in aquatic ecosystems. Phosphorus concentrations in pore waters are much higher than above the bottom. This is confirmed by the results of this study concerning the hypereutrophic lake. Pore water was analyzed at 11 sampling stations in the upper layer of bottom sediments. This water was separated by centrifugation and phosphorus level was measured spectrophotometrically with ascorbic acid as a reducer. Total phosphorus concentration in pore waters ranged from 0.5 to 8.1 mgP l^− 1 (mean 3.2 mgP l^− 1). Mean phosphorus concentration in pore water samples of this lake was the highest in summer and the lowest in winter. High concentrations were observed in samples from the pelagial and low from the macrophyte zone.     

Toxicity assessment of sediments associated with the wreck of s/s Stuttgart in the Gulf of Gdańsk (Poland)

The paper presents the results of toxicity determinations carried out on sediment samples collected in the vicinity of the wreck of the German s/s Stuttgart (the southern part of the Gulf of Gdańsk, off the Polish coast) in relation to the determination of polycyclic aromatic hydrocarbons (PAHs). The toxicity of surface and core sediment samples was assessed using two biotest organisms-the bioluminescent bacteria Vibrio fischeri and the ostracod Heterocypris incongruens. PAH levels in these samples were determined by GC-MS. The surface sediments collected at W2 (ca. 34 m north of the shipwreck) and W4 (ca. 415 m north-east of the wreck) as well as the core sediments collected at WR3 (ca. 400 m north-east of the wreck) were the most heavily polluted with substances toxic to the biotest organisms. The chronic and acute toxicities in the case of most of the surface sediment samples studied are correlated. This may suggest that not only hydrophobic chemicals (like PAHs from fuel residues) but also more polar chemicals (resulting from the conversion of aromatic hydrocarbons) are responsible for the toxicity levels found. There is a clear dependence between the levels of chronic toxicity and PAH concentrations in the core sediment samples. In addition, a simultaneous decrease in the PAH content and chronic toxicity was noted in all the core sediment samples at depths below 80 cm.     

Storm water contamination and its effect on the quality of urban surface waters

We studied the effect of storm water drained by the sewerage system and discharged into a river and a small reservoir, on the example of five catchments located within the boundaries of the city of Poznań (Poland). These catchments differed both in terms of their surface area and land use (single- and multi-family housing, industrial areas). The aim of the analyses was to explain to what extent pollutants found in storm water runoff from the studied catchments affected the quality of surface waters and whether it threatened the aquatic organisms. Only some of the 14 studied variables and 22 chemical elements were important for the water quality of the river, i.e., pH, TSS, rain intensity, temperature, conductivity, dissolved oxygen, organic matter content, Al, Cu, Pb, Zn, Fe, Cd, Ni, Se, and Tl. The most serious threat to biota in the receiver came from the copper contamination of storm water runoff. Of all samples below the sewerage outflow, 74 % exceeded the mean acute value for Daphnia species. Some of them exceeded safe concentrations for other aquatic organisms. Only the outlet from the industrial area with the highest impervious surface had a substantial influence on the water quality of the river. A reservoir situated in the river course had an important influence on the elimination of storm water pollution, despite the very short residence time of its water.     

Concentrations of toxic heavy metals and trace elements in raw milk of Simmental and Holstein-Friesian cows from organic farm

Concentrations of toxic heavy metals (cadmium (Cd), lead (Pb)) and major nutritional and trace elements (Ca, Mg, P, Cu, Fe, Mn, Se, Zn) were analyzed in the milk of Simmental (n?=?20) and Holstein-Friesian (n?=?20) cows from an organic farm. Elements were determined using inductively coupled plasma emission atomic spectrometry. The conducted research showed that the milk of Simmental cows was characterized by the more advantageous mineral composition and lower concentration of noxious heavy metals compared to the milk of Holstein-Friesian cows. In the milk of Simmental cows, significantly lower concentrations of Pb and Cd (P?<?0.001) and Cu (P?<?0.05) and significantly higher concentrations of Fe and Mg (P?<?0.05) as well as nonsignificantly higher concentrations of Ca, Mn, and Se were found. In the milk of both breeds, very low Cu concentrations were recorded. The higher-than-recommended concentration of Pb in milk was also found. In the milk of both breeds, the significant positive correlations between concentrations of the following elements were observed: Pb–Cd, Pb–Se, Cd–Se, Cd–Mn, Zn–Cu, Zn–P, Ca–P, Ca–Mg, and Mg–P. The correlations between other elements within each of the analyzed breeds separately were also found.     

PM2.5 in the central part of Upper Silesia, Poland: concentrations, elemental composition, and mobility of components

The paper discusses ambient concentrations of PM_2.5 (ambient fine particles) and of 29 PM_2.5-related elements in Zabrze and Katowice, Poland, in 2007. The elemental composition of PM_2.5 was determined using energy dispersive X-ray fluorescence (EDXRF). The mobility (cumulative percentage of the water-soluble and exchangeable fractions of an element in its total concentration) of 18 PM_2.5-related elements in Zabrze and Katowice was computed by using sequential extraction and EDXRF combined into a simple method. The samples were extracted twice: in deionized water and in ammonium acetate. In general, the mobility and the concentrations of the majority of the elements were the same in both cities. S, Cl, K, Ca, Zn, Br, Ba, and Pb in both cities, Ti and Se in Katowice, and Sr in Zabrze had the mobility greater than 70%. Mobility of typical crustal elements, Al, Si, and Ti, because of high proportion of their exchangeable fractions in PM, was from 40 to 66%. Mobility of Fe and Cu was lower than 30%. Probable sources of PM_2.5 were determined by applying principal component analysis and multiple regression analysis and computing enrichment factors. Great part of PM_2.5 (78% in Katowice and 36% in Zabrze) originated from combustion of fuels in domestic furnaces (fossil fuels, biomass and wastes, etc.) and liquid fuels in car engines. Other identified sources were: power plants, soil, and roads in Zabrze and in Katowice an industrial source, probably a non-ferrous smelter or/and a steelwork, and power plants.     

Vegetation dynamics on the Łeba Bar/Poland: a comparison of the vegetation in 1932 and 2006 with special regard to endangered habitats

Coastal dune ecosystems are among the most dynamic habitats with high conservation value in Europe but are also under strong anthropogenic pressure regarding coastal protection and recreation. Hence, it is of high importance to know about long-term natural and anthropogenic changes and development of protected dune habitats for nature conservation, as well as for coastal management. This paper aims to identify the vegetation dynamics on the Łeba Bar/Poland over a period of 74 years by cartometric comparison using modern Geographical Information System (GIS). To quantify the rate of vegetation dynamics two aerial photographs dating from 1932 and 2006 were digitalized and analyzed with GIS to produce digital vegetation maps. Information about decrease, increase and stability of vegetation types of this area are discussed. The results show that there has been a clear reduction in the total area of bare sand and a considerable increase of woodlands and dense grass communities. Nevertheless, the remaining extensive drift sand areas and deflation hollows on the Łeba Bar offer one of the most important habitats for pioneer vegetation on bare sand of migrating dunes and dunes at the Southern Baltic coast. The present work proved the need to observe the future development of the vegetation communities and to implement management measures to maintain the dynamic of this unique dune landscape.     

The effect of bed properties on methane removal in an aerated biofilter--model studies

The capacity of laboratory-scale aerated biofilters to oxidize methane was investigated. Four types of organic and mineral-organic materials were flushed with a mixture of CH₄, CO₂ and air (1:1:8 by volume) during a six-month period. The filter bed materials were as follows: (1) municipal waste compost, (2) an organic horticultural substrate, (3) a composite of expanded perlite and compost amended with zeolite, and (4) the same mixture of perlite and compost amended with bentonite. Methanotrophic capacity during the six months of the experiment reached maximum values of between 889 and 1036 g m⁻² d⁻¹. Batch incubation tests were carried out in order to determine the influence of methane and oxygen concentrations, as well as the addition of sewage sludge, on methanotrophic activity. Michaelis constants K_M for CH₄ and O₂ were 4.6-14.9%, and 0.7-12.3%, respectively. Maximum methanotrophic activities V_max were between 1.3 and 11.6 cm³ g⁻¹ d⁻¹. The activity significantly increased when sewage sludge was added. The main conclusion is that the type of filter bed material (differing significantly in organic matter content, water-holding capacity, or gas diffusion coefficient) was not an important factor in determining methanotrophic capacity when oxygen was supplied to the biofilter.