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171.
Genetic variation in 15 Holothuria (Microthele) nobilis (Selenka, 1867) populations on the Great Barrier Reef was studied at seven polymorphic allozyme loci. Although populations
were separated by distances up to 1300 km, there were no apparent restrictions to gene flow (F
ST
values were not significantly different from 0) and the maximum Nei's unbiased genetic distance was 0.003. Populations were
in Hardy–Weinberg equilibrium at all loci. The estimated maximum sexual input [this is the ratio of the number of sexually
produced individuals (N*) to the sample size (N
i
)] and the minimum sexual input [this is the ratio of the number of genotypes (N
go
) over the sample size (N
i
)] were used as estimators for the amount of asexual reproduction. Both parameters suggested that H. nobilis reproduces solely by sexual means (N*:N
i
: = 1; N
go
:N
i
= 0.74 to 1). The allozyme data indicated high gene flow between populations, but the possibility that allozyme frequencies
may not be at equilibrium means that it was not possible to distinguish whether the patterns reflect present-day dispersal
or dispersal that occurred in the past.
Received: 27 March 2000 / Accepted: 29 June 2000 相似文献
172.
An experimental study on recolonization and succession of marine macrobenthos in defaunated sediment 总被引:3,自引:0,他引:3
Hypoxia/anoxia in coastal waters is a world wide problem which often results in mass mortality and defaunation of benthos.
In this study, field experiments were carried out to examine recolonization and succession of macrobenthic infauna in defaunated
sediments, and the time required for recovery from complete defaunation to a stable community. Trays (33 cm length × 25.5 cm
width × 11 cm depth) of defaunated sediment were exposed at the subtidal of a pristine site in subtropical Hong Kong. Temporal
changes of macrobenthic communities in defaunated sediment were analyzed by univariate and multivariate statistics, and compared
with those in undisturbed natural sediment at the same site. Initial colonization of macrobenthos occurred rapidly. A total
of 42 species was found, with an average of 258 animals per tray and 24 species per tray recorded in the first month. Abundance
showed a small peak (496 animals per tray) after 3 months, reached a sharp peak (1154 animals per tray) after 6 months, and
declined thereafter. Species number increased gradually, reached a maximum (68 species per tray) after 9 months, and then
decreased. Recolonization was predominantly contributed by larval settlement rather than adult migration. Temporal changes
in abundance, species number and diversity of the macrobenthic community in defaunated sediment resemble the spatial changes
along a decreasing pollution gradient previously defined by other authors. Results of this experiment suggest that newly available
sediment may allow more species to colonize (or coexist) than sediment pre-occupied by an established community. This is probably
due to less interspecific competition in the former habitat. No significant difference in abundance or species richness was
observed between defaunated and natural sediments after 15 months, suggesting that a stable community had been achieved, although
minor variations in species composition were still discernible between defaunated and natural sediments.
Received: 24 May 1999 / Accepted: 20 October 1999 相似文献
173.
R. Wilson G. Peters J. Regel D. Grémillet K. Pütz M. Kierspel H. Weimerskirch J. Cooper 《Marine Biology》1998,130(4):559-566
Stomach temperature loggers have proved useful for the study of feeding activity in free-living seabirds, but their usage
has been restricted because they are frequently dislodged and lost during the seabirds' regular regurgitation of indigestible
prey remains. In the present study we examine the incidence of spontaneous regurgitation (pellet production) in free-living
seabirds, consider the effect this has on the likely retrieval of stomach temperature loggers and present a structural modification
of the logger housings which leads to a much lower incidence of regurgitation. Systems were tested on albatrosses, cormorants,
gannets and penguins.
Received: 15 July 1997 / Accepted: 31 October 1997 相似文献
174.
Simultaneous hermaphroditism with outcrossing, a previously unconfirmed sexual system in decapod crustaceans, is documented
in the shrimp Lysmata wurdemanni (Gibbes), using time-lapse video observations on mating in pairs of “female-phase” (FP) individuals. Copulations between
FPs resulted in successful spawning and development of embryos. However, female-phase hermaphrodites maintained in isolation
were unable to self-fertilize spawned eggs. All smaller individuals possessed characters typical of caridean males, including
male gonopores, appendices masculinae, ejaculatory ducts, and cincinnuli on the first pleopods. However, the gonad of these
male-phase (MP) individuals was an ovotestis with an undeveloped ovarian portion. FPs, which spawn eggs and incubate embryos,
also had male gonopores and an ovotestis terminating in ejaculatory ducts containing sperm. In FPs, male pleopod characters
were absent or reduced, and a female incubatory character, expanded pleopod flanges, showed a greater relative size. Smaller
size classes of the population were composed only of MPs while larger size classes consisted of both MPs and FPs. “Transitionals,”
individuals with MP characters but an ovotestis containing vitellogenic oocytes, were rare and overlapped completely in size
with FP and larger MP individuals. It is suggested that not all MPs transform into FP hermaphrodites. Social interactions
may mediate MP change into FP hermaphrodites in L. wurdemanni. The social system of L. wurdemanni and other Lysmata spp. may determine the exact form of the sexual system in these species.
Received: 26 October 1997 / Accepted: 21 April 1998 相似文献
175.
The Norway lobster Nephrops norvegicus (L.) inhabits burrows in muddy clay sediments (e.g. on the Swedish west coast), where an autumnal oxygen deficiency in the
bottom water can occur. Our experiments investigated whether the irrigation of the burrows would reflect a behavioural adaptation
to hypoxia, and whether any gender differences of such behaviour exist. Irrigation is performed by the pleopods which may
compensate for a decreasing oxygen tension. Pleopod activity (total number of strokes per sampling time), associated with
oxygen concentration and gender, was studied in N. norvegicus kept in artificial burrows resembling their natural habitat. Male and female lobsters were separately exposed to either normoxia
(70% oxygen saturation) or hypoxia (30% oxygen saturation). A sexual difference in behaviour was found, where females irrigated
the burrow less than males during normoxia. Females showed a significant increase of pleopod activity in hypoxia compared
with normoxic conditions, which was not displayed by the males probably due to the degree of individual variation found. However,
when only males were studied during progressive hypoxia (from 60 to 5% oxygen saturation), following any changes of irrigational
behaviour, a significant increase of accumulated pleopod activity occurred. A major increase of pleopod activity appeared
between 60 and 50% oxygen saturation, below which the activity remained high until a critical point (<10% saturation, 11 °C,
33 psu) where irrigation dropped to a level close to that of normoxic values. Activity sessions during hypoxia were longer
and had a higher stroke rate than during normoxia.
Received: 22 October 1997 / Accepted: 26 February 1998 相似文献
176.
The sibling species, Pseudocalanus moultoni (Frost, 1989) and P. newmani (Frost, 1989), occur sympatrically on Georges Bank. Taxonomic discrimination of the species relies on subtle morphological
characteristics, making routine identification of the species very difficult. DNA sequence variation of two mitochondrial
genes, 16S rRNA and cytochrome oxidase I (COI), reliably discriminated P. moultoni and P.␣newmani. Levels of DNA sequence variation for both genes were consistent with those between species of calanoid copepods. A molecular
systematic protocol (based on allele-specific PCR amplification) was designed from the COI sequences and used to discriminate
females of the two species. The distributions and relative abundances of the two species were mapped for April 1996 based
on samples of 15 to 30 females from 12 collections across Georges Bank. The results of this study indicated that P. moultoni females predominated along the northern flank of Georges Bank, while P. newmani females were common on the southern flank, deeper than the 60-m isobath.
Received: 16 May 1997 / Accepted: 26 April 1998 相似文献
177.
Recovery of benthic communities at Macquarie Island (sub-Antarctic) following a small oil spill 总被引:5,自引:0,他引:5
Intertidal communities at Macquarie Island (sub-Antarctic) were re-evaluated seven years after a small oil spill resulting
from the grounding of the “Nella Dan”, and six years after the first assessment of biological impact. Sampling was conducted
to evaluate community structure in three zones of the exposed rocky shore (upper red, kelp, and lower red) and in samples
of Durvillaea antarctica holdfasts. There were no significant differences between the community structure in oiled and control locations in any of
the three shore zones, but holdfast macrofaunal communities at oiled sites still showed evidence of impact. Holdfast community-structure
in samples from heavily oiled sites showed moderate levels of recovery, with increased abundances of species which were considered
sensitive to the oiling in the first post-impact studies and decreased abundances of opportunistic polychaete and oligochaete
worms. In contrast, samples collected from the moderately oiled location at Secluded Bay showed little evidence of recovery.
Holdfasts at this site were filled with sediment containing traces of diesel oil, and the macrofaunal community was dominated
by opportunistic worms. This study clearly indicates that even small incidents of anthropogenic perturbation can have long-lasting
consequences for marine communities at Macquarie Island.
Received: 23 July 1997 / Accepted: 5 February 1998 相似文献
178.
Previously published mtDNA cytochrome b sequences for Xyrichtys novacula (Linnaeus) and two morphologically distinct types of Xyrichtys larvae (Pisces: Labridae) included human cytochrome b sequence, presumably due to sample contamination and/or poor preservation of sample DNA. Those sequences had been used to
identify the two types of Xyrichtys larvae as X. novacula, but owing to the contamination, the identifications are invalid. Fresh specimens were collected: X.␣martinicensis (Cuvier and Valenciennes), X. novacula, X. splendens (Castelnau), and Xyrichtys sp. larvae of each of the two morphotypes (dorsal-forward eye and ventral-forward eye). The cytochrome b fragment was amplified from each specimen using the polymerase chain reaction. Comparison of the sequences with human cytochrome b sequence confirmed that the new sequences were not contaminated. The interspecific differences in Xyrichtys sequences were less than previously reported, but still greater than observed in many fish genera. Distance and parsimony
analyses indicated that X. novacula and X. martinicensis were more closely related to each other than to X. splendens. This conclusion differs from our previous conclusion that X. martinicensis and X. splendens were more closely related to each other than to X. novacula. Distance and parsimony analyses also demonstrated that both larval morphotypes were X. novacula and, thus, our previous conclusions regarding larval ecology and oceanographic transport remain unchanged.
Received: 12 March 1997 / Accepted: 24 October 1997 相似文献
179.
Through two-choice gustatory experiments, a␣preference hierarchy was established␣for␣the␣herbivorous gastropod Norrisianorrisi Sowerby, with laminarialean kelps preferred over all other seaweeds. Among the kelps, laminae of Macrocystispyrifera were slightly preferred over Egregiamenziesii, and both were strongly preferred over sporophylls of Eiseniaarborea. E.arborea, the least preferred kelp, was consistently chosen over other algae common in the snail's habitat (Halidrysdioica, Dictyotaflabellata, and Pterocladiacapillacea) and over seaweeds believed to be edible and palatable based on their morphology, structure, and secondary chemistry (Endarachnebinghamiae, Mazzaellaflaccida, and Ulvalobata). The morphologies and structural toughness of tested seaweeds varied significantly as did their nutritional (% carbon, %
nitrogen, C:N ratio, and % ash) contents and phlorotannin concentrations; however, snails preferred to feed on kelps regardless
of nutritional content, toughness or phlorotannin concentration; and among kelps preferred to feed on the least tough species
(based on penetrometer measurements), which also were those containing the lowest phlorotannin concentrations. Preference
for kelp was not upheld in experiments using agarose thalli to which freeze-dried powder, of either the kelp Eiseniaarborea or non-kelp Endarachnebinghamiae was added, suggesting the destruction of attractant chemicals during the making of the artificial foods. Our data suggest
that the preference of N. norrisi for kelps over other potentially edible and palatable seaweeds may not be related to nutritional content, but instead may
have evolved in response to factors such as availability, habitat provision, or refuge from predation.
Received: 27 September 1996 / Accepted: 7 October 1997 相似文献
180.
The pattern of settlement over time of three broadcast spawning coral species (Cyphastrea serailia, Acanthastrea lordhowensis, and Goniastrea australensis) from the Solitary Islands (30°00′S; 153°20′E) was studied in 1995 and 1996 in order to determine the maximum length of time
these larvae could remain in the water column and still retain the ability to settle and metamorphose. Larvae were maintained
in aquaria and the number which had settled on biologically-conditioned tile pairs was monitored every 5 to 10 d. While the
majority of larvae settled quickly after becoming competent, some larvae survived and settled for extended periods after spawning.
Competency periods ranged from 26 d for C. serailia to 56 d for G. australensis and 78 d for A. lordhowensis. These data greatly extend the known competency periods for larvae of broadcast-spawning corals and indicate the potential
for transport of broadcast-spawned coral larvae over large distances. Medium to long-distance larval dispersal of the species
studied provides a mechanism for their widespread distribution in subtropical regions, on reefs which are often widely spaced
and relatively isolated.
Received: 27 May 1997 / Accepted: 27 November 1997 相似文献