Mixing-controlled biodegradation in a toluene plume--results from two-dimensional laboratory experiments

Various abiotic and biotic processes such as sorption, dilution, and degradation are known to affect the fate of organic contaminants, such as petroleum hydrocarbons in saturated porous media. Reactive transport modeling of such plumes indicates that the biodegradation of organic pollutants is, in many cases, controlled by mixing and therefore occurs locally at the plume's fringes, where electron donors and electron-acceptors mix. Herein, we aim to test whether this hypothesis can be verified by experimental results obtained from aerobic and anaerobic degradation experiments in two-dimensional sediment microcosms. Toluene was selected as a model compound for oxidizable contaminants. The two-dimensional microcosm was filled with quartz sand and operated under controlled flow conditions simulating a contaminant plume in otherwise uncontaminated groundwater. Aerobic degradation of toluene by Pseudomonas putida mt-2 reduced a continuous 8.7 mg L(-1) toluene concentration by 35% over a transport distance of 78 cm in 15.5 h. In comparison, under similar conditions Aromatoleum aromaticum strain EbN1 degraded 98% of the toluene infiltrated using nitrate (68.5+/-6.2 mg L(-1)) as electron acceptor. A major part of the biodegradation activity was located at the plume fringes and the slope of the electron-acceptor gradient was steeper during periods of active biodegradation. The distribution of toluene and the significant overlap of nitrate at the plume's fringe indicate that biokinetic and/or microscale transport processes may constitute additional limiting factors. Experimental data is corroborated with results from a reactive transport model using double Monod kinetics. The outcome of the study shows that in order to simulate degradation in contaminant plumes, detailed data sets are required to test the applicability of models. These will have to deal with the incorporation of existing parameters coding for substrate conversion kinetics and microbial growth.     

Initial Changes in Refilled Lysimeters Built with Metal Polluted Topsoil and Acidic or Calcareous Subsoils as Indicated by Changes in Drainage Water Composition

Soil translocation for recultivation of soil removed from construction sites and for the preparation of refilled lysimeters inevitably involves disturbance of soil structure, and, if intermediate storage is included, also drying and rewetting of the soil. We report on an experiment with model forest ecosystems, where uncontaminated forest subsoils were covered with non-contaminated or freshly heavy metal (mainly Zn and Cu) contaminated topsoil in large lysimeters. Monitoring of the chemical composition of the drainage water revealed two distinct soil conditioning phases. During an initial phase of about a year strongly elevated nitrate and sulfate concentrations occurred that were attributed to a mineralisation flush caused by the increased accessability of mineralisable nitrogen and sulfur in destroyed aggregates. These effects were significantly larger in lysimeters with calcareous subsoil than in those with acidic subsoil. The second phase was characterised by a gradual decrease in dissolved organic carbon and sulfate concentrations, in particular in the acidic subsoil. This decrease may be attributed to the depletion of pools made accessible during aggregate destruction or the formation of new aggregates. These chemical changes had only little effects on the concentrations of copper and zinc in the drainage water. Based on our results, it can be concluded that large refilled lysimeters can be used for many purposes without risk of compromised results, if a conditioning phase of about 1 year with sufficiently moist soil conditions is respected. Nevertheless, gradual changes in soil chemical characteristics still occur after this initial phase. Implications for the recultivation of sites using relocated soils are discussed.     

Chemisch-analytische Untersuchung von Arsenkampfstoffen und ihren Metaboliten

Arsenic containing chemical warfare agents (cwa) are persistent in the environment and continue to contaminte soil and ground water. In this paper the chemical rections of the cwa diphenylarsine chloride (CLARK I), phenylarsine dichloride (PFIFFIKUS), ethylarsine dichloride (DICK) and of chlorovinyl arsenic compounds (LEWISITE) are shown. The posibilities of the detection and determination of this cwa and their metabolites are presented.     

Passivsammler zur Wasseruntersuchung

The topography and motion of contaminant plumes in groundwater may be determined by surveying the three-dimensional distribution of contaminant freights [1] as well as through a period-integral monitoring by contaminant dosimeters [1]. Conventional methods of taking water samples for chemical analysis cannot fulfill any of these requirements. Sorption active passive collectors suitable for corresponding surveys in gaseous phases are unsuitable for examination in aquifers. Passive collectors for the examination of undisturbed aquifers represent the central part of a probing device system developed by the authors for monitoring chemical and hydraulic parameters within all kinds of aquifers. These passive collectors permit both a determination of the relative contaminant freights at deliberate vertical spacing of vertically ordered measuring points, and period-integrating detection of vertical profiles of relative contaminant freights. The easy-to-handle passive collectors are suitable for the collection of organic and inorganic contaminants and may be evaluated by standardized chemical analysis methods. In this way, passive collectors fulfill the essential prerequisites for purpose-oriented monitoring of contaminant plumes. Moreover, passive collectors for ground water monitoring are well suited for the in situ-examination of any other aquifer, both still and fluent, regardless of its depth or extent. Examples of application may be sewage ducts, sewage plants, rivers, lakes, oceans, water samples and especially the identification of contaminants and trace substances.     

False-negative prenatal exclusion of Wiskott-Aldrich syndrome by measurement of fetal platelet count and size

The study of the fetal platelet count and size can, according to the literature, be used for the prenatal diagnosis of the Wiskott-Aldrich syndrome (WAS). So far, no affected fetuses have been identified by this method. All pregnancies in which this method had been applied to resulted, as correctly predicted, in the birth of normal children. Here we report on a familial case of WAS where the haematological parameters failed to reveal the affected second child. Hence we assume that the platelet count and size of platelets remain normal in fetuses with WAS to the gestational age of 22 weeks and cannot be used for prenatal diagnosis.     

Prenatal diagnosis of aortic atresia by colour doppler flow mapping

A case of aortic atresia with insufficiency of mitral valve diagnosed prenatally at 33 weeks of gestation is presented. An accurate diagnosis of this fetal cardiovascular malformation was possible by application of Doppler colour flow mapping, which demonstrated (a) the absence of forward flow in the hypoplastic ascending aorta, (b) reverse flow of blood from the ductus arteriosus into the severely hypoplastic ascending aorta in the late systole, (c) pansystolic mitral valve regurgitation, and (d) absent flow across the foramen ovale as a result of premature closure of the foramen ovale.     

Seeufer 2003 — Ökologie, Bewertung, Nachhaltige Entwicklung

This report of an international conference about the problems around lakeshores, their evaluation and the possibilities to improve the situation for lake shores, continues the series ‘Lake shores, a forgotten ecotone’ as article 4. Five main topics were discussed: (1) applied ecology of lakeshores, (2) human impact, (3) ecological assessment, (4) protection and restoration, (5) sustainable development and use of lake shores.     

Nematodentest

For the assessment of contaminated and remediated soils and substrates, and the estimation of chronic toxicity of soil contaminations, an ecotoxicological test was developed using the terrestrial nematodePanagrellus redivivus. The population dynamics expressed by the reproduction factor (VF) allows an estimation of soil quality within seven days. Due to the rapid generation time ofPanagrellus (< 5 d), the short test duration is considered to be sufficient. The suitability of this test duration is considered to be sufficient. The suitability of this test was evaluated by investigating soils which had been contaminated using applications of specific pollutants (lindane and PCB 52).