Biomarkers and bioindicators of the health condition of Ameca splendens and Goodea atripinnis (Pisces: Goodeaidae) in the Ameca River, Mexico

Pollution of water bodies causes stress on organisms inhabiting them. Determination of biomarkers and bioindicators on fish populations reflects whether they are subject to stress. We assessed two populations of Ameca splendens and Goodea atripinnis in a reference site (spring "El Rincon") and De La Vega reservoir, which receives wastewater of a sugar industry, on Ameca River course. We analyzed level of lipid peroxidation and enzymatic activities of gamma-glutamyl-transpeptidase, acetylcholinesterase and ethoxyresorufin-O-deethylase as biomarkers; we also studied age classes and various body indexes. Environmental factors were recorded and a water quality index was assessed. Water quality was better in the spring than in the reservoir. Organisms inhabiting the reservoir presented higher oxidative stress by the lipid peroxidation levels, and neurotoxic impacts by the acetylcholinesterase and some detoxification mechanisms were evident by the gamma-glutamyl-transpeptidase and ethoxyresorufin-O-deethylase activities. Integrated Biomarker Response demonstrate that De La Vega reservoir is a more stressing place to organisms living there, particularly for A. splendens. In both species, males were more affected than females. Condition and reproductive parameters in reservoir showed evidences of physiological changes due to xenobiotics exposure and suggest a tactic of the organisms to survive in this site. Both biomarkers and body indexes revealed that A. splendens is a more sensitive species than G. atripinnis to environmental stress.     

Life cycle assessment tool of electricity generation in Portugal

This paper investigates multi-stakeholder arrangements initiated by businesses and NGOs from the North that aim to enhance a more sustainable agricultural production at specific localities in Southern countries. We aim to better understand the search for concerted action in multi-actor arrangements. Therefore, this paper presents a diagnostic framework with three strategic challenges the partnership projects are facing: linking global economic objectives to local needs, values and interests; bridging public and private interests and responsibilities; and seeking trade-offs between social, environmental and economic values. Starting from the partnerships’ Theory of Change, this diagnostic framework is applied to comparative case studies of partnership projects in the cocoa sector in Indonesia, which are part of a Northern-based public–private partnership to improve farmers’ prospective. It is concluded that the economic reality faced by the farmers differs from that of the Northern actors; collaboration with governments is difficult because of different organizational cultures; and the partnership projects underestimate the strength of vested social relations the smallholders are part of. Overall, the initiators of the partnerships seem to work with a too restricted economic interpretation of the local reality.     

Towards a climate change adaptation strategy for coffee communities and ecosystems in the Sierra Madre de Chiapas, Mexico

The mountain chain of the Sierra Madre de Chiapas in southern Mexico is globally significant for its biodiversity and is one of the most important coffee production areas of Mexico. It provides water for several municipalities and its biosphere reserves are important tourist attractions. Much of the forest cover outside the core protected areas is in fact coffee grown under traditional forest shade. Unless this (agro)forest cover can be sustained, the biodiversity of the Sierra Madre and the environmental services it provides are at risk. We analyzed the threats to livelihoods and environment from climate change through crop suitability modeling based on downscaled climate scenarios for the period 2040 to 2069 (referred to as 2050s) and developed adaptation options through an expert workshop. Significant areas of forest and occasionally coffee are destroyed every year by wildfires, and this problem is bound to increase in a hotter and drier future climate. Widespread landslides and inundations, including on coffee farms, have recently been caused by hurricanes whose intensity is predicted to increase. A hotter climate with more irregular rainfall will be less favorable to the production of quality coffee and lower profitability may compel farmers to abandon shade coffee and expand other land uses of less biodiversity value, probably at the expense of forest. A comprehensive strategy to sustain the biodiversity, ecosystem services and livelihoods of the Sierra Madre in the face of climate change should include the promotion of biodiversity friendly coffee growing and processing practices including complex shade which can offer some hurricane protection and product diversification; payments for forest conservation and restoration from existing government programs complemented by private initiatives; diversification of income sources to mitigate risks associated with unstable environmental conditions and coffee markets; integrated fire management; development of markets that reward sustainable land use practices and forest conservation; crop insurance programs that are accessible to smallholders; and the strengthening of local capacity for adaptive resource management.     

Differential aggregation of polystyrene and titanium dioxide nanoparticles under various salinity conditions and against multiple proteins types

The interaction of nanoplastics (NPls) and engineered nanoparticles (ENPs) with organic matter and environmental pollutants is particularly important. Therefore, their behavior should be investigated under the different salinity conditions, mimicking rivers and coastal environments, to understand this phenomenon in those areas. In this work, we analyzed the elementary characteristics of polystyrene-PS (unmodified surface and modified with amino or carboxyl groups) and titanium dioxide-TiO2 nanoparticles. The effect of salinity on their colloidal properties was studied too. Also, the interaction with different types of proteins (bovine serum albumin-BSA and tilapia proteins), as well as the formation of the BSA corona and its effect on the colloidal stability of nanoparticles, were evaluated. The morphology and dispersion of sizes were more uniform in unmodified-surface PS-NPs (70.5?±?13.7 nm) than in TiO2-NPs (131.2?±?125.6 nm). Likewise, Rama spectroscopy allowed recognizing peaks associated with the PS phenyl group aromatic ring in unmodified-surface PS-NPs (621, 1002, 1582, and 1602 cm^?1). For TiO2-NPs, the data suggest belonging to the tetragonal form, also known as rutile (445, 610 cm^?1). The elevation of salinity dose-dependently decreased NP colloid stability, with more significant variation in the PS-NPs compared to TiO2-NPs. The organic matter is also involved in this phenomenon, differentially as a function of time compared to its absence (unmodified-surface PS-NPs 30 psu/TOC 5 mgL^?1/24 h: 2876.6?±?378.03 nm; unmodified-surface PS-NPs 30 psu/24 h: 2133?±?49.57 nm). In general, the TiO2-NPs demonstrated greater affinity with all proteins tested (0.066 g/L). It was observed that morphology, size, and surface chemical modification intervene in a relevant way in the interaction of the nanoparticles with bovine serum albumin (unmodified-surface PS-NPs 298 K: 6.08E⁺⁰²; 310 K: 6.63E⁺⁰²; TiO2-NPs 298 K: 8.76E⁺⁰²; 310 K: 1.05E⁺⁰³ L mol^?1) and tilapia tissues proteins (from blood, gills, liver, and brain). Their morphology and size also determined the protein corona formation and the NPs’ agglomeration. These findings can provide references during knowledge transfer between NPls and ENPs.

     

Isolation of mesotrione-degrading bacteria from aquatic environments in Brazil

Mesotrione is a benzoylcyclohexane-1,3-dione herbicide that inhibits 4-hydroxyphenyl pyruvate dioxygenase in target plants. Although it has been used since 2000, only a limited number of degrading microorganisms have been reported. Mesotrione-degrading bacteria were selected among strains isolated from Brazilian aquatic environments, located near corn fields treated with this herbicide. Pantoea ananatis was found to rapidly and completely degrade mesotrione. Mesotrione did not serve as a sole C, N, or S source for growth of P. ananatis, and mesotrione catabolism required glucose supplementation to minimal media. LC-MS/MS analyses indicated that mesotrione degradation produced intermediates other than 2-amino-4-methylsulfonyl benzoic acid or 4-methylsulfonyl-2-nitrobenzoic acid, two metabolites previously identified in a mesotrione-degrading Bacillus strain. Since P. ananatis rapidly degraded mesotrione, this strain might be useful for bioremediation purposes.     

Assessment of organochlorine pesticide residues in Atlantic Rain Forest fragments, Rio de Janeiro, Brazil

A superficial water quality survey in a watershed of the Paraíba do Sul River, the main water supply for the most populated cities of southeastern Brazil, was held in order to assess the impact of the expansion of agricultural activity in the near border of the Atlantic Rain Forest. The aim of this study was to investigate the presence of priority organochlorine pollutants in soils and superficial waters of Atlantic rainforest fragments in Teresópolis, Rio de Janeiro State. Soil sample preparations were compared by using ultrasound, microwave assisted extraction and Soxhlet extraction. Recoveries of matrix spiked samples ranged from 70 to 130%. Analysis of a certified soil material showed recoveries ranging from 71 to 234%. Although low concentrations of organochlorine residues were found in water and soil samples, this area is of environmental importance and concern, thus demanding a monitoring program of its compartments.     

Cytogenetic biomonitoring of oral mucosa cells of crack cocaine users

The aim of the present study was to comparatively evaluate genomic damage (micronucleus) and cellular death (pyknosis, karyolysis, and karyorrhexis) in exfoliated oral mucosa cells from crack cocaine users by micronucleus test. A total of 30 crack cocaine users and 30 health controls (non-exposed individuals) were included in this setting. Individuals had epithelial cells from cheek mechanically exfoliated, placed in fixative, and dropped in clean slides, which were checked for the above nuclear phenotypes. The results pointed out significant statistical differences (p?<?0.05) of micronucleated oral mucosa cells from crack cocaine users. Exposure to crack cocaine caused an increase of other nuclear alterations closely related to cytotoxicity such as karyolysis in oral cells as well. In summary, these data indicate that crack cocaine is able to induce chromosomal breakage and cellular death in oral mucosa cells of users.     

A One Year Study on the Concentrations of Norovirus and Enteric Adenoviruses in Wastewater and A Surface Drinking Water Source in Norway

Enteric viruses transmitted via the faecal-oral route occur in high concentrations in wastewater and may contaminate drinking water sources and cause disease. In order to quantify enteric adenovirus and norovirus genotypes I and II (GI and GII) impacting a drinking source in Norway, samples of surface water (52), wastewater inlet (64) and outlet (59) were collected between January 2011 and April 2012. Samples were concentrated in two steps, using an electropositive disc filter and polyethylene glycol precipitation, followed by nucleic acid extraction and analysis by quantitative polymerase chain reaction. Virus was detected in 47/52 (90.4 %) of surface water, 59/64 (92 %) of wastewater inlet and 55/59 (93 %) of wastewater outlet samples. Norovirus GI occurred in the highest concentrations in surface water (2.51e + 04) and adenovirus in wastewater (2.15e + 07). While adenovirus was the most frequently detected in all matrices, norovirus GI was more frequently detected in surface water and norovirus GII in wastewater. This study is the first in Norway to monitor both sewage and a drinking water source in parallel, and confirms the year-round presence of norovirus and adenovirus in a Norwegian drinking water source.     

Ecological restoration as a strategy for mitigating and adapting to climate change: lessons and challenges from Brazil

Climate change is a global phenomenon that affects biophysical systems and human well-being. The Paris Agreement of the United Nations Framework Convention on Climate Change entered into force in 2016 with the objective of strengthening the global response to climate change by keeping global temperature rise this century well below 2 °C above pre-industrial levels and to pursue efforts to limit the temperature increase even further to 1.5 °C. The agreement requires all Parties to submit their “nationally determined contributions” (NDCs) and to strengthen these efforts in the years ahead. Reducing carbon emissions from deforestation and forest degradation is an important strategy for mitigating climate change, particularly in developing countries with large forests. Extensive tropical forest loss and degradation have increased awareness at the international level of the need to undertake large-scale ecological restoration, highlighting the need to identify cases in which restoration strategies can contribute to mitigation and adaptation. Here we consider Brazil as a case study to evaluate the benefits and challenges of implementing large-scale restoration programs in developing countries. The Brazilian NDC included the target of restoring and reforesting 12 million hectares of forests for multiple uses by 2030. Restoration of native vegetation is one of the foundations of sustainable rural development in Brazil and should consider multiple purposes, from biodiversity and ecosystem services conservation to social and economic development. However, ecological restoration still presents substantial challenges for tropical and mega-diverse countries, including the need to develop plans that are technically and financially feasible, as well as public policies and monitoring instruments that can assess effectiveness. The planning, execution, and monitoring of restoration efforts strongly depend on the context and the diagnosis of the area with respect to reference ecosystems (e.g., forests, savannas, grasslands, wetlands). In addition, poor integration of climate change policies at the national and subnational levels and with other sectorial policies constrains the large-scale implementation of restoration programs. The case of Brazil shows that slowing deforestation is possible; however, this analysis highlights the need for increased national commitment and international support for actions that require large-scale transformations of the forest sector regarding ecosystem restoration efforts. Scaling up the ambitions and actions of the Paris Agreement implies the need for a global framework that recognizes landscape restoration as a cost-effective nature-based solution and that supports countries in addressing their remaining needs, challenges, and barriers.