D-Pulegone: A feeding deterrent to deer mice (Peromyscus maniculatus) and prairie voles (Microtus ochrogaster)

Summary D-Pulegone, a monoterpene present in pennyroyålMentha sp. is repellent to a number of vertebrates, decreasing consumption of feed and granular particles. In the present study, several tests were used to determine the reliability of this substance as a feeding deterrent. D-Pulegone (10mM—1M) applied as a coating to apple pieces significantly (P < 0.001) decreased ingestion by deer mice,Peromyscus maniculatus, and prairie voles,Microtus ochrogaster. The deterrent effect of d-pulegone was not diminished following multiple exposures suggesting that this compound may be a promising candidate for use a a general vertebrate repellent. The inhibitory effect of 1M d-pulegone on apple ingestion in voles was abolished when contact was prevented by encasing the stimulus solution in plastic mesh capsules. We conclude that while d-pulegone has repellent properties following direct contact, volatile exposure alone may not be sufficient to elicit avoidance behavior.     

Problems of the gonad index and what can be done: analysis of the purple sea urchin <Emphasis Type="Italic">Strongylocentrotus purpuratus</Emphasis>

The gonad index, GI, is widely used as a measure of changes in reproductive state. There are, however, problems with its use because it is based on the implicit assumption of an isometric relationship between gonad size and some measure of total size. If, for example, gonad weight and total weight are used, the exponent for an allometric relationship usually is ignored and hence assumed to be 1.0. It is further assumed that this exponent is fixed for all states of the reproductive cycle and that gonads begin to develop at size = 0. Data for the purple sea urchin Strongylocentrotus purpuratus at Gregory Point, Oregon, USA, gathered over a period of 31 months showed that these assumptions cannot be supported. The relationship is better modeled with a function that (1) takes into account size of initial gonad production and (2) allows allometric exponents that vary with site or season. Thus, a better approach is to use a wide range of sizes to estimate size when gonads begin to develop and then, with this correction, ANCOVA to test for differences of gonad size among samples. Gonad changes at Gregory Point were estimated using fixed sizes of 5 cm diameter and 60 g total weight. Publishing means for X and Y, the standard error of the estimate, R ², and slope for each regression are shown to be sufficient to compare our results with results across studies.     

Runoff transport of pyrethroids from a residential lawn in central California

An irrigation runoff study on a residential lawn was conducted in California, northeast of Sacramento, during the summer and fall of 2008 to investigate the contribution of turf uses of pyrethroids to residues in Californian urban creek sediments. This study examined how over irrigation (i.e., irrigation that produces runoff) in the summer season may transport recently applied pyrethroids. The study included liquid and granular applications of both bifenthrin [(2-methyl-3-phenyl-phenyl) methyl 3-(2-chloro-3,3,3-trifluoro-prop-1-enyl)-2,2-dimethyl-cyclopropane-1-carboxylate] and beta-cyfluthrin [Cyano(4-fluoro-3-phenoxyphenyl)methyl 3-(2,2-dichloroethenyl)-2,2-dimethyl-cyclopropanecarboxylate]. Generally, runoff did not occur at irrigation rates of 2.03 cm/h (0.8 in/h) but did occur when the irrigation rates were increased to about 3.81 cm/h (1.5 in/h), generating chemical losses in the first runoff event of up to 0.58 and 0.08% of applied for beta-cyfluthrin and bifenthrin, respectively. Chemical runoff losses dropped significantly between over-irrigation events with the third over-irrigation event chemical runoff losses representing 0.026 and 0.015% of applied for beta-cyfluthrin and bifenthrin, respectively. Runoff losses were generally less for liquid formulations than granular formulations but within a factor of three. Additionally, the study included a simulated winter rainstorm 8 wk after application. The low runoff losses from turf seen in this study suggest that other sources could be contributing to observed residues in urban streams. Other sources could include pyrethroids ending up on impervious surfaces, such as concrete driveways from off-target applications to turf, spills, and other poor handling practices, or pyrechroids applied directly to impervious surfaces for insect control.     

Modelling the effects of ‘coastal’ acidification on copper speciation

We present here a copper speciation model that accounts for the long-term (‘coastal-acidification’) and short-term (daily and seasonal variation) variability in water pH and water temperature. The developed model is applied to a sub-tropical estuary (Moreton Bay, Australia) at a one hundred year time scale so that outputs are consistent with climate change projections. The model predicts that the mean cupric ion concentration (Cu²⁺) in the estuary will increase by 115% over the next 100 years as a result of the projected decrease in pH and increase in water temperature. Through calibration, the estimated concentration of copper-complexing dissolved organic matter (DOM) in the estuary is found to be 22.5 nM. An increase in the concentration of Cu²⁺, which is the most toxic and bioavailable form of copper, has implications for ecosystem health and may have a negative effect on the detoxifying capacity of DOM. Models that provide a framework for coupling biological, chemical and physical processes are important for providing a holistic perspective of coastal systems, especially for better understanding a system within the context of climatic and non-climatic drivers.     

Death awareness and organizational behavior

This Incubator introduces scholars to death awareness research. We explicate how mortality salience can be studied in conjunction with workplace behavior. Using the Terror Management Theory (TMT) framework, we explore morality salience in combination with several workplace phenomena, including aggression, discrimination, and punishment; and, several ideas for future research are suggested. Copyright © 2010 John Wiley & Sons, Ltd.     

The European eel quality database: towards a pan-European monitoring of eel quality

The stocks of the European eel Anguilla anguilla are in decline and there is an increasing awareness that poor health status due to contaminants and/or diseases might be a key element in this decline and might be a hindrance to recovery. Many countries have started compiling data on the health status of eels in their water bodies. Objectives for these monitoring actions are diverse and there is a large amount of information collected by EU member countries. However, this information is widely scattered over Europe in agencies, institutes or universities. As there is a growing need to collect and report on data on the health status of the eel on international level, the Joint EIFAC/ICES Working Group on Eels initiated in September 2007 the set up of an European Eel Quality Database to collect recent data of contaminants and diseases over the distribution area of the eel. This paper describes the aim, the set up and future development of the database in order to give it greater publicity and to call on scientists or managers to submit data on eel health status. The database represents now the first comprehensive pan-European compilation of eel health data, including data from over 10,000 eels from approximately 1,200 sites over 14 countries. Preliminary work has indicated a number of shortcomings and future developments will be needed. Guaranteeing further development of the database, harmonisation of methods, quality assurance, and setting up harmonised eel monitoring strategies over Europe will be a great challenge and will need pan-European cooperative work.     

Sources and fate of Salmonella and fecal indicator bacteria in an urban creek

This research aimed to understand the sources and fate of Salmonella and fecal bacteria in urban surface waters. An urban creek (San Pedro Creek, California, USA) that had unusually high levels of Salmonella and fecal bacteria relative to other nearby waterbodies was chosen as a model field site. State of the art microbiological methods were used in concert with modeling to investigate Salmonella and fecal bacteria sources, and determine field-relevant dark inactivation and photoinactivation rates. Three along-creek surveys that spanned reaches adjacent to both urban and forested land covers were conducted to measure Salmonella, enterococci, Escherichia coli, and horse- and human-specific Bacteroidales. Salmonella were detected adjacent to and downstream of urban land cover, but not adjacent to forested land cover. No human or horse-specific Bacteroidales fecal markers were detected implicating other urban animal sources of bacteria. Two locations along the creek where Salmonella was consistently detected were sampled hourly for 25 hours and a mass-balance model was applied to determine field-relevant light and dark inactivation rates for Salmonella, enterococci, and E. coli. Sunlight inactivation did not appear to be important in modulating concentrations of Salmonella, but was important in modulating both enterococci and E. coli concentrations. Dark inactivation was important for all three organisms. This is the first study to quantitatively examine the fate of Salmonella within an urban surface water. Although the work is carried out at a single site, the methodologies are extendable to source tracking in other waterbodies. Additionally, the rate constants determined through the modeling will be useful for modeling these organisms in other surface waters, and represent useful benchmarks for comparison to laboratory-derived inactivation rates.     

Laboratory and field evaluation of instrumentation for the semicontinuous determination of particulate nitrate (and other water-soluble particulate components)

Studies conducted at the U.S. Environmental Protection Agency facility in Research Triangle Park, NC, and at a field study in Southern California have demonstrated the capability for the semicontinuous determination of particulate nitrate (and other water-soluble ionic species). Two instruments, an R&P Series 8400N Ambient Particulate Nitrate Monitor (8400N) and an ion chromatography (IC)-based prototype monitor developed at Texas Tech University, were evaluated both in the laboratory using aqueous standards and simulated ambient aerosols and in the field during a 3-week July 1-21, 2003) joint ambient comparison study with Brigham Young University and the South Coast Air Quality Management District (SCAQMD). The field study was conducted at the SCAQMD Rubidoux field site near Riverside, CA. During initial (before the field study) laboratory studies, both instruments were responsive to changes in the simulated aerosol concentration. However, potential problems were discovered involving both instruments during the laboratory-based studies both before and after the Rubidoux study, and these problems are currently being addressed. During the 3-week field study period, 15-minute average particulate nitrate concentrations approaching 30 microg/m3 were observed. Because of malfunctioning IC components (concentrator columns) of the Texas Tech University prototype monitor, limited data were obtained from this instrument during the 3-week sampling period. Initial ambient comparisons show general agreement between the 8400N and IC-based prototype instrument for the semicontinuous determination of ambient particulate nitrate at lower nitrate concentrations (<15 microg/m3) and an underdetermination by the 8400N at higher concentrations (>15 microg/m3). A decreased molybdenum converter efficiency in the pulse analyzer of the 8400N discovered during postfield study laboratory evaluation may explain the negative bias observed at higher nitrate concentrations. Semicontinuous results obtained from U.S. Environmental Protection Agency-operated instruments were averaged and compared with integrated sampler results obtained by Brigham Young University and SCAQMD.