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排序方式: 共有10000条查询结果,搜索用时 15 毫秒
851.
852.
Diana W. Bianchi MD Gretchen K. Zickwolf Melissa C. Yih Alan F. Flint Ossie H. Geifman Marlena S. Erikson John M. Williams 《黑龙江环境通报》1993,13(4):293-300
Fetal nucleated erythrocytes (NRBC) in maternal blood are a non-invasive source of fetal DNA for prenatal genetic screening. We compared the effectiveness of three monoclonal antibodies for the separation of fetal cells from maternal blood by flow sorting. Mononuclear blood cells from 49 healthy pregnant women were incubated with antibody to CD 71, CD 36, and/or glycophorin A (GPA), employed singly or in combination with each other. These monoclonal antibodies recognize surface antigens on haematopoietic precursor cells. Successful isolation of fetal cells was defined as detection of Y chromosomal sequences in maternal blood from women carrying male fetuses, with absence of Y sequences when female fetuses were carried. Thus, gender prediction accuracy was used as a measure of fetal cell separation. Using anti-CD 71 to isolate fetal cells, gender prediction was 57 per cent correct; with anti-CD 36, it was 88 per cent correct. Anti-GPA, an erythrocyte-specific antigen, used alone or in combination with anti-CD 71 or 36, improved gender prediction to 100 per cent. We conclude that antibody to GPA improves the retrieval of fetal NRBC from maternal blood, permitting genetic analysis by the polymerase chain reaction. 相似文献
853.
Lee P. Shulman MD Patricia L. Gordon Donald S. Emerson R. Sidney Wilroy Sherman Elias 《黑龙江环境通报》1993,13(5):403-409
We describe the prenatal diagnosis of isolated bilateral fetal microphthalmia in a woman at increased risk of having a fetus with microphthalmia. Ultrasound examinations at 161 and 19-5 weeks' gestation demonstrated bilateral fetal microphthalmia with no other associated structural defects. The patient elected to terminate her pregnancy at 19.5 weeks. Pathological evaluation of the products of conception obtained by dilation and evacuation confirmed the prenatal diagnosis of isolated bilateral fetal microphthalmia. 相似文献
854.
C. A. Dell'Agnola V. Tomaselli E. Teruzzi B. Tadini A. G. Coran Surgeon-in-Chief 《黑龙江环境通报》1993,13(7):629-632
The impact of prenatal sonographic diagnosis of oesophageal and gastrointestinal obstructions has been analysed over a 10-year period. Three groups of patients were evaluated. The first group consisted of 46 newborns with abnormal prenatal sonogratns, 41 of which were confirmed to have intestinal obstruction postnatally. The second group consisted of 17 neonates with normal prenatal sonograms who had intestinal obstruction postnatally. The third group included 56 newborns who did not undergo a prenatal sonogram but who had intestinal obstruction confirmed at surgery. Polyhydramnios without the appearance of a stomach on ultrasound was diagnostic of pure oesophageal atresia. Polyhydramnios with intestinal dilation was diagnostic of intestinal obstruction. Although surgery was performed earlier in the infants diagnosed prenatally with ultrasound, mortality was no less than in the group that did not undergo a prenatal sonogram, probably because of the high incidence of associated anomalies. 相似文献
855.
As part of the Medical Research Council randomized trial of vitamin supplementation in the prevention of neural tube defects (NTDs), maternal serum alpha-fetoprotein (AFP) was available for 19 NTD pregnancies. Each of these was matched with four unaffected controls, by maternal age, participating centre, and duration of sample storage. The samples came from women whose gestational age ranged from 6 to 14 completed weeks. The median AFP level in the affected pregnancies was 1·2 multiples of the median value in unaffected pregnancies of the same gestational age (95 per cent confidence interval (CI) 0·83–1·59). This confirmed the view that serum AFP measurement is of no practical value in the detection of NTDs in the first trimester of pregnancy. The study also showed that folic acid supplementation, used as a method of preventing NTDs, had no effect on the concentrations of maternal serum AFP up to 14 weeks of pregnancy. 相似文献
856.
E.F. Brünig 《Agriculture, ecosystems & environment》1984,11(2):191
857.
858.
Claus Beier Filip Moldan Richard F.Wright 《Ambio-人类环境杂志》2003,32(4):275-282
到2010年<哥德堡议定书>正式实施时,欧洲自20世纪70年代末以来已减少的酸化硫和氮释放量将进一步减少.这里我们论述了通过使用应用于3个大型"清洁雨"实验(即分别在挪威里斯达尔谢伊阿[Risdalsheia],瑞典耶德松[Gardsjon]以及丹麦克洛斯特海泽[Klosterhede]的所谓"屋顶实验")的酸化模型MAGIC讨论恢复酸化陆地生态系统的结果.<哥德堡议定书>的实施将启动通过重建盐基饱和恢复上述3处土壤的进程.恢复率较低,在今后30年中,盐基饱和增加不足5%.气候引发的风暴严重性的加剧将增加海盐输入生态系统的量.这将为土壤提供额外的碱阳离子,并使恢复速度翻一番,同时还将随着沉积的碱阳离子与存储在土壤中的酸性交换导致高海盐输入之后的强酸性脉动.因此,酸化集水区土壤和径流的进一步恢复将取决于酸沉积下降的数量和速率,在近岸系统的情况下,还取决于海盐输入事件的频率和强度. 相似文献
859.
Stanislav N. Gorb 《Die Naturwissenschaften》1999,86(11):552-555
Two main types of joints occur in the damselfly wing: mobile and immobile. Some longitudinal veins (RP2–, RP3&4–, and MP–) are elastically joined with cross veins, whereas other longitudinal veins (IR1+, IR2+, MA+, CuA'+) are firmly joined with cross veins. In this study we mapped the distribution of serial elastic elements in the wing. The
occurrence of resilin, a rubberlike protein, in mobile joints suggests that the automatic twisting mechanism of the leading
edge by aerodynamic force works not by flexibility but by the elasticity of these joints. First, it should result in elastic
energy storage in the distal areas of the wing. Second, serial elastic elements of wing presumably act as dampers of an aerodynamic
force, which are responsible for gradual twisting of the leading edge.
Received: 16 March 1999 / Accepted in revised form: 26 August 1999 相似文献
860.
While true mosaicism occurs in only 0–25 per cent of genetic amniocenteses, nearly 2–5 per cent of amniotic fluid cell cultures contain a second cell line. In the common practice of prenatal diagnosis, an aberrant cell line confined to a single colony is usually disregarded. We present a case of mosaic trisomy 14 which was not detected on initial chromosome analysis. At birth, multiple malformations were apparent. Newborn cytogenetic studies revealed mosaicism [46,XX/46,XX,-14,+i(14q)] with an isochromosome 14 in 37 per cent of lymphocytes. Additional cells from the initial amniotic fluid culture were analysed post-delivery and the isochromosome 14 identified in only one of 12 total colonies. This case illustrates two important lessons in prenatal diagnosis. First, amniotic fluid cell cultures may not accurately reflect the relative distribution of the normal and abnormal cell lines within a mosaic fetus. Second, while it is generally reasonable to disregard mosaicism confined to a single colony, this policy will, on rare occasion, result in diagnostic error. This should be taken into consideration, particularly when dealing with autosomal trisomies potentially compatible with livebirth. 相似文献