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101.
C. T. Achuthankutty Y. Shrivastava G. G. Mahambre S. C. Goswami M. Madhupratap 《Marine Biology》2000,137(1):19-22
Effect of salinity on the feeding rate and parthenogenetic reproduction of asexual females of the cladoceran Diaphanosoma celebensis Stingelin was studied. Short-term (10 h) grazing experiments were conducted using Isochrysis galbana as feed at 5, 17, 25 and 30 psu salinity. Gut pigment concentration showed a significantly higher rate of feeding at lower
salinities. Survival, growth, maturity attainment and neonate production of asexual females reared in the above four test
salinities indicated preference for lower salinities (5 and 17 psu). The mean size of adult females decreased from 909 to
593 μm, mean life span from 24 to 5 d, mean neonate production from 12 to 2 and mean size of neonates from 434 to 400 μm as
the salinity increased from 5 to 30 psu. Salinity variations also affected the size and age of primiparous females. Resting
egg formation and sexual reproduction did not occur at the tested salinities. The results indicate that D. celebensis is adapted to low saline, estuarine environments.
Received: 14 January 2000 / Accepted: 24 March 2000 相似文献
102.
L. Zane L. Ostellari L. Maccatrozzo L. Bargelloni J. Cuzin-Roudy F. Buchholz T. Patarnello 《Marine Biology》2000,136(2):191-199
Meganyctiphanes norvegica (M. Sars)is a pelagic crustacean that plays a key role in marine food webs of North Atlantic Ocean and marginal seas. We
studied eight population samples collected in the European Atlantic and Mediterranean Sea. By means of single strand conformation
polymorphism analysis (SSCP) and direct sequencing, we investigated a segment of 158 base pairs of the mitochondrial gene
coding for the subunit 1 of NADH dehydrogenase. We found 12 sequence variants among the 385 individuals studied. Analysis
of molecular variance (AMOVA) showed that 14.75% of the total genetic variability was explained by differences between populations,
thus indicating absence of panmixia for these populations. Pairwise comparisons revealed three distinct genetic pools: the
first one represented by Cadiz Bay, the second one by the Ligurian Sea, and the third one included all the NE Atlantic samples.
We also investigated one population from the Alboran Sea (within the Mediterranean basin, east of the Strait of Gibraltar).
This population was found to be genetically intermediate between the NE Atlantic samples and the Ligurian sample, suggesting
that the restriction to the gene flow is not associated with the Strait of Gibraltar, but possibly with the Oran–Almeria oceanographic
front. The present work indicates that M. norvegica, although endowed with a high dispersal capacity because of its pelagic habit, can develop separate breeding units inside
the same oceanic basin (the Atlantic). Furthermore, the Ligurian sample should be considered as a distinct evolutionary entity,
separated from the Atlantic population.
Received: 2 May 1999 / Accepted: 26 November 1999 相似文献
103.
The pathway for the flow of salt-marsh grass production into marsh food-webs is still not well defined. We compared the abilities
of three marsh macroinvertebrates [salt marsh periwinkles, Littoraria irrorata (Say) (=Littorina irrorata), salt-marsh coffee-bean snails, Melampus bidentatus (Say); and a talitrid amphipod, Uhlorchestia spartinophila Bounsfield and Heard] to access standing-dead leaves of smooth cordgrass (Spartina alterniflora Loisel). The invertebrates were incubated with naturally-decaying leaves, and the rates of removal of organic matter and
living fungal biomass (ergosterol) were measured. The impact of invertebrate activity upon fungal growth rates was measured
as rates of fungal-membrane synthesis (incorporation of radioacetate into ergosterol). The removal rates of organic leaf biomass
per mg individual biomass were highest for amphipods (700 μg mg−1 d−1) and lowest for periwinkles (90 μg mg−1 d−1), but the relatively large biomass of the snails made their removal rates per individual greater than those of amphipods.
Net removal of ergosterol by all three invertebrates was >50% for yellow-brown (early-decay) leaf blades. For fully-brown
(advanced-decay) blades, >50% removal of ergosterol was found only for periwinkles; exposure to coffee-bean snails and amphipods
resulted in a net ergosterol reduction of ≤20%. The lower net reduction of living fungal biomass by coffee-bean snails and
amphipods may have been due to fungal-growth stimulation (2.3-fold stimulation in coffee-bean snails and 1.5-fold stimulation
in amphipods). Grazing by periwinkles did not stimulate fungal growth, possibly because of its high intensity. Grazing by
these three salt-marsh shredders may affect marsh-grass shoot-decay in different ways. Periwinkles may abbreviate the period
of fungal production, and incorporate the decaying material relatively quickly into snail biomass and fecal-pellet rain to
the sediments. Coffee-bean snails and amphipods may enhance and prolong fungal production, along with the formation of fecal-pellet
rain. All three invertebrates fed preferentially on leaf blades rather than leaf sheaths, and feeding rates of gastropods
were higher during the night than during the day.
Received: 25 November 1998 / Accepted: 4 November 1999 相似文献
104.
Genetic variation in 15 Holothuria (Microthele) nobilis (Selenka, 1867) populations on the Great Barrier Reef was studied at seven polymorphic allozyme loci. Although populations
were separated by distances up to 1300 km, there were no apparent restrictions to gene flow (F
ST
values were not significantly different from 0) and the maximum Nei's unbiased genetic distance was 0.003. Populations were
in Hardy–Weinberg equilibrium at all loci. The estimated maximum sexual input [this is the ratio of the number of sexually
produced individuals (N*) to the sample size (N
i
)] and the minimum sexual input [this is the ratio of the number of genotypes (N
go
) over the sample size (N
i
)] were used as estimators for the amount of asexual reproduction. Both parameters suggested that H. nobilis reproduces solely by sexual means (N*:N
i
: = 1; N
go
:N
i
= 0.74 to 1). The allozyme data indicated high gene flow between populations, but the possibility that allozyme frequencies
may not be at equilibrium means that it was not possible to distinguish whether the patterns reflect present-day dispersal
or dispersal that occurred in the past.
Received: 27 March 2000 / Accepted: 29 June 2000 相似文献
105.
An experimental study on recolonization and succession of marine macrobenthos in defaunated sediment 总被引:3,自引:0,他引:3
Hypoxia/anoxia in coastal waters is a world wide problem which often results in mass mortality and defaunation of benthos.
In this study, field experiments were carried out to examine recolonization and succession of macrobenthic infauna in defaunated
sediments, and the time required for recovery from complete defaunation to a stable community. Trays (33 cm length × 25.5 cm
width × 11 cm depth) of defaunated sediment were exposed at the subtidal of a pristine site in subtropical Hong Kong. Temporal
changes of macrobenthic communities in defaunated sediment were analyzed by univariate and multivariate statistics, and compared
with those in undisturbed natural sediment at the same site. Initial colonization of macrobenthos occurred rapidly. A total
of 42 species was found, with an average of 258 animals per tray and 24 species per tray recorded in the first month. Abundance
showed a small peak (496 animals per tray) after 3 months, reached a sharp peak (1154 animals per tray) after 6 months, and
declined thereafter. Species number increased gradually, reached a maximum (68 species per tray) after 9 months, and then
decreased. Recolonization was predominantly contributed by larval settlement rather than adult migration. Temporal changes
in abundance, species number and diversity of the macrobenthic community in defaunated sediment resemble the spatial changes
along a decreasing pollution gradient previously defined by other authors. Results of this experiment suggest that newly available
sediment may allow more species to colonize (or coexist) than sediment pre-occupied by an established community. This is probably
due to less interspecific competition in the former habitat. No significant difference in abundance or species richness was
observed between defaunated and natural sediments after 15 months, suggesting that a stable community had been achieved, although
minor variations in species composition were still discernible between defaunated and natural sediments.
Received: 24 May 1999 / Accepted: 20 October 1999 相似文献
106.
Early development of the Antarctic sea urchin Sterechinus neumayeri was examined under two differ-ent culture regimes: one to simulate development near-bottom (“demersal development”) and the
other to simulate the development of embryos in the water column (“pelagic development”). When embryos of both treatments
reached the hatching blastula stage at 5 d post-fertilization (−1.5 °C), the blastulae that had undergone demersal development
evidenced significant differences (by ANOVA or suitable non-parametric comparison) in the following: a thicker blastoderm
layer (12%, P < 0.001), higher ash-free dry weights (19%, P < 0.01), lower mass-specific respiration rates (50%, P < 0.001), higher incorporation rates of 35S-methionine into protein (23%, P < 0.003), and a differential pattern of protein synthesis. When embryos developed demersally, they remained in the jelly-coat
material released with the eggs at spawning. Quantitative isolation of this jelly-coat material in S. neumayeri demonstrated that it contained a significant amount of organic matter, 115 ng ash-free dry mass per egg, equivalent to 17%
of the egg's initial organic mass. Uptake of external nutrients during embryogenesis may be a significant component of the
physiological energetics of this polar invertebrate by allowing the utilization of jelly-coat material released by a female
during spawning.
Received: 21 April 1999 / Accepted: 5 June 2000 相似文献
107.
Simultaneous hermaphroditism with outcrossing, a previously unconfirmed sexual system in decapod crustaceans, is documented
in the shrimp Lysmata wurdemanni (Gibbes), using time-lapse video observations on mating in pairs of “female-phase” (FP) individuals. Copulations between
FPs resulted in successful spawning and development of embryos. However, female-phase hermaphrodites maintained in isolation
were unable to self-fertilize spawned eggs. All smaller individuals possessed characters typical of caridean males, including
male gonopores, appendices masculinae, ejaculatory ducts, and cincinnuli on the first pleopods. However, the gonad of these
male-phase (MP) individuals was an ovotestis with an undeveloped ovarian portion. FPs, which spawn eggs and incubate embryos,
also had male gonopores and an ovotestis terminating in ejaculatory ducts containing sperm. In FPs, male pleopod characters
were absent or reduced, and a female incubatory character, expanded pleopod flanges, showed a greater relative size. Smaller
size classes of the population were composed only of MPs while larger size classes consisted of both MPs and FPs. “Transitionals,”
individuals with MP characters but an ovotestis containing vitellogenic oocytes, were rare and overlapped completely in size
with FP and larger MP individuals. It is suggested that not all MPs transform into FP hermaphrodites. Social interactions
may mediate MP change into FP hermaphrodites in L. wurdemanni. The social system of L. wurdemanni and other Lysmata spp. may determine the exact form of the sexual system in these species.
Received: 26 October 1997 / Accepted: 21 April 1998 相似文献
108.
The Norway lobster Nephrops norvegicus (L.) inhabits burrows in muddy clay sediments (e.g. on the Swedish west coast), where an autumnal oxygen deficiency in the
bottom water can occur. Our experiments investigated whether the irrigation of the burrows would reflect a behavioural adaptation
to hypoxia, and whether any gender differences of such behaviour exist. Irrigation is performed by the pleopods which may
compensate for a decreasing oxygen tension. Pleopod activity (total number of strokes per sampling time), associated with
oxygen concentration and gender, was studied in N. norvegicus kept in artificial burrows resembling their natural habitat. Male and female lobsters were separately exposed to either normoxia
(70% oxygen saturation) or hypoxia (30% oxygen saturation). A sexual difference in behaviour was found, where females irrigated
the burrow less than males during normoxia. Females showed a significant increase of pleopod activity in hypoxia compared
with normoxic conditions, which was not displayed by the males probably due to the degree of individual variation found. However,
when only males were studied during progressive hypoxia (from 60 to 5% oxygen saturation), following any changes of irrigational
behaviour, a significant increase of accumulated pleopod activity occurred. A major increase of pleopod activity appeared
between 60 and 50% oxygen saturation, below which the activity remained high until a critical point (<10% saturation, 11 °C,
33 psu) where irrigation dropped to a level close to that of normoxic values. Activity sessions during hypoxia were longer
and had a higher stroke rate than during normoxia.
Received: 22 October 1997 / Accepted: 26 February 1998 相似文献
109.
The sibling species, Pseudocalanus moultoni (Frost, 1989) and P. newmani (Frost, 1989), occur sympatrically on Georges Bank. Taxonomic discrimination of the species relies on subtle morphological
characteristics, making routine identification of the species very difficult. DNA sequence variation of two mitochondrial
genes, 16S rRNA and cytochrome oxidase I (COI), reliably discriminated P. moultoni and P.␣newmani. Levels of DNA sequence variation for both genes were consistent with those between species of calanoid copepods. A molecular
systematic protocol (based on allele-specific PCR amplification) was designed from the COI sequences and used to discriminate
females of the two species. The distributions and relative abundances of the two species were mapped for April 1996 based
on samples of 15 to 30 females from 12 collections across Georges Bank. The results of this study indicated that P. moultoni females predominated along the northern flank of Georges Bank, while P. newmani females were common on the southern flank, deeper than the 60-m isobath.
Received: 16 May 1997 / Accepted: 26 April 1998 相似文献
110.
Recovery of benthic communities at Macquarie Island (sub-Antarctic) following a small oil spill 总被引:5,自引:0,他引:5
Intertidal communities at Macquarie Island (sub-Antarctic) were re-evaluated seven years after a small oil spill resulting
from the grounding of the “Nella Dan”, and six years after the first assessment of biological impact. Sampling was conducted
to evaluate community structure in three zones of the exposed rocky shore (upper red, kelp, and lower red) and in samples
of Durvillaea antarctica holdfasts. There were no significant differences between the community structure in oiled and control locations in any of
the three shore zones, but holdfast macrofaunal communities at oiled sites still showed evidence of impact. Holdfast community-structure
in samples from heavily oiled sites showed moderate levels of recovery, with increased abundances of species which were considered
sensitive to the oiling in the first post-impact studies and decreased abundances of opportunistic polychaete and oligochaete
worms. In contrast, samples collected from the moderately oiled location at Secluded Bay showed little evidence of recovery.
Holdfasts at this site were filled with sediment containing traces of diesel oil, and the macrofaunal community was dominated
by opportunistic worms. This study clearly indicates that even small incidents of anthropogenic perturbation can have long-lasting
consequences for marine communities at Macquarie Island.
Received: 23 July 1997 / Accepted: 5 February 1998 相似文献