Summary. The sex pheromone of
Ostrinia orientalis
(Lepidoptera: Crambidae) was analyzed by gas chromatography–electroantennographic detection (GC–EAD), GC–mass spectrometry and a series of bioassays. Three EAD-active
compounds were detected in the female sex pheromone
gland extract, and identified as tetradecyl acetate (14:OAc),
(
Z)-11-tetradecenyl acetate (
Z11-14:OAc) and (
E)-11-tetradecenyl acetate (
E11-14:OAc). The titers (ratio) of 14:OAc,
Z11-14:OAc and
E11-14:OAc in 3-day-old virgin
females were 0.49 ng (10), 4.86 ng (98) and 0.10 ng (2),
respectively. In a wind-tunnel bioassay, the 98:2 blend of
Z11- and
E11-14:OAc, but not
Z11-14:OAc alone, elicited
the same male behavioral responses as virgin females and
crude gland extracts. 14:OAc was inactive by itself, and did
not show any synergistic effect on the binary blend.
Field trapping experiments also confirmed the attractiveness
of the binary blend to
O. orientalis
males. Based on
these results, we concluded that the sex pheromone of
O. orientalis
is a 98:2 mixture of
Z11-14:OAc and
E11-14:OAc. This sex pheromone is very similar to that of the
Z-type European corn borer,
O. nubilalis. The present
finding raises the question of whether
O. orientalis
, which is indistinguishable from
O. nubilalis
based on external morphology,
is a biologically distinct species independent from
O. nubilalis.
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