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31.
In this work, a deterministic and a probabilistic method for the delineation of well head protection areas are applied and compared. The deterministic method was implemented using the automatic backward particle tracking algorithm (APA, Tosco et al., Water Resour Res, 44(7):W07419, 2008). The backward probability model rests upon the backward adjoint-based model developed by Neupauer and Wilson, and allows the inclusion of dispersion in the definition of capture zones. The two methods are evaluated comparing the “advective front” of the probability protection area and the perimeter given by the particle tracking method. Furthermore, a semi-quantitative study was performed over probability protection areas, in order to evaluate the influence of dispersivity on the extent and growth rate of capture zones identified by fixed probability isolines. 相似文献
32.
Grassi Patrícia Drumm Fernanda C. Spannemberg Stéfani S. Georgin Jordana Tonato Denise Mazutti Marcio A. Gonçalves Janaína O. Oliveira Marcos L. S. Dotto Guilherme L. Jahn Sérgio L. 《Environmental science and pollution research international》2020,27(10):10484-10494
Environmental Science and Pollution Research - Sugarcane bagasse, a largely available waste worldwide, was submitted to solid-state fermentation (SSF) using the fungus Metarhizium anisopliae,... 相似文献
33.
Alexandre Domingues Tony F. Grassi Ana L.T. Spinardi-Barbisan Luís F. Barbisan 《Journal of environmental science and health. Part. B》2013,48(5):420-426
This study investigated whether perinatal exposure to diuron [3-(3,4-dichlorophenyl)-1-1-dimethylurea] might exert adverse effects on rat lymphoid organs. Pregnant Sprague-Dawley (SD) rats were exposed to diuron at 500, 750 or 1250 ppm in the diet from gestational days (GD) 12–21 and during lactation. At postnatal day (PND) 42, male pups were euthanized and thymus, spleen, mesenteric lymph node and femur were collected for histopathological analysis. Food consumption and body weight gain were significantly reduced in dams exposed to 1250 ppm during gestation period. Also, Diuron at 750 and 1250 ppm produced: (1) increased relative spleen weight associated histologically with severe congestion in red pulp, (2) enhanced extramedullary hematopoiesis and hemosiderosis as well as (3) depletion of lymphoid follicles in white pulp. Flow cytometric analysis revealed a significant reduction in B lymphocytes (CD45RA+) in male pups but T lymphocytes (CD4+, CD8+ and CD4+/CD8+) were not markedly affected. Thus, data suggest that Diuron-induced maternal toxicity in dams exposed to high dose and perinatal exposure to this herbicide produced spleen toxicity as evidenced by a reduction in B lymphocyte number in male SD pups. 相似文献
34.
Patrizia Stefanelli Tiziana Generali Danilo Attard Barbini Silvana Girolimetti Roberto Dommarco 《Journal of environmental science and health. Part. B》2013,48(7):523-529
In this work we report the results for estimating the measurement uncertainty (MU) following up the application of two different approaches, relatively the top-down procedure, by using proficiency test data. We have focused the estimation on the olive oil matrix. We used the analytical data obtained from five selected editions of the Proficiency Tests (PTs, from 2007 to 2011) on pesticide residues in olive oil to estimate the MU. These PTs have been organized by Istituto Superiore di Sanità annually in cooperation with International Olive Council (IOC) since 1997. The number of participants in each trial ranged from 10 to 43. We used a total of 34 pesticide results. The expanded uncertainty U (c) was calculated using a covering factor k = 2 for a confidence interval of 95%. In the approach 1, the within–laboratory reproducibility standard deviation is combined with estimates of the method and laboratory bias using PTs data. In the approach 2, the way of estimating the MU is based only on the bias that the laboratory has obtained participating in a sufficient number of the IOC proficiency tests. Comparing the relative expanded uncertainty based on these different approaches we notice values quite constant and close, from 42% to 48%. Moreover, these calculated expanded uncertainties are less than the default value of 50% (corresponding to a 95% confidence level), adopted from European guidance document SANCO based on the fit-for-purpose relative standard deviation (FFP-RSD). 相似文献
35.
Luca Alessandria Tiziana Schilirò Raffaella Degan Deborah Traversi Giorgio Gilli 《Environmental science and pollution research international》2014,21(8):5554-5564
Recently, much attention has been devoted to urban air pollution because epidemiological studies have reported health impacts related to particulate matter (PM). PM10 and PM2.5 were collected during different seasons in Torino, a northern Italian city, and were characterised by inorganic chemical species (secondary particulates and bio-available iron). The biological effects of aqueous and organic solvent PM extracts on human epithelial lung A549 were evaluated, and the effects on cell proliferation and lactate dehydrogenase (LDH) release were assayed. The average PM10 concentration during the sampling period was 47.9?±?18.0 μg/m3; the secondary particles accounted for 49 %?±?9 % of the PM10 total mass, and the bio-available iron concentration was 0.067?±?0.045 μg/m3. The PM2.5/PM10 ratio in Torino ranged from 0.47 to 0.90 and was higher in cold months than in warm months. The PM10 and PM2.5 extracts inhibited cell proliferation and induced LDH release in a dose-dependent manner with a seasonal trend. The PM10 extract had a stronger effect on LDH release, whereas the PM2.5 extract more strongly inhibited cell proliferation. No significant differences were observed in the effects induced by the two extracts, and no significant correlations were found between the biological effects and the PM components evaluated in this study, thus emphasising the importance of the entire mixture in inducing a cytotoxic response. 相似文献
36.
Manuela Ciarrocca Assunta Capozzella Francesco Tomei Gianfranco Tomei Tiziana Caciari 《Chemosphere》2013,90(7):2077-2084
ObjectiveThis cross-sectional study was conducted to assess the relationship between exposure to cadmium and circulating reproductive hormone levels in urban and rural male workers.Materials and MethodsUrinary cadmium, blood cadmium, luteinizing hormone (LH), follicle stimulating hormone (FSH) and testosterone were obtained from 86 non-smoking traffic policemen and 86 subjects working as roadmen in a rural area. All subjects were monitored to evaluate airborne exposure to cadmium.ResultsThe mean value of exposure to cadmium was 1.3 ng m?3 in traffic policemen, while the mean value was less than 0.5 ng m?3 in roadmen. The mean concentrations of urinary cadmium (1.4 vs. 0.9 μg g?1 creatinine; p = 0.001), blood cadmium (1.1 vs. 0.7 μg l?1; p = 0.000), FSH (2.6 vs. 3.2 μlU ml?1; p = 0.02) and LH (2.6 vs. 3.1 μlU ml?1; p = 0.03) were significantly different between traffic policemen and roadmen. No differences were found in the mean values of testosterone between the two groups. Multiple linear regression models showed associations between (a) urinary cadmium, airborne cadmium, working life, job category and consumption of water from water supply (b) blood cadmium, airborne cadmium and job category (c) the values of FSH and age, working life, job category, urinary cadmium and blood cadmium (d) the values of LH and both the age and working life.ConclusionThe above results must be confirmed by further studies, but they indicate the influence of exposure to the cadmium present in urban air on the circulating FSH, even at low doses. 相似文献
37.
Pignata C Fea E Rovere R Degan R Lorenzi E de Ceglia M Schilirò T Gilli G 《Environmental monitoring and assessment》2012,184(4):2091-2103
This study investigates the impact of wastewater treatment plant (WWTP) effluent on the toxicity of the recipient water body
and the effectiveness of the disinfection treatment applied (sodium hypochloride) to assure the compliance of both microbiological
and toxicological emission limits. No toxicity was found in the majority of samples collected from the recipient river, upstream
and downstream of the WWTP, using three different toxicity tests (Vibrio fischeri, Daphnia magna, and Pseudokirchneriella subcapitata). Only three samples presented toxic unit (TU) values with V. fischeri, and one presented TU with P. subcapitata. The influent toxicity ranged from slightly toxic to toxic (TU = 0.68–4.47) with V. fischeri, while only three samples presented TU values with the other tests. No toxicity was found in the absence of chlorination,
while the mean toxicity was 3.42 ± 4.12 TU with chlorination in the effluent. Although no toxicity or very slight toxicity
was found in the receiving water, its residual toxicity was higher than the US EPA Quality Standard in two samples. Escherichia coli concentration had a lower mean value in the chlorinated effluent: 13,993 ± 12,037 CFU/100 mL vs. 62,857 ± 80,526 CFU/100 mL
for the not chlorinated effluent. This difference was shown to be significant (p < 0.05). E. coli in ten chlorinated samples was higher than the limit established by European and Italian Legislation. The mean highest trihalomethanes
(THMs) value was found in the influent samples (2.79 ± 1.40 μg/L), while the mean highest disinfection by-products (DBPs)
was found in the effluent samples (1.85 ± 2.25 μg/L). Significant correlations were found between toxicity, sodium hypochlorite,
THMs, DBPs, E. coli, and residual chlorine. In conclusion, this study highlighted that the disinfection of wastewater effluents with sodium hypochlorite
determines the increase of the toxicity, and sometimes is not enough to control the E. coli contamination. 相似文献
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Tiziana Romagnoli Giorgio Bavestrello Emellina M. Cucchiari Mario De Stefano Cristina G. Di Camillo Chiara Pennesi Stefania Puce Cecilia Totti 《Marine Biology》2007,151(2):537-552
The microalgal community associated with Eudendrium racemosum, a marine hydroid widely distributed in the Mediterranean Sea, was studied during an annual cycle, at monthly frequency,
in a coastal station of the Ligurian Sea. Microalgae were represented mainly by diatoms, which exhibited higher abundance
and biomass values between autumn and spring (max 46,752 cells mm−2 and 1.94 μg C mm−2, respectively), while during summer a significant decrease was observed (min 917 cells mm−2 and 0.013 μg C mm−2). High levels of abundance of filamentous cyanobacteria were observed in summer. Spatial distribution of epibiontic microalgae
showed a markedly increasing gradient from the basal to the apical part of hydroid colonies. Considering the growth forms
of diatom communities, motile diatoms (mainly small naviculoid taxa) were the most abundant in all the periods. Adnate (Amphora and Cocconeis spp.) were distributed mainly in the basal and central part of hydroid colonies and showed two peaks (autumn and summer).
Erect forms (mainly Tabularia tabulata, Licmophora spp., Cyclophora tenuis) were mainly distributed in the apical part of the colonies and showed their maximum densities in spring–summer. Tube-dwelling
(Berkeleya rutilans, Parlibellus sp.) were observed at low densities throughout the study period, without any significant temporal or spatial variability.
Comparing the microalgal communities on marine hydroid to those grown on mimic substrata placed in the sampling station during
summer, significantly higher values of abundance were observed in the hydroid, suggesting that microalgae may benefit from
the polyp catabolites. This fact was particularly evident for the adnate diatoms, whose temporal trend paralleled the cycle
of hydroid host. 相似文献
40.
Labra M Gianazza E Waitt R Eberini I Sozzi A Regondi S Grassi F Agradi E 《Chemosphere》2006,62(8):1234-1244
The plant metabolic response to heavy metal stress is largely unknown. The present investigation was undertaken to examine the influence of different concentrations of potassium dichromate on the Zea mays L. plantlets. A clear effect of chromium on maize plantlets growth and seed germination was observed strating from 100-300 ppm up to 1500 ppm. In this concentration range, chromium uptake was dependent on the concentration in the medium. Metallothioneins, involved in heavy metal binding, were measured by capillary electrophoresis (CE), and showed a dose-response induction. Protein profile analyzed by two-dimensional gel electrophoresis showed differential expression of several proteins. Identification of spots of upregulated proteins was performed by MALDI mass spectrometry. Results showed that proteins induced by heavy metal exposure are principally involved in oxidative stress tolerance or in other stress pathways. Induction of proteins implicated in sugar metabolism was also observed. Identification of factors involved in plant response may lead to a better understanding of the mechanisms involved in cell protection and tolerance. This information could be used to improve agricultural production and environmental quality. 相似文献