Activities of metabolic enzymes in the deep-water crabsChaceon fenneri andC. quinquedens and the shallow-water crabCallinectes sapidus

The activities of 18 enzymes were measured in gill, hepatopancreas and muscle tissue of the deep-water crabsChaceon fenneri andC. quinquedens and the shallow-water crabCallinectes sapidus collected from the Gulf of Mexico in January 1989. The activities of catabolic enzymes were correlated in general with the known metabolic rates of the three species. Activities were much higher inC. sapidus than inChaceon fenneri andC. quinquedens. In some cases,C. quinquedens had higher activities thanC. fenneri. The activities of enzymes of amino acid metabolism (glutamate dehydrogenase and alanine aminotransferase) were higher inC. quinquedens, which had high hemolymph [ammonia] and ammonia excretion rates. The activity of lactate dehydrogenase (LDH) ofC. fenneri andC. quinquedens were correlated with the two species' abilities to withstand hypoxia. The more hypoxiatolerant species,C. quinquedens, had higher activity of LDH in its muscles than didC. fenneri.     

Habitat use,urea production and spawning in the gulf toadfish <Emphasis Type="Italic">Opsanus beta</Emphasis>

Field studies were conducted in Johnson Key Basin, Florida Bay, USA from September 2002 through September 2004 to examine physiological, ecological, and behavioral characteristics of the gulf toadfish, Opsanus beta (Goode and Bean in Proc US Natl Mes 3:333–345, 1880), in relation to nitrogen metabolism, habitat usage, and spawning. Fish collected 5 cm above sediments in experimental shelters (epibenthic) were compared with those collected by throw traps which were found on or burrowing within sediments. The relationship between microhabitat ammonia and urea excretion, as determined by the enzymatic activity of glutamine synthetase (GS), was examined. The hypothesis tested was that O. beta occupying epibenthic nests were less ureotelic with lower GS activities than non-nesting individuals on/in sediments, due to a decreased environmental ammonia burden. Porewater total ammonia (T _Amm) concentrations at a sediment depth of 5 cm, i.e., the approximate depth of burrowing toadfish, ranged from 0 to 106.5 μmol N l⁻¹ while the pH ranged from 7.48 to 9.14. There was a weak but significant correlation between environmental ammonia (NH₃) concentration and hepatic GS activity for epibenthic toadfish (P < 0.001, r ² = 0.10), but not for burrowing toadfish. Mean urea-N and T _Amm concentrations within shelters occupied by toadfish (n = 281) were 9.8 ± 0.83 μmol N l⁻¹ and 13.0 ± 0.7 μmol N l⁻¹, respectively. As predicted, hepatic GS activity was significantly lower in epibenthic toadfish captured in shelters (4.40 ± 0.24 μmol min⁻¹ g⁻¹; n = 281) as compared to individuals on/in sediments (6.61 ± 0.47 μmol min⁻¹ g⁻¹; n = 128). Glutamine synthetase activity generally peaked in March (spawning season) and was lowest in July. Gender differences in hepatic and branchial GS activity were also found during the spawning season, which is attributable to the fact that males brood and guard offspring in their epibenthic nests while females often rest on or burrow into the sediments. Finally, hepatic and branchial GS appeared to have different patterns of enzymatic activity suggesting functional differences in gene expression.     

General equilibrium benefits for environmental improvements: projected ozone reductions under EPA's Prospective Analysis for the Los Angeles air basin

This research demonstrates how locational equilibrium models can be used for benefit measurement with the detail required to match EPA's benefit analysis for the first Prospective Analysis. Using the projected changes in ozone concentrations for 2000 and 2010 together with the Sieg et al. (Int. Econ. Rev., forthcoming) estimates for household preferences for housing, education, and air quality, this paper measures general equilibrium willingness to pay for the policy scenarios developed for the Prospective study as they relate to households in the Los Angeles area. Benefits are evaluated taking account (at the household level) of initial air quality conditions, relocation based on changes in ozone, and price changes. The framework generalizes the partial equilibrium/general equilibrium comparisons available with conventional computable general equilibrium and property capitalization models. Estimated general equilibrium gains from the policy range from $33 to $2400 annually at a household level (in 1990 dollars).     

Health and safety implications of alternative energy technologies. III. Fossil energy

This paper reviews both innovative fossil energy sources (tar sands, oil shale, and unconventional natural gas), and more established technologies that are being considered as suppliers of gaseous and liquid fuels (that is, coal gasification and liquefaction). Potential health and safety issues related to the technologies are discussed, although the absence of commercial-scale facilities in the United States restricts the discussion to health effects information derived from related processes. The available epidemiological and carcinogenic studies give cause for concern. The study of the health and environmental impacts of the emerging fossil fuel technologies will be important for quantification of adverse effects and rectification of problems before commitment to large-scale commercialization occurs.Operated by Union Carbide Corporation under contract W-7405-eng-26 with the U.S. Department of Energy.     

RDX and TNT residues from live-fire and blow-in-place detonations

Snow was used as a collection medium to examine 1,3,5-hexahydro-1,3,5-trinitrotriazine (RDX) and 2,4,6-trinitrotoluene (TNT) residues post-detonation of 60-, 81-, and 120-mm mortar rounds, 105- and 155-mm howitzer rounds, M67 hand grenades, 40-mm rifle grenades, and blocks of C4. Residue-covered snow samples were collected, processed, and analyzed for explosives without cross-contamination from previous detonations and other potential matrix interferences. Detonation trials were performed following standard military live-fire and blow-in-place techniques. When possible, replicate munitions were detonated under similar conditions to provide a more reliable estimation of the mass of unconsumed high explosive residues. Overall the amount of energetic residues deposited from live-fire detonations were considerably less than the energetic residues deposited by blow-in-place detonations.     

More than money: how multiple factors influence householder participation in at-source stormwater management

Urban stormwater run-off is a threat to stream ecosystems. New approaches to stormwater management aim to protect urban streams from such impacts, by retaining, treating and using stormwater at its source. As up to ～50% of runoff from urban surfaces comes from private property, fostering stormwater retention requires effective householder engagement. We evaluated householder participation in the Little Stringybark Creek project, a stormwater retrofit programme aimed at waterway protection, using qualitative enquiry through formal and informal interviews to identify factors that influenced participation. Participation was governed by multiple factors, with financial incentives and personal co-benefits of tanks primary motivators, while process complexity and distrust were primary barriers. Results suggest an approach combining education to encourage review of subjective norms and attitudes, with incentives to mitigate behavioural controls can transform public behaviour towards sustainable stormwater management.     

The Clean Air Act Amendments and Motor Vehicle Inspection/Maintenance

Seventeen days of detailed measurements of NO, NO₂, O₃, HNO₃ and the frequency of NO₂ photolysis—j(NO₂)—were carried out in Claremont, CA, in September 1980. Under conditions when the rate of change of NO concentration is small, there must be a balance between formation and loss processes. In the classical photostationary state this balance is between NO₂ photolysis: NO₂ + hv → NO + O and reaction with ozone: NO + O₃ → NO₂ + O₂. The results show that the latter reaction with ozone is inadequate to balance the formation step; a significant contribution is required from another NO oxidation process, possibly peroxy radical oxidation. If so, the inferred concentration of peroxy radicals shows a diurnal variation, peaking around solar noon.     

Optimization of a horizontal-flow biofilm reactor for the removal of methane at low temperatures

Three pilot-scale, horizontal-flow biofilm reactors (HFBRs 1–3) were used to treat methane (CH₄)-contaminated air to assess the potential of this technology to manage emissions from agricultural activities, waste and wastewater treatment facilities, and landfills. The study was conducted over two phases (Phase 1, lasting 90 days and Phase 2, lasting 45 days). The reactors were operated at 10 °C (typical of ambient air and wastewater temperatures in northern Europe), and were simultaneously dosed with CH₄-contaminated air and a synthetic wastewater (SWW). The influent loading rates to the reactors were 8.6 g CH₄/m³/hr (4.3 g CH₄/m² TPSA/hr; where TPSA is top plan surface area). Despite the low operating temperatures, an overall average removal of 4.63 g CH₄/m³/day was observed during Phase 2. The maximum removal efficiency (RE) for the trial was 88%. Potential (maximum) rates of methane oxidation were measured and indicated that biofilm samples taken from various regions in the HFBRs had mostly equal CH₄ removal potential. In situ activity rates were dependent on which part of the reactor samples were obtained. The results indicate the potential of the HFBR, a simple and robust technology, to biologically treat CH₄ emissions.

Implications: The results of this study indicate that the HFBR technology could be effectively applied to the reduction of greenhouse gas emissions from wastewater treatment plants and agricultural facilities at lower temperatures common to northern Europe. This could reduce the carbon footprint of waste treatment and agricultural livestock facilities. Activity tests indicate that methanotrophic communities can be supported at these temperatures. Furthermore, these data can lead to improved reactor design and optimization by allowing conditions to be engineered to allow for improved removal rates, particularly at lower temperatures. The technology is simple to construct and operate, and with some optimization of the liquid phase to improve mass transfer, the HFBR represents a viable, cost-effective solution for these emissions.     

Manipulating decay time for efficient large-mammal density estimation: gorillas and dung height

Large-mammal surveys often rely on indirect signs such as dung or nests. Sign density is usually translated into animal density using sign production and decay rates. In principle, such auxiliary variable estimates should be made in a spatially unbiased manner. However, traditional decay rate estimation methods entail following many signs from production to disappearance, which, in large study areas, requires extensive travel effort. Consequently, decay rate estimates have tended to be made instead at some convenient but unrepresentative location. In this study we evaluated how much bias might be induced by extrapolating decay rates from unrepresentative locations, how much effort would be required to implement current methods in a spatially unbiased manner, and what alternate approaches might be used to improve precision. To evaluate the extent of bias induced by unrepresentative sampling, we collected data on gorilla dung at several central African sites. Variation in gorilla dung decay rate was enormous, varying by up to an order of magnitude within and between survey zones. We then estimated what the effort-precision relationship would be for a previously suggested "retrospective" decay rate (RDR) method, if it were implemented in a spatially unbiased manner. We also evaluated precision for a marked sign count (MSC) approach that does not use a decay rate. Because they require repeat visits to remote locations, both RDR and MSC require enormous effort levels in order to gain precise density estimates. Finally, we examined an objective criterion for decay (i.e., dung height). This showed great potential for improving RDR efficiency because choosing a high threshold height for decay reduces decay time and, consequently, the number of visits that need to be made to remote areas. The ability to adjust decay time using an objective decay criterion also opens up the potential for a "prospective" decay rate (PDR) approach. Further research is necessary to evaluate whether the temporal bias inherent in such an approach is small enough to ignore, given the 10-20-fold increases in precision promised by a PDR approach.