Isolation,identification, and the degradation characteristics of a BDE-47 degrading strain北大核心CSCD

In an effort to remove BDE-47 residues from the environment, a bacterial strain that is capable of utilizing BDE-47 as the sole carbon source was isolated and screened from soil collected from an e-waste recycling area in Tianjin to analyze the degradation properties. The strain was preliminarily identified as Enterobacter sp. according to a 16S rDNA gene sequence analysis. The strain degraded 35.8% of 525 μg/L of BDE-47 in 35 d when the initial concentration of bacteria was 7.1 × 105 cells/ mL. The product of the biodegradation of BDE-47 was BDE-28. The biodegradation of BDE-47 fit well with first-order kinetics, and its degradation kinetics was ln Ct = - 0.104t + 6.22. With the addition of an electron acceptor, such as Fe3+, SO4 2- and NO3 -, the BDE-47 degradation rate was significantly increased to 49.8%, 59.1%, and 67.3%, respectively. The above results revealed that the strain could degrade BDE-47, which is of importance in the application of environmental bioremediation of BDE-47. © 2018 Science Press. All rights reserved.     

Mapping loci that control lateral spike development in Tibetan barley by bulked segregant analysis (BSA)北大核心CSCD

Fertility of lateral spikelets determines the two-rowed or six-rowed spikes of barley (Hordeum vulgare L.), which results in significantly different grain yields. The change in row type from two-rowed to six-rowed shows remarkable domestication characteristics. The Qinghai-Tibet plateau has abundant resources of wild and cultivated barley, and is considered one of the centers of domestication and genetic diversity of cultivated barley. In order to obtain a primary understanding of the genetic basis of lateral spike development regulation and the domestication process in cultivated Tibetan barley, an F2 segregation population was constructed by crossing the two-rowed wild barley accession ZYM0083 with the six-rowed landraces Linzhiheiliuleng. Genetic analysis showed that the row type was controlled by a single gene. Using the specific-locus amplified fragment sequencing (SLAF-seq) technology and bulked segregant analysis (BSA), two DNA pools from 22 two-rowed spike individuals and 22 six-rowed spike individuals of the F2 population were constructed and sequenced. A total of 456 691 SLAF tags were obtained. By adopting the ED and SNP index for association analysis, three candidate regions with a 53.84-Mb interval and containing 536 genes were obtained. Four-hundred thirteen, 189, and 160 annotated genes were acquired by GO, KEGG, and COG libraries, respectively. Loci that control lateral spike development in Tibetan barley were primarily mapped by SLAF-seq, and the results presented in this study will facilitate the fine mapping and cloning of target genes. © 2018 Science Press. All rights reserved.     

Characteristics of culturable endophytic bacteria of Xanthoceras sorbifolia Bunge with tumor roots北大核心CSCD

Xanthoceras sorbifolia Bunge with tumor roots was discovered, and the endophytic bacteria that were isolated from the tumor roots were purified and identified. This paper aimed to study the characteristics of endophytic bacteria. The CAS detection plate, Salkowski colorimetry, phosphate solubilizing circle, and molybdenum antimony spectrophotometry were used to analyze endophytic bacteria ability, which produced siderophores, secreted indole-3-acetic acid (IAA), and dissolved phosphorus. Strains were isolated from the tumor roots through morphological and molecular identification, and they were named XSB1-XSB9, of which 6 strains belonged to Bacillus sp., 2 strains belonged to Brevibacillus sp., and 1 strain belonged to Pseudomonas sp. All 9 strains produced siderophores; strains XSB3, XSB4, XSB8, and XSB9 were extremely high yielding, and strains XSB5 and XSB6 were high yielding. The strains with high yields were XSB3, XSB4, XSB5, XSB6, XSB8, and XSB9 and accounted for 66.7% of the tested strains. Nine strains secreted IAA; the concentration of IAA secreted by the strains that contained tryptophan was between 15-50 mg/L, and the concentration of IAA secreted by the one strain without tryptophan was between 10-35 mg/L. The IAA ability of the XSB2, XSB3, XSB4, XSB5, and XSB9 strains were significantly different (P < 0.05) after adding tryptophan. These results indicate that the synthesis of IAA may be the tryptophan synthesis pathway where tryptophan is used as a precursor. All 9 strains had some ability to dissolve phosphorus. The concentration of phosphate in the solubilizing strains, XSB1, XSB2, XSB4, and XSB5, were significantly higher than that in the other strains (P < 0.01), and the concentration of phosphate solubilizing was between 50-90 mg/L; the ability to dissolve phosphate was increased by 19-29 times. The XSB4 and XSB5 strains, which produced siderophores, secreted IAA and had a strong capacity to dissolve phosphorus, may be candidate strains for promoting growth. This research provides the foundation for the development and utilization of rhizosphere microbes to understand the resistance mechanisms and cultivation level of X. sorbifolia. © 2018 Science Press. All rights reserved.     

Fine root decomposition and nutrient release in Toona sinensis plantation under the reconstruction mode of low-efficiency cypress forest gaps北大核心CSCD

Fine root decomposition is an important way in which nutrients are returned to plantation soil; thus, further study of this process will be helpful for understanding material cycling in forest ecosystems. We investigated a Toona sinensis plantation in the central Sichuan hilly region using litter bags containing T. sinensis fine roots to evaluate the dynamics of fine root decomposition and nutrient release for one year in forest gaps of 50 m2 (L1), 100 m2 (L2), and 150 m2 (L3). The results showed that T. sinensis fine root decomposition was fastest in the first 90 days. As time passed, the decomposition rate slowed. One year later, the residue rate was 75.44%, 73.92%, and 72.07%, respectively. The fine root decomposition rate of L3 was greater than that of L2, which was greater than that of L1. During fine root decomposition, the dynamics of the fine root nutrient concentrations changed. C, P, and K concentrations of the fine roots declined in forest gaps, while N, Ca, and Mg concentrations increased overall in the fine roots. In conclusion, forest gaps had effects on the fine root decomposition and nutrient release of T. sinensis, and different sized forest gaps produced different results. © 2018 Science Press. All rights reserved.     

Effects of a lead- and cadmium-resistant fungal strain on the absorption of heavy metals by Vetiveria zizanioides北大核心CSCD

A two-factor randomized complete block experiment was used to explore the remediation by a plant-microorganism combination on soils contaminated by lead (Pb) and cadmium (Cd). Factor A was the amount of fungi, for which four values were considered, namely, 0, 1, 3, and 5 g. Factor B was the level of contamination by lead and cadmium, for which six values were considered, namely, Cd0Pb0, Cd10Pb400, Cd20Pb600, Cd30Pb800, Cd50Pb1200, and Cd80Pb1800 (data in units of mg/ kg). The results showed that the resistant fungi promoted the growth of vetiver (Vetiveria zizanioides). At weights of 1, 3, and 5 g, the resistant fungi increased the biomass of vetiver by 41.9%, 74.9%, and 71.7% respectively. The resistant fungi stimulated the absorption of lead and cadmium by both the aerial and underground parts of vetiver. In the presence of 80 mg/kg of Cd2+ and 1 800 mg/kg of Pb2+, the contents of lead in the aerial parts of vetiver were increased by 120.6%, 265.4%, and 242.9%, while the lead content in the underground parts were increased by 110.3%, 278.2%, and 266.2%, after the addition of 1 g, 3 g, and 5 g of fungi, respectively. The content of cadmium in the aerial parts increased by 113.2%, 238.3%, and 217.3%, while the content of cadmium in the underground parts increased by 103.1%, 298.8%, and 274.4%, after the addition of 1 g, 3 g, and 5 g of fungi, respectively. The addition of fungi strengthened the effect of V. zizanioides to remediate soils contaminated by lead and cadmium, and the remediation after the addition of 3 g of fungi was better than that after treatment with 1 g and 5 g of fungi. The combination of resistant fungi and the heavy metal enrichment plant, vetiver, under different concentrations of lead and cadmium showed that the fungi had a significant effect on the remediation of soils contaminated by lead and cadmium. © 2018 Science Press. All rights reserved.     

铁循环微生物对环境中重金属的影响研究进展

铁循环微生物包括铁氧化菌(Fe(Ⅱ)-oxidizing bacteria,FeOB)和铁还原菌(Fe(Ⅲ)-reducing bacteria,FeRB),在由它们介导Fe²⁺(Fe³⁺)氧化(还原)的过程中,往往也伴随着一系列重金属元素的迁移转化,对重金属在环境中的生物有效性和迁移性方面有重要作用.本文综述了环境中的铁循环微生物,针对铁循环微生物驱动重金属迁移转化的作用机制,分别从铁氧化菌氧化亚铁生成铁矿物对重金属的固定,铁还原菌介导铁矿物还原溶解及次生矿物生成,以及铁循环微生物代谢耦合重金属形态转化方面进行阐述;进一步通过研究实例综述了铁循环微生物对环境中砷、镉、铬、铜、铅等重金属的作用及修复潜力;未来的研究可关注特定微生物的成矿机制,生物成矿对重金属固定的调控,以及重金属复合污染场地的铁循环微生物修复应用等方面.本文以期为基于铁循环微生物的重金属污染修复提供理论指导和应用依据.     

基于数值模拟的海湾环境容量价值损失的预测评估 ——以厦门同安湾围填海为例

基于海湾环境容量价值影响因素的分析,采用环境经济学的影子工程法,结合水动力数值模型及其与污染物扩散的耦合模型,建立了围填海导致的海湾环境容量价值损失的预测评估模型和方法,并以厦门同安湾为例,对同安湾4个围填海规划方案可能造成的环境容量价值的损失进行了预测评估.结果表明,方案1~方案4围填海面积依次为1.98,7.73,9.38,19.24km2,围填海造成的环境容量的损失依次为12281.3,48418.6,60706.6,132800.1万元/a,单位面积的损失依次为62.03,62.64,64.72,69.02元/(m2×a).围填海活动带来的环境成本不容忽视.基于评估结果,针对厦门围填海的现状提出了相关的政策建议.     

添加低比例石灰调质的脱水污泥堆肥试验研究

添加石灰可以快速实现污泥干化,抑制污泥恶臭产生、钝化重金属及杀灭病原微生物,但大量石灰(>5%)的加入不但会增加成本,而且会明显提高产物pH值,极大限制了其后续利用,因此,开展了采用添加低比例(￡5%)石灰调质进行污泥堆肥的研究.试验采用罗迪格(Leodige)高效混合设备制备石灰质量分数分别为1%、5%和5%+熟料的混合污泥作为堆肥原料,与未添加石灰的污泥进行对比堆肥.采用氧温控制系统在线监测氧气和温度,实时反馈并控制系统通风.结果表明:堆肥15d后,添加石灰的3组堆肥pH值分别从9.06、12.17、12.34下降至弱碱性水平(<8.3),挥发分从57.35%、45.97%、44.59%下降至44.20%、39.28%、38.42%;4组堆肥减量比均达到50%以上,除2#减量速率明显较慢外,其他3组堆肥减量速率无显著差异;重金属浸出试验检测发现,重金属浸出浓度受pH值的影响较大,添加5%石灰的堆体,Cu、Ni、Zn的浸出液浓度最低.工程应用中,建议采用添加质量分数5%的石灰与一定的熟料返混,从而提高堆肥效率及产品品质.