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261.
Altamira Cave contains valuable paleolithic paintings dating back to 15,000 years. The conservation of these unique paintings
is attracting increasing interest, and so, understanding microbial proliferation in Altamira Cave represents a prioritary
objective. Here, we show for the first time that members of the Crenarchaeota were metabolically active components of developing
microbial communities. RNA was extracted directly from the studied environment, and a number of 16S rRNA gene sequences belonging
to the low-temperature Crenarchaeota were detected. Although low-temperature Crenarchaeota detected in a variety of ecosystems
by using molecular techniques remain uncultured, this RNA-based study confirms an active participation of the Crenarchaeota
in cave biogeochemical cycles. 相似文献
262.
Alastuey A Querol X Plana F Viana M Ruiz CR Sánchez de la Campa A de la Rosa J Mantilla E García dos Santos S 《Journal of the Air & Waste Management Association (1995)》2006,56(7):993-1006
A detailed physical and chemical characterization of coarse particulate matter (PM10) and fine particulate matter (PM2.5) in the city of Huelva (in Southwestern Spain) was carried out during 2001 and 2002. To identify the major emission sources with a significant influence on PM10 and PM2.5, a methodology was developed based on the combination of: (1) real-time measurements of levels of PM10, PM2.5, and very fine particulate matter (PM1); (2) chemical characterization and source apportionment analysis of PM10 and PM2.5; and (3) intensive measurements in field campaigns to characterize the emission plumes of several point sources. Annual means of 37, 19, and 16 microg/m3 were obtained for the study period for PM10, PM2.5, and PM1, respectively. High PM episodes, characterized by a very fine grain size distribution, are frequently detected in Huelva mainly in the winter as the result of the impact of the industrial emission plumes on the city. Chemical analysis showed that PM at Huelva is characterized by high PO4(3-) and As levels, as expected from the industrial activities. Source apportionment analyses identified a crustal source (36% of PM10 and 31% of PM2.5); a traffic-related source (33% of PM10 and 29% of PM2.5), and a marine aerosol contribution (only in PM10, 4%). In addition, two industrial emission sources were identified in PM10 and PM2.5: (1) a petrochemical source, 13% in PM10 and 8% in PM2.5; and (2) a mixed metallurgical-phosphate source, which accounts for 11-12% of PM10 and PM2.5. In PM2.5 a secondary source has been also identified, which contributed to 17% of the mass. A complete characterization of industrial emission plumes during their impact on the ground allowed for the identification of tracer species for specific point sources, such as petrochemical, metallurgic, and fertilizer and phosphate production industries. 相似文献