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排序方式: 共有10000条查询结果,搜索用时 15 毫秒
141.
Jean-Marc Costa Alexandra Benachi Evelyne Gautier Jean-Marie Jouannic Pauline Ernault Yves Dumez 《黑龙江环境通报》2001,21(12):1070-1074
An Erratum has been published for this article in Prenatal Diagnosis 22(13) 2002, 1241. Fetal sex prediction can be achieved using PCR targeted at the SRY gene by analysing cell-free fetal DNA in maternal serum. Unfortunately, the results reported to date show a lack of sensitivity, especially during the first trimester of pregnancy. Therefore, determination of fetal sex by maternal serum analysis could not replace karyotype analysis following chorionic villus sampling. A new highly sensitive real-time PCR was developped to detect an SRY gene sequence in maternal serum. Analysis was performed on 121 pregnant women during the first trimester of pregnancy (mean gestational age: 11.8 weeks). Among them, 51 had at least one previous male-bearing pregnancy. Results were compared with fetal sex. SRY PCR analysis of maternal serum was in complete concordance with fetal sex. Among the 121 pregnant women, 61 were bearing a male fetus and 60 a female fetus. No false-negative results were observed. Furthermore, no false-positive results occurred, even though 27 women carrying a female fetus during the current pregnancy had at least one previous male-bearing pregnancy. This study demonstrates that a reliable, non-invasive sex determination can be achieved by PCR analysis of maternal serum during the first trimester of pregnancy. This non-invasive approach for fetal sex prediction should have great implications in the management of pregnant women who are carriers of an X-linked genetic disorder. Prenatal diagnosis might thus be performed for male fetuses only, avoiding invasive procedures and the risk of the loss of female fetuses. Copyright © 2001 John Wiley & Sons, Ltd. 相似文献
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JHERIME L. KELLERMANN MATTHEW D. JOHNSON AMY M. STERCHO STEVEN C. HACKETT 《Conservation biology》2008,22(5):1177-1185
Abstract: Coffee farms can support significant biodiversity, yet intensification of farming practices is degrading agricultural habitats and compromising ecosystem services such as biological pest control. The coffee berry borer (Hypothenemus hampei) is the world's primary coffee pest. Researchers have demonstrated that birds reduce insect abundance on coffee farms but have not documented avian control of the berry borer or quantified avian benefits to crop yield or farm income. We conducted a bird‐exclosure experiment on coffee farms in the Blue Mountains, Jamaica, to measure avian pest control of berry borers, identify potential predator species, associate predator abundance and borer reductions with vegetation complexity, and quantify resulting increases in coffee yield. Coffee plants excluded from foraging birds had significantly higher borer infestation, more borer broods, and greater berry damage than control plants. We identified 17 potential predator species (73% were wintering Neotropical migrants), and 3 primary species composed 67% of migrant detections. Average relative bird abundance and diversity and relative resident predator abundance increased with greater shade‐tree cover. Although migrant predators overall did not respond to vegetation complexity variables, the 3 primary species increased with proximity to noncoffee habitat patches. Lower infestation on control plants was correlated with higher total bird abundance, but not with predator abundance or vegetation complexity. Infestation of fruit was 1–14% lower on control plants, resulting in a greater quantity of saleable fruits that had a market value of US$44–$105/ha in 2005/2006. Landscape heterogeneity in this region may allow mobile predators to provide pest control broadly, despite localized farming intensities. These results provide the first evidence that birds control coffee berry borers and thus increase coffee yield and farm income, a potentially important conservation incentive for producers. 相似文献
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Rocío Mariano-Jelicich Florencia Botto Paulina Martinetto Oscar Iribarne Marco Favero 《Marine Biology》2008,155(4):443-450
The degree of individual or gender variation when exploiting food resources is an important aspect in the study of foraging
ecology within a population. Previous information on non-breeding skimmers obtained through conventional methodologies suggested
sex-related differences in prey species. In this study, stable isotope techniques were used to investigate the intraspecific
segregation in diet and foraging habits of the Black Skimmer (Rynchops niger intercedens) at Mar Chiquita Coastal Lagoon (37°40′S, 57°22′W), Argentina. These results were compared with contemporary data on the
trophic composition obtained by conventional methodologies. Blood samples were taken from birds captured with mist-nets during
their non-breeding season. The isotopic signatures of skimmers showed a diet mainly composed of marine prey with some degree
of estuarine fish intake. When comparing diet between sexes, males showed enrichment in 15N compared to females, while no differences were observed in 13C. The use of mixing models revealed differences in the relative composition of prey in the diet of male and female skimmers.
This study highlights stable isotope analysis as a valuable tool to test inter-individual differences and sexual segregation
in trophic ecology of Black Skimmers as compared to conventional methodologies. The results show a trophic segregation in
the Black Skimmer during the non-breeding season that can be explained by differences in prey species and larger prey sizes
of male skimmers. Our findings have significant implications for conservation since any environmental change occurring at
wintering areas might have profound effects on several avian life-history traits, and could be different for males and females
due to trophic segregation. 相似文献
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148.
Frank Eierdanz Joseph Alcamo Lilibeth Acosta-Michlik Dörthe Krömker Dennis Tänzler 《Regional Environmental Change》2008,8(4):197-205
This paper presents the technical aspects of a new methodology for assessing the susceptibility of society to drought. The
methodology consists of a combination of inference modelling and fuzzy logic applications. Four steps are followed: (1) model
input variables are selected—these variables reflect the main factors influencing susceptibility in a social group, population
or region, (2) fuzzification—the uncertainties of the input variables are made explicit by representing them as ‘fuzzy membership
functions’, (3) inference modelling—the input variables are used to construct a model made up of linguistic rules, and (4)
defuzzification—results from the model in linguistic form are translated into numerical form, also through the use of fuzzy
membership functions. The disadvantages and advantages of this methodology became apparent when it was applied to the assessment
of susceptibility from three disciplinary perspectives: Disadvantages include the difficulty in validating results and the
subjectivity involved with specifying fuzzy membership functions and the rules of the inference model. Advantages of the methodology
are its transparency, because all model assumptions have to be made explicit in the form of inference rules; its flexibility,
in that informal and expert knowledge can be incorporated through ‘fuzzy membership functions’ and through the rules in the
inference model; and its versatility, since numerical data can be converted to linguistic statements and vice versa through
the procedures of ‘fuzzification’ and ‘defuzzification’. 相似文献
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技术变化是应对气候变化问题的关键。对技术变化的模拟和分析能够识别技术变化的作用.在评估减排成本以及确定减排目标时起到重要作用。但是早期的气候政策模型通常将技术变化视为非经济的外生变量,因而无法对技术变化的起因和效果做深入分析。近年来的气候政策模型则开始逐渐利用内生方法模拟技术变化。在介绍内生技术变化的概念及与传统的外生技术变化的区别的基础上,重点介绍和评述研发投入和技术学习这两种主要的内生技术变化模拟方法的原理、特点以及如何在不同的气候政策模型中得到应用。但是现有建模方法无法表征技术变化过程中的动态性、不确定性等特征,因而传统的建模方法需要扩展到一个更为综合的框架并需要更坚实的实证研究基础上。基于该框架介绍内生技术变化模拟方法的未来可能拓展。 相似文献