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231.
Microbial diversity and abundance in bioaerosols of a coal mine were analyzed based on 454 pyrosequencing and real-time polymerase chain reaction(PCR). A total of 37,191 high quality sequences were obtained and could be classified into 531, 1730 and 448 operational taxonomic units respectively for archaea, bacteria and fungi at 97% sequence similarity. The Shannon diversity index for archaea, bacteria and fungi was respectively 4.71, 6.29 and 3.86, indicating a high diversity in coal mine bioaerosols. Crenarchaeota, Proteobacteria and Ascomycota were the dominant phyla for archaea, bacteria and fungi, respectively. The concentrations of total archaea, bacteria and fungi were 1.44 × 108, 1.02 × 108 and 9.60 × 104cells/m3, respectively.Methanotrophs observed in bioaerosols suggested possible methane oxidation in the coal mine. The identified potential pathogens to coal miners, such as Acinetobacter schindleri,Aeromonas cavernicola, Alternaria alternata, Aspergillus penicillioides, Cladosporium cladosporioides,and Penicillium brevicompactum were also observed. This was the first investigation of microbial diversity and abundance in coal mine bioaerosols. The investigation of microbial communities would be favorable in promoting the progress of methane control based on microbial technique and concern on coal miners’ health. 相似文献
232.
233.
Zhu N An P Krishnakumar B Zhao L Sun L Mizuochi M Inamori Y 《Journal of environmental management》2007,85(4):936-943
The emission of methane from two constructed wetlands [a free water surface flow system (FWS) and a subsurface flow system (SF)], constructed for the treatment of waste water, was evaluated at different sites inhabited by reeds (Phragmites communis), to test the effects of plant harvest. High methane emission was recorded immediately after harvesting in both wetlands. Several days after harvesting, the emission decreased in the FWS but remained high in the SF. The variation was significantly influenced by temperature, with lower emission and higher dissolved CH(4) in water occurring at lower temperatures. Both the emission and concentration of dissolved CH(4) were also influenced significantly by water quality, wetland design, level of stalk butt left above the water level, etc. The methane flux was explained on the basis of rizhospheric methanogenic and methanotrophic microbial populations. FISH analysis indicated the presence of Type A and Type B methanotrophs in both wetlands, and the methane flux was directly influenced by the quantitative variation in methanogenic and methanotrophic bacteria in both wetlands. 相似文献
234.
Environmental contamination of heavy metals from zinc smelting areas in Hezhang County, western Guizhou, China 总被引:14,自引:0,他引:14
Total heavy metal (Cd, Cr, Cu, Pb and Zn) concentrations were evaluated in smelting waste, soil, crop and moss samples collected from the Hezhang artisanal zinc smelting areas, Guizhou, China. Soil samples from the cornfield near the smelting sites contained extremely high Cd (5.8-74 mg kg(-1)), Pb (60-14,000 mg kg(-1)) and Zn (260-16,000 mg kg(-1)) concentrations. Elevated heavy metal concentrations were also found in corn plants and total Pb (0.80-1.5 mg kg(-1)) and Cd (0.05-0.76 mg kg(-1)) concentrations in corn grain have totally or partially exceeded the national guidance limits for foodstuff. Thus, the soil-to-crop transfer of heavy metals might pose a potential health risk to the local residents. Similar to the high heavy metal levels in soil and corn, Cd, Cr, Cu, Pb and Zn concentrations in moss samples collected from the smelting sites ranged from 10 to 110, 10 to 55, 26 to 51, 400 to 1200 and 330 to 1100 mg kg(-1), respectively, exhibiting a local spatial pattern of metals deposition from the atmosphere. Based on examination of Zn/Cd and Pb/Cd ratios of the analyzed samples, we have distinguished between the flue gas dust derived and smelting waste derived metals in different environmental compartments. 相似文献
235.
通过资料调研、现场调查与检测,将获取的资料与法律、法规和标准要求相比较,对某炼化企业催化汽油吸附脱硫装置进行了职业病危害控制效果评价。 相似文献
236.
Soil ecotoxicity assessment using cadmium sensitive plants 总被引:15,自引:0,他引:15
An YJ 《Environmental pollution (Barking, Essex : 1987)》2004,127(1):21-26
Four crop plant species (sweet corn, Zea may; wheat, Triticum aestivum; cucumber, Cucumis sativus; and sorghum, Sorghum bicolor) were tested to assess an ecotoxicity in cadmium-amended soils. The measurement endpoints used were seed germination and seedling growth (shoot and root). The presence of cadmium decreased the seedling growth. The medium effective concentration values (EC50) for shoot or root growth were calculated by the Trimmed Spearman-Karber method. Due to the greater accumulation of Cd to the roots, root growth was a more sensitive endpoint than shoot growth. Bioavailability and transport of Cd within plant were related to concentration and species. The ratio of bioaccumulation factor (BAF) in the shoots to the roots indicated high immobilization of Cd in the roots. Seed germination was insensitive to Cd toxicity, and is not recommended for a suitable assay. Among the test plants and test endpoints, root growth of sorghum and cucumber appears to be a good protocol to assess ecotoxicity of soils contaminated by Cd. 相似文献
237.
自导式解说系统设计要素研究--以邓小平故居景区为例 总被引:7,自引:0,他引:7
以邓小平故居自导式解说系统的实际设计、制作为例,重点研究了自导式解说系统在设计中应当把握的四大要素,并对这四大设计要素在设计过程中的相互关系进行了理论研究和实例分析,指出这四大要素是旅游区设计出高水平、高质量的自导式解说系统的关键核心,从而使自导式解说系统成为提升旅游区整体形象的重要组成部分. 相似文献
238.
239.
示波极谱测定空气中甲醛含量的方法及其应用 总被引:6,自引:0,他引:6
本文研究了用示波极谱测定室内空气中甲醛含量的方法,其结果为:本法测定甲醛的检测下限为34μg/L,加标回收率为875~1100%,变异系数为425~863%,该法灵敏度高,甲醛含量与极谱峰高的线性关系好,是一种可靠的测定室内空气中甲醛含量的方法。同时还研究了缓冲液的pH值及吸收液放置时间对甲醛测定结果的影响,并将该法应用于评价空气净化器去除室内空气中甲醛的效果。 相似文献
240.
An M. Michelsen 《Journal of the American Water Resources Association》1994,30(6):971-982
ABSTRACT: Voluntary water transfers through markets have been advocated by many diverse groups as a means to reallocate scarce water supplies in the semi-arid western U.S. Although transfers of water rights have occurred almost since the creation of prior appropriation laws over a century ago, functioning water markets have been very slow to develop and are few in number. The structure, composition, administration and transactions of one of the most well established water markets, shares in the Bureau of Reclamation, Colorado-Big Thompson project, are examined to better understand the institutional and transfer conditions that sustain an active water market. Results from a detailed study of C-BT project records reveal that between 1970 and 1993 there were 2,698 transactions through which over one-third of the project water changed ownership or type of use. Further analysis shows that the transactions involved many individual sellers and categories of buyers with different uses, including agricultural buyers. The transfer activity and efficiency of the C-BT market has lead some to suggest that it be used as a model for other markets. However, because this market has fewer institutional restrictions, a well developed infrastructure and unique market conditions, it will be difficult to transfer this model to other areas without accompanying modifications in water right administration and institutions. 相似文献