Sesquiterpene Hydrocarbons: Kairomones with a releaser effect in the sexual communication of the white-spotted longicorn beetle, <Emphasis Type="Italic">Anoplophora malasiaca</Emphasis> (Thomson) (Coleoptera: Cerambycidae)

Summary.  We conducted a series of experiments with the white-spotted longicorn beetle Anoplophora malasiaca (Thomson), and its host plant, Citrus unshiu, to examine the origin of the sesquiterpene hydrocarbons (SHCs; including β-elemene, β-caryophyllene, α-humulene, α-farnesene, and several unidentified compounds) that are contained in the elytra of the beetles and act as an attractant. In the laboratory, mechanically wounded citrus branches, as well as those fed upon by A. malasiaca, attracted males more frequently than intact branches. Solid phase microextraction (SPME) and subsequent analyses by gas chromatography (GC) analyses detected measurable SHCs from the air around both mechanically wounded and beetle-infested branches, as well as trace amounts from intact branches. The SHCs were also detected for a certain time from beetles that had fed on the citrus branches, but the amounts decreased rapidly after they were removed from the host. This decrease generally corresponded with a reduction of attractiveness of the beetles in a behavioural assay. Isolated females acquired the SHCs after exposure to, but not upon contact with, other females that had fed on C. unshiu branches. We hypothesize that the citrus SHCs are adsorbed in, retained on, and released from the wax layer of the beetle elytra. Since these compounds are released from branches when beetles feed, they may indirectly signal the presence of beetles to others in the field. The high response rate to SHCs by males is likely representing mate searching behavior. The SHCs act as kairomones with a releaser effect in the communication system of A. malasiaca. Hiroe Yasui, Toshiharu Akino, Midori Fukaya: These authors contributed equally to this article.     

Cannibalize or care? The role of perceived paternity in the sand goby, <Emphasis Type="Italic">Pomatoschistus minutus</Emphasis>

Parental care is a costly part of reproduction. Hence, natural selection should favor males which avoid caring for unrelated young. However, the decision to abandon or reduce care requires cues which are evaluated to give information on potential reproductive value of the offspring. The prediction that male sand gobies, Pomatoschistus minutus, care for foreign eggs as long as they were spawned in their own nest and at least some of such cues are fulfilled was tested. Egg-guarding males that had recently taken part in a spawning event were given a clutch of eggs that was sired either by themselves or another male, in either their own or another male’s aquarium. Males that had not taken part in a spawning event were used as controls and were given eggs sired by another male. We measured the amount of filial cannibalism and nest building. Control group males did not care for eggs and ate them all before rebuilding the nest. In the other treatments, there were no significant effects of paternity, though males moved to another male’s aquarium increased their clutch area threshold and completely consumed larger clutches than males that were not moved. There was no intermediate response in any treatment in the form of increased partial filial cannibalism or less well-constructed nests. Our results suggest that egg-guarding males cannot distinguish between eggs sired by themselves and those sired by other males but are able to react to cues indicating paternity state. Males do not adopt eggs to attract females in P. minutus.     

Experimental study of recirculating flows generated by lateral shock waves in very large channels

Due to the lack of data on hydraulic-jump dynamics in very large channels, the present paper describes the main characteristics of the velocity field and turbulence in a large rectangular channel with a width of 4 m. Although a hydraulic jump is always treated as a wave that is transversal to the channel wall, in the case of this study it has a trapezoidal front shape, first starting from a point at the sidewalls and then developing downstream in an oblique manner, finally giving rise to a trapezoidal shape. The oblique wave front may be regarded as a lateral shockwave that arises from a perturbation at a certain point of the lateral wall and travels obliquely toward the centreline of the channel. The experimental work was carried out at the Coastal Engineering Laboratory of the Water Engineering and Chemistry Department of the Technical University of Bari (Italy). In addition to the hydraulic jump formation, a large recirculating flow zone starts to develop from the separating point of the lateral shock wave and a separate boundary layer occurs. Intensive measurements of the streamwise and spanwise flow velocity components along one-half width of the channel were taken using a bidimensional Acoustic Doppler Velocimeter (ADV). The water surface elevation was obtained by means of an ultrasonic profiler. Velocity vectors, transversal velocity profiles, turbulence intensities and Reynolds shear stresses were all investigated. The experimental results of the separated boundary layer were compared with numerical predictions and related work presented in literature and showed good agreement. The transversal velocity profiles indicated the presence of adverse pressure gradient zones and the law of the wall appears to govern the region around the separated boundary layer.     

Lipid and protein utilisation during early development of yellowtail kingfish (<Emphasis Type="Italic">Seriola lalandi</Emphasis>)

The pelagic yellowtail kingfish Seriola lalandi has become a target species for aquaculture in Asia and Australasia. Australasian production is reliant on larviculture from eggs of captive brood stock; however, knowledge regarding the nutritional requirements of larvae of this species is still scarce, particularly in relation to lipids. As a first step in establishing these requirements, eggs and larvae from captive S. lalandi brood stock were examined for differences in total protein, total lipid and lipid classes between individual spawning events, over the spawning season, and during larval development from fertilisation to 15 days post hatch. Results indicate that total protein egg⁻¹ varied significantly between individual spawning events within a season, but neither total lipid nor total protein egg⁻¹ varied significantly across the spawning season. Brood stock egg lipids were made up of approximately 60% phospholipid, 25% wax and/or sterol esters (WE), 15% triacylglycerol (TAG), and small amounts of sterols and free fatty acids. During the early larval period, both WE and TAG were utilised concurrently for energy. The larvae experienced very high mortality around 5–7 days post hatch, which coincided with very low levels of all neutral lipid classes. Although many other factors may also influence larval mortality, these results indicate that lipid provisioning may be an important factor in larval survival during the critical period around first-feeding in this species. Examination of ratios of TAG:ST, often used as a condition index in fish larvae, suggested that some of the larvae were suffering from starvation. However, as egg-derived WE appears to provide a significant source of energy during the early larval period in S. lalandi, it is suggested that WE should be included in any index of larval nutritional state.     

Rats on the run: removal of alien terrestrial predators affects bush rat behaviour

Predators can strongly influence the microhabitat use and foraging behaviour of prey. In a large-scale replicated field experiment in East Gippsland, Australia, we tested the effects of reduced alien red fox (Vulpes vulpes) and alien wild dog (Canis lupus familiaris) abundance (treatment) on native bush rat (Rattus fuscipes) behaviour. Bush rats are exposed to two main guilds of predators, namely mammalian carnivores and birds of prey. Tracking rat movements using the spool-and-line technique revealed that, in treatment sites, rats used ground cover, which provides shelter from predators, less often than at unmanipulated fox and wild dog abundance (non-treatment sites). In treatment sites, rats more frequently moved on logs where they would have been exposed to hunting foxes and dogs than in non-treatment sites. Furthermore, in treatments, rats showed a preference for understorey but not in non-treatments. Hence, bush rats adapted their behaviour to removal of alien terrestrial predators. Giving-up densities (GUDs) indicated no treatment effects on the marginal feeding rate of bush rats. Interestingly, GUDs were higher in open patches than in sheltered patches, suggesting higher perceived predation risk of bush rats during foraging at low versus high cover. The lack of treatment effects on GUDs but the clear response of bush rats to cover may be explained by the impact of predators other than foxes and wild dogs.     

Transient ontogenetic expression of hermaphroditic gonad morphology within the Gobiosoma group of the Neotropical seven-spined gobies (Teleostei: Gobiidae)

The Neotropical seven-spined gobies (tribe Gobiosomatini), including the Gobiosoma and Microgobius groups, constitute a speciose, monophyletic gobiid taxon. In particular, member species of the Gobiosoma group exhibit a combination of behavioral diversification and microhabitat specialization that may have played a major role in their collective rapid evolution and high rate of speciation. Functional hermaphroditism, which is common among gobiids, can promote exploitation of patchily distributed micro-niches by countering potential reductions in reproductive opportunities. However, the possible role of functional hermaphroditism in the exploitation of microhabitats and rapid speciation in the Gobiosoma group is unknown. One member species, Tigrigobius multifasciatus, is a functional hermaphrodite. Two other Gobiosoma group species are functionally gonochoric (i.e., constant-sexed), but exhibit transient hermaphroditic ovarian structure among immatures. In this study, ovarian morphology among immature and adult females of several Gobiosoma group species was examined to see if hermaphroditic gonadal features are present. Although no evidence of functional hermaphroditism in the form of precursive accessory gonadal structures (pAGS) associated with the adult ovary was found among newly examined species, all species exhibited the transient expression of hermaphroditic gonadal features associated with the immature ovary. In contrast, among six species of non-Gobiosomatini genera having no record of hermaphroditism, none exhibited similar transient hermaphroditic features associated with the immature ovary. These findings suggest that hermaphroditism may have been an ancestral trait which has been secondarily lost within some Gobiosoma group species. This study also shows that ontogenetic and morphogenic processes within the gobiid reproductive system may provide new insights into the evolution of life history traits and significantly further our understanding of the extraordinary evolutionary success of this group.     

Prior encounters with the opposite sex affect male and female mating behavior in a wolf spider (Araneae,Lycosidae)

Mate availability can vary widely in nature depending upon population density and sex ratio and can affect the ability of individuals to be selective in mate choice. We tested the effects of prior encounters with the opposite sex (i.e., exposure to the opposite sex either with or without mating) on subsequent mating behavior in two experiments that manipulated mate availability for both males and females in the wolf spider, Hogna helluo. The probability of mating in the experimental trial depended upon whether the prior encounter involved mating or not, and males and females responded in opposite directions. Exposure without mating resulted in a higher subsequent frequency of mating for females and a lower subsequent frequency of mating for males, while prior mating experience resulted in a lower frequency of female remating and a higher frequency of male remating. Prior exposure without mating did not affect female aggression. However, mated females engaged in precopulatory cannibalism more frequently than virgins. Mated males escaped postcopulatory cannibalism more frequently than virgins. Our results show that males respond to exposure without mating in the expected manner. However, prior mating (1 week earlier) had unexpected effects on males, which may be due to mated males being of higher quality. There were little or no effects of the size of the prior exposure individual or mate on subsequent mating behaviors. Further research is needed to determine why different species use different degrees of prior information in mate choice.     

Accounting for rate instability and spatial patterns in the boundary analysis of cancer mortality maps

Boundary analysis of cancer maps may highlight areas where causative exposures change through geographic space, the presence of local populations with distinct cancer incidences, or the impact of different cancer control methods. Too often, such analysis ignores the spatial pattern of incidence or mortality rates and overlooks the fact that rates computed from sparsely populated geographic entities can be very unreliable. This paper proposes a new methodology that accounts for the uncertainty and spatial correlation of rate data in the detection of significant edges between adjacent entities or polygons. Poisson kriging is first used to estimate the risk value and the associated standard error within each polygon, accounting for the population size and the risk semivariogram computed from raw rates. The boundary statistic is then defined as half the absolute difference between kriged risks. Its reference distribution, under the null hypothesis of no boundary, is derived through the generation of multiple realizations of the spatial distribution of cancer risk values. This paper presents three types of neutral models generated using methods of increasing complexity: the common random shuffle of estimated risk values, a spatial re-ordering of these risks, or p-field simulation that accounts for the population size within each polygon. The approach is illustrated using age-adjusted pancreatic cancer mortality rates for white females in 295 US counties of the Northeast (1970–1994). Simulation studies demonstrate that Poisson kriging yields more accurate estimates of the cancer risk and how its value changes between polygons (i.e., boundary statistic), relatively to the use of raw rates or local empirical Bayes smoother. When used in conjunction with spatial neutral models generated by p-field simulation, the boundary analysis based on Poisson kriging estimates minimizes the proportion of type I errors (i.e., edges wrongly declared significant) while the frequency of these errors is predicted well by the p-value of the statistical test.

Phylogeography of the Southwestern Atlantic menhaden genus <Emphasis Type="Italic">Brevoortia</Emphasis> (Clupeidae,Alosinae)

Among pelagic fish, the Southwestern Atlantic menhaden genus Brevoortia (Clupeidae, Alosinae) constitutes an important species model to investigate the patterns of genetic differentiation. It is abundant in the Río de la Plata estuary and in the Atlantic coastal lagoons system from Uruguay and Southern Brazil. To access in the taxa discrimination and population structure in Brevoortia we perform a phylogeographic approach based on mitochondrial cytochrome b (cyt-b) sequences including 240 individuals from 16 collecting sites. Among the 720 bp cyt-b sequenced, 199 correspond to variables and 88 to phylogenetically informative sites. High values of haplotype diversity (h = 1.000) and nucleotide diversity (π = 0.061), as well as an average of 0.084 polymorphic segregating sites and 46 different haplotypes were found. Maximum likelihood analysis based on the GTR + I + G model and Bayesian inference strongly support the idea that B. aurea is the only species of the genus inhabiting the Southwestern Atlantic region. Our analyses revealed a complex population pattern characterized by the existence of long-term highly structured genetic assemblages of mixed stocks. Each monophyletic entity included individuals from different collecting sites, different age groups and collected in different years. Our data also suggest that the recruitment of unrelated mtDNA haplotypes carried out by individuals within schools could be occurring in the same nursery areas revealing the existence of many different maternal lineages. A scenario where different simultaneously and successively mixed mtDNA lineages remain historically connected through basal haplotypes among different clades could explains more accurately the complex and ordered metapopulation dynamic found in this pelagic fish.     

Temporal patterns of larval settlement and survivorship of two broadcast-spawning acroporid corals

Acroporid corals are the main reef-building corals that provide three-dimensional habitats for other reef organisms, but are decreasing on many reefs worldwide due to natural and anthropogenic disturbances. In this study, temporal patterns of larval settlement and survivorship of two broadcast-spawning acroporid coral species, Acropora muricata and A. valida, were examined through laboratory rearing experiments to better understand the potential for larval dispersal of this important coral group. Many larvae were attached (but not metamorphosed) to settlement tiles on the first examination 3–4 days after spawning (AS). The first permanent larval settlement (i.e. metamorphosed and permanently settled juvenile polyps) occurred at 5–6 days AS, and most larval settlement (85–97% of total) occurred within 9–10 days AS. Larval survivorship decreased substantially to around 50% by the first week of the experiment and to approximately 10% by the second to third week. The rates of larval attachment, settlement, and the initial drop in survivorship of larvae suggest that effective dispersal of some acroporid species may largely be completed within the first few weeks AS.