Secondary imbibition in NAPL-invaded mixed-wet sediments

A previously developed pore network model is used here to study the spontaneous and forced secondary imbibition of a NAPL-invaded sediment, as in the displacement of NAPL by waterflooding a mixed-wet soil. We use a 3D disordered pore network with a realistic representation of pore geometry and connectivity, and a quasi-static displacement model that fully describes the pore-scale physics. After primary drainage (NAPL displacing water) up to a maximum capillary pressure, we simulate secondary imbibition (water displacing NAPL). We conduct a parametric study of imbibition by varying systematically the controlling parameters: the advancing contact angles, the fraction of NAPL-wet pores, the interfacial tension, and the initial water saturation. Once the secondary imbibition is completed, the controlling displacement mechanisms, capillary pressures, relative permeabilities, and trapped NAPL saturations are reported. It is assumed that NAPL migrates into an initially strongly water-wet sediment, i.e., the receding contact angles are very small. However, depending on the surface mineralogy and chemical compositions of the immiscible fluid phases, the wettability of pore interiors is altered while the neighborhoods of pore corners remain strongly water-wet-resulting in a mixed-wet sediment. Here, we compare three different levels of wettability alteration: water-wet (advancing contact angles (20 degrees to 55 degrees), intermediate-wet (55 degrees to 120 degrees), and NAPL-wet (120 degrees to 155 degrees). The range of advancing contact angles and the fraction of NAPL-wet pores have dramatic effects on the NAPL-water capillary pressures and relative permeabilities. The spatially inhomogeneous interfacial tension has a minor impact on the trapped NAPL saturation and relative permeability to NAPL, and a slight effect on the relative permeability to water. The initial water saturation has a slight effect on the two-phase flow characteristics of water-wet sediments; however, with more NAPL-wet pores in the sediment, it starts to have a profound effect on the water and NAPL relative permeabilities.     

Greenhouse gas emission from small clinics solid waste management scenarios in an urban area of an underdeveloping country: A life cycle perspective

This study aimed to investigate the effects on the environment of small clinics solid waste management by applying a life cycle analysis approach. Samples were collected from 371 private clinics situated in densely populated areas of Hyderabad, Pakistan. The solid waste from surveyed clinics was categorically quantified on daily basis for 30 consecutive days. The functional unit for waste was defined as 1 tonne. System limitations were defined as landfilling, incineration, composting, material recovery, and transportation of solid waste. The treatment and disposal methods were assessed according to their greenhouse gas emission rate. For the evaluation, three different scenarios were designed. The second scenario resulted in the highest emission value of 1491.78 kg CO₂ eq/tonne of solid waste due to mixed waste incineration, whereas the first scenario could not offer any saving because of uncovered landfilling and 67.5% higher transport fuel consumption than the proposed network. The proposed third scenario was found to be a better solution for urban clinics solid waste management, as it resulted in savings of 951.38 kg CO₂ eq/tonne of solid waste. This integrated design is practicable by resource-constrained economy. This system consists of composting, material recovery, and incineration of hazardous waste. The proposed system also includes a feasible transportation method for urban area collection networks. The findings of the present study can play a vital role in documenting evidence and for policymakers to plan the solid waste management of clinics, as previously no studies have been conducted on this particular case.

Implications: This study aims to highlight the impact of small clinics solid waste management scenarios on the environment in a developing country’s urban area. Life cycle analysis is used for comparison of greenhouse gase emission from different scenarios, including the purposed integrated method. Small clinics play a very important role in health care, and their waste management is a very serious issue; however, there are no previous studies on this particular case to the best knowledge of the authors. This study can be considered as forerunner effort to quantify the environmental footprint of small clinics solid waste in urban areas of a developing country.     

Modeling Change-Pattern-Value Dynamics on Land Use: An Integrated GIS and Artificial Neural Networks Approach

The use of spatial methods to detect and characterize changes in land use has been attracting increasing attention from researchers. The objectives of this article were to formulate the dynamics of land use on the temporal and spatial dimensions from the perspectives of the Change-Pattern-Value (CPV) and driving mechanism, based on multitemporal remote sensing data and socioeconomic data. The Artificial Neural Networks were used to identify the factors driving changes in land use. The Pearl River Delta Region of southeast China, which was experiencing rapid economic growth and widespread land conversion, has been selected as the study region. The results show that from 1985 to 2000 in the study region (1) the most prominent characteristics of change in land use were the expansion of the urban land at the expense of farmland, forests, and grasslands, (2) the land-use pattern was being optimized during this period, (3) in an analysis of value, built-up land can yield a return of more than 30 times that of farmland, water area, and forests lands, and (4) rapid economic development, growth in population, and the development of an infrastructure were major driving factors behind ecological land loss and the nonecological land expansion.     

A SIMULATION OF SMOOTH-VARIABLE INTENSITY RAINFALL PATTERNS1

ABSTRACT: Continuous rainfall patterns are currently simulated by approximating them by stair-stepped (piece-wise) patterns. The effects of this approximation on infiltration and runoff processes are not well known. A new technique for simulating smooth-variable intensity rainfall patterns is presented. This technique is based on the fundamental principles of a moving water head in a container. The proposed technique is general and capable of simulating any rainfall pattern. However, as the rainfall pattern gets more complicated, the equipment required for simulation becomes more involved. The proposed technique has been tested experimentally. A close agreement was found between the theoretical and experimental simulations. It is concluded that the proposed technique might be very useful in studying the infiltration and runoff processes under variable intensity rainfall, especially for simple convex patterns.     

The rhetoric of participation re-examined: The state,NGOs and water users at Varanasi,Uttar Pradesh,India

Summary People's participation is usually regarded as a sine qua non for the success and sustainability of development projects. Yet in practice, it raises a number of questions. Who are the people? Why is their participation sought, and how or at what level, is such participation desired? This paper seeks to examine the rhetoric of participation in the implementation of the Ganga Action Plan (GAP) at Varanasi, in the north-eastern State of Uttar Pradesh, India. Launched in 1985, the GAP is the first major attempt to systematically control and monitor the pollution of a significant river in the country. In addition, it claimed to be a people's programme because of the powerful and deep-seated cultural and religious meaning associated with the Ganga. Varanasi, however, is indicative of its failure to deliver this promise — the GAP is only acceptable to authority because it does not challenge the existing institutional order, and its participatory content is symbolic rather than substantive. Non-govemental organisations, traditionally viewed as intermediary actors between the micro and macro levels, work within the socio-political framework of the city. In the process, water-user groups such as the washermen who derive an economic livelihood from washing clothes in the Ganga, are literally excluded from the definition and process of participation.She obtained her PhD, the basis of the current research, at the University of Cambridge, UK.     

Correlation between oxygen utilization and electron transport activity in marine phytoplankton

Measurement of a respiratory control ratio is deemed very useful in determination of energy balance in respiration. We have defined and computed a control ratio as the ratio of the rate of oxygen consumption in vivo to the calculated maximum rate of oxygen consumption in vitro as measured by enzymatic electron transport rates. In order for a control ratio to serve a useful purpose in calculating in situ oxygen consumption in a water parcel, the ratio must be fairly constant. Our results indicated that this is precisely so in healthy cultures of the 9 species of phytoplankton studied. The data presented in this communication further point towards the potential for making reasonable estimates of phytoplankton oxygen consumption in an ocean water parcel by directly utilizing in vitro electron transport activity measurements.     

Species differences in accumulation of nitrogen pools in phytoplankton

The changes in the intracellular concentrations of nitrate, ammonium, free amino acids, protein, DNA, RNA and total nitrogen were measured in batch cultures of seven species of marine phytoplankton as they progressed from being nitrogen sufficient to being nitrogen starved. After several days of nitrogen starvation, either nitrate or ammonium was added to the cultures, and the measurements were continued for 10 to 36 h. By this means it was possible to assess the long-term and short-term changes in cellular nitrogen compounds and how they relate to phytoplankton nitrogen uptake and growth. Considerable species differences were observed in the amounts and kinds of nitrogen compounds which were stored and the degree to which the utilization of these compounds could support growth if the external nitrogen supply is low or variable. Despite the species variation, the results suggest that the concentrations or ratios of a number of intracellular nitrogen compounds can be used to assess the nitrogen deficiency and/or growth rate of natural phytoplankton populations.Contribution No. 1373 from the School of Oceanography, University of Washington and Contribution No. 83006 from the Bigelow Laboratory for Ocean Sciences     

Prenatal diagnosis of familial ring 21 chromosome

Ring chromosome 21 is a rare chromosome anomaly often associated with mental retardation and dysmorphic features. Less commonly, the ring chromosome can be familial and associated with a normal phenotype. Phenotypically normal female carriers, however, are at increased risk of having children with Down syndrome, mosaic monosomy 21, and variable duplication or deletion of chromosome 21. Because of the relative mitotic and meiotic instability of ring chromosomes, abnormal cytogenetic findings encountered during prenatal diagnosis may not reflect the true genetic status of the fetus. This is a report of a phenotypically normal female carrier of a familial ring 21 chromosome. Prenatal diagnosis on her twin pregnancy revealed a mosaic 46,XX,r(21)(p13;q22) (77 per cent)/45,XX, – 21 in one fetus and a normal male karyotype in the second. The pregnancy was carried to term. Both infants are completely normal, with a non-mosaic ring 21 karyotype from the lymphocytes of one twin. The diagnostic uncertainty and problematic genetic counselling related to fetal cytogenetic abnormalities are the subjects of this report.     

Advantages and disadvantages of the use of irradiation for food preservation

Food irradiation is a physical method of processing food (e.g. freezing, canning). It has been thoroughly researched over the last four decades and is recognized as a safe and wholesome method. It has the potential both of disinfesting dried food to reduce storage losses and disinfesting fruits and vegetables to meet quarantine requirements for export trade. Low doses of irradiation inhibit spoilage losses due to sprouting of root and tuber crops. Food- borne diseases due to contamination by pathogenic microorganisms and parasites of meat, poultry, fish, fishery products and spices are on the increase. Irradiation of these solid foods can decontaminate them of pathogenic organisms and thus provide safe food to the consumer. Irradiation can successfully replace the fumigation treatment of cocoa beans and coffee beans and disinfest dried fish, dates, dried fruits, etc. One of the most important advantages of food irradiation processing is that it is a coldprocess which does not significantly alter physico-chemical characters of the treated product. It can be applied to food after its final packaging. Similar to other physical processes of food processing, (e.g. canning, freezing), irradiation is a capital intensive process. Thus, adequate product volume must be made available in order to maximize the use of the facility and minimize the unit cost of treatment. Lack of harmonization of regulations among the countries which have approved irradiated foods hampers the introduction of this technique for international trade. Action at the international level has to be taken in order to remedy this situation. One of the important limitations of food irradiation processing is its slow acceptance by consumers, due inter alia to a perceived association with radioactivity. The food industry tends to be reluctant to use the technology in view of uncertainties regarding consumer acceptance of treated foods. Several market testing and consumer acceptance studies have been carried out on food irradiation in recent years. These studies showed that, if the safety and the benefits of food irradiation were properly explained, the consumers were willing to accept irradiated foods. Considering its potential role in the reduction of post-harvest losses, providing safe supply of food and overcoming quarantine barriers, food irradiation has received wider government approvals during the last decade. There is also a trend towards increased commercialization of irradiated food. Currently, there are 47 irradiation facilities in some 23 countries being used for treating foods for commercial purposes.     

Purification and biochemical characterization of recombinant alcohol dehydrogenase from the psychrophilic bacterium <Emphasis Type="Italic">Pseudomonas</Emphasis><Emphasis Type="Italic">frederiksbergensis</Emphasis>

A gram-negative psychrophilic bacterium, with potential for biodegradation of long-chain n-alkanes was isolated from ice samples collected in Spitzbergen, Denmark. On the basis of results of biochemical and morphological tests and sequence analysis of 16S rRNA, the strain was identified as Pseudomonas frederiksbergensis. In this work, a short-chain NAD⁺-dependent alcohol dehydrogenase (alcDH) (Accession number: AAR13804) from the P. frederiksbergensis was cloned and transformed in E. coli BL21 (3DE) competent cells. The alcDH activity was highest in the crude extract of cells induced with 1.0 mM IPTG. The recombinant alcDH enzyme was purified to 93.4% homogeneity using three consecutive purification steps including ammonium sulphate, Q-Sepharose Fast Flow column and gel filtration chromatography employing Superdex 200 10/30 HR column. Enzyme enrichment and yield levels of 31.4 folds and 25.5%, respectively, were achieved. While the subunit molecular mass of the enzyme was determined on SDS-PAGE to be ~38 kDa, the aggregated native form of the enzyme had a molecular mass of ~238 kDa by gel filtration analysis. Reaction conditions optima for the recombinant alcDH were determined with propan-1-ol as the substrate. While the optimum pH was 9, the optimum temperature was 35 °C. The alcDH enzyme exhibited moderate thermal stability with half-lives of 150 min at 55 °C, 27 min at 65 °C and 8 min at 75 °C. Results for kinetic parameters indicated that the apparent K _m value for alcDH with propan-1-ol as the substrate was found to be 1.42 mM and the V _max value was 0.63 mmol mg⁻¹ min⁻¹. Experimental evidence revealed that the recombinant alcDH exhibited a wide range of substrate specificity, with higher levels of specific activity for aliphatic alcohols as compared to secondary alcohols. Taken together, the present study highlights the potential of alcDH as a member of cold-adapted enzymes in several key biotechnological applications including environmental bioremediation and biotransformations. It is envisaged that, with the ongoing screening of microorganisms and metagenomes, directed evolution approaches and the subsequent overexpression of recombinant proteins, more enzymes will be found that are suitable for bioremediation purposes.