Retinal cryptochrome in a migratory passerine bird: a possible transducer for the avian magnetic compass

The currently discussed model of magnetoreception in birds proposes that the direction of the magnetic field is perceived by radical-pair processes in specialized photoreceptors, with cryptochromes suggested as potential candidate molecules mediating magnetic compass information. Behavioral studies have shown that magnetic compass orientation takes place in the eye and requires light from the blue-green part of the spectrum. Cryptochromes are known to absorb in the same spectral range. Because of this we searched for cryptochrome (CRY) in the retina of European robins, Erithacus rubecula, passerine birds that migrate at night. Here, we report three individually expressed cryptochromes, eCRY1a, eCRY1b, and eCRY2. While eCRY1a and eCRY2 are similar to the cryptochromes found in the retina of the domestic chicken, eCRY1b has a unique carboxy (C)-terminal. In light of the radical-pair model, our findings support a potential role of cryptochromes as transducers for the perception of magnetic compass information in birds.     

Variation among populations of the sailfin molly in the rate of concurrent multiple paternity and its implications for mating-system evolution

We examined patterns of concurrent multiple mating in a live-bearing poeciliid fish, the sailfin molly (Poecilia latipinna). We tested whether the probability of multiple paternity was related to female body size or fertility and whether the rate of multiple paternity varied among four populations that differed in their distributions of female body size and fertility. We analyzed data on mother and offspring genotypes for three polymorphic allozymes by three techniques, including a maximum-likelihood estimator that accounts for sampling error in both parental and offspring allele frequencies. The estimated rate of multiple paternity varied between 0.09 and 0.85, and the rate in one population varied seasonally between 0.33 (spring) and 0.85 (autumn). The variation in these rates was not associated with variation in body-size distributions among populations but was closely associated with variation in size-specific fertility: populations with greater variation in female fertility had higher multiple-paternity rates. Within two populations, logistic regression revealed that individual females of larger body size and greater size-specific fertility were more likely to carry multiply sired broods. This result is consistent with observations made in one of the populations 5 years earlier. In general, the results strongly suggest that the mating system varies markedly among conspecific populations of sailfin mollies and that larger, more fertile females are the objects of intermale competition. Received: 6 May 1996 / Accepted in revised form: 5 December 1996     

Female behaviour mediates male courtship under predation risk in the guppy (Poecilia reticulata)

Previous work has shown that under elevated predation risk, male guppies (Poecilia reticulata) switch from courtship to less conspicuous coercive mating attempts. This behavioural transition is traditionally interpreted as a 'risk-sensitive' response that makes males less conspicuous to predators. However, predation risk leads to behavioural changes (such as schooling and predator inspection) in females that may result in coercive mating attempts being more profitable in high-risk situations. Here, we tested the hypothesis that the switch to coercive mating by male guppies in high-risk situations is mediated by adjustments in female behaviour, rather than directly by the predator. We used replicate models resembling a known guppy predator to simulate predation risk in wild-caught guppies from a high-predation population in Trinidad. Our results revealed that males performed proportionately more coercive mating attempts when presented with a female that had been exposed previously to a model predator compared to when males were paired with non-exposed females. Total mating activity (combined rates of courtship and forced mating attempts) did not differ significantly among the two treatment groups, indicating that overall mating activity is unaffected by predation risk. Importantly, when we subsequently presented both sexes concurrently with a predator model, total mating activity and the proportion of forced mating attempts remained unchanged in the high-risk treatment. Taken together, these results indicate that the transition from courtship to forced mating attempts under elevated predation risk is mediated by changes in female behaviour, which we suggest may favour the use of coercive mating under high predation risk.     

Aluminium levels in the blood plasma of persons working in the aluminium industry

Due to the exposure to alumina in the air, workers in the primary aluminium industry are expected to exhibit a higher aluminium exposure than the general population. The additional daily intake will be less than 20 mg. The determination of aluminium in blood plasma of electrolysis-workers by flameless atomic absorption spectrometry revealed levels before shift in the range of 20–30 g/L compared to levels of less than 3 g/L in a non-occupationally exposed group. These levels refer to work in old plants with unhooded pots. The plasma levels can rise up to 60 g/L during the shift. A similar rapid change of the levels during work is seen in the urine values. Due to the delay in excretion the maximal values in the range of 5 g/h are found four hours after cessation of work. It seems therefore that only preshift samples give a reliable measure of the body burden. Compared to the much higher levels in dialysis patients the observed values in aluminium workers are not considered to be of relevance to health.     

Use of 15N-depleted artificial compost in bound residue studies

Association of bound residues to soil humic matter may be accomplished by different binding mechanisms such as sequestration in hydrophobic interiors of the organic material or covalent linkage to the organic matter. The structures and chemical environments of compounds can be observed by NMR spectroscopy. We applied 15N-NMR spectroscopy to study the soil-bound residues of 15N-labeled simazine. As the 15N-isotope has a low sensitivity and natural abundance 15N-NMR experiments require long measurement times and often result in low signal-to-noise (S/N) ratios. Therefore, in addition to the use of 15N-labeled simazine, 15N-depleted compost was used to reduce the amount of background signal and enhance the sensitivity. The compost was produced from maize and wheat plants grown on sand with 15N-depleted NH4NO3 as sole nitrogen source. The plants were freeze-dried, ground and mixed with sand for composting. After a composting period of 224 days analysis of the compost revealed a 15N-content of 267 ppm as opposed to a natural abundance of 3650 ppm. Characterization of this artificial compost produced parameter values similar to those of a natural compost. The 13C-NMR-spectra of the humic and fulvic acids during different stages of maturity showed that there was a shift from single-bond functional groups to more complex double-bond and aromatic structures. Experiments with this compost showed an increased signal intensity. The improved sensitivity made it possible to obtain interpretable NMR signals in contrast to experiments with 15N-simazine on native soil where no signals were detectable. The data indicated that the bound residues of simazine are composed of metabolites resulting from N-dealkylation and triazine ring destruction. Silylation of the bound residues showed a very strong binding of the residues to the matrix as only a small fraction could be solubilized.     

Biomonitoring of persistent organochlorine pesticides, PCDD/PCDFs and dioxin-like PCBs in blood of children from South West Germany (Baden-Wuerttemberg) from 1993 to 2003

In the context of a monitoring program, persistent organic pollutants (POPs) were quantified in the blood of 10 year old children at four different demographic regions in Baden-Wuerttemberg, a highly industrialised federal state in South West Germany. DDE, HCB, PCB 138, PCB 153 and PCB 180 were measured in 1996/1997, 1998/1999, 2000/2001 and 2002/2003 in individual samples of about 400 children per year. PCDD/PCDFs and some relevant coplanar PCBs were determined in pooled samples from children in seven cycles from 1993 to 2003. Blood concentrations of the investigated compounds decreased in that time period by a factor of 2-4 with the exception of most PCDFs. The concentrations of POPs in the blood of the children were distinctly lower than the concentrations reported for adults. Breast feeding was associated with about 30% higher median concentrations of DDE, HCB, PCBs and a 30% increase for mean PCDD/PCDF concentrations. Concerning demographic differences, significant lower concentrations of HCB, PCBs and PCDD/PCDFs could be seen in children from Mannheim compared to the region of Aulendorf. About 10-20% higher concentrations were found in boys compared to girls for HCB, indicator PCBs and PCDD/PCDFs. The pattern of non-ortho and mono-ortho PCBs in the blood of children was similar to the pattern reported for mother's milk, and PCB 126 and PCB 156 contributed about 70% to the toxicity of dioxin-like PCBs and about one-third to total TEQ including PCDD/PCDFs.     

A comparative study of the costs of alternative mayfly oviposition behaviors

Oviposition behavior of insects has associated fitness costs related to the probability that females survive to oviposit. During summer 2003, we observed the oviposition behavior and compared the mortality rates of females of 17 mayfly species in one western Colorado watershed. We dissected adult females collected on terrestrial sticky traps, in drift nets submerged in streams, and in stomachs of brook trout to determine whether the mayflies had oviposited before capture, drowning, or consumption. Females oviposited by either splashing on the water surface releasing all their eggs (splashers), dropping their eggs from the air (bombers), dipping their abdomens multiple times releasing a few eggs at a time (dippers), landing on rocks and ovipositing on the undersides (landers), or floating downstream while releasing their eggs (floaters). Almost 100% of lander and 50% of dipper females had not oviposited when captured on sticky traps, increasing their vulnerability to preoviposition mortality by aerial predators compared to mayflies with other behaviors. In contrast, most females had laid their eggs before drowning or being eaten by a fish (50–90%). However, groups with oviposition behaviors most exposed to the water surface (floaters, then splashers, dippers, and landers) were more vulnerable to drowning before completing oviposition. In addition, splashers and floaters were most vulnerable to predation by brook trout before ovipositing. These data suggest that fitness costs associated with preoviposition mortality may be considerable depending on mayfly oviposition behavior. Furthermore, previously demonstrated benefits of low predation rates on eggs of lander species may be offset in part by costs to female survival. Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.     

Spatial variability in reproductive cycle of the gorgonians <Emphasis Type="Italic">Paramuricea clavata</Emphasis> and <Emphasis Type="Italic">Eunicella singularis</Emphasis> (Anthozoa,Octocorallia) in the Western Mediterranean Sea

Paramuricea clavata (Risso, 1826) and Eunicella singularis (Esper, 1794) are the most representative gorgonian species in hard bottoms sublittoral communities in the Western Mediterranean Sea. Reproductive cycles of two populations of both species were studied in two distinct locations approximately 600 km apart (Medes Islands and Cape of Palos), in order to assess interpopulation variability on a relevant geographic scale. Seasonal variation of lipid concentration levels in the gorgonian tissue was used as a tool to quantify energy storage by each studied population in order to explain possible interpopulation differences in gonadal output. Sex ratio in Medes Islands populations of both species was 1:1, while in Cape of Palos sex ratio was significantly male biased (1:7) in P. clavata, and female biased (1.7:1) in E. singularis populations. Spawning timing occurred in all cases coinciding with a marked increase in sea-water temperature in spring, and after the most successful feeding season, but comparing localities there was a clear temporal shift in the time of gametes release, appearing well linked to the shift in sea-water temperature rising in spring in both sites at the depth where populations are placed. Therefore, in this study the temperature appears as the main synchronizing factor of gonadal development within these populations. Significant differences in gonadal volume per polyp were found in both species owing mainly to differences in the number of gonads per polyp between populations, with Cape of Palos populations displaying higher values in both studied species, suggesting that the exposition to different local conditions may be reverted in a different gonadal output. But the observed patterns in lipid concentrations levels in gorgonians disable us to conclude that lipid concentration levels explain the observed differences in gonadal output found in this study.     

Winter migration of magellanic penguins (Spheniscus magellanicus) from the southernmost distributional range

A total of 22 magellanic penguins (Spheniscus magellanicus) from Isla Martillo in the Beagle Channel, Argentina, were successfully satellite tracked in 2004 (n = 7), 2005 (n = 7) and 2006 (n = 8) to monitor their winter migration after moult. Only one magellanic penguin migrated northwards into the Pacific Ocean, whereas all others remained in the Atlantic Ocean. In general, these birds left the island in an easterly direction, rounded Cabo San Diego, the southeasterly tip of South America, and continued northwards occupying inshore waters mostly less than 50 km from the coast, only occasionally venturing further offshore. By the end of the transmission period, birds were still travelling northwards and the most northerly positions were obtained from birds located in the area of Peninsula Valdés, Argentina, at a latitude of around 42°S, some 1,500 km from their breeding site on Isla Martillo. The mean maximum distance to the breeding site was, however, only 624 ± 460 km. The mean minimum distance covered during the study period was 1,440 ± 685 km, which corresponded to a mean distance of 23.2 ± 6.6 km covered per day. The northbound migration of the penguins could be separated into periods of rapid movement, interspersed with periods during which the birds remained for some time in particular coastal regions. Areas with a high density of daily penguin positions were observed in three distinct areas: at the northeastern coast of Tierra del Fuego, at the southern entrance of Golfo San Jorge and to the northeast of the Peninsula Valdez. The observed migration pattern is presumably driven by the formation and subsequent dispersal of areas of enhanced productivity as the season progressed. Our findings also suggest that magellanic penguins are increasingly threatened by human activities in coastal areas as penguins migrate northwards.