Large-Scale Survey of Human Enteroviruses in Wastewater Treatment Plants of a Metropolitan Area of Southern Italy

Human enteroviruses (HEVs) occur in high concentrations in wastewater and can contaminate receiving environmental waters, constituting a major cause of acute waterborne disease worldwide. In this study, we investigated the relative abundance, occurrence, and seasonal distribution of polio and other enteroviruses at three wastewater treatment plants (WWTPs) in Naples, Southern Italy, from January 2010 to December 2014. Influent and effluent samples from the three WWTPs were collected monthly. One hundred and sixty-one of the 731 wastewater samples collected (22.0%) before and after water treatment were CPE positive on RD cells; while no samples were positive on L20B cells from any WWTPs. Among the 140 non-polio enterovirus isolated from inlet sewage, 69.3% were Coxsackieviruses type B and 30.7% were Echoviruses. Among these, CVB3 and CVB5 were most prevalent, followed by CVB4 and Echo6. The twenty-one samples tested after treatment contained 6 CVB4, 5 CVB3, 3 Echo11, and 2 Echo6; while other serotypes were isolated less frequently. Data on viral detection in treated effluents of WWTPs confirmed the potential environmental contamination by HEVs and could be useful to establish standards for policies on wastewater management.     

Distribution and speciation of metals in annual rings of black willow

Information on the spatial distribution and speciation of metals in nonhyperaccumulator plants is lacking. This study used synchrotron X-ray fluorescence (SXRF) compositional imaging to investigate the spatial distribution of Ni, Mn, Cu, Zn, and Fe in annual rings of black willow (Salix nigra L.) collected from a metal-contaminated area, and used X-ray absorption spectroscopy (XAS) to investigate Ni and Mn speciation in regions of the annual rings with elevated Ni concentrations. Annual rings were recollected in early 2003 from an individual known to be enriched with Ni from previous studies. Compositional imaging showed Ni and associated co-contaminants conservatively located in an annual ring. When compared with a corresponding photomicrograph, SXRF compositional images showed that metals were sharply constrained by the boundaries of the annual ring, indicating a sudden onset and cessation of uptake, and a lack of post-growth mobility of the metals. There was a particularly strong correlation between Ni and Mn in the metal-enriched annual ring (r = 0.8822), which suggested similar transport and binding behavior of these elements. X-ray absorption spectroscopy showed Ni and Mn to be present in the 2+ oxidation state. X-ray absorption near edge structure spectroscopy (XANES) fingerprinting of localized, highly Ni-enriched regions within the lumen of willow xylem vessels found similarities with Ni-pectic acid complexes, Ni-histidine, and NiSO4.     

MODELING THE PERFORMANCE OF DEEP WASTE STABILIZATION PONDS1

ABSTRACT: The performance characteristics of a deep waste stabilization pond operating in Southeast Spain were studied for one year; the results show a high reduction in organic load and nutrients, as well as disinfection of the effluent, after a retention time that varies as a function of climatic conditions. Mathematical models describing its hydraulic, thermal, and biochemical bahavior are presented and compared with a set of measured data.     

Importance of legislation for maintaining forests on private properties in the Brazilian Cerrado

Environment, Development and Sustainability - This paper evaluates compliance with environmental laws in the municipality of Sorriso and the impact of changing legislation on vegetation. To verify...     

Land-cover changes and recent hydrological evolution in the Duero Basin (Spain)

Land-use and land-cover changes have attracted substantial scientific interest in recent years because of their marked influence on hydrological cycles. In developed countries of the Mediterranean basin, the generalized revegetation and forest growth in mountainous areas that occurred during the last five decades are negatively affecting the evolution of water resources in headwaters. In this study, changes in land cover in the Duero River Basin (northern Spain) during the last 50 years were analyzed and their role in hydrological evolution was estimated. For this purpose, step-wise linear regressions were developed to estimate the evolution of runoff as a function of climate (precipitation and temperatures). The results show a significant expansion of forest cover in the headwaters, although it has been more extensive in the mountains to the north of the basin than to the south. River discharges in the headwaters underwent a generalized decline during the study period (1961–2006), but precipitation over the same period did not show an appreciable trend. In the absence of noticeable trends in removal of water for human consumption, our results indicate that revegetation is contributing to the observed hydrological decline. Our hypothesis is confirmed because of the greater divergence in the evolution of precipitation and runoff in the northern headwaters (more forest growth) than in the south headwaters (less forest growth). Results suggest that further increases in forest area will enhance hydrological decline and highlight the importance of integrating land-cover information in water availability assessments in a region where water is a strategic resource.     

Assessing coastal vulnerability to climate change: comparing segmentation at global and regional scales

Recent concerns about potential climate-change effects on coastal systems require the application of vulnerability assessment tools in order to define suitable adaptation strategies and improve coastal zone management effectiveness. In fact, while various research efforts were devoted to evaluate coastal vulnerability to climate change on a national to global level, fewer applications were carried out so far to develop more comprehensive and site-specific vulnerability assessments suitable to plan possible adaptation measures at the regional scale. In this respect, specific indicators are needed to address climate-change-related issues for coastal zones and to identify vulnerable areas at the regional level. Two sets of coastal vulnerability indicators were selected, one for regional and one for global studies, respectively, concerning the same features of coastal systems, including topography and slope, geomorphological characteristics, presence and distribution of wetlands and vegetation cover, density of coastal population and number of coastal inhabitants. The proposed set of indicators for the regional scale was chosen taking into account the availability of environmental and territorial data for the whole coastal area of the Veneto region and was based on site-specific datasets characterized by a spatial resolution appropriate for a regional analysis. Moreover, a GIS-based segmentation procedure was applied to divide the coastline into linear segments, homogeneous in terms of vulnerability to climate change and sea-level rise at the regional scale. This approach allowed to divide the Veneto shoreline into 140 segments with an average length of about 1 km, while the global scale approach identified four coastal segments with an average length of about 66 km. The performed comparison indicated how the more detailed approach adopted at the regional scale is essential to understand and manage the complexities of the specific study area. In fact, the 25-m DEM employed at the regional scale provided a more accurate differentiation of the coastal area's elevation and thus of coastal susceptibility to the inundation risks, compared to the 1-km DEM used at the global level. Moreover, at the regional level the use of a 1:20,000 geomorphological map allowed to differentiate the unique landform class detected at the global level (e.g., fluvial plain) in a variety of more detailed coastal typologies (e.g., open coast eroding sandy shores backed by bedrock) characterized by a different sensitivity to climate change and sea-level rise. Accordingly, the information provided by regional indicators can support decision-makers in improving the management of coastal resources by considering the potential impacts of climate change and in the definition of appropriate actions to reduce inundation risks, to avoid the potential loss of valuable wetlands and vegetation and to plan the nourishment of sandy beaches subject to erosion processes.     

Olfaction in the female sheep botfly

The nasal botfly Oestrus ovis (Diptera, Cyclorrhapha: Oestridae) is a myiasis-causing insect species, which affects the health of sheep, goats and humans. Gravid females are viviparous and larviposit into the animal’s nostrils. Host-searching and larvipositing flies are visually guided and influenced by climatic conditions, whereas olfaction seemed to play no role in this process. However, here, we show that the antennae of adult O. ovis female flies are relatively small but well developed and inhabited by several types of olfactory sensilla. Further, we show that the antennal lobes of this species receive input from antennal afferents and consist of a clearly defined glomerular organisation. We also give the first evidence of the fly’s ability to detect several synthetic odour compounds. Our findings provide a morpho-functional basis for future investigations on olfactory-mediated behaviour of this insect pest.