Use of ATP Readings to Predict a Successful Hygiene Intervention in the Workplace to Reduce the Spread of Viruses on Fomites

The purpose of this study was to validate the use of adenosine triphosphate (ATP) for evaluating hygiene intervention effectiveness in reducing viral dissemination in an office environment. The bacterial virus MS-2 was used to evaluate two scenarios, one where the hand of an individual was contaminated and another where a fomite was contaminated. MS-2 was selected as a model because its shape and size are similar to many human pathogenic viruses. Two separate experiments were conducted, one in which the entrance door push plate was inoculated and the other in which the hand of one selected employee was inoculated. In both scenarios, 54 selected surfaces in the office were tested to assess the dissemination of the virus within the office. Associated surface contamination was also measured employing an ATP meter. More than half of the tested hands and surfaces in the office were contaminated with MS-2 within 4 h. Next, an intervention was conducted, and each scenario was repeated. Half of the participating employees were provided hand sanitizer, facial tissues, and disinfecting wipes, and were instructed in their use. A significant (p < 0.05) reduction was observed in the number of surfaces contaminated with virus. This reduction in viral spread was evident from the results of both viral culture and the surface ATP measurements, although there was no direct correlation between ATP measurements with respect to viral concentration. Although ATP does not measure viruses, these results demonstrate that ATP measurements could be useful for evaluating the effectiveness of hygiene interventions aimed at preventing viral spread in the workplace.     

Norovirus GII.Pe Genotype: Tracking a Foodborne Outbreak on a Cruise Ship Through Molecular Epidemiology,Brazil, 2014

Norovirus (NoV) is recognized as the most common cause of foodborne outbreaks. In 2014, an outbreak of acute gastroenteritis occurred on a cruise ship in Brazil, and NoV became the suspected etiology. Here we present the molecular identification of the NoV strains and the use of sequence analysis to determine modes of virus transmission. Food (cream cheese, tuna salad, grilled fish, orange mousse, and vegetables soup) and clinical samples were analyzed by ELISA, conventional RT-PCR, qRT-PCR, and sequencing. Genogroup GII NoV was identified by ELISA and conventional RT-PCR in fecal samples from 5 of 12 patients tested (41.7%), and in the orange mousse food sample by conventional RT-PCR and qRT-PCR. Two fecal GII NoV samples and the orange mousse GII NoV sample were successfully genotyped as GII.Pe (ORF 1), revealed 98.0–98.8% identities among them, and shared phylogenetically distinct cluster. Establishing the source of a NoV outbreak can be a challenging task. In this report, the molecular analysis of the partial RdRp NoV gene provided a powerful tool for genotyping (GII.Pe) and tracking of outbreak-related samples. In addition, the same fast and simple extraction methods applied to clinical samples could be successfully used for complex food matrices, and have the potential to be introduced in routine laboratories for screening foods for presence of NoV.     

Capability development of local communities for project sustainability in afforestation/reforestation clean development mechanism

It has been recognized that the involvement of local community is essential to ensure the sustainability of A/R CDM (afforestation/reforestation clean development mechanism) project. This study verifies if the risks of non-permanence and leakage are addressed in a registered small scale A/R CDM project in Vietnam. Workshops, interviews, and a questionnaire survey of local villagers revealed that the project has caused a shortage of land for conventional activities such as grazing, fuel wood collection and shifting cultivation, and consequently posed the risks of project non-permanence and leakage. It is suggested that participation of all stakeholders in the community to the A/R CDM project beyond existing land tenure and adequate carbon benefit sharing according to the level of contribution to the project are required to reduce the risk of non permanence. To ensure the participation, the community should have capability such as consensus building and collective action. Leakage would be minimized if the community has alternative measures to the conventional activities before starting the project. We argue that it is necessary to first develop a community’s capabilities in the readiness phase of any A/R CDM project in order to reduce the risks for the project sustainability, and that new sources of funding are needed for this purpose.     

Building regional priorities in forests for development and adaptation to climate change in the Congo Basin

Indentifying common priorities in shared natural resource systems constitutes an important platform for implementing adaptation and a major step in sharing a common responsibility in addressing climate change. Predominated by discourses on REDD + (Reduced Emissions from Deforestation and Forest Degradation and conservation, sustainable management of forests and enhancement of forest carbon stocks in developing countries) with little emphasis on adaptation there is a risk of lack of policy measures in addressing climate change in the Congo Basin. Forest products and ecosystem services provide security portfolios for the predominantly rural communities, and play major roles in national development programmes in both revenue and employment opportunities. Thus, raising the profile of forests in the policy arena especially in the twin roles of addressing climate change in mitigation and adaptation and achieving resilient development is crucial. Within the framework of the Congo Basin Forests and Climate Change Adaptation project (COFCCA) project, science policy dialogue was conducted to identify and prioritize forest based sectors vulnerable to climate change but important to household livelihoods and national development. The goal of the prioritization process was for the development of intervention in forest as measures for climate change adaptation in Central Africa. Participants constituted a wide range of stakeholders (government, Non Governmental Organizations, research institutions, universities, community leaders, private sectors etc.) as representatives from three countries directly involved in the project: Cameroon, Central African Republic and Democratic Republic of Congo. Building on national priorities, four forest related sectors were identified as common priorities at the regional level for focus on climate change adaptation. These sectors included: (1) energy with emphasis on fuel wood and Charcoal; (2) Water principally quality, quantity, accessibility, etc.; (3) Food with emphasis on Non Timber Forest Products, and (4) Health linked to healthcare products (medicinal plants). Using these prioritized sectors, the project focused on addressing the impacts of climate change on local communities and the development of adaptation strategies in the three pilot countries of the Congo Basin region. The four sectors constitute the key for development in the region and equally considered as priority sectors in the poverty reduction papers. Focused research on these sectors can help to inject the role of forests in national and local development and their potentials contributions to climate change adaptation in national and public discourses. Mainstreaming forest for climate change adaptation into national development planning is the key to improve policy coherence and effectiveness in forest management in the region.     

A review of myrmecophily in ant nest beetles (Coleoptera: Carabidae: Paussinae): linking early observations with recent findings

Myrmecophily provides various examples of how social structures can be overcome to exploit vast and well-protected resources. Ant nest beetles (Paussinae) are particularly well suited for ecological and evolutionary considerations in the context of association with ants because life habits within the subfamily range from free-living and predatory in basal taxa to obligatory myrmecophily in derived Paussini. Adult Paussini are accepted in the ant society, although parasitising the colony by preying on ant brood. Host species mainly belong to the ant families Myrmicinae and Formicinae, but at least several paussine genera are not host-specific. Morphological adaptations, such as special glands and associated tufts of hair (trichomes), characterise Paussini as typical myrmecophiles and lead to two different strategical types of body shape: while certain Paussini rely on the protective type with less exposed extremities, other genera access ant colonies using glandular secretions and trichomes (symphile type). We compare these adaptations with other taxonomic groups of insects by joining contemporary research and early sources and discuss the possibility of an attracting or appeasing effect of the secretion. Species that are ignored by their host ants might use chemical mimicry instead. Furthermore, vibrational signals may contribute to ant–beetle communication, and chemical signals have proven to play a role in host finding. The powerful defense chemistry of paussines as “bombardier beetles” is not used in contact with host ants. We attempt to trace the evolution of myrmecophily in paussines by reviewing important aspects of the association between paussine beetles and ants, i.e. morphological and potential chemical adaptations, life cycle, host specificity, alimentation, parasitism and sound production.

Settlement decisions in blue tits: difference in the use of social information according to age and individual success

Dispersers are expected to assess breeding habitat quality before settlement. Although cues reflecting habitat quality are well studied, social cues have not been as well evaluated. In this paper, we studied breeding habitat selection during 3 years in a natural population of blue tits, Cyanistes caeruleus, breeding in nest-boxes. Our aim was to investigate if this species used conspecific density and/or reproductive success of con- and heterospecifics (i.e., social cues) in settlement decisions. The patterns observed were consistent with the idea that juveniles, when dispersing from their natal patches, did not react to any of the cues that we tested. In contrast, breeders that dispersed seemed to respond to both conspecific mean patch reproductive success (PRS) and breeding density of the settlement patch in the year of dispersal, their response differing according to their own reproductive success. Indeed, failed breeders moved to areas with high PRS and low density relative to source patches, while successful breeders behaved the opposite. The comparison between juveniles and adults might be modulated by the limited time available to juveniles to gather information on PRS and density at the end of the dispersing year. Adults lacking these time constraints, however, seemed to rely on these conspecific cues although limited by their own quality. Additionally, breeders were more likely to be immigrants in patches with relatively low breeding success and density the previous year, suggesting that settlement is influenced by multiple cues, which may reveal information on different aspects of habitat and be available at different moments. Collectively, our results support the importance of social cues for blue tits’ settlement.     

Artiodactyls from the Pondaung Formation (Myanmar): new data and reevaluation of the South Asian Faunal Province during the Middle Eocene

Although Asia is thought to have played a critical role in the radiation of artiodactyls, the fossil record of stem selenodonts (“dichobunoids”) remains dramatically poor in tropical Asian regions. In this study, we report a new dichobunid genus and species Cadutherium kyaukmagyii and a new basal ruminant genus and species Irrawadymeryx pondaungi, from the late Middle Eocene Pondaung Formation, Central Myanmar. Although the scarcity of the present material prevents any attempts to investigate the phylogenetic relationships of Cadutherium with contemporaneous forms from other Holarctic landmasses, this new form shed new light on the diversity of these small rabbit-like ungulates during a key period of their evolutionary history. Reexamination of the small-bodied artiodactyls from Pondaung leads us to propose new identifications of certain published specimens and, in turn, to investigate the temporal and geographic distribution of taxa recognized in the Pondaung Formation. Although fragmentary, these potential new taxa reveal an unsuspected diversity of small forms among artiodactyls of Pondaung. This addition to the Eocene record of dichobunoids and early ruminants provides further insight in the diversity of dental patterns among small artiodactyls from the Pondaung Formation and attests to the antiquity of these groups in Southeast Asia.