Forensic fingerprinting and source identification of the 2009 Sarnia (Ontario) oil spill

This paper presents a case study in which integrated forensic oil fingerprinting and data interpretation techniques were used to characterize the chemical compositions and determine the source of the 2009 Sarnia (Ontario) oil spill incident. The diagnostic fingerprinting techniques include determination of hydrocarbon groups and semi-quantitative product-type screening via gas chromatography (GC), analysis of oil-characteristic biomarkers and the extended suite of parent and alkylated PAH (polycyclic aromatic hydrocarbon) homologous series via gas chromatography-mass spectrometry (GC-MS), determination and comparison of a variety of diagnostic ratios of "source-specific marker" compounds, and determination of the weathering degree of the spilled oil, and whether the spilled oil hydrocarbons have been mixed with any other "background" chemicals (biogenic and/or pyrogenic hydrocarbons). The detailed chemical fingerprinting data and results reveal the following: (1) all four samples are mixtures of diesel and lubricating oil with varying percentages of diesel to lube oil. Both samples 1460 and 1462 are majority diesel-range oil mixed with a smaller portion of lube oil. Sample 1461 contains slightly less diesel-range oil. Sample 1463 is majority lubricating-range oil. (2) The diesel in the four diesel/lube oil mixture samples was most likely the same diesel and from the same source. (3) The spill sample 1460 and the suspected-source sample 1462 have nearly identical concentrations and distribution patterns of target analytes including TPHs, n-alkane, PAHs and biomarker compounds; and have nearly identical diagnostic ratios of target compounds as well. Furthermore, a perfect "positive match" correlation line (with all normalized ratio data points falling into the straight correlation line) is clearly demonstrated. It is concluded that the spill oil water sample 1460 (#1, from the water around the vessel enclosed by a boom) matches with the suspected source sample 1462 (#3, from the vessel engine room bilge pump). (4) From the n-alkane and PAH analysis, it appears that the oil in the spill sample 1460 is slightly more weathered in comparison with sample 1462. The minor differences in fingerprints of two samples were most likely caused by weathering effects. (5) Sample 1461 (#2, from the vessel engine room bilge) and sample 1463 (#4, from the vessel bilge waste collection tank) demonstrated significantly different fingerprints and diagnostic ratios of target compounds from that of spill sample 1460. This was caused most likely by percentages of diesel to lube oil in these two samples different from that in spill sample 1460.     

Monitoring and adaptive management: resolving social and organisational issues to improve information sharing in natural resource management

Adaptive, or 'learning by doing', approaches are often advocated as a means of providing increased understanding within natural resource management. However, a number of organisational and social issues need to be resolved if these approaches are to be used successfully. A case study in the South Island high country of New Zealand is used to review what is needed to support an ongoing community-based monitoring and adaptive management programme. First, the case study is described, paying attention to the social context of the resource management problem. The results of a workshop that explored this problem are then outlined, along with a proposed information flow suggested by participants. Requirements for future steps to resolve these problems (such as information protocols and a multi-stakeholder information system) are discussed. Finally, some broad lessons are drawn from this exercise that could help others developing similar approaches.     

Evaluating the use of occupational standards for controlling toxic air pollutants

Currently, the U.S. Environmental Protection Agency has established air standards for relatively few chemicals. As a result, state agencies are faced with controlling air contaminants for a large number of chemicals posing potential public health threats. Use of occupational standards as a basis for deriving ambient air guidelines is a method used by states to control air toxics. This standard development approach is reviewed by considering the differences in the health basis and numerical values which often occur among the occupational standards set by OSHA, NIOSH and ACGIH. This study indicated that careful selection should be made of the most appropriate occupational standard to use to protect public health. A comparison is made of chemicals regulated by various state air toxic programs using occupational standards lowered by a safety factor to concentrations established by the U.S. EPA. It was found that the air guidelines vary, but the degree of stringency can be comparable.     

Genetic evidence of multiple parentage in broods of cliff swallows

Summary Parental exclusion analyses based on allozyme data were performed on 105 families of cliff swallows (Hirundo pyrrhonota) from southwestern Nebraska, USA. The protein products of seve polymorphic loci were resolved from blood, and at least one parental exclusion occurred at six of these loci. One or both putative parents were excluded for 35 nestlings from 22 different families. The mean number of non-kin nestlings in these families was 1.59. Non-kin nestlings were found in families with brood sizes ranging from two to five. A greater percentage of families in an 1100-nest colony had non-kin offspring than in two smaller colonics, although the difference was not statistically significant. Application of genetic models to these data and the observed distribution of parental exclusions suggested that multiple parentage in cliff swallows results more often from intraspecific brood parasitism than from forced extra-pair copulations. Based on the calculated probabilities of detecting non-kin, we estimate that 23.7% of all nestlings in our population are not the offspring of one or both of their putative parents. We estimate that about 43% of all cliff swallow nests in Nebraska contain at least one offspring resulting from intraspecific brood parasitism, and that about 6% of nests might contain offspring resulting from extra-pair copulations.     

Short femur length associated with severe intrauterine growth retardation

Four fetuses with a femur measuring greater than two standard deviations below the mean compared with the biparietal diameter are presented. These four fetuses were subsequently determined to have severe intrauterine growth retardation and no evidence of skeletal dysplasias.     

Prenatal detection of non-cardiac rhabdomyosarcoma

The most prevalent soft tissue tumour in children is rhabdomyosarcoma. These tumours may develop within or outside of muscle anywhere in the body and at any age. We report what is apparently the earliest case of non-cardiac rhabdomyosarcoma detected prenatally.     

Immunity in a Social Insect

 Although pathogens appear to have exerted significant selective pressure on various aspects of sociality, mechanisms of disease resistance in the social insects are poorly understood. We report here on an immune response to infection by the dampwood termite, Zootermopsis angusticollis. Nymphs immunized with an injection of 7.6×10⁷, 7.6×10⁵, or 7.6×10⁴ cells/ml glutaraldehyde-killed solution of the bacterium Pseudomonas aeruginosa had significantly higher survivorship than controls following a challenge with a lethal concentration of active bacteria. Similarly, nymphs exposed to a 9×10^–1 spores/ml suspension of the fungus Metarhizium anisopliae had higher survivorship than controls after a challenge with a lethal concentration of spores. Prior exposure to a pathogen thus conferred upon termites a degree of protection during a subsequent encounter with the same pathogen. This represents the first demonstration of immune function in vivo in a social insect. Received: 18 May 1999 / Accepted in revised form: 26 August 1999     

Estimating baseline carbon emissions for the Eastern Panama Canal watershed

To participate in the potential market for carbon credits based on changes in the use and management of the land, one needs to identify opportunities and implement land-use based emissions reductions or sequestration projects. A key requirement of land-based carbon (C) projects is that any activity developed for generating C benefits must be additional to business-as-usual. A rule-based model was developed and used that estimates changes in land-use and subsequent carbon emissions over the next twenty years using the Eastern Panama Canal Watershed (EPCW) as a case study. These projections of changes in C stocks serve as a baseline to identify where opportunities exist for implementing projects to generate potential C credits and to position Panama to be able to participate in the emerging C market by developing a baseline under scenarios of business-as-usual and new-road development. The projections show that the highest percent change in land use for the new-road scenario compared to the business-as-usual scenario is for urban areas, and the greatest cause of C emission is from deforestation. Thus, the most effective way to reduce C emissions to the atmosphere in the EPCW is by reducing deforestation. In addition to affecting C emissions, reducing deforestation would also protect the soil and water resources of the EPCW. Yet, under the current framework of the Clean Development Mechanism (CDM), only credits arising from reforestation are allowed, which after 20 years of plantation establishment are not enough to offset the C emissions from the ongoing, albeit small, rate of deforestation in the EPCW. The study demonstrates the value of spatial regional projections of changes in land cover and C stocks: The approach helps a country identify its potential greenhouse gas (GHG) emission liabilities into the future and provides opportunity for the country to plan alternative development pathways. It could be used by potential project developers to identify which types of projects will generate the largest C benefits and provide the needed baseline against which a project is then evaluated. Spatial baselines, such as those presented here, can be used by governments to help identify development goals. The development of such a baseline, and its expansion to other vulnerable areas, well positions Panama to respond to the future market demand for C offsets. It is useful to compare the projected change in land cover under the business-as-usual scenario to the goals set by Law 21 for the year 2020. Suggested next steps for analysis includeusing the modeling approach to exploreland-use, C dynamics and management ofsecondary forests and plantations, soilC gains or losses, sources ofvariability in the land use and Cstock projections, and other ecologicalimplications and feedbacks resulting fromprojected changes in land cover.     

Occurrence of heavy metals in the blue shark Prionace glauca and selected pelagic in the N. E. Atlantic Ocean

Interactions between period and degree of pressure have been investigated in membrane and filament systems of gill tissue, skin, and muscles of Modiolus modiolus, Mytilus edulis (Baltic and North Seas) and Branchiostoma lanceolatum (Pallas) at the ultrastructural level. The results show the importance of time for the degree of structural disintegration at constant pressure. Destruction already occurs after 2 min of low-pressure treatment at 300 atm. After 10 min continued pressure at the same level, no further structural dis-organization (qualitative or quantitative) occurs, even when the time period is prolonged. For some organelles (i.e., cilia and microvilli), better structural preservation is apparent after 30-min pressure compared to 10-min treatment at the same pressure level. This is a so-called “acclimatisation” to pressure.